3,798 research outputs found
Screening of DTP Compound Libraries for CK2 Inhibitors with Focus on Natural Products
No full textVarious compound libraries of the Drug Therapeutic Program (DTP) of the NIH/NCI were screened against the catalytic subunit and the tetrameric holoenzyme of protein kinase CK2. Different IC50 values were obtained for the two CK2 molecules. In the case of nortangeretin, the IC50 value was 0.34 μM for the catalytic subunit and 15 μM for the holoenzyme. In the case of coumestrol, opposite results were obtained, i.e., high IC50 for the CK2α subunit (2.7 μM) and a lower IC50 value for the holoenzyme (0.19 μM). From the many compounds identified to inhibit CK2, we have selected 14 different compounds and listed them according to their CK2α/CK2 holoenzyme IC50 ratio. Four compounds were tested on a panel of seven cell lines revealing considerable differences in the degree of CK2 inhibition inside the cells.</p
Sixty Years of Community: St. Olaf Catholic Parish in Eau Claire, Wisconsin, 1952-2012
No full textThis paper will explore how the parish community of St. Olaf in Eau Claire, Wisconsin, established in 1952, reflects the Roman Catholic Church, specifically at the local, state, and national levels in the United States. It will also discuss the various changes that have occurred in the past 60 years of its history in terms of the various locations of worship for the members, the growth of the community outreach programs, and the effects of the Second Vatican Council. This ecumenical council was a meeting of Catholic bishops from around the whole that brought reform to the Catholic Church and affected the relationship of the Catholic Church to the world. The parish at St. Olaf has grown from having only 125 families in 1952 to over 1,000 families in 2012
Dissection of the dual function of the β-subunit of protein kinase CK2 (‘casein kinase-2’): a synthetic peptide reproducing the carboxyl-terminal domain mimicks the positive but not the negative effects of the whole protein
No full textThe dual function of the regulatory beta-subunit of protein kinase CK2 is highlighted by its ability to abolish calmodulin phosphorylation in contrast to its stimulatory effect on the phosphorylation of peptide substrates, Here we show that a synthetic peptide reproducing the C-terminal region of the beta-subunit (beta[170-215]) stimulates to a similar extent the phosphorylation of either the peptide substrate or calmodulin and also protects the catalytic alpha-subunit against thermal inactivation as efficiently as full-length beta-subunit. These data show that the positive and negative functions of the beta-subunit reside in physically separated domains and that the elements responsible for positive regulation are located in the C-terminal region
Synthesis of Triazenoazaindoles: a New Class of Triazenes with Antitumor Activity
No full textDespite improvements in the treatment and prevention of cancer, the number of new diagnoses continues to rise; this has fuelled substantial interest in the development of new and effective chemotherapeutic agents. Compounds of the triazene class, such as dacarbazine, have been used in the clinical management
of many cancer types including brain, leukemia, and melanoma. A new compound class bearing a triazenoazaindole
scaffold was synthesized with the aim of identifying new antiproliferative
agents. Compounds 5a–g and 6a–c were
screened against a panel of human tumor cell lines, and two of them, 5e and 5 f, showed cytotoxicity (GI50 range: 2.2–8.2 mm) in all cell lines. These two compounds even maintained their cytotoxicity in some multidrug-resistant cell lines. Flow cytometry analysis demonstrated their ability to induce cell death by apoptosis with involvement of lysosomes
Georg Hermann.
No full textThe internationally renowned author of numerous novels, essays, and articles, Georg Hermann, was born as Georg Borchardt in Berlin-Friedenau on October 7, 1871, the youngest of six children in a well-established Jewish family. Later in life he used his father’s first name Hermann as his surname when writing. Contrary to the expectations for a young man from a reputable family, Hermann did not pursue the Abitur exam in a Gymnasium (secondary school), but instead received a one-year certificate in 1890, leaving school to become an apprentice salesman at a tie company. From 1896 until 1899 he worked in the Statistical Office of Berlin, at the same time attending literature and art history lectures at the University of Berlin. Afterwards he worked as a freelance writer and art critic.His first book, 'Spielkinder', was published in 1896, but he did not become well-known until 1906, with the publication of 'Jettchen Gebert', followed by its sequel, 'Henriette Jacoby'. These novels told the story of the life of a young woman living in Jewish Berlin during the Biedermeier period of the 1820s and 1830s. Politically active, Georg Hermann was also a member of the Central-Verein deutscher Staatsbürger jüdischen Glaubens.Having become known for his pacifist tendencies through his writing, and because of his Jewish heritage, Georg Hermann and his family fled to Holland shortly after the burning of the Reichstag in 1933. Although the rest of his family was saved from the Nazis after their occupation of Holland in 1943, Georg Hermann was sent to the Dutch concentration camp of Westerbork. On November 16, 1943 he was transported to Auschwitz and either died during transport or shortly after his arrival.Digital ImageRecord added to DigiTool. Aleph record suppressed. J. Palmisano 09/15/2010
Northern Rover: The Life Story of Olaf Hanson
No full textFrom 1919 to 1970, Olaf Hanson was a trapper, fur trader, prospector, game guardian, fisherman, and road blasting expert in northeastern Saskatchewan. He told his life story to popular Saskatchewan author A. L. Karras, who wrote this historical memoir in the 1980s. In an uncompromising, straightforward style, Karras and Hanson reveal the geography, wildlife, natural history of the region as well as the business and social interactions between people. Their book offers a look at the vanished subsistence and commercial economy of the boreal forest, wound around a fascinating personal story of courage and physical stamina
Northern Rover: The Life Story of Olaf Hanson
No full textFrom 1919 to 1970, Olaf Hanson was a trapper, fur trader, prospector, game guardian, fisherman, and road blasting expert in northeastern Saskatchewan. He told his life story to popular Saskatchewan author A. L. Karras, who wrote this historical memoir in the 1980s. In an uncompromising, straightforward style, Karras and Hanson reveal the geography, wildlife, natural history of the region as well as the business and social interactions between people. Their book offers a look at the vanished subsistence and commercial economy of the boreal forest, wound around a fascinating personal story of courage and physical stamina
Sammlung von Merckwürdigkeiten der Natur und Alterthümern des Erdbodens, welche petrificirte Cörper enthält
No full textaufgewiesen und beschrieben von Georg Wolffgang KnorrVorlageform des Erscheinungsvermerks: Nürnberg, zu finden bey dem Author. Gedruckt bey Andreas Bieling
Portrait of author Olaf Ruhen, Bathurst, New South Wales, 1975 [picture] /
No full textTitle from inscription.; Inscription: 'Author Olaf Ruen [i.e. Ruhen], Bathurst, NSW, 1975"--Lower left.; Also available in an electronic version via the Internet at: http://nla.gov.au/nla.pic-vn3697108; Purchased from the photographer, 2006
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