1,721,080 research outputs found
In vitro antagonistic activity of Argentinean isolates of Purpureocillium lilacinum on Nacobbus aberrans eggs
Get PDFIn vitro interaction of three Argentinean isolates of Purpureocillium lilacinum (one isolated from a public park and two from agricultural soils) towards Nacobbus aberrans eggs was studied. After seven incubation days, the three isolates showed reproductive fungal structures fully developed whereas 80 to 100 % of the eggs were infected. No significant differences among the three isolates were observed in fungal activity with respect to hatching and parasitism under the assayed test conditions (p > 0.05). The hatching percentage in control plates increased during the evaluation period of the cultures, with no signs of fungal infection, showing significant differences as compared to the plates infected (p < 0.05). Although the three isolates studied could be considered as potential agents for biological control of root-knot nematodes, it is necessary to further study some aspects in a wide range of experimental conditions in the laboratory, greenhouse and field so as to determine their real efficiency.Fil: Gortari, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentin
Biotechnological processes for chitin recovery out of crustacean waste: A mini-review
Get PDFAbstract Background: Chitin is an important natural resource. The annual worldwide production is estimated in approximately 1010-1012 ton. It is produced by arthropods (insects and crustaceans), molluscs and fungi. Its main biological function is structural. Crustacean shells are the most important chitin source for commercial use due to its high content and ready availability. Chitin and its derivatives have great economical value because of their numerous applications: food, cosmetics, pharmaceuticals, textile industries, waste water treatment and agriculture. In nature, chitin is closely associated with proteins, minerals, lipid and pigments, which have to be removed. Results: Several techniques to extract chitin from different sources have been reported. The most common method for recovery of chitin from crustacean shells is the chemical procedure. It involves two mayor steps: elimination of inorganic matter (demineralization) and extraction of protein matter (deproteination) using strong acids and bases. However, these processes may cause depolymerization affecting the polymer properties such as molecular weight, viscosity and degree of acetylation. In addition, the chemical purification of chitin is hazardous, energy consuming and threatening to the environment. As an alternative to the chemical process, different biological processes have been investigated: microbiological fermentation and methodologies using enzymatic crude extracts or isolated enzymes. Conclusions: The results reported are extremely variable; however, they offer new perspectives for the production of chitin with the concomitant reduction of the environmental impact.Fil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales (i); ArgentinaFil: Gortari, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales (i); Argentin
Yerba mate as a novel inducer for fungal chlorogenate hydrolase production
Get PDFThe potential of yerba mate (Ilex paraguariensis) to induce chlorogenate hydrolase (EC 3.1.1.42, CHase) activity in different fungal strains has been investigated. CHase was highly induced in mycelia of Aspergillus niger AKU 3302 grown in Czapek medium containing yerba mate extract as inducer. Different materials derived from yerba mate processing were examined as CHase activity inducers. All yerba mate samples produced CHase activity and a concentrated aqueous yerba mate extract was selected as the most convenient inducer. A response surface methodology was used to assess the simultaneous effect of inducer concentration and induction duration on enzyme production. The experimental design revealed that the optimum CHase production was achieved after long induction times (≥25 h) and low inducer concentrations (0.1–0.2% w/v, dry basis).Fil: Butiuk, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; ArgentinaFil: Adachi, Osao. Yamaguchi University; JapónFil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentin
Enzymatic hydrolysis of gelatin layers of X-ray films and release of silver particles using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876
Get PDFEnzymatic decomposition of gelatin layers on used X-ray films and repeated utilization of the enzyme for potential application in silver recovery were investigated using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876. At pH 9.0, the enzymatic reaction was enhanced by the increase of enzyme concentration or by the increase of the temperature up to 60oC. Under the conditions of 6.9 U/ml, 60oC, and pH 9.0, hydrolysis of the gelatin layers and the resulting release of silver particles were achieved within 6 min. The protective effect of polyols against thermal denaturation was investigated. The presence of glycerol and propylene glycol increased enzyme stability. When the reusability of the enzyme for gelatin hydrolysis was tested, it could be seen that it could be effectively reused for more cycles when glycerol was added, compared with the enzyme without protective agents. The results of these repeated treatments suggested that a continuous process of recycling silver from used X-ray is feasible. Keeping in mind that recycling is (at the present time) needed and imperative, it can be remarked that, in this research, three wastes were successfully used: hair waste in order to produce serine proteases; glycerol in order to enhance enzyme thermal stability; and used Xray films in order to recover silver and PET films.Fil: Cavello, Ivana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentin
Bioprocessing of “Hair Waste” by Paecilomyces lilacinus as a Source of a Bleach-Stable, Alkaline, and Thermostable Keratinase with Potential Application as a Laundry Detergent Additive: Characterization and Wash Performance Analysis
Get PDFPaecilomyces lilacinus (Thom) Samson LPS 876, a locally isolated fungal strain, was grown on minimal mineral medium containing “hair waste,” a residue from the hair-saving unhairing process, and produced a protease with keratinolytic activity. This enzyme was biochemically characterized. The optimum reaction conditions, determined with a response surface methodology, were 60°C and pH 6.0. It was remarkably stable in a wide range of pHs and temperatures. Addition of Ca2+, Mg2+, or sorbitol was found to be effective in increasing thermal stability of the protease. PMSF and Hg2+ inhibited the proteolytic activity indicating the presence of a thiol-dependent serine protease. It showed high stability toward surfactants, bleaching agents, and solvents. It was also compatible with commercial detergents (7 mg/mL) such as Ariel, Skip, Drive, and Ace, retaining more than 70% of its proteolytic activity in all detergents after 1 h of incubation at 40°C. Wash performance analysis revealed that this protease could effectively remove blood stains. From these properties, this enzyme may be considered as a potential candidate for future use in biotechnological processes, as well as in the formulation of laundry detergents.Fil: Cavello, Ivana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentin
Informe de personal de apoyo: Gortari, María Cecilia (2012-2013)
No full textProyectos de investigación en los cuales colabora:
a) Hongos autóctonos de posible interés económico: aislamiento, identificación, caracterización molecular y su potencial como productores de enzimas con implicancias biotecnológicas (PIP 112-200801-01422) del CONICET. Director: Dra. Angélica Arambarri. Co-director: Dr. Roque A. Hours.
b) Enzimas, microorganismos y procesos de interés biotecnológico. Acreditado y financiado por la SeCyt de la UNLP, código 11/X522. Años 2009-2012. Director: Dr. Roque A. Hours.
c) Proyecto de Investigación Plurianual (PIP 112-201101-00662) del CONICET: Microorganismos, enzimas y procesos biotecnológicos. Res. 1672/12. Años: 2012-2014
Reduction of l-phenylalanine in protein hydrolysates using l-phenylalanine ammonia-lyase from Rhodosporidium toruloides
Get PDFl-Phenylalanine ammonia-lyase (PAL, EC 4.3.1.25) from Rhodosporidium toruloides was utilized to remove l-phenylalanine (l-Phe) from different commercial protein hydrolysates. A casein acid hydrolysate (CAH, l-Phe ~2.28 %) was employed as a model substrate. t-Cinnamic acid resulting from deamination of l-Phe was extracted, analyzed at λ = 290 nm, and used for PAL activity determination. Optimum reaction conditions, optimized using successive Doehlert design, were 35 mg mL−1 of CAH and 800 mU mL−1 of PAL, while temperature and pH were 42 °C and 8.7, respectively. Reaction kinetics of PAL with CAH was determined under optimized conditions. Then, removal of l-Phe from CAH was tested. Results showed that more than 92 % of initial l-Phe was eliminated. Similar results were obtained with other protein hydrolysates. These findings demonstrate that PAL is a useful biocatalyst for l-Phe removal from protein hydrolysates, which can be evaluated as potential ingredients in foodstuffs for PKU patients.Fil: Castañeda, María Teresita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Adachi, Osao. Yamaguchi University; JapónFil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina. Universidad Tecnológica Nacional; Argentin
Aportes para la degradación biológica de residuos sólidos de curtiembre
No full textEl proceso de depilado conservador del pelo tiene como beneficio hacia el medio ambiente una disminución de la contaminación en el efluente líquido de ribera, pero como contraparte, durante este procedimiento se genera un nuevo residuo sólido llamado "residuo pelo" que debe ser apropiadamente dispuesto.Fil: Cavello, Ivana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Galarza, Betina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo del Cuero; ArgentinaFil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Cantera, Carlos. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo del Cuero; Argentin
Pentose Oxidation by Acetic Acid Bacteria Led to a Finding of Membrane-Bound Purine Nucleosidase
Get PDFD-Ribose and 2-deoxy-D-ribose were oxidized to 4- keto-D-ribonate and 2-deoxy-4-keto-D-ribonate respectively by oxidative fermentation, and the chemical structures of the oxidation products were confirmed to be as expected. Both pentoses are important sugar components of nucleic acids. When examined, purine nucleosidase activity predominated in the membrane fraction of acetic acid bacteria. This is perhaps the first finding of membrane-bound purine nucleosidase.Fil: Adachi, Osao. Yamaguchi University. Faculty of Agriculture. Department of Biological Chemistry; JapónFil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales (i); ArgentinaFil: Akakabe, Yoshihiko. Yamaguchi University. Faculty of Agriculture. Department of Biological Chemistry; JapónFil: Shinagawa, Emiko. Ube National College of Technology. Department of Chemical and Biological Engineering; JapónFil: Ano, Yoshitaka. Ehime University. Faculty of Agriculture. Department of Applied Bioscience; JapónFil: Yakushi, Toshiharu. Yamaguchi University. Faculty of Agriculture. Department of Biological Chemistry; JapónFil: Matsushita, Kazunobu. Yamaguchi University. Faculty of Agriculture. Department of Biological Chemistry; Japó
IN VITRO ACTIVITY OF PURPUREOCILLIUM LILACINUM ISOLATES AGAINST PHYTOPATHOGENIC FUNGI OF SORGHUM
No full textMetabolites produced by antagonist on the growthof phytopathogens fungus. The three P. lilacinumisolates had variable inhibitory activity accordingto the type of assay and phytopathogen evaluated.However, the inhibition was more evident with P.lilacinum Ls isolate and mainly with the effectof the non-volatile compounds. This studysuggests that P. lilacinum could be considered apromising antagonist in the control of Alternaria.These results motivate us to go deeper in theproduction, isolation and identification of thecompounds produced by this specie. The studiedP. lilacinum Ls isolate could be another tool in thedifferent biological control strategies applied tothe conservation of sorghum grains.Fil: Gortari, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Astoreca, Andrea Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentin
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