180,020 research outputs found
Effects of HO-1 overexpression on mitochondrial dysfunction and autophagy level in Lv-HO-1-H9c2 cells with H/R model.
No full text(A) Cell viability was measured using the CCK-8 assay. The data are presented as means ± SE (N = 5). **p<0.01 vs corresponding Normoxia group; #p<0.05, vs corresponding Lv-scramble group. (B) Real-time quantitative PCR (RT q-PCR) analyses of HO-1 mRNA expression in lv-HO-1 H9c2 cells subjected to H/R relative to GAPDH expression (n = 3 wells per group). **p<0.01 vs. the normoxia group. (C) H/R-induced HO-1, p62, and LC-3 protein expression analyzed using western blots of lv-HO-1 H9c2 cells. GAPDH was used as a loading control (n = 3 wells per group). ##p<0.01 vs corresponding Lv-scramble group; ***p<0.001 vs. the corresponding normoxia group. (D) Representative confocal microscopy images and quantitative analysis of autophagosomes from 15 fields (n = 3 hearts per group). Scale bar = 500 nm. *p<0.05 vs. the corresponding normoxia group; ##p<0.01, vs corresponding Lv-scramble group. H/R, Hypoxia/reoxygenation; HO-1, heme oxygenase-1. (E,F) Flow cytometry detection of changes in JC-1 fluorescence color reflects changes in the mitochondrial membrane potential and mitochondrial ROS levels. #p<0.05, vs corresponding Lv-scramble group; **p<0.01 vs corresponding Normoxia group, #p<0.05, vs corresponding Lv-scramble group. (G) The apoptosis rate of the four groups. Cell identification and detection of apoptosis. **p<0.01 vs corresponding Normoxia group; ##p<0.01, vs corresponding Lv-scramble group.</p
The 1961 Kampong Bukit Ho Swee fire and the making of modern Singapore
Full text linkBy 1970, Singapore’s urban landscape was dominated by high-rise blocks of planned public housing built by the People’s Action Party government, signifying the establishment of a high modernist nation-state. A decade earlier, the margins of the City had been dominated by kampongs, home to semi-autonomous communities of low-income Chinese families which freely built, and rebuilt, unauthorised wooden houses. This change was not merely one of housing but belied a more fundamental realignment of state-society relations in the 1960s. Relocated in Housing and Development Board flats, urban kampong families were progressively integrated into the social fabric of the emergent nation-state. This study examines the pivotal role of an event, the great Kampong Bukit Ho Swee fire of 1961, in bringing about this transformation. The redevelopment of the fire site in the aftermath of the calamity brought to completion the British colonial regime’s ‘emergency’ programmes of resettling urban kampong dwellers in planned accommodation, in particular, of building emergency public housing on the sites of major fires in the 1950s. The PAP’s far greater political resolve, and the timing of and state of emergency occasioned by the scale of the 1961 disaster, enabled the government to rehouse the Bukit Ho Swee fire victims in emergency housing in record time. This in turn provided the HDB with a strategic platform for clearing other kampongs and for transforming their residents into model citizens of the nation-state. The 1961 fire’s symbolic usefulness extended into the 1980s and beyond, in sanctioning the PAP’s new housing redevelopment schemes. The official account of the inferno has also become politically useful for the government of today for disciplining a new generation of Singaporeans against taking the nation’s progress for granted. Against these exalted claims of the fire’s role in the Singapore Story, this study also examines the degree of actual change and continuity in the social and economic lives of the people of Bukit Ho Swee after the inferno. In some crucial ways, the residents continued to occupy a marginal place in society while pondering, too, over the unresolved question of the cause of the fire. These continuities of everyday life reflect the ambivalence with which the citizenry regarded the high modernist state in contemporary Singapore
Ha Ho, H. Hommel G.m.b.H., Mainz, Werkzeuge und Maschinen
No full textHA HO, H. HOMMEL G.M.B.H., MAINZ, WERKZEUGE UND MASCHINEN
Ha Ho, H. Hommel G.m.b.H., Mainz, Werkzeuge und Maschinen ( -
HO-1 and HIF-1α are expressed in the peri-infarct region of the ischemic mouse brain.
No full text(A) Representative image of the TTC stained regions (a, contralateral region; b, peri-infarct region; c, infarct region) in a mouse subjected to 2 h ischemia and 24 h reperfusion (I/R) (n = 3 per group). (B) DAB staining observed as brown color in the peri-infarct region (b) in wild-type (WT) and HO-1+/- mice. Scale bars = 20 μm. (C) Expression of target proteins was determined in brain tissues using western blot analysis, and their levels were quantified (n = 5 per group). **P D) WT and HO-1+/- mice were subjected to I/R, and the brain sections (a, contralateral region; b, peri-infarct region; c, infarct region) were stained with the indicated antibodies (n = 4 per group). Images are representative from three individual tissues.</p
Study of Ho-doped Bi2Te3 topological insulator thin films
Full text linkThis publication arises from research funded by the John Fell Oxford University Press (OUP) Research Fund and the Research Complex at Harwell is acknowledged for their hospitality. This work was supported by a DARPA MESO project (No. N66001-11-1-4105). S.E.H. was supported by the VPGE (Stanford University). L.C.M. and A.A.B. acknowledge partial financial support from EPSRC (UK) through a Doctoral Training Award. Diamond Light Source is acknowledged for beamtime on I10 (proposal SI10207).Breaking time-reversal symmetry through magnetic doping of topological insulators has been identified as a key strategy for unlocking exotic physical states. Here, we report the growth of Bi2Te3 thin films doped with the highest magnetic moment element Ho. Diffraction studies demonstrate high quality films for up to 21% Ho incorporation. Superconducting quantum interference device magnetometry reveals paramagnetism down to 2 K with an effective magnetic moment of ∼5 μB/Ho. Angle-resolved photoemission spectroscopy shows that the topological surface state remains intact with Ho doping, consistent with the material's paramagnetic state. The large saturation moment achieved makes these films useful for incorporation into heterostructures, whereby magnetic order can be introduced via interfacial coupling.Peer reviewe
Coelorinchus fuscigulus Iwamoto, Ho & Shao 2009
No full text137. Coelorinchus fuscigulus Iwamoto, Ho & Shao, 2009:40, Figs. 1A–E, 2A–B Holotype: ASIZP 70169 (322 TL), 24.94°N, 121.9°E, Tashi, Yilan, northeastern Taiwan, coll. H.–C. Ho, 23 May 2007. Paratypes: ASIZP 63193 (1, 228 TL) and CAS 224492 [ex. ASIZP 63193] (1, 190 TL), 25.75°N, 123.48°E, Diaoyutai Archipelago, Yilan, Taiwan, coll. H.–C. Ho, 24 Apr. 2004. ASIZP 63249 (1, 233 TL), near holotype locality, 21 Mar. 2004. ASIZP 66922 (1, 286 TL), R / V Ocean Researcher I, st. CP248, 24.8656°N, 122.0411°E, 536 m, 28 Aug. 2004. ASIZP 66973 (1, 293 TL), Nanfangao fish market, coll. H.–C. Ho, 26 Jan. 2007. ASIZP 70168 (1, 301+ TL) and CAS 228337 [ex. ASIZP 70168] (2, 285+–302+ TL), near holotype locality, 29 Jun. 2007. CAS 228338 [ex. CAS 224583, in part] (1, 266 TL), Nanfangao fish market, coll. H.– C. Ho.Published as part of Ho, Hsuan-Ching & Shao, Kwang-Tsao, 2011, 2957, pp. 1-74 in Zootaxa 2957 on page 3
HMOX1 gene promoter alleles and high HO-1 levels are associated with severe malaria in Gambian children.
Full text linkHeme oxygenase 1 (HO-1) is an essential enzyme induced by heme and multiple stimuli associated with critical illness. In humans, polymorphisms in the HMOX1 gene promoter may influence the magnitude of HO-1 expression. In many diseases including murine malaria, HO-1 induction produces protective anti-inflammatory effects, but observations from patients suggest these may be limited to a narrow range of HO-1 induction, prompting us to investigate the role of HO-1 in malaria infection. In 307 Gambian children with either severe or uncomplicated P. falciparum malaria, we characterized the associations of HMOX1 promoter polymorphisms, HMOX1 mRNA inducibility, HO-1 protein levels in leucocytes (flow cytometry), and plasma (ELISA) with disease severity. The (GT)(n) repeat polymorphism in the HMOX1 promoter was associated with HMOX1 mRNA expression in white blood cells in vitro, and with severe disease and death, while high HO-1 levels were associated with severe disease. Neutrophils were the main HO-1-expressing cells in peripheral blood, and HMOX1 mRNA expression was upregulated by heme-moieties of lysed erythrocytes. We provide mechanistic evidence that induction of HMOX1 expression in neutrophils potentiates the respiratory burst, and propose this may be part of the causal pathway explaining the association between short (GT)(n) repeats and increased disease severity in malaria and other critical illnesses. Our findings suggest a genetic predisposition to higher levels of HO-1 is associated with severe illness, and enhances the neutrophil burst leading to oxidative damage of endothelial cells. These add important information to the discussion about possible therapeutic manipulation of HO-1 in critically ill patients
Sung-Ho Jin
No full text학위논문(박사)--아주대학교 일반대학원 :의학과,2014. 2ABSTRACT ⅰ
TABLE OF CONTENTS ⅲ
LIST OF FIGURES ⅴ
LIST OF TABLES ⅶ
Ⅰ. INTRODUCTION 1
A. Gastric Cancer and Malignant Ascites 1
B. Intraperitoneal Radioimmunotherapy 3
C. Purpose of the Study 6
Ⅱ. MATERIALS AND METHODS 7
A. Materials 7
1. Cell Lines 7
2. Subcutaneous Tumor and Malignant Ascites Mouse Models 7
3. Preparation of Radioimmunoconjugates 8
B. Methods 10
1. Western Immunoblot Analysis 10
2. Flow Cytometry Analysis 11
3. In vitro Cytotoxicity of Cetuximab 12
4. Immunofluorescence Microscopy 12
5. In vitro Stability Study of 64Cu-DOTA-Bn-NCS-Cetuximab 13
6. Immunoreactivity Assay 13
7. MicroPET Imaging 14
8. Biodistribution Studies 15
9. Peritoneal Membrane Autoradiography 15
10. Statistical Analysis 16
Ⅲ. RESULTS 17
A. EGFR Expression Levels in Gastric Carcinoma Cells 17
B. Subcellular Localization of Cetuximab-bound EGFR 19
C. Cetuximab Cytotoxicity in Gastric Cancer Cells 20
D. Conjugation and 64Cu Labeling 21
E. In Vitro Properties of 64Cu-DOTA-Cetuximab 23
F. Bilateral Subcutaneous Tumor and Malignant Ascites Mouse Models 26
G. PET Imaging of Bilateral Subcutaneous Tumor Model Mice 28
H. Biodistribution of 64Cu-DOTA-Cetuximab in Bilateral Subcutaneous Tumor Model Mice 29
I. EGFR expression level affected by incubation periods 33
J. PET Imaging of Malignant Ascites Model Mice after I.V. or I.P. Administration of 64Cu-DOTA-Cetuximab 35
K. Biodistribution of 64Cu-DOTA-Cetuximab in Malignant Ascites Model after I.V. or I.P. Administration 37
L. Localization of 64Cu-DOTA-Cetuximab in Peritoneal Seeding 43
Ⅳ. DISCUSSION 44
Ⅴ. CONCLUSION 49
REFERENCES 50
국문요약 61MasterIntraperitoneal (i.p.) radioimmunotherapy (RIT) may be a promising treatment strategy for intraperitoneally-confined malignant diseases including malignant ascites (MA) in gastric cancer. We conducted a comparative biodistribution study of 64Cu-DOTA-cetuximab in subcutaneous (SQ) tumor and MA mouse models.
A mouse xenograft model of bilateral SQ tumors that express epidermal growth factor receptor (EGFR) was established by SQ injections of NUGC-4 and MKN-45 gastric cancer cells into either shoulder of female BALB/c nude mice. The MA model was produced by i.p. injection of NUGC-4 cells. Cetuximab was conjugated to a bifunctional chelator (p-SCN-Bn-DOTA) and labeled with Copper-64 (T1/2 = 12.7 hours). Radiolabeled cetuximab was then administered intravenous (i.v.) or i.p. injection. Positron emission tomography (PET) imaging and biodistribution studies were conducted at the indicated time points.
In the bilateral SQ tumor model, the peak uptake of radiolabeled cetuximab in the NUGC-4 and MKN-45 tumors was detected at 24 h post-injection of 64Cu-DOTA-cetuximab by PET imaging and biodistribution analysis. NUGC-4 tumor uptake of radiolabeled cetuximab was significantly higher than MKN-45 tumor uptake at all time points. The EGFR expression levels of the two cell lines were similar according to in vitro experiments. However, based on flow cytometry analyses at different incubation times with primary antibody (cetuximab) ranging from 3 min to 3 h, the EGFR expression levels were increased over time, and the increasing slope was higher in NUGC-4 cells than in MKN-45 cells.
In the i.v.-induced MA model mice, the biodistribution of radiolabeled cetuximab in the blood pool and liver was high at 1 h and 3 h after i.v. injection and decreased over time. In the i.p.-induced MA model mice, the percent injected dose per gram (% ID/g) of ascites was very high and more than 8% at 1, 3, 24, and 48 h post injection; however, the % ID/g in all normal organs was equal to or less than 6% at all time points. In addition, all peak uptakes of normal organs and i.p.-induced NUGC-4 tumors appeared at 24 h after injection. In addition, the uptake ratios of tumor, peritoneum, and ascites to normal organs after i.p. injection were greater than after i.v. injection, particularly at the early time points. Finally, autoradiography studies using excised peritoneum and mesentery indicated several hot spots corresponding to peritoneal seeding.
The results suggest that using the i.p. route for RIT in malignant ascites models shows advantages over the i.v. route with regard to tumor targeting and half-life prolongation of radioimmunoconjugates in the peritoneal cavity
Additions to the moss flora of Endau Rompin National Park, Johore State, peninsular Malaysia
No full textIn a recent survey of the Endau Rompin National Park (ERNP) in Johore State, 81 species and 4 varieties of mosses were documented. This increases the previous count from 62 species and 3 varieties of mosses in ERNP to 111 species and 5 varieties in total. Of these, 30 species are new records for Johore State. Rhaphidostichum bunodicarpum and Trichosteleum stigmosum are two species new to Peninsular Malaysia. Thuidium assimile is a new record for West Malesia. A new combination, Papillidiopsis aquaticum (Dix.) Boon-Chuan Ho & B.C. Tan is proposed. In terms of species composition, the pan-tropical families of Calymperaceae, Fissidentaceae, Leucobryaceae and Sematophyllaceae predominate the moss flora of ERNP
Epinnula rex Ho, Motomura, Hata & Chiang
No full textEpinnula rex Ho, Motomura, Hata & Chiang, nomen novum urn:lsid:zoobank.org:act: 689988A0-823E-4EFC-85A8-2D485860BC3B Type series. As listed in Ho et al. (2017). Other materials. KAUM –I. 117161, 468.0 mm SL, Ishigaki Island, Yaeyama Islands, Okinawa Prefecture, Japan, 24 July 2018, coll. H. Hirasaka; KAUM –I. 141344, 820.0 mm SL, Ishigaki Island, 630–660 m depth, 5 Mar. 2017, H. Hirasaka; KAUM –I. 164522, 190.2 mm SL, Kumano-nada, off Kiinagashima, Kihoku, Mie Prefecture, Japan, 34°10′53″N, 136°21′16″E, 15 Dec. 2015; KAUM –I. 165071, 194.2 mm SL, Enshu-nada, off Omaezaki, Aichi Prefecture, Japan, 34°22′N, 138°11′E, 25 Dec. 2020, FRV Daihachi-tokai-maru; NMMB-P 20645, 285 mm SL, Daxi, Yilan, 22 Oct. 2011, coll. H.-C. Ho. NMMB-P 34200, 471 mm SL, Keelung fish market, 26 May 2020, coll. C.N. Tang; NSMT-P 143547, 736.9 mm SL, Ishigaki Island, 9 Dec. 2021, coll. H. Hirasaka. Etymology. The specific name means “king” in Latin, used as a noun, as an homage to its Atlantic congener Epinnula magistralis which was named for “master” in Latin. Remarks. Although being treated as a subspecies of what is now recognized as Neoepinnula orientalis, Grey (1953) pointed out the differences between E. o. pacifica (from Japan) and E. o. orientalis (from South Africa), which may be recognized as two different species after further study. The availability of Epinnula o. pacifica (now under Neoepinnula) requires that the 2017 E. pacifica have an amended name under the principle of homonymy. As such, a replacement name is provided. The new replacement name will take the same type series, as well as the same authorship with one change. For this nomen novum, we would like to correct the name of the fourth author (WCC) whose surname should be changed to Chiang.Published as part of Ho, Hsuan-Ching, Motomura, Hiroyuki, Hata, Harutaka & Chiang, Wei-Chuan, 2022, Epinnula rex nom. nov., a replacement name for Epinnula pacifica Ho, Motomura Hata & Jiang, 2017 (Teleostei: Gempylidae), pp. 199-200 in Zootaxa 5141 (2) on page 199, DOI: 10.11646/zootaxa.5141.2.8, http://zenodo.org/record/658172
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