1,721,391 research outputs found
The characteristics of free <i>Rg</i> PAL and MCM-41-NH-GA-<i>Rg</i> PAL.
No full text<p>(a) The optimal pH of free <i>Rg</i>PAL and MCM-41-NH-GA-<i>Rg</i>PAL. The effects of pH were determined at 50°C using a series of buffers with various pH values. (b) The pH stability of free and immobilized <i>Rg</i>PAL. The activity was assessed following the enzyme treatment in buffer at different pH values (5.0–12) for 12 h. The relative activity at pH 8.5 was defined as 100%. (c) The optimal temperature of free and MCM-41-NH-GA-<i>Rg</i>PAL. (d) The thermostability of free <i>Rg</i>PAL and MCM-41-NH-GA-<i>Rg</i>PAL. The free <i>Rg</i>PAL and MCM-41-NH-GA-<i>Rg</i>PAL were incubated at 60°C for 10–60 min. The relative activity before incubation was defined as 100%. The values presented correspond to the mean values of at least three replicates.</p
Structural properties of <i>Rx</i>PAL by homology modeling.
No full text<p>Ribbon representation of the homotetrameric model of <i>Rx</i>PAL (Panel A) and backbone line representation model with the MIO (in pink) surrounded by three pairs of cysteines (in yellow) which may form two intra-subunit and one inter-subunit disulfide bridges (Panel B). The electrostatic surface potential representation of <i>Rx</i>PAL (Panel C) compared to that of less alkalophilic <i>Av</i>PAL (Panel D, PDB code 3CZO <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085943#pone.0085943-Wang1" target="_blank">[35]</a>) indicated highly negatively charged <i>Rx</i>PAL surface.</p
Expression and purification of <i>Rx</i>PAL.
No full text<p>Bacterial cells transformed with the pBAD-HisB plasmid containing the gene for <i>Rx</i>PAL were extracted and the cell extract was run on 12% SDS-PAGE gels. Left panel shows expression pattern before induction (Lane A) and 10 hours after induction (Lane B). Right panel shows purified <i>Rx</i>PAL protein (Lane C: 300 µg, Lane D: 200 µg, Lane E: 80 µg, Lane F: 40 µg, Lane G: 20 µg).</p
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
The reusability of MCM-41-NH-GA-<i>Rg</i> PAL.
No full text<p>The initial activity of MCM-NH-GA-<i>Rg</i>PAL was measured and then compared with the activity of the used enzyme obtained after its repeated use per day. After each cycle, MCM-41-NH-GA-<i>Rg</i>PAL was immediately filtered, washed with buffer solution and stored at 4°C.</p
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Ionizable amino acid composition, negative charge excess and salt bridge content of <i>Rx</i>PAL compared to other aromatic amino acid ammonia-lyases.
No full texta<p>3D structures used for salt bridge-content evaluation: <i>Rx</i>PAL (homology model from this work), <i>Pl</i>PAL (homology model from ref. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085943#pone.0085943-Daniel1" target="_blank">[55]</a>), <i>Rt</i>PAL (PDB code: 1Y2M), <i>Pp</i>HAL (PDB code: 1GKM), <i>Pc</i>PAL (PDB code: 1W27), <i>Rs</i>TAL (PDB code: 2O7E), <i>Av</i>PAL (PDB code: 3CZO), <i>Np</i>PAL (PDB code: 2NYF).</p
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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