1,722,145 research outputs found
<i>fan</i> encodes zebrafish Wdr43/Utp5.
No full text<p>(A) Left side: Chromosomal position of the <i>fan</i> locus (left). Sequencing trace data of wild type and <i>fan</i> mutant alleles, with red arrow indicating a premature stop codon in <i>fan</i> mutants (right). Comparison of the conserved nature of the amino acid mutated in <i>fan</i> zebrafish (purple) (middle). Schematic of the Wdr43 protein domains and <i>fan</i> mutant predicted premature stop codon (bottom). (B–E) Live images of zebrafish treated with control MO (CMO) or <i>fan</i> MO at indicated developmental times. (F–I) Alcian blue stained wild type (F, H) and <i>fan</i> mutant (G, I) embryos injected at the single cell stage with GFP control (<i>ctr</i>) or wild type <i>wdr43</i> mRNA. (I) Injected <i>wdr43</i> mRNA rescued <i>fan</i> mutant cartilage formation. (J) Percentage of control and <i>wdr43</i> mRNA injected <i>fan</i> mutant clutches exhibiting wild type, intermediate and <i>fan</i> mutant pharyngeal arch cartilage formation. Numbers of injected embryos scored are indicated at the top of each bar.</p
Phenotype of <i>fan</i> mutants.
No full text<p>(A–C′) Live images of developmentally staged wild type (A–C) and <i>fan</i> mutant (A′–C′) zebrafish. Arrow in A′ indicates necrotic cells in the presumptive eye region. Bracket in B′ shows necrosis in neural and pharyngeal arch tissues. Arrow in C′ points to the distinct hydrocephaly in <i>fan</i> mutant hind brain ventricles, arrowhead indicates incomplete choroid fissure closure and craniofacial defects. (D–D′) Alcian blue stained pharyngeal arch cartilages in 4dpf wild type (D) and <i>fan</i> mutant (D′). (E–K′) Whole mount ISH images of wild type (E–K) and <i>fan</i> mutants (E′–K′) for neural crest markers at indicated developmental stage. Arrows in (E′–G′) and asterisks in (I′–K′) indicate reduced gene expression in <i>fan</i> mutant embryos. Interestingly, <i>fan</i> mutant embryos exhibit similar <i>crestin</i> expression in the trunk NCCs (asterisks in H, H′) but reduced expression in cranial NCCs. (L–L′) <i>Tg(fli1a:EGFP)/fan</i> mutants (L′) exhibit reduced GFP expression in the pharyngeal arch region as compared to wild type embryos (L).</p
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Inhibition of p53 signaling can partially rescue <i>fan</i> mutant craniofacial defects.
No full text<p>(A–B) Whole mount IHC analysis of p53 expression in wild type (A) and <i>fan</i> mutants (B). (C, D) qRT-PCR analysis of <i>d113p53</i> (C) and <i>mdm2</i> (D) gene in wild type and <i>fan</i> mutants at 24, 48 and 72 hpf. (E–G) Bright field images of live 3 dpf wild type (E), <i>fan</i> mutant (F), and <i>fan</i> mutant injected with p53MO (G). (H) Percent zebrafish exhibiting normal eye development in uninjected (Uninj), control MO (CMO), and p53MO injected embryos. (I–K) TUNEL stained and sectioned wild type (WT) (I), <i>fan</i> mutant (J), and <i>fan</i> mutant injected with p53 MO (K). Arrows indicate apoptotic cells. (L) Statistical analyses revealed significantly reduced apoptosis in <i>fan</i> mutants injected with p53MO. (P value<0.01).</p
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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