1,720,956 research outputs found
Functional characterisation of SLC2A3 and PFKP in t(8;21)-positive acute myeloid leukemia
Get PDFThe translocation t(8;21) is the most prevalent chromosomal rearrangement in acute
myeloid leukaemia (AML) and results in the expression of the oncogenic fusion
protein RUNX1/ETO. The fusion protein is required for maintaining the leukaemic
phenotype. Previous in vivo work identified genes related to glycolysis, namely
SLC2A3 and PFKP as potential transcriptional targets for RUNX1/ETO.
This project aimed to functionally investigate the role of SLC2A3 and PFKP in
RUNX1/ETO-driven leukaemia both in vivo and in vitro.
SLC2A3 significantly maintained the fitness of leukaemic cells under limited glucose
and hypoxic conditions in the RUNX1/ETO-expressing Kasumi-1 cell line. Notably,
SLC2A3 knockdown impaired the competitive fitness of patient-derived xenograft
(PDX) cells in high glucose and oxygen condition. Furthermore, PFKP knockdown
results in loss of populations both in vitro and in vivo. Besides, double knockdown of
SLC2A3 and PFKP caused G1 cell cycle arrest and had a greater effect in impairing
leukaemic cell propagation and fitness when compared to the single knockdowns.
Moreover, RUNX1/ETO-expressing cells showed to have a high sensitivity towards 2-
deoxyglucose (2DG). 2DG is an analogue of glucose molecule that blocks glycolysis
upstream of PFKP. RUNX1/ETO cells treated with this inhibitor undergo apoptosis
associated with the overall decrease in cell number. The combination of 2DG and cell
cycle inhibitor, palbociclib led to an additive effect and severely impaired expressions
of genes in the glycolysis pathway.
Collectively, the oncogenic fusion protein, RUNX1/ETO shows dependency on
glycolysis through SLC2A3 and PFKP. Targeting these two genes may instruct new
treatment strategies with reduced toxicity in t(8;21) leukaemia
Molecular Cytogenetics Of Myelodysplastic Syndrome – Hospital Based Study
Get PDFMyelodysplastic Syndrome (MDS) is a haematological condition characterised by hypercellular bone marrow and low peripheral blood counts. The objectives of this study were to identify chromosomal aberration using conventional cytogenetics and array comparative genomic hybridisation (array-CGH) in MDS patients and correlate with its’ haematological findings. A total of 8 patients were recruited. Cytogenetic mresults revealed six normal karyotypes, a karyotype with structural abnormalities
(del(5)(q13q33) and a karyotype with complex rearrangement (t(1;11), t(2;11), del(4p), del(5p), del(9p)). By array-CGH technique, known cytogenetic aberrations, complex chromosome aberrations and rare/cryptic aberrations (<5 Mb) were detected
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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