1,720,967 research outputs found
Variazioni reciproche nella concentrazione degli RNAm della ornitina decarbossilasi e dell'SGP-2: possibile ruolo nel controllo dell'omeostasi cellulare
No full textStudies on the relationship between cell proliferation and cell death: apposite patterns of SGP-2 and ornithine decarboxylase mRNA accumulation in PHA-stimulated human limphocytes
No full textRegional and cellular distribution within the rat prostate of two mRNA species undergoing opposite regulation by androgens
No full textRegional increases in ornithine decarboxylase mRNA levels in the rat brain after partial mesodiencephalic hemitransection as revealed by in situ hybridization histochemistry
No full textSTUDIES ON THE RELATIONSHIP BETWEEN CELL-PROLIFERATION AND CELL-DEATH - OPPOSITE PATTERNS OF SGP-2 AND ORNITHINE DECARBOXYLASE MESSENGER-RNA ACCUMULATION IN PHA-STIMULATED HUMAN-LYMPHOCYTES
No full textTo assess the relationship between cell proliferation and cell death, the mRNA accumulation of ornithine decarboxylase (ODC)and sulfated glycoprotein 2 (SGP-2) were measured in human peripheral blood lymphocytes (HPBL), 2-6 hours after stimulation with phytohemoagglutinin (PHA). A consistent amount of SGP-2 mRNA in quiescent HPBL, an early and progressive decrease of SGP-2 mRNA and a parallel increase of ODC mRNA accumulation, were observed, in PHA-stimulated HPBL, suggesting that concomitant repression of SGP-2 and induction of ODC genes contribute for the cell entering the cell cycle
REGIONAL AND CELLULAR-DISTRIBUTION WITHIN THE RAT PROSTATE OF 2 MESSENGER-RNA SPECIES UNDERGOING OPPOSITE REGULATION BY ANDROGENS
No full textWe have investigated, by in-situ hybridization histochemistry, the distribution within the rat ventral prostate of the mRNAs for sulphated glycoprotein-2 (SGP-2) and ornithine decarboxylase, two proteins that appear to be inversely regulated by androgens in this organ, in that the level of SGP-2 mRNA is lowered, while the activity of ODC is enhanced by the latter hormones. Low-magnification autoradiograms of whole ventral prostate sections showed that, in intact animals, the SGP-2 transcript was only detectable in restricted areas and not diffused evenly throughout the section. Reciprocally, the ODC transcript was not detectable in areas where the SGP-2 transcript was detected, but appeared distributed uniformly in the remaining parts of the section. The effect of castration on the levels of the two mRNAs was evaluated by a semiquantitative analysis of autoradiograms from whole ventral prostate sections using an autoimmune image analyser. Four days after castration, SGP-2 mRNA increased by about 11-fold, while ODC mRNA decreased by fivefold. The distribution of the two mRNAs among the different cell types, studied by treating the slides with photographic emulsion and counterstaining, showed that both were expressed exclusively in the epithelial luminal cells of the ducts. Furthermore, each of the two mRNAs preferentially accumulated in a cell population which was morphologically distinct while accumulation of the other was negligible. SGP-2 mRNA was mostly found in cuboidal epithelial cells and ODC mRNA in columnar epithelial cells. Castration caused a dramatic accumulation of SGP-2 and a decrease in ODC mRNAs in the cells of the columnar epithelium 4 days later. These data suggest that, in the ventral prostate of intact animals, SGP-2 is expressed in the proximal ducts whose luminal epithelium consists of cuboidal cells undergoing apoptotic phenomena and not in the intermediate and distal ducts characterized by columnar epithelia with active protein synthesis and cell multiplication. In the intermediate and distal parts of the ductal system the ODC gene would be expressed while being turned off in the proximal ducts. Castration, resulting in apoptosis of the epithelial cells of the intermediate and distal ducts, caused SGP-2 to be actively expressed and ODC to be represse
REGIONAL INCREASES IN ORNITHINE DECARBOXYLASE MESSENGER-RNA LEVELS IN THE RAT-BRAIN AFTER PARTIAL MESODIENCEPHALIC HEMITRANSECTION AS REVEALED BY INSITU HYBRIDIZATION HISTOCHEMISTRY
No full textChanges in the level of ornithine decarboxylase mRNA after partial mesodiencephalic hemitransection were evaluated in various regions of the rat brain by means of in situ hybridization histochemistry coupled with computer-assisted image analysis. On days 1 and 2 after the lesion, increased accumulation of ornithine decarboxylase mRNA was observed on the lesioned side in various telencephalic regions (e.g. neostriatum and frontoparietal cortex), and in the pars compacta of the substantia nigra. Both in the frontoparietal cortex and substantia nigra a decreasing gradient of ornithine decarboxylase mRNA activation was observed going far from the site of the lesion. Seven days after the operation, ornithine decarboxylase mRNA levels returned to control values on the lesioned side but increased in some regions, such as the frontoparietal cortex, on the intact side. The present results demonstrate that the parent cell body biosynthetic machinery is activated by the mechanical lesion of the axons at the level of ornithine decarboxylase gene expression. The increase of ornithine decarboxylase mRNA is not as large as the enhancement in ornithine decarboxylase activity previously shown, suggesting that the response to the lesion may also involve changes in the rate of translation of ornithine decarboxylase mRNA and/or in the rate of degradation of ornithine decarboxylase protein
In vivo accumulation of Sulfated Glycoprotein 2 (SGP-2) mRNA in rat thymocytes upon dexamethasone induced cell death
No full textINVIVO ACCUMULATION OF SULFATED GLYCOPROTEIN-2 MESSENGER-RNA IN RAT THYMOCYTES UPON DEXAMETHASONE-INDUCED CELL-DEATH
No full textTwo hours after a single intraperitoneal injection of dexamethasone into adult male rats, a typical ladder of DNA fragments was detectable upon separation on agarose gel of DNA from thymocytes. Accumulation of sulfated glycoprotein-2 mRNA, whose rate of expression has been associated to the processes of programmed cell death, precede the appearence of DNA degradation
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