200,871 research outputs found
Dendritic cell progenitors phagocytose particulates, including Bacillus Calmette-Guerin organisms, and sensitize mice to mycobacterial antigens in vivo
No full textInaba, K., Inaba, M., Naito, M., and Steinman, R.M. Dendritic cell progenitors phagocytose particulates, including Bacillus Calmette-Guerin organisms, and sensitize mice to mycobacterial antigens in vivo. J. Exp. Med. 178: 479-488, 1993https://digitalcommons.rockefeller.edu/historical-scientific-reports/1036/thumbnail.jp
The net reproduction rate and the type-reproduction number in multiregional demography
No full textIn order to study effects of migration on demographic changes of multiregional populations, multiregional population modelling is a useful traditional tool. Although multiregional mathematical demography has been extensively explored since the beginning of the 1970s, its key concept, the multiregional net reproduction rate, has been long neglected. In this review, we focus on a multiregional stable population system and elaborate the definition of the multiregional net reproduction rate. Next we introduce the type-reproduction number from mathematical epidemiology and show that it becomes a useful index to formulate a simple control relation for a multiregional population. Mathematical ideas presented here will help us to reconsider multiregional mathematical demography, which is a useful theoretical framework to study effects of interregional migration on population dynamics and composition.
The H current blocker ZD7288 decreases epileptiform hyperexcitability in the rat neocortex by depressing synaptic transmission
No full textNeurons respond to intracellular injection of hyperpolarizing current pulses by generating depolarizing sags contributed by a cation current termed I-h. I-h modulates neuron excitability and rhythmicity. It is, however, unclear whether the net effect of changing Ih leads to facilitation or depression of cortical epileptiform activity. Here, we addressed this issue by using field and intracellular recordings to study the effects of ZD7288 (10-100 mu M), a bradycardic agent known to abolish I-h, on the epileptiform discharges (duration = 2.5 +/- 0.3 s, mean +/- SEM; interval of occurrence = 34.2 +/- 3.3 s, n = 30 slices) induced in rat neocortical slices by 4-aminopyridine and GABA receptor antagonists. ZD7288 abolished the depolarizing sags seen during injection of intracellular hyperpolarizing current pulses while increasing resting membrane potential and apparent input resistance. These effects, which were fully established with 10 mu M ZD7288, were associated with a dose-dependent decrease in the occurrence of spontaneous epileptiform events and a reduction in their duration (the latter change occurring at doses > 20 mu M). ZD7288 also caused a dose-dependent decrease of background postsynaptic potentials. Finally, ZD7288 could depress epileptiform activity during Cs+ pretreatment, a procedure known to block Ih. These data indicate that ZD7288 hampers neocortical epileptiform synchronization, but also suggest that most of this action reflects the ability of ZD7288 to decrease synaptic transmission. (c) 2006 Elsevier Ltd. All rights reserved
Correlation of structure and function in the oxidative phosphorylation system of submitochondrial particles
No full text1. After the centrifugation of sonicated heavy beef heart mitochondria at 75, 000 × g for 10 minutes, the supernatant was centrifuged at 144, 000 × g for 30 minutes. The residue was revealed being composed of
vesicular inner membrane fragments (ETPH), about 600 to 1000 Å. in diameter, showing a morphological homogeneity and a high capacity of oxidative phosphorylation. 2. The Pia ratio of the ETPH in the presence of succinate and of NADH2 was 1.68 and 2.54, respectively, and the corrected Pia value for O2 gas equilibrium was 1. 01 and 1.40, respectively.
3. The capacity of oxidative phosphorylation in ETPH fraction was parallel to the activity of the oligomycin. sensitive ATPase in these fractions. 4. The P/0 ratio of ETPH was decreased to about 50 % by hypotonic
treatment. The decrease of P/0 ratio was restored to the level of about 90 % by incubating the ETPH with ATP and BSA. In the instance where the P/0 ratio was low level in the hypotonic medium, the surface structure of ETPH was observed as a swollen form and the head pieces
of the elementary particles were clearly observed in contrast to the solid surface structure of ETPH in the isotonic medium. 5. The P/0 ratio of ETPH was decreased to about 60 % by relatively severe sonication, and after separating the residue from the supernatant, that of the residue decreased further to about 40 %. The P/0 ratio of the residue was restored to the level before the separation on the addition of the supernatant containing oligomycin-insensitive ATPase. 6. A discussion was made on the correlation between the surface structure and the activities at terminal phosphorylation step of ETPH
after the simple physico-chemical treatment.</p
Correlation between fold increase of Inaba OSP specific antibody responses and cumulative diarrheal volume.
No full textX axis represents the fold-increase (day 10 to day 0) of IgM, IgA, and IgG responses against V. cholerae O1 Inaba OSP and Y axis represents cumulative diarrhea following experimental challenge with wild type V. cholerae O1 Inaba 10 or 90 days post vaccination with Inaba CVD 103-HgR. Dashed horizontal lines mark moderate (3L) or severe (5L) diarrheal values. Dashed vertical line denotes 1.5-fold anti-OSP antibody value change (day 10 post-vaccination compared to day 0 pre-vaccination).</p
O1 Inaba O-Specific Polysaccharide Bound to a Protein Carrier Are Immunogenic in Mice but Do Not Induce Protective Antibodies
No full textDevelopment of Vibrio cholerae lipopolysaccharide (LPS) as a cholera vaccine immunogen is justified by the correlation of vibriocidal anti-LPS response with immunity. Two V. cholerae O1 LPS serotypes, Inaba and Ogawa, are associated with endemic and pandemic cholera. Both serotypes induce protective antibody following infection or vaccination. Structurally, the LPSs that define the serotypes are identical except for the terminal perosamine moiety, which has a methoxyl group at position 2 in Ogawa but a hydroxyl group in Inaba. The terminal sugar of the Ogawa LPS is a protective B-cell epitope. We chemically synthesized the terminal hexasaccharides of V. cholerae serotype Ogawa, which comprises in part the O-specific polysaccharide component of the native LPS, and coupled the oligosaccharide at different molar ratios to bovine serum albumin (BSA). Our initial studies with Ogawa immunogens showed that the conjugates induced protective antibody. We hypothesized that antibodies specific for the terminal sugar of Inaba LPS would also be protective. Neoglycoconjugates were prepared from synthetic Inaba oligosaccharides (disaccharide, tetrasaccharide, and hexasaccharide) and BSA at different levels of substitution. BALB/c mice responded to the Inaba carbohydrate (CHO)-BSA conjugates with levels of serum antibodies of comparable magnitude to those of mice immunized with Ogawa CHO-BSA conjugates, but the Inaba-specific antibodies (immunoglobulin M [IgM] and IgG1) were neither vibriocidal nor protective in the infant mouse cholera model. We hypothesize that the anti-Inaba antibodies induced by the Inaba CHO-BSA conjugates have enough affinity to be screened via enzyme-linked immunosorbent assay but not enough to be protective in vivo
Serum Inaba OSP-specific antibody isotype fold increase (≥1.5) and Inaba-specific vibriocidal fold increase (≥4) on day 10 following vaccination as a predictor of protection against development of mild, moderate or severe diarrhea following subsequent challenge.
No full textSerum Inaba OSP-specific antibody isotype fold increase (≥1.5) and Inaba-specific vibriocidal fold increase (≥4) on day 10 following vaccination as a predictor of protection against development of mild, moderate or severe diarrhea following subsequent challenge.</p
Serum Inaba OSP-specific antibody isotype fold increase (≥1.5) and Inaba-specific vibriocidal fold increase (≥4) on day 10 following vaccination as a predictor of protection against development of moderate or severe diarrhea following subsequent challenge.
No full textSerum Inaba OSP-specific antibody isotype fold increase (≥1.5) and Inaba-specific vibriocidal fold increase (≥4) on day 10 following vaccination as a predictor of protection against development of moderate or severe diarrhea following subsequent challenge.</p
TINJAUAN PELAKSANAAN PELAYANAN JASA PADA BANK RAKYAT INDONESIA KK STIE INABA CABANG AHMAD YANI BANDUNG
No full textNama Moch. Bayu Raditya, NPM 04.05.113, Pembimbing Hj. Wien Dyahrini, S.E., M.S. judul skripsi Tinjauan Pelaksanaan Pelayanan Jasa Pada PT. Bank BRI (Persero) Tbk. Kantor Kas STIE INABA Cabang Ahmad Yani Bandung,
Perbankan merupakan salah satu bisnis yang bergerak dibidang jasa. Yang kegiatan atau aktivitas bisnisnya juga berkaitan dengan pelayanan. Pada dasarnya pelayanan bank terhadap nasabahnya hampir sama dan mudah ditiru oleh bank lainnya. Keunggulan bersaing dalam bisnis dapat dilihat dari kemapuan bank dalam proses pelaksanan pelayanan baik pratransaksi, pada saat transaksi, dan pascatransaksi. Atas dasar inilah penulis tertarik menyusun Laporan Tugas Akhir ini dengan judul “TINJAUAN PELAKSANAAN PELAYANAN JASA PADA BANK BRI (PERSERO) TBK. KANTOR KAS STIE INABA CABANG AHMAD YANI BANDUNG.”
Penelitian ini bertujuan untuk mengetahui Pelaksanaan pelayanan nasabah, masalah-masalah yang dihadapi dalam pelaksanaan pelayanan jasa, dan solusi untuk mengatasi masalah yang timbul dalam pelaksanaan pelayanana nasabah pada Bank BRI (Persero) Tbk. Kantor Kas STIE INABA Cabang Ahmad Yani Bandung. Metode yang digunakan dalam penelitian ini adalah metode deskriptif yaitu yang bertujuan untuk mengumpulkan data yang diperoleh selama penelitian kemudian diproses dan dianalisis dengan menggunakan dasar-dasar teori yang diteliti. Adapun teknik pengumpulan data yang digunakan adalah Wawancara (Interview), Observasi, dan studi kepustakaan.
Dari hasil penelitian, diperoleh kesimpulan bahwa dalam proses pelaksanaan pelayanan nasabah yang dilakukan Bank BRI (Persero) Tbk. Kantor Kas STIE INABA Cabang Ahmad Yani Bandung. bandung terdapat beberapa tahapan dari nasabah datang sampai keperluan mereka terpenuhi. Terdapat beberapa masalah yang timbul salam proses pelayanan nasabah diantaranya Sistem pelayanan yang kurang baik, Kurangnaya Sumber Daya Manusia yang dipekerjakan, Keterbatasan persedian uang tunai, dan Gangguan teknis
Epileptiform hyperexcitability in the rat neocortex: modulation by the H current blocker ZD7288
No full textObjectives: Neurons in the CNS respond to intracellular injection of hyperpolarizing current pulses by generating depolarizing sags contributed by a cation current termed Ih. Ih modulates neuron excitability and rhythmicity. However, whether the net effect of Ih on cortical networks results in facilitation or depression of epileptiform activity remains debatable. Here, we addressed this issue by studying the effects of the Ih blocker ZD7288 on the epileptiform discharges.Methods: We studied with field and intracellular recordings the effects of the Ih blocker ZD7288 on the epileptiform discharges (duration=2.54±0.33s, mean±SEM; interval of occurrence=34.2±3.3s, n=30) induced in rat neocortical slices by bath applying 4- aminopyridine+picrotoxin+CGP55845.Results: ZD7288 (10–100μM; n=18) abolished the depolarizing sags seen during injection of intracellular hyperpolarizing current pulses while increasing both resting membrane potential and apparent input resistance. These effects were fully established with 10μM ZD7288 and were accompanied by a dosedependent decrease in the occurrence of spontaneous epileptiform events and a reduction in their duration (this last effect becoming apparent with concentrations >20μM). ZD7288 (10–100μM; n=17) also caused a dose-dependent decrease of background postsynaptic potentials. Finally, 10, 50 or 100μM ZD7288 (n=6, 5 and 8, respectively) depressed the epileptiform activity during application of Cs+, which is known to reduce Ih.Conclusion: This evidence indicates that ZD7288 depresses neocortical epileptiform synchronization. However, most of this action may reflect the ability of ZD7288 to interfer with synaptic transmission
- …
