162,676 research outputs found
Can Union support reduce the negative effects of job insecurity on well-being?
No full textIn the context of rapidly changing environmental conditions, innumerable organizations engage in restructuring activities. As a consequence, many employees feel insecure about the future existence of their jobs. While research suggests that such job insecurity has negative consequences for employee attitudes and well-being, less is known about if and how these negative effects can be alleviated by social support from the union. This present study tests for a potential moderator effect of perceived union support in the insecurity-mental health complaints relation using survey data collected among unionized workers in Italy, the Netherlands and Sweden. The results indicate that job insecurity is associated with mental health complaints in all participating countries and that union support relates negatively to mental health complaints in one of the participating countries. However, no interaction effect was obtained, thus indicating that union support does not reduce the effects of job insecurity on mental health complaints
[Report to Chief J. E. Curry, by an unknown author #1]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
[Report to Chief J. E. Curry, by an unknown author #2]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
Modelling self-sustained rhythmic activity in lamprey hemisegmental networks
No full textRecent studies of the lamprey spinal cord have shown that hemisegmental preparations can display rhythmic activity in response to a constant input drive. This activity is believed to be generated by a network of recurrently connected excitatory interneurons. A recent study found and characterized self-sustaining rhythmic activity—locomotor bouts—after brief electrical stimulation of hemisegmental preparations. The mechanisms behind the bouts are still unclear. We have developed a computational model of the hemisegmental network. The model addresses the possible involvement of NMDA, AMPA, acetylcholine, and metabotropic glutamate receptors as well as axonal delays in locomotor bouts
Visual function in very low birth weight adolescents : fifteen-year follow-up of children in southeast Sweden
No full textBackground: Very low birth weight (VLBW Aims: To describe visual functions in adolescents with VLBW in comparison with a matched control group and to investigate associations with white matter damage of immaturity (WMDI), optic disc measurements and cognitive functions in the VLBW group.Subjects (Papers I-III): A total of 86 VLBW children survived the neonatal period during a 15 months period in the southeast region of Sweden. Fifty-nine of those, and 55 term control infants, participated in the 15-year follow-up study. (Paper IV): A subgroup including 18 VLBW subjects and 29 control subjects participated.Methods: Structural assessments included brain MRI, digital analysis of fundus photographs and cycloplegic refraction. Functional evaluations comprised best corrected visual acuity, stereo acuity, visual fields, ocular alignment, fixation behavior, cognitive visual problems and intellectual level.Results: Twenty-eight percent of the VLBW subjects had WMDI. The mean neural retinal rim area was smaller - in normal sized optic discs - in the VLBW than in the control group (p=0.018). The VLBW adolescents had more tortuous retinal arterioles than the controls (pConclusion: This study confirms previous observations that adolescents with VLBW are at a disadvantage regarding visual and cognitive outcome compared with adolescents with normal birth weight. Adolescents with WMDI had more pronounced visual and cognitive dysfunction.List of scientific papersI. Hellgren K, Hellström A, Jacobson L, Flodmark O, Wadsby M, Martin L (2007). "Visual and cerebral sequelae of very low birth weight in adolescents." Arch Dis Child Fetal Neonatal Ed 92(4): F259-64. https://pubmed.ncbi.nlm.nih.gov/17314116 II. Hellgren K, Hellström A, Martin L (2008). "Visual fields and optic disc morphology in very low birthweight adolescents examined with magnetic resonance imaging of the brain." Acta Ophthalmol Sep 20: Epub ahead of print. https://pubmed.ncbi.nlm.nih.gov/18811637 III. Hellgren K, Aring E, Jacobson L, Ygge J, Martin L (2008). "Visuo-spatial skills, ocular alignment and MRI findings in very low birth weight adolescents." Journal of American Association for Pediatric Ophthalmology and Strabismus. [Accepted] https://doi.org/10.1016/j.jaapos.2008.11.008 IV. Hellgren K, Han Y, Ygge J (2009). "Fixation behaviour in very low birth weight and control adolescents." [Manuscript]</p
Enzymatic studies of alcohol dehydrogenase by a combination of in vitro and in silico methods
No full textThe family of alcohol dehydrogenases (ADHs) catalyzes conversions of alcohols, ketons and aldehydes. The early discovery and isolation of ADH (1937) was followed by numerous investigations. It was also the first dimeric enzyme for which the threedimensional structure was determined (1974). Recent findings have revealed new physiological functions for the ADH enzymes. One type is a key enzyme in hepatic retinol metabolism, another is a main formaldehyde scavenger and a regulator of Snitrosothiols levels. ADH genes have been shown to be connected to diseases and syndromes, such as alcoholism and asthma. Hence, investigations of structure-function relationships of the ADH enzymes are of both physiological and medical interest. The aim of this thesis was to study structure-function relationships and to investigate and identify ligands for ADH, with in vitro and in silico methods.The catalytic activities of human, mouse and rat ADH2 for retinoids, were determined. The Km values for human ADH2 are the lowest among all known human dehydrogenases, which supports a key role for human ADH2 in the hepatic retinoid metabolism. ADH3 is an enzyme with a proposed role as an NO scavenger. Two new lines of ligands, bile acids and fatty acids, were investigated for their potential effects on NO homeostasis. The bronco dilatatory effect of NO suggests that ADH3 inhibition could potentially work as treatment of obstructive lung disorders. The stability of the quaternary structure of sorbitol dehydrogenase (SDH) was determined by in vitro experiments and in silico energy calculations. A hydrogen-bonding network crucial for the tetrameric stability in SDH was identified. This network is located at a region enclosing the structural zinc site in mammalian ADHs.The structural zinc site was studied in detail by a combination of molecular dynamics and quantum mechanics simulations. The simulations revealed that the interaction between the cysteine residues and the zinc atom is of an electrostatic and covalent nature. With in silico and in vitro simulations, interactions between ligands and the active site were determined, revealing site specific interactions within both ADH2 and ADH3. Furthermore, studies of subunit interactions and the structural zinc site revealed properties of the quaternary stability.List of scientific papersI. Hellgren M, Strömberg P, Gallego O, Martras S, Farrés J, Persson B, Parés X, Höög JO (2007). Alcohol dehydrogenase 2 is a major hepatic enzyme for human retinol metabolism. Cell Mol Life Sci. 64(4): 498-505 https://pubmed.ncbi.nlm.nih.gov/17279314II. Hellgren M, Kaiser C, de Haij S, Norberg A, Höög JO (2007). A hydrogen-bonding network in mammalian sorbitol dehydrogenase stabilizes the tetrameric state and is essential for the catalytic power. Cell Mol Life Sci. 64(23): 3129-38 https://pubmed.ncbi.nlm.nih.gov/17952367III. Brandt EG, Hellgren M, Brinck T, Bergman T, Edholm O (2009). Molecular dynamics study of zinc binding to cysteines in a peptide mimic of the alcohol dehydrogenase structural zinc site. Phys Chem Chem Phys. 11(6): 975-83. Epub 2008 Dec 12 https://pubmed.ncbi.nlm.nih.gov/19177216IV. Staab CA, Hellgren M, Grafström RC, Höög JO (2009). Medium-chain fatty acids and glutathione derivatives as inhibitors of S-nitrosoglutathione reduction mediated by alcohol dehydrogenase 3. Chem Biol Interact. 180(1): 113-8. Epub 2009 Jan 29 https://pubmed.ncbi.nlm.nih.gov/19428350V. Hellgren M, Carlsson J, Östberg L, Staab C.A, Persson B, Höög JO (2009). Virtual screening for ligands to human alcohol dehydrogenase 3. [Manuscript]</p
Murder on the mountain: author talk with Peter J. Wosh
No full textAuthor talk by Peter J. Wosh on May 5th, 2022, on his book, "Murder on the Mountain: crime, passion, and punishment in gilded age New Jersey.
Mr. Melvin J. Collier, RWWL AUC, June 2011
No full textThis video is a conversation with Mr. Melvin J. Collier. Mr. Collier talks about his book, "From Mississippi to Africa: A Journey of Discovery". Daniel Le, AUC Woodruff Library, is the interviewer
A single Markov-type kinetic model accounting for the macroscopic currents of all human voltage-gated sodium channel isoforms.
No full textModelling ionic channels represents a fundamental step towards developing biologically detailed neuron models. Until recently, the voltage-gated ion channels have been mainly modelled according to the formalism introduced by the seminal works of Hodgkin and Huxley (HH). However, following the continuing achievements in the biophysical and molecular comprehension of these pore-forming transmembrane proteins, the HH formalism turned out to carry limitations and inconsistencies in reproducing the ion-channels electrophysiological behaviour. At the same time, Markov-type kinetic models have been increasingly proven to successfully replicate both the electrophysiological and biophysical features of different ion channels. However, in order to model even the finest non-conducting molecular conformational change, they are often equipped with a considerable number of states and related transitions, which make them computationally heavy and less suitable for implementation in conductance-based neurons and large networks of those. In this purely modelling study we develop a Markov-type kinetic model for all human voltage-gated sodium channels (VGSCs). The model framework is detailed, unifying (i.e., it accounts for all ion-channel isoforms) and computationally efficient (i.e. with a minimal set of states and transitions). The electrophysiological data to be modelled are gathered from previously published studies on whole-cell patch-clamp experiments in mammalian cell lines heterologously expressing the human VGSC subtypes (from NaV1.1 to NaV1.9). By adopting a minimum sequence of states, and using the same state diagram for all the distinct isoforms, the model ensures the lightest computational load when used in neuron models and neural networks of increasing complexity. The transitions between the states are described by original ordinary differential equations, which represent the rate of the state transitions as a function of voltage (i.e., membrane potential). The kinetic model, developed in the NEURON simulation environment, appears to be the simplest and most parsimonious way for a detailed phenomenological description of the human VGSCs electrophysiological behaviour
A Tripartite Post-Recession Rebalancing
No full textIn this latest Advance & Rutgers Report, entitled “A Tripartite Post-Recession Rebalancing,” Dean James W. Hughes and Professor Joseph J. Seneca deliver an incisive assessment of the current market conditions and obstacles in the path of our economic recovery. They offer a statistical cautionary tale that the private and public sector need to hear and acknowledge in order for the economy to make continued progress.This report was published as Issue Paper Number 7, November 2011, in Advance & Rutgers Report
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