226 research outputs found
Fucoxanthin Enhances HO-1 and NQO1 Expression in Murine Hepatic BNL CL.2 Cells through Activation of the Nrf2/ARE System Partially by Its Pro-oxidant Activity
No full textTo determine whether fucoxanthin, a major carotenoid in brown sea algae, may activate cellular antioxidant enzymes via up-regulation of the Nrf2/antioxidant-response element (ARE) pathway, we incubated mouse hepatic BNL CL.2 cells with fucoxanthin (0.5-20 mu M) for 0-24 h. We found that fucoxanthin (>= 5 mu M) significantly increased cellular reactive oxygen species (ROS) at 6 h of incubation, whereas preincubation with alpha-d-tocopherol (30 mu M) significantly attenuated the increase of ROS, indicating the pro-oxidant nature of fucoxanthin. Fucoxanthin significantly increased the phosphorylation of ERK and p38 and markedly increased nuclear Nrf2 protein accumulation after incubation for 12 h. Moreover, fucoxanthin significantly enhanced binding activities of nuclear Nrf2 with ARE and increased mRNA and protein expression of HO-1 and NQO1 after incubation for 12 h. siRNA inhibition of Nrf2 led to markedly decreased HO-1 and NQO1 protein expression. Thus, fucoxanthin may exert its antioxidant activity, at least partly, through its pro-oxidant actions
Preparation and characterization of cobalt-containing alcohols and diols
No full textA series of cobalt-containing alcohols and diols were prepared and characterized. Intramolecular hydrogen-bonding was observed for the cobalt-containing diols [COI(CO)6( mu-eta-(HO)R1R2CC CCR1,R,(OH)] (1: R, = CH3, R-2 = C2H5; 2: R, = CH3, R, = C(3)HA [CO'-(CO)6( mu-eta-(HO)Ph-2,CC CCPh (OH)] (3) and [(mu-PPh2CH h 2 - 2PP I)CO2(CO)4(P-yl-(HO)ph2CC CCPh,(OH)] (4). Potentially all the four compounds could serve as chelating 0,O-ligands. In principle, it is possible for compounds [(p-PPh,NHPPh1)CO2(CO)(4)(mu-eta-HC=-CCPh2,OH)] (5b), [CO2(CO)6(mu-eta-HC CC2H4OH] (6) and [Co2(CO)6(mu-eta-HC CC3H6OH)] (7) in their syn-conformations to behave as chelating 0,N-ligands. To the best of our knowledge, compounds 5b, 6 and 7 are the first reported examples of PPh2,NHPPh2,-bridged dicobalt complexes. (c) 2007 Elsevier Ltd. All rights reserved
Knowledge assessment is key to identifying the training needs of delirium care
No full textlink_to_subscribed_fulltex
Can financial incentives as a health policy strategy enhance clinical patient outcomes and engage evidence-based practice implementation?
No full textStrategies to Improve the Knowledge and Clinical Skills for Recognition and Management of Delirium of Critical Care Nurses
No full textDelirium is common among hospitalised individuals, particularly in intensive care units (ICUs). The complications of delirium among critically ill patients can lead to adverse outcomes. Critical care nurses are the frontline professionals providing direct care to critically ill patients. However, critical care nurses in Taiwan did not have adequate training or perform routine delirium assessment in their daily practice. Low awareness of delirium among critical care nurses contributes to a high incidence rate of ICU delirium. Therefore, the overall aim of this doctoral thesis was to develop an ICU-specific delirium care education programme to enhance the knowledge, confidence, competence and clinical performance of critical care nurses in Taiwan
Synthesis and structures of TiCl2(2-OC6H4OCH3)(2) and TiCl2(2-OC6H4OCH3)(mu-Cl) (2) and stepwise reactions of TiCl2(2-OC6H4OCH3)(mu-Cl) (2) with 2-propanol
No full textReaction of TiCl4 with 2 or 1 molar equiv. of 2-methoxyphenol gives the complex TiCl2(2-OC6H4OCH3)(2) (1) or [TiCl2(2-OC6H4OCH3)(mu-Cl)](2) (2), respectively. Complex 2 further reacts stepwise with 1 to 2 molar equiv, of 2-propanol to give the complexes [TiCl(O-i-Pr)(2-OC6H4OCH3)(mu-Cl)](2) (3) and TiCl2(O-i-Pr)(HO-i-Pr)(2-OC6H4OCH3) (4). The solid state structures of complexes 1 and 2 were analyzed by X-ray diffraction studies. Complexes 2, 3, and 4 are dynamic in solution. The variable-temperature H-1 NMR study of complex 2 reveals the presence of two major species in solution of relative abundance 1:2 in toluene-d(8). However, for complex 3, which differs from complex 2 in the replacement of one Cl- ligand on both Ti moieties by one 2-propoxide ligand, three major species along with several minor isomers are seen in solution at low temperatures. (C) 1999 Elsevier Science S.A. All rights reserved
Apo-8 '-lycopenal Induces Expression of HO-1 and NQO-1 via the ERK/p38-Nrf2-ARE Pathway in Human HepG2 Cells
No full textLycopene and its metabolite apo-10'-lycopenoic acid have been shown to induce phase II detoxifying/antioxidant enzymes through activation of the nuclear factor erythroid-derived 2-like 2 (Nrf2)-antioxidant response element (ARE) transcription system. However, little is known about whether apo-8'-lyocpenal, one of the main metabolites of lycopene in rat livers, in lycopene-containing food, and in human plasma, has similar effects. This study investigated the effect of apo-8'-lycopenal on Nrf2-ARE system mediated heme oxygenase 1 (HO-1) and NAD(P)H:quinine oxidoreductase 1 (NQO-1) expression in human HepG2 cells. It was found that apo-8'-lycopenal (1-10 mu M) significantly increased nuclear Nrf2 accumulation, ARE-luciferase activity, Nrf2-ARE binding activity, chymotrypsin-like activity, and downstream HO-1 and NQO-1 expression, but decreased cytosolic Ketch-like ECH-associated protein 1 (Keap1) expression. Results also revealed that the ERK/p38-Nrf2 pathway is involved in activation of HO-1 and NQO-1 expression by apo-8'-lycopenal using Nrf2 siRNA and ERK/p38 specific inhibitors. In addition, the activation time of lycopene on nuclear Nrf2 accumulation. is slower than that of apo-8'-lycopenal, suggesting that the chemopreventive effects of lycopene may be partially attributed to its metabolites
Application of the delirium risk prediction model in the TED ICU smart intensive care system during the current COVID-19 pandemic
No full textlink_to_OA_fulltex
- …
