17 research outputs found
Monitoring middleware for distributed applications
No full textWith growing maturity Internet services are proving integral to the provision of computer services. To provide consistent end-user experiences these services are increasingly augmented with some notion of 'Quality-of-Service' (QoS), which typically requires the management of computing resources to maintain a predictable level of service performance. It is difficult to guarantee consistent servIce provision In dynamic and open environments such as the Internet. However service monitoring can be used to inform compensatory actions by collecting meaningful service performance data from strategic points in an active service environment. Due to the unpredictable nature of the Internet distributed monitoring mechanisms face challenges with respect to the various communication protocols, application languages, and monitoring requirements associated with a service environment. With the growing popularity of Internet services creation of monitoring solutions on a per- service basis becomes time-consuming and misses opportunities to re-use existing logic. Ideally monitoring solutions would be domain-agnostic, automatically generated and automatically deployed. This thesis progresses these ambitions by providing a generic, distributed monitoring and evaluation framework based on Metric Collector (MeCo) components. These components can transparently gather measurement data across a range of service technologies as used within E-Commerce service environments. MeCo components form part of a framework which can interpret Service Level Agreements (SLAs) to automatically provide tailored service monitoring. The evaluation paradigms of the Meeo Framework are re-appropriated for use in Distributed Virtual Environments (DYEs). Quantifiable QoS requirements are established for Interest Management mechanisms (which limit message production based on object localities within a DYE). These are then incorporated into a DVE Simulator application. This application allows DYE application developers to evaluate Interest Management configurations for their suitability. Extensions to the DVE Simulator are exhibited in the Evolutionary Optimisation Simulator (EOS), which provides automated optimisation capabilities for DVE configurations through utilisation of genetic algorithm techniques.EThOS - Electronic Theses Online ServiceGBUnited Kingdo
Metallo-dielectric metasurfaces for thermal emission with controlled spectral bandwidth and angular aperture
No full textInternational audienceWe introduce thermal metallo-dielectric metasurfaces as mid IR sources. The emitter is a lossy metal. The spectral and angular emission is controlled using a periodic array of high refractive dielectric resonators. We introduce a design that allows to control independently the emission bandwidth and the angular aperture while ensuring a large emissivity. To validate the concept, we fabricated and characterized a metasurface, showing a good agreement with the theory
Combined influence of insulin resistance, overweight/obesity and fatty liver as risk factors for type 2 diabetes
No full textOBJECTIVE There is dissociation between insulin resistance, overweight/obesity, and fatty liver as risk factors for type 2 diabetes, suggesting that different mechanisms are involved. Our aim was to 1) quantify risk of incident diabetes at follow-up with different combinations of these risk factors at baseline and 2) determine whether each is an independent risk factor for diabetes.RESEARCH DESIGN AND METHODS We examined 12,853 subjects without diabetes from a South Korean occupational cohort, and insulin resistance (IR) (homeostasis model assessment-IR ?75th centile, ?2.0), fatty liver (defined by standard ultrasound criteria), and overweight/obesity (BMI ?25 kg/m2) identified at baseline. Odds ratios (ORs) and 95% confidence intervals (CIs) for incident diabetes at 5-year follow-up were estimated using logistic regression.RESULTS We identified 223 incident cases of diabetes from which 26 subjects had none of the three risk factors, 37 had one, 56 had two, and 104 had three. In the fully adjusted model, the OR and CI for diabetes were 3.92 (2.86–5.37) for IR, 1.62 (1.17–2.24) for overweight/obesity, and 2.42 (1.74–3.36) for fatty liver. The OR for the presence of all three factors in a fully adjusted model was 14.13 [8.99–22.21].CONCLUSIONS The clustering of IR, overweight/obesity, and fatty liver is common and markedly increases the odds of developing type 2 diabetes, but these factors also have effects independently of each other and of confounding factors. The data suggest that treatment for each factor is needed to decrease risk of type 2 diabetes
Exosomes-Derived MiR-302b Suppresses Lung Cancer Cell Proliferation and Migration via TGFβRII Inhibition
No full textBackground/Aims: Several studies have reported that tumor-derived exosomes contain kinds of miRNAs, including oncogenic miRNAs and tumor suppressor miRNAs. It has been reported that miR-302b could inhibit cancer progression by targeting oncogenes post-transcriptionally. Whether miR-302b is involved in the regulation of tumor-derived exosomes on lung cancer cells proliferation has not yet been demonstrated. This study aimed to examine the effect of exosomes-derived miR-302b on lung cancer cells proliferation and explain the potential mechanism. Methods: The effect of exosomes derived from 95C cells and 95D cells with different metastatic ability on lung cancer cell proliferation and migration were analyzed by MTT assay and Transwell assays, respectively. Exosomes of 95C and 95D cells derived miR-302b was quantified by qRT-PCR, the effect of miR-302b on proliferation and migration of 95D cells was analyzed by MTT assays and Transwell assays respectively after transfection with miR-302b, while the expression of phosphorylated ERK1/2, MMP9 and TGFβRⅡ was analyzed by Western blot. The target gene of miRNA-302b was analyzed by Luciferase reporter assays. Results: 95C-derived exosomes significantly decreased the migration ability of 95D cells. miRNA-302b was significantly overexpressed in 95C cells and 95C-derived exosomes compared with 95D cells and 95D-derived exosomes. Transfection of miR-302b into 95D cells significantly suppressed cells proliferation and migration capabilities along with the down-regulated expression of TGFβRII, phosphorylated ERK1/2 and MMP9 induced by TGF-β1. Conclusions: Exosomes-derived miR-302b could suppress lung cancer cell proliferation and migration via the TGFβRII/ERK pathway and thus provides a potential target for human lung cancer therapy
Construyendo una lupa para mirar la división social del espacio en la ciudad de Buenos Aires: la segregación residencial revisitada en diferentes escalas
No full textLa segregación residencial es una característica de la metrópolis capitalista y, por ende, de las ciudades de América
Latina. Dos han sido los modos en los que la literatura académica en la región ha avanzado en el abordaje de estas
cuestiones. Por un lado, se ha enfocado al análisis de los patrones de segregación propios de las áreas
metropolitanas latinoamericanas. Por el otro, la literatura también se ha orientado a la indagación de la vida
cotidiana en los entornos segregados. De este modo, si bien existe en la región un conocimiento considerable sobre
distintos aspectos de los procesos de segregación residencial, poco se ha indagado aún en las cuestiones vinculadas
a los efectos de las escalas socio-territoriales en la indagación de tales procesos. En este marco, el trabajo que aquí se presenta
intenta recuperar la importancia de las escalas socio-territoriales para comprender la composición y características de los
entornos segregados en las metrópolis latinoamericanas, en general, y en la Ciudad de Buenos Aires, en particular.
Con base en resultados de una investigación que indaga las transformaciones ocurridas en las antiguas periferias de
Buenos Aires, en general, y en los diferentes tipos de hábitats que allí se localizan, en particular, el artículo pasa
revista a la importancia de la escala en el abordaje de la segregación residencial e indaga cómo el cambio de escala,
interpela a los factores que intervienen en la definición del patrón de segregación a gran escala y da cuenta de cómo
dichos factores operan a escala micro
An Integrative Approach to the Assessment of the Coordination Abilities in Equestrian Athletes at the Initial Stage of Training
No full textРыженкова Татьяна Алексеевна, вице-президент, Общественная организация «Самарская областная федерация конного спорта». 443111, г. Самара, Московское шоссе, 125 Б. E-mail: [email protected], ORCID: 0000-0002-8755-6321.
Горелик Виктор Владимирович, кандидат биологических наук, доцент кафедры адаптивной физической культуры, спорта и туризма, Тольяттинский государственный университет. 445020, Самарская область, г. Тольятти, ул. Белорусская, 14. E-mail: [email protected], ORCID: 0000-0001-8767-5200.
Хайрова Зульфия Рафиковна, аспирант кафедры социальной и дифференциальной психологии, Российский университет дружбы народов. 117198, г. Москва, ул. Миклухо-Маклая, 6. E-mail: [email protected], ORCID: 0000-0002-5367-2156.
Кнышева Татьяна Петровна, психолог, Центр интеллектуального развития «Эврика». 445031, г. Тольятти, ул. 70 лет Октября, 48. E-mail: [email protected], ORCID: 0000-0002-1518-3490.
T.A. Ryzhenkova1, [email protected], ORCID: 0000-0002-8755-6321,
V.V. Gorelik2, [email protected], ORCID: 0000-0001-8767-5200,
Z.R. Khairova3, [email protected], ORCID: 0000-0002-5367-2156,
T.P. Knysheva4, [email protected], ORCID: 0000-0002-1518-3490
1Public Organization Samara Regional Federation of Equestrian Sports, Togliatti, Russian Federation,
2Togliatti State University, Togliatti, Russian Federation,
3Peoples Friendship University of Russia, Moscow, Russian Federation,
4Center for Intellectual Development “Eureka”, Tolyatti, Russian FederationЦель исследования. Развитие координационных способностей у спортсменов-конников начального этапа подготовки с помощью специально организованного учебно-тренировочного процесса. Материалы и методы исследования. Исследование проводилось на базе спортивного оздоровительного комплекса «Гран-при» с. Подстепки Самарской области. Обследовано 36 спортсменов-конников в возрасте от 16 до 17 лет. Основную группу (ОГ) и группу сравнения (ГС) составили юноши и девушки по 18 человек. Участники ГС занимались по стандартной программе 4 дня в неделю по 1 часу, спортсмены ОГ тренировались
по 4 дня в неделю, в четвертый день в ОГ применялась методика специально подобранных упражнений, направленных на развитие координационных способностей. Методика диагностики включала компьютерную программу «Исследователь временных и пространственных свойств человека версия 2.1», тренировочную платформу BALANCE SYSTEM SD. Результаты. По результатам исследования значительные изменения в сторону улучшения показателей при выполнении всех тестов оценки пространственновременных свойств отмечаются у троеборцев и конкуристов как у юношей, так и у девушек. Результаты достоверности различий односторонней и двусторонней постуральной
стабильности подтверждают значимость способности к статическому и динамическому равновесию, балансу, координированию и комбинированию движений для спортсменовконников, занимающихся конкуром, выездкой и троеборьем. Заключение. Использование специальных упражнений на стабилоплатформе позволяет улучшить: координационные способности, пространственно-временные свойства и показатели постуральной стабильности в разных видах конного спорта «конкур», «выездка», «троеборье» в ОГ. Aim. The article deals with developing coordination abilities in equestrian athletes at the initial
stage of training through a specially organized training process. Materials and methods.
The study was conducted on the premises of the Grand Prix sports and recreation complex (Samara region, Podstepki village, Togliatti). 36 equestrian athletes aged from 16 to 17 years were examined. The main (MG) and the comparison group (CG) consisted of 18 males and females. The comparison group was engaged in a standard program (4 days per week, 1 hour). The main group had 4 training sessions per week. On the fourth day, the main group practiced specially selected exercises aimed at developing coordination abilities. The diagnostic method included the Human temporal and spatial properties analyzer 2.1 software, as well as the BALANCE SYSTEM SD training platform. Results. According to the results of the study, significant changes in the spatial and temporal properties were observed among triathletes and competitors
in both males and females. The significance of differences of unilateral and bilateral postural stability confirms the importance of the ability to static and dynamic balance, as well as coordination and combination of movements for equestrian athletes involved in show jumping, dressage and eventing. Conclusion. Special exercises on the force platform allow improving coordination abilities, temporal and spatial properties and postural stability in show jumping, dressage and eventing
The lack of WIP reduces B cell amoeboid motility.
No full text<p><b>a</b> Chemotactic response of freshly isolated WIP<sup>+/+</sup> and WIP<sup>−/−</sup> B cells to CXCL13 (400 nM) in Boyden chambers. <b>b</b> Polarization and migration frequencies of WIP<sup>+/+</sup> and WIP<sup>−/−</sup> B cells settled on ICAM-1-containing planar membranes coated with CXCL13. In (<b>a</b>) and (<b>b</b>) each dot corresponds to one experiment; black thick bar, averaged value. <b>c</b> Mean speed values (left panel) and Straightness index (right panel) of WIP<sup>+/+</sup> and WIP<sup>−/−</sup> B cells in the same conditions than in (<b>b</b>); each dot corresponds to a single cell. <b>d</b> Tracks of representative WIP<sup>+/+</sup> and WIP<sup>−/−</sup> B cells migrating on the planar membranes; each line corresponds to a single cell track. <b>e</b> DIC and F-actin images of representative WIP<sup>+/+</sup> (left panels) and WIP<sup>−/−</sup> (middle panel) B cells; right panel, lamellipodium frequency in control and deficient B cells. Data on (<b>c</b>) and (<b>e</b>) is the merge of three experiments. *, p<0.05; **, p<0.001; ***, p<0.0001.</p
Nck binding to WIP contributes to chemotaxis towards PDGF-AA.
No full text<p>WIP<sup>−/−</sup> murine fibroblasts were transduced with recombinant lentivirus engineered to express control GFP or fusion constructs including WIP, WIPΔNBD or WIPΔWBD. Fibroblasts were loaded onto the upper chamber of the Transwell and the percentage of GFP-expressing cells chemotacting towards 50 ng/ml PDGF-AA was calculated. * p<0,05.</p
WIP deficiency does not affect fibroblast morphology or protein content.
No full text<p><b>a</b> Representative western blot of WIP, N-WASP and Nck expression in soluble lysates of lung-derived fibroblasts from WIP<sup>+/+</sup> and WIP<sup>−/−</sup> mice. Numbers indicate relative expression levels of each protein to GAPDH content and control fibroblasts determined by densitometry. GAPDH labeling confirmed equivalent protein loading control. <b>b</b> FACS analysis of forward and side scatter in control (WIP<sup>+/+</sup>) and WIP<sup>−/−</sup> fibroblasts. <b>c</b> Phase-contrast images from plated control (WIP<sup>+/+</sup>) and WIP<sup>−/−</sup> murine fibroblasts do not show evident morphological differences between both populations. Scale bar 50 µm.</p
PDGF-AA-induced dorsal ruffle formation is diminished in WIP <sup>−/−</sup> fibroblasts.
No full text<p><b>a</b> Control (WIP<sup>+/+</sup>) and WIP<sup>−/−</sup> primary murine fibroblasts were serum starved over night (0 min) or serum starved and stimulated with PDGF-AA for increasing times (8 and 15 min). Fixed and permeabilised cells were stained with anti-cortactin and FITC-secondary antibody and imaged in a Zeiss microscope to identify dorsal ruffles (white arrow). Magnifications of the boxed areas are shown as right panels. <b>b</b> The percentage of WIP<sup>+/+</sup>(black) and WIP<sup>−/−</sup> (white) cells forming dorsal ruffles after PDGF-AA stimulation is plotted against incubation times. <b>c</b> WIP<sup>−/−</sup> primary fibroblasts were lentivirally transduced to express control cherry or WIP-cherry, starved and incubated with PDGF-AA for 8 or 15 min. Fixed cells were imaged. <b>d</b> The percentage of WIP<sup>−/−</sup> cells expressing cherry (white) or WIP-cherry (black) and forming dorsal ruffles after PDGF-AA stimulation is plotted against incubation times. <b>e</b> Representative western blot of PDGFRα expression in soluble lysates of lung-derived fibroblasts from WIP<sup>+/+</sup> and WIP<sup>−/−</sup> mice. Numbers indicate relative expression levels of the protein to control fibroblasts determined by densitometry. ß-actin labeling confirmed equivalent protein loading control. <b>f</b> WIP<sup>+/+</sup> (black) and WIP<sup>−/−</sup> (white) primary fibroblasts were grown in the presence of serum (left panel) and stained with anti-PDGFRα plus labeled secondary antibody and analysed by FACS. The mean fluorescence intensity of positive cells in both populations is represented. Right panel, WIP<sup>+/+</sup> (black) and WIP<sup>−/−</sup> (white) primary fibroblasts were starved (time 0) and stimulated with PDGF-AA and stained as above. Percent of mean fluorescence intensity relative to starvation is represented. Arbitrary units (a.u.). * p<0,05.</p
