6,131 research outputs found

    Fu Describing About the Author Himself: A Focus on Ban Gu "You tong fu"

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    From Later Han dynasty onwards, the number of fu describing about the author himself has increased. There are several possible reasons for this, but the most important reason is probably that there was no other genre through which one could express oneself except for fu in this period. For instance, the fiveword poetry was still in the middle way of establishing its own style at that moment. Moreover, in Former Han, there were few opportunities for authors to talk about themselves in detail in fu, because the main readers were emperors and kings of countries. In Later Han, the readers had become more diverse. That is probably the reason why more fu began to mention about the authors themselves in this period. Ban Gu (32-92), who is a representative scholar of Later Han and also a man of letters, expressed his aim by writing "You tong fu". Its main content is that though one's encounter with a disaster or felicity is not always derived from our behavior, we have to have a strong will and take action to overcome disasters. This idea is supported by Ban Gu's experiences of not attaining his aim. Such experiences are common in Chinese authors. One of the earliest examples is Chu Ci. Also, Chu Ci is the one of the origins of fu. It is surprising that authors expressed things about themselves mostly in fu describing journeys. It may be easier to understand this tendency if we think they substituted the heaven wander in Chu Ci with a ground journey. Chu Ci was a model for authors to express their aim which could not come true, even if the journey on the ground and the wander in heaven are different

    Online Iterative Adaptive Dynamic Programming Approach for Solving the Zero-Sum Game for Nonlinear Continuous-Time Systems with Partially Unknown Dynamics

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    The current study presents an online iterative adaptive dynamic programming approach to resolve the zero-sum game (ZSG) for nonlinear continuous-time (CT) systems containing a partially unknown dynamic. The Hamilton-Jacobian-Issacs (HJI) equation is solved along the state trajectory according to the value function approximation and the policy improvement online. Relaxed dynamic programming is utilized to ensure the algorithm’s convergence. Model and costate networks were established to conduct the method. Computational simulations are performed to present the efficiency of the algorithm.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Control & Simulatio

    Note of congratulations from Jack Huang to Dr. Frank Fu, January 1, 1983

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    A note of congratulations to Dr. Frank H. Fu (Fu Haojian) from Springfield College Alum, Jack Huang, Class of 1936, dated January 1, 1983. The card has country lane scene on the front, with a tree with pink leaves, a buttfly, and a tree with its leaves still green. There is the roof of a house just visible over the hill. It also says Congratulations. Inside is short message.Mr. Huang wrote a short personal note congratulating him for his efforts and the recent recognition by then President Ronald Reagan, or the actor as he refers to him, and saying how proud the Chinese are of him. An author of 17 textbooks and more than 100 journal articles, Frank H. Fu, G’73, DPE’75, has worked across the world in China, Canada, and the United States. Throughout his career, he has received many distinguished honors, including the Medal of Honor in 2009 by the SAR Government of Hong Kong. Fu has held positions at institutions such as the Chinese University of Hong Kong and Springfield College, where he served as director of the International Center starting in 1978. Five years later, Fu returned to Hong Kong, but has since remained actively involved with Springfield College, participating in talks on the campus and even hosting a gathering of nearly 60 alumni in Hong Kong. Currently, Fu is the associate vice president of Hong Kong Baptist University, where he also works as the director of the Dr. Stephen Hui Research Centre of Physical Recreation and Wellness. In addition, Fu is president of the Society of Chinese Scholars on Exercise Physiology and Fitness, chairperson of Hong Kong Coach Education Committee, a research fellow of the Research Consortium of SHAPE America, and an international fellow of the National Academy of Kinesiology

    Practical application of on-line partial discharge monitoring technique on 500kV shunt reactor

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    Considering the damage mechanism of oil-impregnated paper insulation in power transformers, shunt reactors and other high voltage electrical apparatus caused by partial discharge, a concept of “destructive partial discharge” is introduced in this paper. The intensity of this discharge is regarded as several thousands pico-coulomb (pC) and may cause the insulation a fatal damage. An oil-paper insulation is usually able to withstand this type of partial discharge for a period of time prior to failure. This provides engineers a time window to detect it. This paper describes an on-line partial discharge monitoring system for 500kV shunt reactors. The commission results from 3 single-phase shunt reactors either connected or disconnected to the grid showed that the on-line partial discharge detecting system has a high noise immunising ability. Two years later after the installation, a pre-warning signal was received from one shunt reactor indicating the existence of an intermittent discharge. The acoustic emission system located its position at the low end of the high voltage bushing in the oil. Dissolved gasses analysis (DGA) in the oil suggested the presence of partial discharge, as acetylene (C2H2) was as high as 20ppm. PD activity was further confirmed by a physical examination on the reactor

    Lu Yu and Tu Fu

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    Lu Yu 陸游 (1125-1209), the famous patriot poet of the Southern Sung, admired Tu Fu above all the poets of the past, and the deep influence which Tu Fu exercised on him may be seen throughout his work. In spite of this fact, however, there are many differences which mark the lives and works of the two men. As a first step towards a comparative study of the two poets, the author has attempted to discover how Lu Yu evaluated his predecessor, using evidence found in Lu Yu's poems themselves. By Lu Yu's time, Tu Fu was already widely recognized as the greatest Chinese poet. It had become a popular pastime to try to identify individual poems of Tu Fu on the basis of isolated phrases or couplets, or to compose poems made up of clever imitations of various couplets of Tu Fu. But Lu Yu, unlike most of his contemporaries, was less attracted by Tu Fu's poetic technique than by his poetic spirit, and regarded the latter as the most valuable lesson to be learned. He insisted that it was Tu Fu the man rather than Tu Fu the poet, Tu Fu the patriot rather than Tu Fu the artist, who deserved to be most highly admired. This was the foundation upon which Lu Yu's evaluation of Tu Fu rested, and the premise upon which he approached his work

    Dr. Frank Fu

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    A portrait photograph of Dr. Frank H. Fu (Fu Haojian). The writing on the photograph says "17 yrs."An author of 17 textbooks and more than 100 journal articles, Frank H. Fu, G’73, DPE’75, has worked across the world in China, Canada, and the United States. Throughout his career, he has received many distinguished honors, including the Medal of Honor in 2009 by the SAR Government of Hong Kong. Fu has held positions at institutions such as the Chinese University of Hong Kong and Springfield College, where he served as director of the International Center starting in 1978. Five years later, Fu returned to Hong Kong, but has since remained actively involved with Springfield College, participating in talks on the campus and even hosting a gathering of nearly 60 alumni in Hong Kong. Currently, Fu is the associate vice president of Hong Kong Baptist University, where he also works as the director of the Dr. Stephen Hui Research Centre of Physical Recreation and Wellness. In addition, Fu is president of the Society of Chinese Scholars on Exercise Physiology and Fitness, chairperson of Hong Kong Coach Education Committee, a research fellow of the Research Consortium of SHAPE America, and an international fellow of the National Academy of Kinesiology.Photograph is stapled with other photographs to folder of original student record kept by the International center

    Dr. Frank Fu thinking

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    A photograph of Dr. Frank H. Fu (Fu Haojian). He is seated with hands held up to his chin and appears to be deep in thought. There are others around him, but they are unable to be seen clearly enough to identify.An author of 17 textbooks and more than 100 journal articles, Frank H. Fu, G’73, DPE’75, has worked across the world in China, Canada, and the United States. Throughout his career, he has received many distinguished honors, including the Medal of Honor in 2009 by the SAR Government of Hong Kong. Fu has held positions at institutions such as the Chinese University of Hong Kong and Springfield College, where he served as director of the International Center starting in 1978. Five years later, Fu returned to Hong Kong, but has since remained actively involved with Springfield College, participating in talks on the campus and even hosting a gathering of nearly 60 alumni in Hong Kong. Currently, Fu is the associate vice president of Hong Kong Baptist University, where he also works as the director of the Dr. Stephen Hui Research Centre of Physical Recreation and Wellness. In addition, Fu is president of the Society of Chinese Scholars on Exercise Physiology and Fitness, chairperson of Hong Kong Coach Education Committee, a research fellow of the Research Consortium of SHAPE America, and an international fellow of the National Academy of Kinesiology

    Dr. Frank Fu, c. 1979

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    A portrait photograph of Dr. Frank H. Fu (Fu Haojian). The photograph was used in news stories on Dr. Fu for his work and his appointment as Director of Springfield College's International Center.An author of 17 textbooks and more than 100 journal articles, Frank H. Fu, G’73, DPE’75, has worked across the world in China, Canada, and the United States. Throughout his career, he has received many distinguished honors, including the Medal of Honor in 2009 by the SAR Government of Hong Kong. Fu has held positions at institutions such as the Chinese University of Hong Kong and Springfield College, where he served as director of the International Center starting in 1978. Five years later, Fu returned to Hong Kong, but has since remained actively involved with Springfield College, participating in talks on the campus and even hosting a gathering of nearly 60 alumni in Hong Kong. Currently, Fu is the associate vice president of Hong Kong Baptist University, where he also works as the director of the Dr. Stephen Hui Research Centre of Physical Recreation and Wellness. In addition, Fu is president of the Society of Chinese Scholars on Exercise Physiology and Fitness, chairperson of Hong Kong Coach Education Committee, a research fellow of the Research Consortium of SHAPE America, and an international fellow of the National Academy of Kinesiology

    The study of Lu Fu in the early Song Dynasty

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    碩士一、研究目的: 本文擬從歷代賦學審美意識中,觀看宋初律賦的內容。包括破題、音律、對偶,以及影響宋初律賦主題的因素。 二、研究範圍: 時間上,由宋太祖趙匡胤陳橋兵變,建年號建隆元年(960)開始,歷太宗、真宗,至宋仁宗親政的明道元年(1032)止,作為本文時間的依據。 文本的選擇,此時期文士所撰寫的律賦,無論是應制之作、科舉考試之律賦,或是習作等,都是本文討論的範圍。然本文以單篇律賦為主,吳淑著《事類賦注》一百篇雖皆屬律賦,然因其為類書,且意義獨具,當另以專書論述。 依作者生年及登進士第的順序,參閱《現存宋人著述總錄》、《全宋文》、《歷代賦彙》,共錄律賦一百一十六篇。 三、研究方法: 律賦常為後人所詬病,筆者由傳統賦學的審美觀,自漢賦、曹丕《典論‧論文》、陸機《文賦》、鍾嶸《詩品》,賦的審美意識皆與各時代的文風及流行文體或政治風氣有所相涉,至劉勰《文心雕龍‧詮賦》篇,將賦體的特色獨立,也確立了名為賦體所該擁有的價值。 將傳統的審美意識帶入律賦中,探討宋初律賦是否合乎賦的標準,便是本文所著重的。其次則是在宋代初年的政治風氣及文學風氣中,律賦何以成為科舉考試之要項。本文將由演繹方式,例舉代表宋初律賦之作家,羅列以證宋初律賦之特色。 四、研究結論: 筆者認為律賦之美,不僅是因其題下限韻,因難而巧。更難能可貴的是律賦的破題、對偶及音律之美,讀之錯落有致,更添美感。希望本文可作為拋磚引玉之效,使律賦復其文學之地位,也可由本文得知宋初律賦與唐代及後世律賦的獨特之處。Abstract: The purpose of the study: This study is trying to analyze the content of Lu Fu in the early Song dynasty, including its introductory, rhythm, symmetry and the theme of the verse. The scope of the study: The research materials are selected within four kingdoms period, Emperor Taizu, Emperor Taizong, Emperor Zhenzong and Emperor Renzong, from 960 B.C. to 1032 B.C.; and the contents are chosen from imperial examinations in former times and personal practice writing. Although this study is focusing on independent Fu, Shi Lei Fu Ju, a very important work, written by Shu Wu is a tool book consisting a hundred correlated Fu which can not be left out when discussing Fu in this study. Total 116 Fu are picked in this study and according to Comprehensive Index of Extant Works by Song Persons, Chuan Song Wen and Li Dai Fu Lu, those pomes are listing by year of author’s birth or year that a author success in the highest imperial examinations. Methodology: This study makes generality comments on Lu Fu in Early Song dynasty based on traditional aesthetic and literary criticism book, for example Han Fu, A Discourse on Literature by Pi Cao, Wen Fu by Ji Lu and Shi Ping by Ron Chung. The aesthetic on Fu was changing by the literature trend and the political situation in different dynasty. Until the first work of aesthetics and also the first systematic work of literary criticism, The Literary Mind and the Carving of Dragon by Xie Liu, Fu formed a unique characteristic and style of its own and became a major poetic genre. Comparing to the former aesthetic views to look for if Lu Fu match to the traditional criterion is the key emphasis in this study. The second, this study is trying to find out why Lu fu became a main stream in the imperial examination in the Song dynasty. This research using the approach of natural deduction, brining up several famous writers to proof the magnificent features of Lu Fu. Conclusion: The limitation of rhyme scheme makes Lu fu very difficult to compose, but the perplexity of Lu Fu brings out the true beauty within the verse. Furthermore, when reading a Lu Fu, readers can appreciate the beautiful introductory, symmetry and rhyming pattern of it. Hoping through study, Lu Fu can be rehabilitated its reputation and status in Chinese literature history.目錄 第一章 緒論 1 第一節 研究動機 2 第二節 研究範圍 4 一、宋初律賦文本 7 二、宋初律賦作者 13 (一)、田錫 14 (二)、王禹偁 14 (三)、王曾 14 (四)、夏竦 15 (五)、范仲淹 15 (六)、宋庠 15 (七)、宋祁 15 (八)、文彥博 16 三、宋初考試制度相關資料 16 第三節 研究方法 21 第二章 賦的功能與審美要求 23 第一節 賦的功能 23 第二節 賦的審美要求 29 一、鋪采摛文 30 二、體物寫志 33 三、音律流麗 39 第三節 結語 42 第三章 律賦的形成及發展 44 第一節 律賦「新體」的特徵 44 第二節 律賦的句式 47 第三節 律賦之用韻 52 第四節 律賦之使事 56 第五節 律賦遭詬病的原因 60 第六節 結語 63 第四章 宋初律賦的特色 65 第一節 宋人賦論 66 第二節 律賦章法 72 第三節 律賦押韻 77 第四節 律賦的對偶與使事 80 第五節 結語 82 第五章 宋初律賦的內容 84 第一節 宋初律賦的內涵 84 一、田錫 84 二、王禹偁 86 三、夏竦 87 四、范仲淹 89 五、宋庠、宋祁 92 六、文彥博 93 第二節 宋初律賦的題材 94 第三節 影響律賦主題的因素 99 一、矛盾與沉潛 100 二、儒學的復興 104 三、釋道的交融 112 第四節 結語 116 第六章 結論 118 參考文獻 121學號: 695010305, 學年度: 9

    Subunit Interaction of the 26S Proteasome

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    26S蛋白解體(proteasome)於真核細胞中之ubiquitin/proteasome pathway中扮演著調控蛋白質分解的重要角色。26S蛋白解體是由20S主體(core particle; CP)及19S調控體(regulatory particle; RP)兩個亞體(subcomplex)所組成。20SCP為主要分解去折疊之蛋白質的部分,而19S RP一般認為是扮演辨識宇元化之蛋白受質、蛋白質去折疊(unfolding)及轉送(translocation)去折疊之蛋白質進入20S CP的角色。為了進一步瞭解19S RP的功能與機制,本實驗以酵母菌雙混雜法(two-hybrid)來分析26S蛋白解體次單元之交互作用的關係,初步推測其三級結構之分佈情形,希望對日後了解26S蛋白解體分解蛋白質的機制有所幫助。 本實驗以酵母菌26S蛋白解體為模式,分離其18個RP次單元基因及7個α次單元基因,分別構築於酵母菌雙混雜載體,於酵母菌系YRG2內分析18個RP次單元之間的交互作用(共有18 ×18個測試組合)及分析7個α次單元與18個RP次單元之間的交互作用(共測試了3 ×7 ×18個可能組合)。RPN10/MCB1(Multiubiquitin Chain Binding Protein)為19S RP中一個次單元,是唯一可在生體外(in vitro)與宇元鏈(ubiquitin chain)結合的次單元。但RPN10減基因突變對酵母菌及Physcomitrella patens(moss)並不會造成致死現象,所以RPN10可能參與其它生理功能。為瞭解RPN10於生體內真正的生理功能及其分子作用機制,同時也利用酵母菌雙混雜法,以P. paterns之RPN10(PpRPN10)篩選由P. patens所製備的雙混雜cDNA基因庫,以篩選及分離與RPN10具有交互作用之基因。 本實驗所測試的19S RP次單元間之測試組合中,成功獲得5個組合具交互作用,其中3組已有文獻記載;另2組則目前並無文獻記載,為新發現之組合。另外本實驗20S CP 的7個α次單元與19S RP 18個次單元的測試組合,在HIS3報導基因的表現上並無正反應。當以P. paterns之RPN10來篩選P. paterns之cDNA基因庫可成功地獲得一NTI1(RPN Ten Interaction Factor)因子基因,此基因蛋白產物經過序列比對,與阿拉伯芥ribosomal protein s29-like的相似度(similarity)為80%、相同(identity)為74%;與人類之ribosomal protein s29的相似度為74%、相同為64%;與酵母菌之ribosomal protein s29的相似度為68%、相同為62%。 19S RP次單元間交互作用之組合是在in vivo下成功獲得的,可以進一步確立其19S RP次單元間在三度空間上的關係;而20S CP 7個α次單元與19S RP 18個次單元的測試組合中並無結果。此結果似乎可以說明當7個α次單元組合成複合體(complex)時,20S CPα次單元與19S RP次單元才有交互作用產生,單獨存在的α次單元無法與19S RP次單元進行交互作用。The 26S proteasome plays a central role in degrading cellular proteins conjugated by ubiquitins. The 26S proteasome complex is composed of sub-complexes: the 20S core particle (CP), contains proteolytic active sites in its lumen and the 19S regulatory particle (RP). The exact functions and mechanisms of the 19S RP are unknown. As a first step towards discovering the precise functions of the 19S RP, we have proposed here to investigate its three dimensional structure, i.e. how the individual subunits are organized, by molecular genetic approaches. To serve this purpose, the two-hybrid system was employed here using the yeast 26S proteasome as a model. We isolated genes encoding all 18 RP and 7 CPα—subunits from yeast and constructed separately the isolated RP and CP subunit genes into the yeast two-hybrid vectors. We examined interaction (18 x 18 combinations) among RP subunits using the established chimeric two-hybrid vectors in the yeast YRG2 strain. Among the 18 x 18 combinations of 19S RP subunits interaction tested, eight of them were found to be positive. Four out of these combinations have been reported elsewhere and other four are novel combinations. We also examined the interactions (3 x 7 x 18 combinations) of the seven CP α—subunits and the eighteen RP subunits. However, no interaction was observed. The lack of one-by-one interaction between CP and RP suggests that more than one subunit of CP and RP is involved in the assembly of the complex 26S proteasome. The RPN10/MCB1 (Multiubiquitin Chain Binding Protein) is an important subunit of 19S RP and is also the only subunit that has affinity for multiubiquitin chains in vitro. The fact that P. patens and yeast Δrpn10 strains were viable indicates that in addition to ubiquitin recognition, RPN10 might have other functions besides ubiquitin/proteasome pathway. The RPN10 of P. patens was used to screen the P. patens cDNA library by employing the two-hybrid system. A factor, NTI1 (RPN Ten Interaction), was obtained. The deduced amino sequence of NTI1 showed 80% homology to the arabidopsis s29 ribosomal protein, 74% homology to the human s29 ribosomal protein, and 68% homology to the yeast s29 ribosomal protein.目 錄 中文摘要…………………………………………………………………….1 英文摘要…………………………………………………………………….2 前言………………………………………………………………………….3 一、ubiquitin/proteasome pathway……………………………………..3 二、ubiquitin/proteasome pathway參與植物代謝生理功能………….6 (一)、光敏素的分解………………………………………………….6 (二)、逆境下蛋白質的turnover……………………………………...8 三、20S core particle (CP)結構與功能………………………………..10 四、19S regulatory particle (RP)結構與功能……………………………12 五、RPN10次單元功能………………………………………………….16 六、實驗主題……………………………………………………………..19 (一)、19S RP次單元之交互作用……………………………………..19 (二)、RPN10的結構與功能分析………………………………………21 1.與19S次單元於間之交互作用…………………………………21 2.篩選其他與RPN10交互作用之因子…………………………...22 材料與方法…………………………………………………………………….23 一、實驗材料……………………………………………………………….23 1.材料………………………………………………………………23 2.酵母菌培養基……………………………………………………23 二、實驗方法……………………………………………………………….24 1.酵母菌染色體組DNA抽取方法……………………………….24 2.分離酵母菌19S RP及20SCP次單元基因…………………….25 3.選殖於中間載體…………………………………………………26 4.大腸桿菌之轉化…………………………………………………27 5.Digestion…………………………………………………………27 6.DNA序列分析…………………………………………………..28 7.酵母菌雙混雜載體……………………………………………..28 8.選殖於雙混雜載體………………………………………………28 9.酵母菌之轉化……………………………………………………29 10. 報導基因的活性分析…………………………………………..30 11. 分離PpRPN10CΔ1…………………………………………….31 12.以pBD::PpRPN10CΔ1篩選由P. patens所建立的雙混雜cDNA基因庫……………………………………………………………32 13.由酵母菌分離雙混雜質體………………………………………32 14.序列分析…………………………………………………………33 結果…………………………………………………………………………….34 一、26S proteasome(蛋白解體)次單元之交互作用分析………….….34 1.整體策略………………………………………………………...34 2.19S RP之18個次單元間的交互作用關係…………………….36 3.20S CP α次單元與19S RP次單元之交互作用……………...37 二、PpRPN10與ribosomal protein s29具交互作用……………………..39 1.以PpRPN10CΔ1 clone為篩選由P. patens所建立的雙混雜cDNA基因庫的材料,並成功獲得13個clone……………40 2.NTI1為ribosomal protein s29………………………………41 討論…………………………………………………………………………….43 一、26S proteasome(蛋白解體)次單元之交互作用…………………...43 二、與RPN10次單元交互作用之因子…………………………………..46 三、辨識交互作用方法…………………………………………………...49 四、酵母菌雙混雜載體…………………………………………………...52 參考文獻……………………………………………………………………….78 圖表及附錄 表一、用來分離酵母菌RP次單元基因之DNA 引子序列………………….54 表二、用來分離酵母菌20S主體α次單元基因之DNA 引子序列……………55 表三、不同之目標基因與其接合構築之載體種類………..…….……………56 表四、利用酵母菌雙混雜法測試19S RP次單元交互作用之Lac Z報導基因活性表現。………………………………………………………….57 表五、利用酵母菌雙混雜法測試20S CPα次單元與19S RP次單元交互作用之Lac Z報導基因活性表現…………………………………….58 圖一、酵母菌雙混雜系統使用之酵母菌雙混雜載體………………………...59 圖二、18個RP次單元基因與Gal4 AD或Gal4 BD序列之融合建構…….60 圖三、7個主體(CP)a次單元基因與Gal4 AD或Gal4 BD序列之融合建構…61 圖四、RP次單元基因之交互作用的測試組合……………………………….62 圖五之一、CP a次單元與RP次單元基因之交互作用的所有可能組合……63 圖五之二、CP a次單元與RP次單元基因之交互作用的所有可能組合……64 圖六、藉由HIS3及Lac Z報導基因的表現分析蛋白質之交互作用………65 圖七、19S次單元與pBD-Gal4 Cam及pAD-Gal4-2.1酵母菌雙混雜載體之羧機端構築對照試驗…………………………………………………66 圖八、7個α次單元分別與pBD-Gal4 Cam及pADCT酵母菌雙混雜載體構築之對照試驗……………………………………………………………67 圖九、RP次單元融合於AD之羧基端與RP次單元融合於BD之羧基端的測試組合………………………………………………………………68 圖十、19S RP次單元交互作用之Lac Z報導基因活性表現……………….69 圖十一、α次單元融合於BD之羧基端與RP次單元融合於AD之羧基端的測試組合………………………………………………………………70 圖十二、α次單元融合於BD之羧基端與RP次單元融合於AD之氨基端的測試組合………………………………………………………………71 圖十三、α次單元融合於AD之氨基端與RP次單元融合於BD之羧基端的測試組合………………………………………………………………72 圖十四、pBD::PpRPN10cΔ1(pBD::Ppmcb1cΔ1)篩選由P. patens所建立的雙混雜cDNA基因庫結果………………………………………….73 圖十五、PpRPN10CΔ1(Ppmcb1CΔ1)分別與NTI1及NTI9交互作用的β-gal比活性表現……………………………………………………..74 圖十六、NTI1之核酸序列及轉譯之氨基酸序列……………………………..75 圖十七、NTI1轉譯氨基酸序列與已知ribosomal protein s29轉譯氨基酸序列比對……………………………………………………………………76 圖十八、19S RP次單元間之交互作用模式圖……………………………….77 附錄一、建構允許目標基因與AD或BD序列形成氨基端融合(N-terminal fusion)之改良式酵母菌雙混雜載體………………………………….9
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