172,830 research outputs found

    Heidemann, C.

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    High resolution Rutherford backscattering spectroscopy studies on Mo/Si multilayers

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    Heidemann B, Tappe T, Schmiedeskamp B, Heinzmann U. High resolution Rutherford backscattering spectroscopy studies on Mo/Si multilayers. Thin Solid Films. 1993;228(1-2):60-63.High resolution Rutherford backscattering spectroscopy with an electrostatic analysis of the ion energy is applied to Mo/Si multilayers with a period of 7 nm. The multilayers have been produced for X-ray optical purposes by electron beam evaporation in ultrahigh vacuum at three different temperatures during deposition: 30, 150 and 200 °C. In the Rutherford backscattering spectra the layer structure is resolved in all three cases. The multilayers deposited at 150 and 200 °C show interlayers of mixed Mo and Si of different thicknesses on the two sides of a Mo layer. The most distinct layer structure is found for the 150 °C sample, whereas the spectra for the 30 °C sample indicate a larger interfacial roughness and those for the 200 °C sample larger interfacial layers of mixed mo and Si than for the 150 °C sample. On baking the multilayers to temperatures higher than 400 °C, interdiffusion of Mo and Si is observed. The multilayers deposited at 150 and 200 °C are destroyed after baking to 600 °C, whereas the multilayer deposited at 30 °C has already been destroyed after baking to 500 °C. Up to a baking temperature of 600 °C neither losses of material from the stack nor accumulation of Mo or Si at the surface or the interface between the stack and substrate are observed

    Dissertatio Ivridica De Conatv Delinqvendi

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    Halle, Univ., Jur. Diss., 1799Qvam Avctoritate Illvstris Ivreconsvltorvm Ordinis Ad Obtinendvm Doctoris Gradvm Die VIII Maii MDCCXCIX Pvblice Defendet Avctor Avgvstvs Gvilielmvs Heidemann, Stargardia-PommeranvsVorlageform des Erscheinungsvermerks: Halae, Typis I. C. Hendelianis

    The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator

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    Heidemann R, Riese U, Lütkemeyer D, Büntemeyer H, Lehmann J. The Super-Spinner: a low cost animal cell culture bioreactor for the CO 2 incubator. Cytotechnology. 1994;14(1):1-9.The production of small quantities of monoclonal antibodies and recombinant proteins was carried out using a new low cost production system, the Super Spinner. Into a 1 1 standard Duran(R) flask a membrane stirrer equipped with a polypropylene hollow fiber membrane was installed to improve the oxygen supply by bubble-free aeration. The aeration was facilitated by using the CO2 conditioned incubator gas, which was pumped through the membrane stirrer via a small membrane pump. The maximal oxygen transfer rate (OTR(max)) of the Super Spinner was detected. For this purpose one spinner flask was equipped with an oxygen electrode. The OTR(max) was measured by the dynamic method. The ratio of membrane length to culture volume was adapted corresponding to the oxygen uptake rate of the cells according to the desired cell density. A balanced nutrient supply resulted in an optimal formation and yield of products

    Crystal structures of the c-di-AMP–synthesizing enzyme CdaA

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    Cyclic di-AMP (c-di-AMP) is the only second messenger known to be essential for bacterial growth. It has been found mainly in Gram-positive bacteria, including pathogenic bacteria like Listeria monocytogenes. CdaA is the sole diadenylate cyclase in L. monocytogenes, making this enzyme an attractive target for the development of novel antibiotic compounds. Here we report crystal structures of CdaA from L. monocytogenes in the apo state, in the post-catalytic state with bound c-di-AMP and catalytic Co2+ ions, as well as in a complex with AMP. These structures reveal the flexibility of a tyrosine side chain involved in locking the adenine ring after ATP binding. The essential role of this tyrosine was confirmed by mutation to Ala, leading to drastic loss of enzymatic activity

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Fallkonferenz: Hochbegabung in Kindergarten und Grundschule

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    Wegner C, Völker-Meier P, Heidemann M, Hartmann C, Remmert K. Fallkonferenz: Hochbegabung in Kindergarten und Grundschule. Labyrinth. 2013;117(8):42-43
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