1,721,005 research outputs found
Minor embedding with Stuart-Landau oscillator networks
No full textWe theoretically implement a strategy from quantum computation architectures to simulate Stuart-Landau oscillator dynamics in all-to-all connected networks, also referred to as complete graphs. The technique builds upon the triad structure minor embedding which expands dense graphs of interconnected elements into sparse ones which can potentially be realized in future onchip solid state technologies with tunable edge weights. As a case study, we reveal that the minor embedding procedure allows simulating the XY model on complete graphs, thus bypassing a severe geometric constraint
Construction and characterization of protein rLG, a novel 16.5 kDa hybrid protein with a large binding repertoire for immunoglobulin fragments
No full textSeveral proteins isolated from the surface of Gram-positive pathogenic bacteria have been shown to bind immunoglobulin (Ig) in a non-antigenic manner. The most widely studied of these proteins are protein A from Staphylococcus aureus, protein G from groups C and G streptococci, and protein L from Peptostreptococcus magnus. Although very useful reagents these all have limitations to their general applicability. Attempts have previously been made to broaden the binding spectra of individual Ig-binding molecules through the production of multidomain fusion proteins. The binding repertoires of proteins A and G restricts binding of a construct to specific Ig isotypes only. The ability of protein L to uniquely bind the variable domain of k-chains makes this protein a potential tool for the purification of Ig regardless of isotype. In the present study, a single k-chain binding domain of protein L was linked to a single Fc-binding domain of protein G to yield the novel, recombinant protein rLG gene. The rLG gene was cloned into the expression vector pKK223-3 allowing it to be over-expressed in E.coli JM103 cells. These studies show that despite its small size (Mr = 16.5 kDa), protein rLG exhibits simultaneous binding of ligands to both moieties, thereby making it a more versatile tool for Ig purification. A program of site directed mutagenesis (SDM) has been employed to characterize the binding properties and structural stability of protein rLG by equilibrium and stopped-flow fluorimetry, isothermal titration calorimetry, immuno-diffusion assay, affinity chromatography, circular dichroism and chemical denaturation studies
Studies on the binding interaction between peptostreptococcal protein L and a recombinant Fv
No full textProtein L is a multidomain cell wall protein from Peptostreptococcus magnus that is one of a group of proteins capable of binding to antibodies without producing an immune response. In contrast to other immunoglobulin (Ig) binding proteins, such as protein A from Staphylococcus aureus and protein G from group C and G streptococci, which bind at the CH2–CH3 interface, protein L binds exclusively to the VL domain of k-chains. It has previously been shown that a single Ig binding domain of protein L (PpL) has two sites of interaction with the VL domain, with the affinity of the second site up to 50-fold less than that of the first, depending on the nature of the k-chain. The two sites on PpL are distinct, with only one common residue implicated in both interactions. In contrast, the binding sites on the VL domain appear to have 10 residues common to both PpL interactions.The binding interaction between PpL and a 12.6 kDa variable light chain fragment (Fv) has been investigated. Production of a recombinant PpL and recombinant Fv fragment allows the structure of each species and the specific nature of the binding interaction to be examined using a range of techniques, including stopped-flow fluorimetry, circular dichrosism, isothermal titration calorimetry and NMR. To further understand the interaction between PpL and Fv, in particular at the second binding site, mutagenesis of both species has been undertaken and here we discuss the effect of those changes on the affinity of PpL for Fv and vice versa
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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