436 research outputs found
Advances in the Study of SR Protein Family
No full textThe name of SR proteins is derived from their typical RS domain that is rich in serine (Ser, S) and arginine (Arg, R). They are conserved in evolution. Up to now, 10 members of the SR protein family have been identified in humans. SR proteins contain one or two RNA binding motifs aside from the RS domain, and also possess special biochemical and immunological features. As to the functions of SR proteins, they facilitate the recruitment of the components of splicesome via protein-protein interaction to prompt the assembly of early splicesome; while in alternative splicing, tissue-specifically expressed SR protein along with the relative ratio of SR protein and heterogeneous nuclear ribonucleoprotein (hnRNP) is composed of two main regulative mechanisms for alternative splicing. Almost all of the biochemical functions are regulated by reversible phosphorylation
The 12th Human Liver Proteome Project (HLPP) Workshop, September 2010, Sydney, Australia
No full textATM-mediated NuSAP phosphorylation induces mitotic arrest
No full textNuSAP is a microtubule-associated protein that plays an important role in spindle assembly. NuSAP deficiency in mice leads to early embryonic lethality. Spindle assembly in NuSAP-deficient cells is highly inefficient and chromosomes remain dispersed in the mitotic cytoplasm. ATM is a key kinase that phosphorylates a series of substrates to mediate G1/S control. However, the role of ATM at the G2/M phase is not well understood. Here we demonstrate that ectopic expression of NuSAP lead to mitotic arrest observably dependent on the kinase activity of ATM. When endogenous ATM was depleted or its kinase activity was inhibited. NuSAP could not cause mitotic arrest. We further show ATM interacts with NuSAP and phosphorylates NuSAP on Ser124. The phosphorylation and interaction occur specifically at G2/M-phase. Collectively, our work has uncovered an ATM-dependent checkpoint pathway that prevents mitotic progression by targeting a microtubule-associated protein, NuSAP. (C) 2010 Elsevier Inc. All rights reserved.Biochemistry & Molecular BiologyBiophysicsSCI(E)5ARTICLE1413-41840
New trends in active filters for improving power quality
No full textSince their basic compensation principles were proposed around 1970, active filters have been studied by many researchers and engineers aiming to put them into practical applications. Shunt active filters for harmonic compensation with or without reactive power compensation, flicker compensation or voltage regulation have been put on a commercial base in Japan, and their rating or capacity has ranged from 50 kVA to 60 MVA at present. In near future, the term of active filters will cover a much wider sense than that of active filters in the 1970s did. The function of active filters will be expanded from voltage flicker compensation or voltage regulation into power quality improvement for power distribution systems as the capacity of active filters becomes larger. This paper describes present states of the active filters based on state-of-the-art power electronics technology, and their future prospects toward the 21st century, including the personal view and expectation of the author</p
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