1,720,953 research outputs found
Multiscale Sensing of the Physical Cellular Environment: Phase-field Modeling and Experiments
No full textCells constantly interact with their physical environment by sensing and responding to mechanical and topographical cues. These cues span multiple scales, from subcellular interactions with the extracellular matrix to population-scale confinement in morphogenesis. Central to this process is the actin cytoskeleton, which serves as both a local force generator and a medium for signal integration and propagation. In this dissertation, I combine multiscale computational modeling with quantitative imaging to investigate how actin dynamics drive physical sensing from the scale of single protrusions to collective cellular behavior.
The actin cytoskeleton is the primary mechanism for generating forces that cause cell protrusions and guided migration. Using 12Z cells as a model of endometriosis, we examine how exposure to the biochemical signal estradiol alters actin organization and, consequently, cell morphology. High-resolution 3D imaging reveals that estradiol treatment increases protrusion size and disorder in actin dynamics, consistent with enhanced cellular invasiveness. These findings highlight how chemical signals modulate mechanical output through actin-based protrusions, reinforcing the role of actin as a key transducer of biochemical cues into physical motion.
At the subcellular scale, we use a 3D phase-field model to illustrate how cells exhibit unidirectional migration on asymmetric nanotopographies, with directionality controlled by actin polymerization rate and topographic scale. In this model, an asymmetric substrate alone can cause spontaneous polarization and reproduce the shape and guidance morphologies observed experimentally. These predictions align with a reanalysis of \textit{D. discoideum} experiments, revealing that guidance on subcellular sawteeth depends on both cell velocity and feature height. This agreement indicates that membrane deformation and local curvature sensing, driven by actin forces, are sufficient to bias migration in complex microenvironments.
To study how the actin cytoskeleton responds to chemical cues found in the extracellular matrix, we analyze epithelial cell migration on collagen-coated nanoridges. On nanoridges, collagen IV enhances actin alignment and cell elongation; however, actin guidance remains decoupled from the direction of migration. Therefore, additional mechanisms, such as focal adhesion dynamics, may contribute to directional sensing.
At larger scales, we develop a scalable 2D multicellular phase-field model incorporating excitable actin dynamics. This framework enables simulations of thousands of deformable cells on consumer hardware. The model spans a range of scales, allowing cell interaction, long-distance wave propagation, and information exchange. In this model, excitable intracellular mechanics, along with local physical interactions, can lead to emergent synchronization and local sensing of the shape of large-scale confinement.
Together, these findings suggest that actin serves as a mechanochemical interface for multiscale environmental sensing by driving local protrusions, integrating physical signals, and enabling collective coordination through excitable dynamics. Actin polymerization additionally acts as an upstream regulator of other cell functions, which opens avenues for future experimental studies on the effects of actin synchronization and pulsing on biophysical behavior. By linking cytoskeletal signaling to large-scale coordination, this work lays the foundation for identifying new physical mechanisms underlying biological processes that require coordination, such as metastasis and tissue morphogenesis
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
Author Under Sail The Imagination of Jack London, 1893-1902
No full textIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Intro -- Title Page -- Copyright Page -- Dedication -- Contents -- Acknowledgments -- Introduction -- 1. Spirit Truth -- 2. From Absorption to Theatricality and Back Again -- 3. "I Will Build a New Present" -- 4. Sons as Authors -- 5. Fathers as Publishers -- 6. The Daughter as Author -- 7. Lovers as Authors -- 8. At Sea with the Family -- 9. Yellow News, Yellow Stories -- 10. The Return Home -- Notes -- Bibliography -- Index -- About Jay WilliamsIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
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