6 research outputs found
Kauppi-Niihaman käyttö opetuksessa
No full textTämän opinnäytetyön tarkoituksena oli selvittää, miten laajasti Kaupin vieressä sijaitseva Tampereen ammattikorkeakoulu käyttää aluetta osana eri koulutusohjelmien opetusta. Lisäksi mietittiin millä tavoin läheisten alakoulujen ensimmäisen ja toisen vuosiluokan opetuksessa voitaisiin hyödyntää aktiivisemmin vieressä sijaitsevaa Kauppi-Niihaman lähimetsää. Tutkimuksen aikana seurattiin myös Tampereen kaupungilla käynnissä olevaa Kauppi-Niihaman vertaissuunnitteluprosessia. Ryhmien tekemistä suunnitteluratkaisuista poimittiin ideoita mukaan opinnäytetyöhön. Työn tilaajana oli Tampereen ammattikorkeakoulun metsätalouden koulutusohjelma. Tutkimukseen haastateltiin niitä koulutusohjelmia, joilla oli opetustoimintaa Kauppi-Niihaman alueella.
Peruskoulun opetussuunnitelmassa (2014) painotetaan luonnonympäristöjen tärkeyttä oppimisympäristöinä. Luonto nähdään merkittävänä tekijänä nuorten hyvinvoinnin edistämiseksi. Kauppi-Niihaman sijainti lähellä useita alakouluja mahdollistaa sen opetuskäytön monissa eri oppiaineissa. Alueen monimuotoiset metsät tarjoavat paikan luontoretkeilyille ja mahdollisuuden siirtää opetusta luokasta maastoon. Rakentamattomien ympäristöjen käyttö alakoulujen opetuksessa luo perustan nuoren ympäristökasvatukselle.
Tampereen ammattikorkeakoulun koulutusohjelmat, joilla oli opetusta Kauppi-Niihamassa mielsivät alueen tärkeäksi opetusta elävöittäväksi tekijäksi. Moniaistinen, kokonaisvaltainen ja suora havainnointi antaa opiskelijoille omakohtaisia kokemuksia. Suurimmat haasteet maasto-opetuksen toteuttamiseen liittyivät kauemmaksi Niihamaan kulkemiseen sekä opetuksen resurssien vähenemiseen. Kauppi-Niihaman alueen käytön uskottiin kuitenkin säilyvän tai jopa lisääntyvän koulutusohjelmien opetuksessa.
Kauppi-Niihaman alue on kehittynyt valtavasti Kaupin kansanpuiston ja Niihaman entisten viljelysmaiden ajoilta. Se on tärkeä monille paikallisille ja tällä hetkellä sitä halutaankin kehittää yhdessä kaupunkilaisten kanssa, vertaissuunnitelua hyödyntäen. Aluetta koskevat muutospaineet myös tulevaisuudessa ja siksi onkin tärkeää ratkaista alueen käytön erimielisyydet yhdessä.Kauppi-Niihama Forest is a so called city forest situated near the centre of Tampere and bordering with Lake Näsijärvi. The purpose of this research was to find out how much Tampere University of Applied Sciences next to Kauppi is using the area as part of the teaching in various educational programs. In addition, it was considered how the nearby forest could be used more actively in the first- and second-year students teaching in primary schools. During the research the ongoing planning process of the forest was also followed. It is run by the city of Tampere and ideas from there were included to this thesis. The research was commissioned by the Degree Programme in Forestry of Tampere University of Applied Sciences. Members of other degree programs that had activities in the area were interviewed for the study.
Degree programs which were teaching in Kauppi-Niihama considered the area an important education factor. Multisensory, comprehensive and direct observation gives personal experiences to students. The biggest challenges to execute field work involves moving further to Niihama and also the lower education resources. The use of KauppiNiihama area was believed to remain or even increase in teaching of education programs.
The comprehensive school student-oriented teaching methods (2014) emphasizes the importance of natural environments as learning environments. Nature is seen as an important factor in promoting the well-being of young people. The location of Kauppi-Niihama close to several primary schools makes it possible to use it in the teaching of many subjects. All kind of forests in the area offer a place for nature tours and opportunity to move lessons from class to forest. By using natural and unbuilt environments in primary school education gives the basis for children’s environmental education.
The area of Kauppi-Niihama has developed enormously. It is important to many locals and now that want to develop together with the inhabitants by using the peer planning. The area concerns a lot of future pressures and that is why it is important to solve disagreements about the use of area together
Dose influence on the PMMA e-resist for the development of high-aspect ratio and reproducible sub-micrometric structures by electron beam lithography
No full textIn this work, a statistical process control method is presented showing the accuracy and the reliability obtained with of PMMA E-resist AR-P 672, using an Elphy Quantum Electron Beam Lithography module integrated on a FE-SEM Zeiss Auriga instrument. Reproducible nanostructures with an high aspect ratio between e-resist thickness and width of written geometric structure are shown. Detailed investigation of geometry features are investigated with dimension in the range of 200nm to 1-m. The adopted method will show how tuning the Area Dose factor and the PMMA thickness it was possible to determine the correct and reproducible parameters that allows to obtain well defined electron-beam features with a 4:1 aspect ratio. Such high aspect ratio opens the possibility to realize an electron-beam lithography lift-off process by using a standard e-beam resist. © 2016 Author(s)
Fungal planktonic community related to salinity and temperature in an oligotrophic sea.
No full textMarine fungi play a crucial role in carbon cycling and food webs by acting as saprophytes or parasites and shaping host communities. However, our knowledge of these fungi in the marine ecosystem remains limited. To address this gap, we conducted a study to investigate the diversity of planktonic fungal communities in the Red Sea, a warm and oligotrophic sea. We collected water samples from the photic layer at six sites along the Red Sea basin and analyzed the fungal community by targeting the 28S rRNA gene. Our results showed that Chytridiomycota dominated these communities, accounting for 85% of reads, followed by members of the divisions Basidiomycota (4.7%) and Cryptomycota (4.13%). Interestingly, we found that fungal communities did not exhibit significant changes with depth or chlorophyll concentration. However, they did vary with the latitudinal gradient in environmental conditions, which is characterized by high temperature (ranging from 22.3 to 27.0°C) and salinity (ranging from 38.0 to 40.4 PSU). Specifically, the proportions of Chytridomycetes and Neocallimastigomycetes (the two dominant classes of Chytridiomycota) were negatively correlated between themselves. Chytridomycetes exhibited a negative correlation with temperature (R2 = 0.60, p = 0.0028) and a positive correlation with salinity (R2 = 0.49, p = 0.010), being more abundant in the northern Red Sea. Conversely, Neocallimastigomycetes showed an increase in abundance with increasing temperature (R2 = 0.61, p = 0.0026) and a decrease with increasing salinity (R2 = 0.40, p = 0.026), making them more prevalent in the southern Red Sea. Overall, our study described a differential distribution of the most dominant fungal classes, with potential significance in their control of planktonic populations and consequent influence in the carbon cycle in the Red Sea ecosystem. These findings underscore the importance of further research to better understand the role of marine fungi in ecosystem functioning.We thank the technical personnel of the Bioscience Core Laboratory and the Coastal and Marine Resources Core Laboratory (CMR) and the RV Thuwal and RV Al-Azizi crews for their help during samplingThe author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This study was
funded by King Abdullah University of Science and Technology (KAUST) through baseline funding provided to SA
Olfactory Training for Post-COVID-19 Olfactory Dysfunction: A Meta-Analysis of Efficacy and Combination Therapies
No full textBackground/Objectives: This systematic review and meta-analysis evaluated the effectiveness of olfactory training (OT) using standardized protocols in patients with post-COVID-19 olfactory dysfunction. The objective was to assess whether OT, compared to no treatment, placebo, or alternative therapies, improved olfactory function as measured using validated smell tests, including UPSIT, Sniffin’ Sticks (TDI score), CCCRC, and B-SIT. Methods: A systematic search of PubMed, Web of Science, and Ovid Medline was conducted through February 2025 in accordance with PRISMA guidelines. Eight randomized controlled trials (RCTs) met the inclusion criteria. Data were extracted on study characteristics (author, year, country, design, sample size), population details (age, sex, post-COVID-19 cause), intervention type (training method, frequency, duration), comparators, outcome measures (baseline and post-intervention olfactory scores), follow-up duration, and reported adverse effects. The risk of bias was assessed using the Joanna Briggs Institute critical appraisal tool. Meta-analyses were performed using RevMan and Open Meta-Analyst. Results: Olfactory training significantly improved the olfactory scores compared to those of the controls. The greatest improvement was observed when OT was combined with PEA-luteolin (MD = 4.62, 95% CI: 2.17–7.06, p = 0.0002), followed by EDTA (MD = 2.33, 95% CI: 0.58–4.08, p = 0.009). Corticosteroids showed a borderline benefit (MD = 1.34, 95% CI: 0.01–2.67, p = 0.05), while alpha-lipoic acid had no significant effect. Combination therapies were associated with higher recovery rates (RR = 1.65, 95% CI: 1.13–2.42, p = 0.01). Conclusions: Olfactory training is an effective treatment for post-COVID-19 smell dysfunction. When paired with specific adjunct therapies, particularly PEA-luteolin, it may yield superior recovery outcomes. Further large-scale, standardized RCTs are needed to define optimal treatment protocols
Cardiolipin enhances the enzymatic activity of cytochrome bd and cytochrome bo <sub>3</sub> solubilized in dodecyl-maltoside
No full textCardiolipin (CL) is a lipid that is found in the membranes of bacteria and the inner membranes of mitochondria. CL can increase the activity of integral membrane proteins, in particular components of respiratory pathways. We here report that CL activated detergent-solubilized cytochrome bd, a terminal oxidase from Escherichia coli. CL enhanced the oxygen consumption activity ~ twofold and decreased the apparent KM value for ubiquinol-1 as substrate from 95 µM to 35 µM. Activation by CL was also observed for cytochrome bd from two Gram-positive species, Geobacillus thermodenitrificans and Corynebacterium glutamicum, and for cytochrome bo3 from E. coli. Taken together, CL can enhance the activity of detergent-solubilized cytochrome bd and cytochrome bo3.BT/Biocatalysi
Rapid and Highly Stable Membrane Reconstitution by LAiR Enables the Study of Physiological Integral Membrane Protein Functions
No full textFunctional reintegration into lipid environments represents a major challenge for in vitro investigation of integral membrane proteins (IMPs). Here, we report a new approach, termed LMNG Auto-insertion Reintegration (LAiR), for reintegration of IMPs into lipid bilayers within minutes. The resulting proteoliposomes displayed an unprecedented capability to maintain proton gradients and long-term stability. LAiR allowed for monitoring catalysis of a membrane-bound, physiologically relevant polyisoprenoid quinone substrate by Escherichia coli cytochromes bo3 (cbo3) and bd (cbd) under control of the proton motive force. LAiR also facilitated bulk-phase detection and physiological assessment of the “proton leak” in cbo3, a controversial catalytic state that previously was only approachable at the single-molecule level. LAiR maintained the multisubunit integrity and higher-order oligomeric states of the delicate mammalian F-ATP synthase. Given that LAiR can be applied to both liposomes and planar membrane bilayers and is compatible with IMPs and lipids from prokaryotic and eukaryotic sources, we anticipate LAiR to be applied broadly across basic research, pharmaceutical applications, and biotechnology.</p
