1,720,965 research outputs found
Effect of Pb-acetate on Testicle Weight and Volume of Mice
Full text linkBackground: The human effort to meet the needs of human life is almost always cause pollution in the environment of his life. One of the pollutants that harm human beings are lead (Pb = plumbum), Pb is one of the heavy metals that are harmful to health. Pb may also affect the reproductive organs. The purpose of this study to determine the effect of Pb-acetate on mouse testis weight and volumeDesign and Method: Eighty male mice placed in individual cages according to the group. Food and drink provided ad libitum. The treatment of male mice given for 6 weeks. Treatment with 0.2 ml distilled water to PI, P II were given a solution of 400 ppm Pb acetate total of 0.2 ml, P III was given a solution of 1000 ppm Pb acetate as many as 0.2 ml, and P IV were given a solution of 2000 ppm Pb acetate total of 0,2 ml by using a gastric sonde, every morning once a day for 42 days. At week 7, mice were surgery to take the testis. Testicular volume was measured and weighed. Data were analyzed by ANOVA, hypothesis is accepted when p 0.05), testis weight obtained by ANOVA test probability of 0.216 (> 0.05), mean Pb -acetate had no effect on testis weight and volume.Conclusion: Pb-acetate had no effect on testis weight and volume (Sains Medika, 3(2):150-156)
PENGARUH PEMBERIAN JUS KECAMBAH KACANG HIJAU (Vigna radiata L) TERHADAP MORFOLOGI SPERMATOZOA PADA TIKUS JANTAN YANG DIPAPARKAN ASAP ROKOK
Full text linkMerokok ialah kebiasaan yang dapat menyebabkan berbagai macam penyakit seperti faringitis, penyakit paru obstruktif, kanker paru-paru dan penurunan sistem reproduksi pada pria. Kandungan yang terdapat pada rokok dapat meningkatan kadar Reactive Oxygen Species (ROS) didalam tubuh sehingga menimbulkan terjadinya abnormalitas morfologi spermatozoa. Pada masyarakat Indonesia kecambah kacang hijau dipercayai mampu memberikan manfaat yaitu kesuburan pada pria akan meningkat. Penelitian ini untuk mengetahui pemberian jus kecambah (Vigna radiata (L.) Wilczek) berefek terhadap morfologi spermatozoa pada tikus jantan yang dipaparkan asap rokok.
Metode penelitian ini adalah eksperimental dengan rancangan post test only control group design, menggunakan subjek uji tikus jantan sebanyak 30 ekor yang terbagi menjadi 6 kelompok yaitu kelompok (N), kelompok (K-), kelompok (K+), kelompok (P1), kelompok (P2), dan kelompok (P3). Subjek uji sebanyak 30 tikus dilakukan adaptasi selama 7 hari, kemudian diberikan perlakuan selama 28 hari, kemudian pada hari ke 29 dilakukan pembedahan dan pemeriksaan persentase morfologi spermatozoa.
Hasil penelitian menunjukan pada kelompok (N) menghasilkan persentase morfologi spermatozoa sebesar 37,33%, kelompok (K-) 21,10%, kelompok (K+) 35,20 , kelompok (P1) 25,36%, kelompok (P2) 29,46 % dan kelompok (P3) 36,34 %. Data hasil penelitian di uji normalitasnya dengan uji Shapiro-Wilk (p>0,05) menunjukan hasil yang normal, dan di uji homogenitasnya menggunakan uji Lavene Test (
The Effect of Propolis Extract on MDA Levels (Malondialdehyde) and Sperm Quality on Epididimis (Experimental Study on Wistar Strain Male Rats Exposed to Kretek Cigarettes)
Full text linkIntroduction: Cigarette smoke exposure increase free radicals and effecting male infertility. Propolis is commonly used as supplement to increase antioxidant levels. Objectives: To prove the effect of propolis extract to MDA level and spermatozoa quality on epididymis due to cigarette smokes. Methods: Experimental research post test only control group design using 30 male rats Wistar Strain, 8 weeks old, weighed 200 – 250 gram, randomly divided into 5 groups. K-1 (normal), do not received propolis and cigarettes smoke; K-2 (negative control) exposed to cigarettes smokes 4 buds/ day, did not received propolis; K-3, K-4, and K-5, apart from cigarrete smokes 4 buds/day each were given propolis with 2.9 mg, 5.4 mg, dan 8.3 mg/200g body weight/day for 21 days. On the 22nd day, rats were sacrificed, sperms were extracted from cauda epididymis, and then examined for MDA and sperm quality. Statistical analysis used was one way ANOVA, continued with post hoc LSD with significance level of p<0,05. Results: ANOVA test on MDA level and sperm quality between groups showed significant difference of, p = 0.005. Post hoc test indicated that MDA on K-5 (1,12) is significantly lower compare to K-2 (5,89), p = 0,001. As well as consentration, motility and morphology (spermatozoa quality) which indicated that K-5 (49.32, 64.15, 36.34) is significantly higher compared to K-2 (22.62, 27.27, 21.39), each with p value of p = 0.001.Conclusion: extract propolis 8,3 mg/day for 21 days is proven to increase spermatozoa quality and lower the MDA level on male rats wistar strains’ epididimis exposed to cigarettes smoke
The Effect of Continuous Multistage Countercurrent Extraction (CMCE) Propolis Extract Administration on Leydig, and Sertoli Cells Counts, and Sperm Quality Induced With Monosodium Glutamate (MSG) (An experimental study on male Wistar rat induced by monosodium glutamate)
Full text linkINTRODUCTION:Â ROS is the most common cause of male infertility. Monosodium glutamate (MSG) is a compound which produces ROS and decreases FSH and LH levels. Continuous Multistage Countercurrent Extraction (CMCE) propolis extract as an antioxidant is evidently capable of reducing ROS concentration.OBJECTIVE: this research aims at evaluating the effect of CMCE propolis extract administration on Leydig cells and Sertoli cells counts and sperm quality of MSG-induced male wistar rats.METHODS: employing the Post Test Only Control Group Design, 18 rats were divided into 3 groups: control group, treated only with MSG (MS-G), and treatment groups, respectively treated with CMCE propolis extract at doses 8.3mg/day (CM8-G) and 10.8mg/day (CM10-G). CMCE propolis extract was given orally for 21 days. At the end of treatment, sperm and testis were taken to evaluate the Leydig cells and Sertoli cells counts and sperm quality. The sperm obtained from epididymis was analyzed using the WHO standards, while Leydig cells and Sertoli cells were counted from preparation using the HE stain method.RESULTS: the Post Hoc analysis result shows that the Leydig and Sertoli cells counts and sperm quality of CM8-G and CM10-G are significantly higher than those of MS-G, p<0.05CONCLUSION: CMCE propolis extract administration evidently increases the Leydig cells and Sertoli cells counts and sperm quality from the perspective of the count, motility and morphology of spermatozoa of MSG-induced male wistar rats.Â
HIGH DOSE VITAMIN C ADMINISTRATION EFFECT IN LEYDIG CELLS, SERTOLI CELLS NUMBER, AND SPERM QUALITY ON MALE WISTAR RATS
Full text linkIntroduction: Most infertile male are associated with poor spermatogenesis due to oxidative stress, and can be prevented with vitamin C. However, excessive amount of high dose of vitamin C can hindered and lower the sperm quality. Objective: To prove that high dose vitamin C is capable to decrease the number of leydig cells, sertoli cells, and sperm quality on male wistar rats.Methods: This research was using experimental method with Post Test Only Controlled Group Design. Of 24 male Wistar rats, divided randomly to 4 groups. Normal groups (Nor -G), only given 2 ml/day distilled water; vitamin C group (VC18-G, VC36-G, and VC72-G) given 18 mg/day, 36 mg/day and 72 mg/day vitamin C respectively, dissolved in 2 ml of distilled water. Sperm, the number of Leydig cells and Sertoli cells were taken from the epididymis and left right testicle on day 21. Sperm analysis using WHO standard, while the number of Leydig cells and Sertoli cells with HE staining.Results: Mann Whitney analysis indicated that the number of sperm in VC36-G and VC72-G are lower compared to that of Nor-G and VC18-G groups, p <0.05. Post-Hoc LSD analysis showed that the lowest number of Leydig and Sertoli Cell, and the weakest sperm motility and morphology in VC36-G and VC72-G groups, compared to that of Nor-G and VC18-G groups, p <0.05 .Conclusion: Vitamin C 36 and 72 mg/day were capable of reducing Leydig and Sertoli cells number, and worsen sperm quality, characterized by decreased in sperm concentration, motility and morphology in Wistar male- rats.Introduction: Most infertile male are associated with poor spermatogenesis due to oxidative stress, and can be prevented with vitamin C. However, excessive amount of high dose of vitamin C can hindered and lower the sperm quality. Objective: To prove that high dose vitamin C is capable to decrease the number of leydig cells, sertoli cells, and sperm quality on male wistar rats.Methods: This research was using experimental method with Post Test Only Controlled Group Design. Of 24 male Wistar rats, divided randomly to 4 groups. Normal groups (Nor -G), only given 2 ml/day distilled water; vitamin C group (VC18-G, VC36-G, and VC72-G) given 18 mg/day, 36 mg/day and 72 mg/day vitamin C respectively, dissolved in 2 ml of distilled water. Sperm, the number of Leydig cells and Sertoli cells were taken from the epididymis and left right testicle on day 21. Sperm analysis using WHO standard, while the number of Leydig cells and Sertoli cells with HE staining.Results: Mann Whitney analysis indicated that the number of sperm in VC36-G and VC72-G are lower compared to that of Nor-G and VC18-G groups, p <0.05. Post-Hoc LSD analysis showed that the lowest number of Leydig and Sertoli Cell, and the weakest sperm motility and morphology in VC36-G and VC72-G groups, compared to that of Nor-G and VC18-G groups, p <0.05.Conclusion: Vitamin C 36 and 72 mg/day were capable of reducing Leydig and Sertoli cells number, and worsen sperm quality, characterized by decreased in sperm concentration, motility and morphology in Wistar male- rats
PENGARUH PEMBERIAN EKSTRAK BUAH NANAS MUDA TERHADAP KECACATAN FETUS MENCIT BETINA
Full text linkPendahuluan: kelainan kongenital memberikan distribusi sebagai salah satu penyebab yang cukup tinggi kematian pada bayi. Tiga janin dengan persentase 12,67% mengalami perdarahan setelah pemberian ekstrak buah nanas muda yaitu pada kerangka metakarpus dan metatarsus, hasil preparat menunjukkan keterlambatan proses pengerasan tulang karena konsentrasi enzim bromelain dalam kulit nanas 0,050-0,075%. Tujuan penelitian ini untuk mengetahui pengaruh pemberian ekstrak buah nanas muda terhadap panjang ekor, kelainan palatum durum, dan jumlah ruas jari kaki fetus mencit.
Metode: post test only control group design dilakukan pada 24 ekor mencit hamil, kelompok kontrol (K1) dengan pakan standar, K2 dengan dosis nanas muda 0,02 mg/0,1 ml aquadest/10 gBB, K3 dengan 0,04 mg/0,1 ml aquadest/10 gBB, K4 dengan dosis 0,08 mg/0,1 ml aquadest/10 gBB. Analisis dengan IBM Statistic SPSS 25 untuk melakukan uji hipotesis dengan Kruskall-walis dengan nilai p-valu
Red Ginger (Zingiber officinale var. Amarum) Infusion Increases Concentration of Luteinizing Hormone and Testosterone in Male Rats
Full text linkIntroduction: Herbal remedies have been developed as a complementary therapy to increase sex hormones. The administration of the ethanolic extract of red ginger (Zingiber officinale var. Amarum) has been shown to increase spermatogenic cells, motility and morphology of sperm in mice. However, the effect of red ginger infusion on the level of testosterone and luteinizing hormone (LH) has not been done. Objective: to examine the effect of red ginger infusion on LH and testosterone levels of male Wistar rats.Methods: The study was designed as an experimental post-test only control group design. Twenty four male Wistar rats of 200-250 g body weight aged + 3-4 months were divided into four groups. a control group (G-1) was given distilled water 2 ml/day. Group 2 (G-2), Group 3 (G-3), and group 4 (G-4) were treated with 10%, 20%, and 40% infusion by dissolving 2 ml of ginger in distilled water. On day 15, blood samples were taken and assayed for LH and testosterone.Results: The one-way ANOVA test showed difference in levels of LH and testosterone between groups (p <0.001). Post Hoc LSD test results showed that the levels of LH of G-1 (0350) was significantly lower than that of G-2 (1082), G-3 (1 253), and G-4 (0923), p <0.005. While the levels of testosterone in the G-1 (0.2718) significantly lower than the G-2 (0.7797), G-3 (0.8285), and the G-4 (0.9142) (p <0.005).Conclusion: red ginger infusion for 14 days can increase the levels of LH and testosterone in male rats
The Level of SGOT and SGPT after The Administration of Powdered Colorant Pigment Isolated from Kesumba Keling’s (Bixa Orellana) Seed Coats An experimental study in Balb/C Mice
Full text linkBackground: There has been an effort to explore pigment from kesumba keling’s (Bixa orellana) seed coats for a source of natural food colorant. Consumption of pigment from kesumba keling (Bixa orellana) was assumed to cause adverse effects on heart as an important organ in detoxification or inactivation of drugs or other toxic compounds. This study conducted to determine the levels of SGOT and SGPT blood serum of mice (Mus musculus) Balb/C after administration of colorant powder pigment of B. orellana seed coat’s at various concentrations.Design and Methods: The research is an experimental research with Factorial Design with 2 factors. Food Colorant Powder was made from bixin, norbixin, and crude extract pigment isolated from B. orellana seed coat’s. A total of 60 male mice were divided into 10 treatment groups. Powdered colorant solution at the concentration of 0% (control), 1%, 5%, and 10% were orally administered using gavage as much as 1 ml a day for 30 days.Results: Kruskal Wallis test results shows a not significant difference SGOT and SGPT level among the groups (p > 0.05), thus consumption of colorant powder of bixin, norbixin, and the crude extract with various concentrations did not affect the SGOT and SGPT.Conclusion: Colorant powder isolated from B. orellana seed coat’s has been shown to have no effect on the liver function, so it is safe as food colorant (Sains Medika, 3(1):69-77)
The Effect Honey Has on SOD and Caspase9 Levels with Exposure to Cigarette Smoke in Wistar Rats
Full text linkCigarettes are a source of exogenous free radicals which disrupt the balance between free radicals and antioxidants in the body, causing oxidative stress. The aim of this study was to determine the effect of giving honey (Mel depuratum) on SOD and caspase-9 levels in Wistar rats exposed to cigarette smoke. This research used a laboratory experimental approach with a post-test only control group design. 24 Wistar rats were divided randomly into 4 groups then analysis tests were carried out using the One-Way ANOVA test and followed by the Post-Hoc LSD test. The results showed that the mean SOD levels in groups K1, K2, K3, K4 were 73.51 ± 1.68, 21.72 ± 2.23, 52.08 ± 2.03, and 73.51 ± 1.68. The results of the One Way Anova test showed a significant difference in SOD levels with a value of p = 0.000. The results of the LSD Post-Hoc Test showed that SOD levels between groups had significant differences in all groups (p=0.000). The IHK staining results showed that samples K1 and K4 were negative, while K2 showed strong positive and K3 was moderately positive
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