853 research outputs found

    Isaria // Metallfadenlampe. Isaria Zahlerwerke. Mediengesescgahft. München.

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    ISARIA // METALLFADENLAMPE. ISARIA ZAHLERWERKE. MEDIENGESESCGAHFT. MÜNCHEN. Isaria // Metallfadenlampe. Isaria Zahlerwerke. Mediengesescgahft. München. ( -

    Secondary Metabolites and the Risks of <i>Isaria fumosorosea</i> and <i>Isaria farinosa</i>

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    Isaria fumosorosea and Isaria farinosa are important entomopathogenic fungi with a worldwide distribution and multiple host insects. However, the concerns about the safety risks of myco-pesticides have been attracting the attention of researchers and consumers. Secondary metabolites (SMs), especially the mycotoxins, closely affect the biosafety of Isaria myco-insecticides. In the last forty years, more than seventy SMs were identified and isolated from I. fumosorosea and I. farinose. The SMs of I. fumosorosea include the mycotoxins of non-ribosomal peptides (NRPs) (beauvericin and beauverolides), terpenes (trichocaranes and fumosorinone), lactone compounds (cepharosporolides), acids (dipicolinic acid and oxalic acid), etc. Meanwhile, the NRP mycotoxins (cycloaspeptides) and the terpene compounds (farinosones and militarinones) are the main SMs in I. farinosa. Although several researches reported the two Isaria have promised biosafety, the bioactivities and the safety risks of their SMs have not been studied in detail so far. However, based on existing knowledge, most SMs (i.e., mycotoxins) do not come from Isaria myco-insecticide itself, but are from the host insects infected by Isaria fungi, because only the hosts can provide the conditions for fungal proliferation. Furthermore, the SMs from Isaria fungi have a very limited possibility of entering into environments because many SMs are decomposed in insect cadavers. The biosafety of Isaria myco-insecticides and their SMs/mycotoxins are being monitored. Of course, SMs safety risks of Isaria myco-insecticides need further research

    Patogenicidade de Beauveria bassiana, Isaria javanica e I. farinosa sobre Tetranychus urticae

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    MonografiaBrazil bears the title of second largest producer of papaya, however, some problems limit the cultivation of papaya, such as frequent manifestations of mites. Among the mites that attack this crop, Tetranychus urticae Koch has spread causing great damage to the producers. Aiming at an alternative control method of this pest, the objective of this study was to verify the efficiency of different strains of the entomopathogenic fungi Beauveria bassiana and Isaria spp. in the biological control of Tetranychus urticae in vitro. In order to meet the objectives, the work was carried out from two bioassays: the first one on the still phases of the mite, with application of the treatments in the egg phase and evaluation until the adult stage; and the second on the mobile phases with application in the protoninfa phase and evaluation until the adult stage. The treatments were formulated from four fungal strains corresponding to Isaria facinora (URM1355), Isaria javanica (URM4995), Beauveria bassiana (URM202) and Beauveria bassiana from commercial sources for comparison with Spirodiclofen and Abamectin as controls. The fungi were grown in Potato-Dextrose-Agar (BDA) medium, in Petri dishes incubated in a BOD (Biological Oxygen Demand) chamber at 28 ± 1 ° C, RH 60 ± 10%, and were adjusted to the concentration of 1 x 108 conidia / ml, diluted in sterile water containing Tweem 80 (0.05% v / v), to be applied by simple spraying in plots containing 22 plates / treatment with 5 copies of organisms arranged in wet leaf discs. The treatments were maintained throughout the evaluation period in conditioned chambers (B.O.D.) at 25 ± 1ºC, with relative humidity (RH) of 60 ± 10% and photoperiod of 12 h. The bioassay of the immobile phases revealed that only the treatment with Spirodiclofen presented maximum lethal effect (100%), preventing the hatching of eggs. In addition to spirodiclofen, Beauveria bassiana commercial treatment also obtained lethality reaching 61% of the total deaths. The results showed that in addition to spirodiclofen, the Beauveria bassiana commercial treatment also obtained lethality reaching 61% of the total deaths. The bioassay of the mobile phases did not obtain significant results in any of the treatments to assert their efficiency, only Abamectin was effective in the control. Thus, it can be concluded that only the commercial fungal line of B. bassiana presents a lethal effect to the mite under the conditions tested.O Brasil porta o título de segundo maior produtor mundial de mamão, contudo, alguns problemas limitam o cultivo do mamoeiro, como as frequentes manifestações de ácaro. Dentre os ácaros que acometem essa cultura, Tetranychus urticae Koch tem se disseminado causando grandes prejuízos aos produtores. Visando um método de controle alternativo dessa praga, o objetivo desse estudo foi verificar a eficiência de diferentes linhagens dos fungos entomopatogênicos Beauveria bassiana e Isaria spp. no controle biológico de Tetranychus urticae in vitro. Para atender aos objetivos, o trabalho foi realizado a partir de dois bioensaios: o primeiro sobre as fases imóveis do ácaro, com aplicação dos tratamentos na fase de ovo e avaliação até a fase adulta; e o segundo sobre as fases móveis com aplicação na fase de protoninfa e avaliação até a fase adulta. Os tratamentos foram formulados a partir de quatro linhagens fúngicas que correspondiam a Isaria facínora (URM1355), Isaria javanica (URM4995), Beauveria bassiana (URM202) e Beauveria bassiana de fontes comercias para comparação com Espirodiclofeno e Abamectina como testemunhas. Os fungos foram crescidos em meio Batata-Dextrose-Ágar (BDA), em placas de Petri incubadas em câmara BOD (Biological Oxigen Demand), a 28 ± 1º C, UR 60 ± 10%, e foram ajustados para a concentração de 1 x 108 conídios/mL, diluídos em água estéril contendo Tweem 80 (0,05% v/v), para serem aplicados por pulverização simples em parcelas contendo 22 placas/ tratamento com 5 exemplares de organismos dispostos em discos foliares úmidos. Os tratamentos foram mantidos durante todo período de avaliação em câmaras climatizadas (B.O.D.) a 25±1ºC, com umidade relativa (UR) de 60±10% e fotoperíodo de 12 h. O bioensaio das fases imóveis revelou que apenas o tratamento com Espirodiclofeno apresentou efeito máximo letal (100%), impedindo a eclosão de ovos. Os tratamentos de origem fungíca não obtiveram efeito inibitório da eclosão de ovos, já em relação a mortalidade, os resultados mostram que além de Espirodiclofeno, o tratamento Beauveria bassiana comercial também obteve letalidade chegando a 61% do total de mortes. O bioensaio das fases móveis não obteve resultados significativos em nenhum dos tratamentos para se afirmar a eficiência dos mesmos, apenas Abamectina mostrou-se eficaz no controle. Assim, pode-se concluir que apenas a linhagem fúngica comercial de B. bassiana apresenta efeito letal ao ácaro, nas condições testadas.Trabalho não financiado por agência de fomento, ou autofinanciad

    Ocena strat zarodników grzyba Isaria fumosorosea w mieszadle hydraulicznym

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    The study was conducted assessing the losses of spores of the fungus species Isaria fumosorosea during the flow of liquid through the nozzle of the hydraulic agitator. The liquid flow containing spores was 2.5 dm3/min and the pressure 6.0 bar. The liquid circulated 100 times in the system comprising a pump, hydraulic mixer, and reservoir. It has been found that the multiplicity of fluid flow has a significant effect on reducing the number of spores of the fungus Isaria fumosorosea contained in a liquid. The maximum reduction of spores was 89%.Przeprowadzono badania oceny strat zarodników grzyba gatunku Isaria fumosorosea podczas przepływu cieczy przez dyszę mieszadła hydraulicznego. Natężenie przepływu cieczy zawierającej zarodniki wynosiło 2,5 dm3/min, a jej ciśnienie 6,0 bar. Ciecz krążyła w instalacji (składającej się z pompy, mieszadła hydraulicznego i zbiornika) do 100 razy. Stwierdzono, że krotność przepływu cieczy ma istotny wpływ na redukcję ilości zarodników grzyba Isaria fumosorosea zawartych w cieczy. Maksymalna redukcja zarodników wyniosła 89%

    Characterization and Identification of Isaria fumosorosea metabolite

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    The fungi in the genus Isaria produce a variety of inhibitory metabolites. Typically, Isaria are known as entomopathogenic fungi. However, we have isolated a strain of this fungus that produces a metabolite that inhibits the growth of other fungi. The metabolite was produced during growth in potato dextrose broth. The filtered broth was analyzed to quantify the antifungal activity of the metabolite. Using a panel of fungal plant pathogens, we determined the minimal inhibitory concentration (MIC) of the Isaria metabolite with XTT assay. The fungal spores of the test organisms were grown with and without the Isaria metabolite. The MIC of the metabolite was then compared to other known antifungal agents (ex. boric acid). Depending on the species tested, the metabolite demonstrated a higher or lower MIC in comparison to boric acid. TLC analysis was also done in order to identify how many (or if any) chemical components were made up of the metabolite in order to determine whether or not the metabolite was a protein or a molecule. Current studies are focused on characterizing the metabolite by examining the effects of temperature, pH, and light exposure on activity. Future studies will be concentrated on continuing to isolate and identify the metabolite.SUNY BrockportSUNY Undergraduate Research Conferenc

    Vyhodnocení variabilní aplikace hnojiv senzorovým systémem Isaria

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    This diploma thesis concerns the evaluation of a four-year sensor testing of the Fritzmeier ISARIA system. In a literature review is described nitrogen variable fertili-sation, the diagnostics of nutritious plant condition. The sensor system ISARIA is also described including vegetative index and individual modes settings. In a practical part is described four years testing of the Fritzmeier ISARIA system in a company Salix Morava a. s. in Zdounky region. In results there are listed parameters like yield, the quantity of applicated nitrogen, the effectivity of nitrogen application the quality of production and revenues. The results shows significant economic and environmental effects resulting from variable nitrogen application. The results are also statistically evaluated for enabling to be a part of a conclusion. It can be seen form the results, that a significant, impact for an economic contribution of a variable nitrogen application is affected by a field, year, cultivar, but also by settings of a sensor system
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