885 research outputs found
La cavale de Tony Racaille / François Bayot ; illustration de couverture Samir Hmida
No full textCollection : Souris poche ; 22Appartient à l’ensemble documentaire : UnivJeun0Contient une table des matièresAvec mode text
Database paper HMIDA&REY(2023)
No full textThis dataset was used to study the effect of energy access, traditional and modern renewable energy consumption on human development for a panel of 44 emerging and developing countries, for the period 1990-2018. All data are from UNDP, WDI, SE4ALL and Bp databases.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV
Overexpression of a Bacillus subilis amylase in E.coli and application in bread making
No full textBacterial alpha-amylases (EC3.2.1.1) have potential use in wide number of industrial applications such as textile, paper, detergent, food, fermentation and pharmaceutical industries. Recombinant DNA technology for amylase production involves the selection of an amylase gene, its insertion into an appropriate vector system, transformation in an efficient bacterial system to produce high amount of recombinant protein. In this context, the aim of this work is the overexpression of an α-amylase gene from Bacillus subtilis US572 in E.coli strain, the characterization of the recombinant enzyme and test the effect of different quantities added of amylase on wheat flours and bread characterization
Structure/function/properties relationships and application of a GH11 xylanase
No full textXylanases are hemicellulolytic enzymes, which are responsible for the degradation of the heteroxylans constituting the lignocellulosic plant cell wall. Due to their variety, xylanases have been classified in glycoside hydrolase families GH5, GH8, GH10, GH11, GH30 and GH43 in the CAZy database. In this work, we focus on GH11 family, which is one of the best characterized GH families with bacterial and fungal members. GH11 xylanases have for a long time been used as biotechnological tools in various industrial applications and represent in addition promising candidates for future other uses
Low Power Instrumentation Amplifier for a Fully Implantable Neural Recording System
No full textRecording neural signal from a living human body is a complex
task and it is an important research issues for neuroscientists and
researchers in biomedical engineering. The major issue to over-
come in the design of a system that is aimed at being implant
into the human body is having a low power consumption, low
noise circuit and small dimension to minimize tissue damage. In
this paper, specific issues of the most important part of such a
neural acquisition system are presented; in particular, the design
of a low-power amplifier, for a fully implantable neural recording
system, is described. The amplifier uses a differential pair as
input stage. Given that neural amplifiers must include differen-
tial input pair to achieve a high common-mode ratio rejection
(CMRR). The amplifier has been designed in the AMS 0.35 μm
standard CMOS process. The amplifier current consumption is
4.61 μA at ±1V supply, which gives a power consumption of
9.22 μW. The low cutoff frequency is adjustable from 21 Hz to
100 Hz, with four tunable gains of 43.6 dB, 48 dB, 50 dB and 52.8
dB. The upper cutoff frequency is about 7.6 kHz. The CMRR is
113 dB and the power supply ratio rejection is PSRR > 73dB.
The input referred noise is 14.8 μV rms over 100 10 kHz. The
amplifier gives an input DC offset of 196 μV
Aroma volatile components, fatty acids and antibacterial activity of four Tunisian Punica granatum L. flower cultivars
No full textThis study investigates the varietal effects on volatile components and fatty acids composition (analysed by GC–MS and GC, respectively) as well as the antibacterial propriety (using microdilution test) from four Tunisian pomegranate flower cultivars (Tounsi, Nabli, Gabsi and Chelfi). The Chelfi flowers seemed to be the richest in terpenoids (28.39%), aldehydes (13.24%) and alcohols (22.30%). Those volatiles seemed to be the most important groups with apparent difference between varieties. Fatty acid profiles varied significantly among cultivars. The most abundant saturated fatty acid was palmitic acid where Nabli cultivar exhibited the highest amount (435.80 μg/g). However, Gabsi cultivar had the highest content of unsaturated fatty acids where the linolenic acid and linoleic acid were the most frequent polyunsaturated fatty acids with 254.42 and 707.73 μg/g, respectively. Results presented here may suggest that the volatiles fraction of the studied cultivars possess antimicrobial activities. This work gives further knowledge for extensive development of this medicinal plan
Biochemical characterization and structural insights into the high substrate affinity of a dimeric and Ca2+ independent Bacillus subtilis α-amylase
No full textAn extracellular amylase (AmyKS) produced by a newly isolated Bacillus subtilis strain US572 was purified and characterized. AmyKS showed maximal activity at pH 6 and 60 °C with a half-life of 10 min at 70 °C. It is a Ca2+ independent enzyme and able to hydrolyze soluble starch into oligosaccharides consisting mainly of maltose and maltotriose. When compared to the studied α-amylases, AmyKS presents a high affinity towards soluble starch with a Km value of 0.252 mg mL-1 . Coupled with the size-exclusion chromatography data, MALDI-TOF/MS analysis indicated that the purified amylase is a dimer with a molecular mass of 136,938.18 Da. It is an unusual feature of a non-maltogenic α-amylase. A 3D model and a dimeric model of AmyKS were generated showing the presence of an additional domain suspected to be involved in the dimerization process. This dimer arrangement could explain the high substrate affinity and catalytic efficiency of this enzyme
Biochemical and molecular characterization of a recombinant α-amylase from Bacillus subtilis
No full textAlpha amylase (EC3.2.1.1), one of more widespread enzymes in the industrial world, is a glycoside hydrolase. It hydrolyzes the α- (1,4) glucoside linkage between the glucose units of a polysaccharide. Microbial amylases, particularly those from Bacillus genus are more in demand than those from other sources. Alpha-amylases have potential application in wide number of industrial applications such as textile, paper, detergent, food, fermentation and pharmaceutical industries. Recombinant DNA technology for amylase production involves the selection of an efficient amylase gene, its insertion into an appropriate vector system, transformation in an efficient bacterial system to produce high amount of recombinant protein. In this context, the aim of this work is the overexpression of anαamylase gene from Bacillus subtilisUS572in E. coli strain and the characterization of the recombinant amylase which is an interesting candidate for biotechnological applications
Extracting Electric Power From Human Body For Supplying Neural Recording System
No full textA powerful approach to the characterization of cellular electrical activity is electrical recording from cells or living tissues. The human central and / or peripheral nervous system has been a subject of study and fascination of the neuroscience and biomedical engineering communities for many decades. In this paper, we propose a new approach to feed implantable neural recording system, which based on extracting electrical power from human tissue warmth in order to supply a biomedical neural recording system. The major issue to overcome, in the design of a system that is aimed at being implant into the human body, is having a low power consumption, low noise circuit and small dimension to minimize tissue damage
Low Power Instrumentation Amplifier for a Fully Implantable Neural Recording System
No full textRecording neural signal from a living human body is a complex
task and it is an important research issues for neuroscientists and
researchers in biomedical engineering. The major issue to over-
come in the design of a system that is aimed at being implant
into the human body is having a low power consumption, low
noise circuit and small dimension to minimize tissue damage. In
this paper, specific issues of the most important part of such a
neural acquisition system are presented; in particular, the design
of a low-power amplifier, for a fully implantable neural recording
system, is described. The amplifier uses a differential pair as
input stage. Given that neural amplifiers must include differen-
tial input pair to achieve a high common-mode ratio rejection
(CMRR).
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