168,875 research outputs found
Mood disorders in familial epilepsy: A test of shared etiology
No full textObjective: Mood disorders are the most common comorbid conditions in epilepsy but the cause remains unclear. One possible explanation is a shared genetic susceptibility to epilepsy and mood disorders. We tested this hypothesis by evaluating lifetime prevalence of mood disorders in relatives with and without epilepsy in families containing multiple individuals with epilepsy, and comparing the findings with rates from a general population sample.
Methods: The Composite International Diagnostic Interview was administered to 192 individuals from 60 families, including 110 participants with epilepsy of unknown cause (50 focal epilepsy [FE], 42 generalized epilepsy [GE], 6 FE and GE, 12 unclassifiable) and 82 relatives without epilepsy (RWOE). Odds ratios (ORs) for lifetime prevalence of mood disorders in participants with versus without epilepsy were computed through logistic regression, using generalized estimation equations to account for familial clustering. Standardized prevalence ratios (SPRs) were used to compare prevalence in family members with general population rates.
Results: Compared with RWOE, ORs for mood disorders were significantly increased in participants with FE (OR = 2.4, 95% confidence interval [CI] = 1.1 - 5.2) but not in those with GE (OR = 1.0, 95% CI = 0.4 - 2.2). In addition, prevalence of mood disorders was increased in individuals with epilepsy who had ≥1 relative with FE. Compared with general population rates, mood disorders were significantly increased in individuals with FE but not in those with GE. Rates were also increased in RWOE, but not significantly so (SPR = 1.4, P = 0.14).
Significance: These findings are consistent with the hypothesis of shared genetic susceptibility to epilepsy and mood disorders, but suggest (1) the effect may be restricted to FE, and (2) the shared genetic effect on risk of mood disorders and epilepsy may be restricted to individuals with epilepsy, that is, to those in whom the genetic risk for epilepsy is “penetrant.”Peer reviewedThis is the accepted version of the following article: Insel BJ, Ottman R, Heiman GA. Mood disorders in familial epilepsy: A test of shared etiology. Epilepsia. 2018;00:1–9. https://doi.org/10.1111/epi.13985, which has been published in the Early View form at http://onlinelibrary.wiley.com/doi/10.1111/epi.13985/full. This article may be used for non-commercial purposes in accordance with the Wiley Self-Archiving Policy [https://authorservices.wiley.com/author-resources/Journal-Authors/licensing-open-access/open-access/self-archiving.html]
Role of extracellular signal-regulated kinase and PKC, in cytosolic PLA<sub>2</sub> activation by bradykinin in MDCK-D<sub>1</sub> cells
No full text Pages C1360–C1387: M. Xing, L. Tao, and P. A. Insel. “Role of extracellular signal-regulated kinase and PKC, in cytosolic PLA2 activation by bradykinin in MDCK-D1 cells.” Page C1380, line 27 from the top of the Abstract, there is a typographical error: the middle initial of P. A. Insel in the cited reference (C. Godson et al.) should be “A” not “S” and should read “C. Godson, K. S. Bell, and P. A. Insel. J. Biol. Chem. 268: 11946–11950, 1993.” </jats:p
DESENSITIZATION AND REDISTRIBUTION OF BETA-ADRENERGIC RECEPTORS ON HUMAN MONONUCLEAR LEUKOCYTES
No full textWe have used intact human mononuclear leukocytes (MNL) to examine desensitization of beta-adrenergic receptors in normal mammalian cells. MNL were prepared and radioligand binding experiments were performed at 4 degrees C. At this temperature the ligand [125I]iodocyanopindolol ([125I]ICYP) identified the same number of receptors as at 37 degrees C, and the agonist isoproterenol competed for this binding with high affinity (dissociation constant, Ki = 20 nM). At 37 degrees C, results were similar when the binding incubation was terminated after 1 min, but the apparent affinity of the receptors for isoproterenol was several 100-fold lower when the incubation was allowed to reach steady state. In desensitized MNL (prepared by incubating whole blood with 10 microM isoproterenol at 37 degrees C for 10 min, and then isolating and washing the MNL at 4 degrees C), isoproterenol-stimulated cAMP accumulation was reduced 63 +/- 4%. After desensitization, the total number of beta-receptors was unchanged, but isoproterenol and the hydrophilic antagonist CGP-12177 were able to compete with [125I]ICYP for binding to only 18 +/- 6% of these sites. Direct binding with [3H]CGP-12177 yielded similar results. These results demonstrate that isoproterenol promotes a rapid desensitization of beta-adrenergic receptors on MNL and a concomitant redistribution of receptors into a cellular compartment to which some ligands (including catecholamines) have restricted access. The findings demonstrate that redistribution of beta-receptors may be a mechanism mediating desensitization to catecholamines in normal mammalian cells
Klosterleben im Mittelalter nach zeitgenössischen Quellen hrsg. von Georg A. Narciss (Frankfurt am Main : Insel Verlag, 1988 ; in-8°, 622 pages, 38 planches [Insel Taschenbuch, 1135]).
No full textKlosterleben im Mittelalter nach zeitgenössischen Quellen hrsg. von Georg A. Narciss (Frankfurt am Main : Insel Verlag, 1988 ; in-8°, 622 pages, 38 planches [Insel Taschenbuch, 1135]).. In: Bibliothèque de l'école des chartes. 1990, tome 148, livraison 1. p. 227
Klosterleben im Mittelalter nach zeitgenössischen Quellen hrsg. von Georg A. Narciss (Frankfurt am Main : Insel Verlag, 1988 ; in-8°, 622 pages, 38 planches [Insel Taschenbuch, 1135]).
No full textKlosterleben im Mittelalter nach zeitgenössischen Quellen hrsg. von Georg A. Narciss (Frankfurt am Main : Insel Verlag, 1988 ; in-8°, 622 pages, 38 planches [Insel Taschenbuch, 1135]).. In: Bibliothèque de l'école des chartes. 1990, tome 148, livraison 1. p. 227
Changes in the fledging success over time with increasing population size in the Northern Lapwing Vanellus vanellus on Wangerooge Island (Lower Saxony, Germany)
No full textIn this study, we report the results of a long-term investigation on changes in population size and fledging success of Northern Lapwing on Wangerooge, a German Wadden Sea island. This population is increasing over a period of 34 years in contrast to numerous populations in North-western Europe. The reproductive success however declines over time and also with population density. Both effects cannot be considered separately due to autocorrelation. However, it is noted that the population on Wangerooge is not sustained by local recruitment only. This outcome is even more alarming as coastal areas and islands are considered as rare high quality meadow bird habitats. According to the present results Wangerooge cannot be considered as a source habitat for Northern Lapwings in North-western Germany.Die vorliegende Langzeitstudie beschreibt die Bestandsentwicklung und den Reproduktionserfolg des Kiebitzes (Vanellus vanellus) auf der Nordseeinsel Wangerooge (Niedersachsen, Deutschland). In den vergangenen 34 Jahren hat der Kiebitzbestand hier kontinuierlich zugenommen. Diese Entwicklung unterscheidet sich damit deutlich von denen anderer Kiebitzpopulationen in Nord- und Westeuropa. Der Bruterfolg des Kiebitzes auf Wangerooge nahm im gleichen Zeitraum mehr und mehr ab. Er zeigte zudem einen negativen Trend mit steigender Populationsgröße. Leider können beide Effekte aufgrund von Autokorrelation statistisch nicht separiert werden. Der mittlere Bruterfolg ist allerdings keineswegs hoch genug, um die Population selbst zu tragen. Dies ist alarmierend, da die Nordseeinseln und angrenzende Küstengebiete als qualitativ hochwertige Wiesenvogellebensräume gelten. Da nach derzeitigem Kenntnisstand der Bruterfolg des Kiebitzes auf Wangerooge nicht bestandserhaltend ist, kann die Insel auch nicht als „source-Habitat“ für diese Limikolenart gelten
Agonist-induced redistribution of beta-adrenergic receptors on intact human mononuclear leukocytes: redistributed receptors are nonfunctional.
No full textIncubation of human mononuclear leukocytes (MLN) with isoproterenol rapidly desensitizes beta-adrenergic receptors, i.e. isoproterenol-stimulated cAMP accumulation decreases. This desensitization is accompanied by a redistribution of the receptor into a cellular environment to which hydrophilic compounds have limited access. We found that the total number of beta-receptors [defined as binding of [3H]dihydroalprenolol (DHA) inhibited by 1 microM propranolol] was unchanged in the desensitized MNL. In control MNL, virtually all DHA binding was inhibited by 1 microM CGP-12177, suggesting that all of these receptors are on the cell surface, whereas in desensitized cells, only 33 +/- 2% (mean +/- SEM) of the DHA binding was inhibited by CGP-12177. We quantitated the sequestered receptors by subtracting the number of surface receptors from the total number of receptors. The sequestered receptors were homogeneous, with an affinity for DHA identical to that of surface receptors (Kd, 0.66 +/- 0.12 vs. 0.62 +/- 0.08 nM). The time courses of desensitization and sequestration were identical. The functional status of the sequestered receptors was assessed using the agonist zinterol, which (unlike catecholamines) is quite hydrophobic. Zinterol competed for DHA binding to both sequestered and surface receptors, whereas isoproterenol only competed for binding to the surface receptors. However, cAMP accumulation in desensitized MNL was reduced to the same extent regardless of whether isoproterenol or zinterol was used as the agonist. These results demonstrate that desensitization of intact cells to beta-agonists cannot be attributed to limited accessibility of the sequestered receptors to catecholamines, but, rather, that the sequestered receptors are not functionally coupled to adenylate cyclase
A 2 h periodic variation in the low-mass X-ray binary Ser X-1
No full textSpectroscopy of the low-mass X-ray binary Ser X-1 using the Gran Telescopio Canarias have revealed a ?2 h periodic variability that is present in the three strongest emission lines. We tentatively interpret this variability as due to orbital motion, making it the first indication of the orbital period of Ser X-1. Together with the fact that the emission lines are remarkably narrow, but still resolved, we show that a main-sequence K dwarf together with a canonical 1.4 M? neutron star gives a good description of the system. In this scenario, the most likely place for the emission lines to arise is the accretion disc, instead of a localized region in the binary (such as the irradiated surface or the stream-impact point), and their narrowness is due instead to the low inclination (?10°) of Ser X-1
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Dr. Edward P. Wimberly, ITC, July 2011
No full textThis video is a conversation with Dr. Edward P. Wimberly. Dr. Wimberly talks about his book, "No Shame in Wesley's Gospel: A Twenty-First Century Pastoral Gospel". Brad Ost, AUC Woodruff Library, is the interviewer
- …
