1,720,966 research outputs found
Direct recovery of intracellular proteins from Candida utilis using reverse micelles in combination with reducing agent
No full textIntracellular proteins were directly extracted from Candida utilis using reverse micelles in combination with a reducing agent. Extraction at pH 10 using 80 mM cetyl trimethyl ammonium bromide (CTAB) and 0.2 % 2-mercaptoethanol was the most favorable condition for cell permeabilization and protein solubilization. Stripping at pH 7 in the presence of 0.5 M KCl resulted in the efficient recovery of solubilized proteins
Searching electrocatalysts for methanol oxidation and oxygen reduction in DMFC by combinatorial chemistry
No full text
Development of a fully integrated microfluidic system for sensing infectious viral disease
No full textAn active micromixer system utilizing the magnetic force was developed and examined for its ability to facilitate the mixing of more than two fluid flows. The mixing performance of the active micromixer was evaluated in aqueous-aqueous systems including dyes for visual observation. A complete analytical microfluidic system was developed by integrating various functional modules into a single chip, thus allowing cell lysis, sample preparation, purification of intracellular molecules, and subsequent analysis. Upon loading the cell samples and lysis solution into the mixing chamber, the integrated microfluidic device allows efficient cell disruption by rotation of a micromagnetic disk and control of mixing time using the Teflon-coated hydrophobic film as a microvalve. This inflow is followed by separating the cell debris and contaminated proteins from the cell lysate sample using the acrylamide (AAm)-functionalized SPE. The inflow of partially purified cell lysate sample containing the gold binding polypeptide (GBP)-fusion protein was bound onto the gold micropatterns by means of its metal binding affinity. The GBP-fusion method allows immobilization of proteins in bioactive forms onto the gold surface without surface modification suitable for studying antigen-antibody interaction. It was used for the detection of severe acute respiratory syndrome (SARS), an infectious viral disease, as an example case.This work was supported by the Basic Research Program of
the Korea Science & Engineering Foundation (R01-2006-000-
11175-0 for WHH and TJP) and by the IT Leading R&D Support
Project from the Ministry of Knowledge Economy through
IITA (SYL and TJP). Further supports by the LG Chem Chair Professorship and the KOSEF through the Center for Ultramicrochemical
Process Sytems (SYL) are appreciated. We also
thank K.-B. Lee (Korca Basic Science Institute, Korea) for his
kind advice for electrochemical analysis
Improving Direct Methanol Fuel Cell Performance by Using Combinatorial Electrochemistry and Highly Porous Carbon Support
No full textIn order to improve the performance of direct-methanolfuelcell (DMFC) performance, the combinatorial method using a repeated cyclic voltammetry was used to optimize the metal composition for the anodic material and the highlyporouscarbon. which showed the higher Pt dispersion, was investigated using various characterization tools. The activity of newly-developed electrocatalyst, Pt(77)Ru(17)Mo(4)W(2), whose composition was determined through high-throughput screening was more active and stable than Pt(50)Ru(50) in methanol electrooxidation. The Pt particles supported on highlyporouscarbons (Cgl/USY-1 and Csu/MCM-48-2) synthesized with USY zeolite and MCM-48 silica as a template showed the larger electrochemical active surface areas and the higher Pt dispersions, compared to Pt particles supported on Vulcan XC-72R carbon
LIQUID LIQUID EQUILIBRIA FOR THE SYSTEM WATER TERT-BUTYL ALCOHOL DIISOBUTYLENE AT 25, 45, AND 75-DEGREES-C
No full text
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
- …
