11 research outputs found
Synthesis of star-shape tetramine via thiol-ene click chemistry
No full textA large amount of energy to provide high temperature and high pressure are usually needed in the chemical reaction and separation process of traditional synthesis methods, which results in serious energy waste. However，click chemistry reactions, which have some unique advantages such as high efficiency, high reaction speed and mild condition, are expected to address these challenges. In this paper, a new type star-shape tetramine was synthesized by Thiol-ene click reaction of pentaerythritol tetraacryalte (PET4A) and cysteamine. The obtained star-shape tetramine could be used as an intermediate of dendrimers. In this reaction, PET4A was synthesized by the esterification of pentaerythritol and acrylic acid. IR and 1H NMR were used to characterize the structure of the obtained products
A Characterization of the RNA Modification Response to Starvation under Low Temperatures in Large Yellow Croaker (Larimichthys crocea)
No full textEmerging evidence shows that N6-methyladenosine (m6A) is a post-transcriptional RNA
modification that plays a vital role in regulation of gene expression, fundamental biological processes,
and physiological functions. To explore the effect of starvation on m6A methylation modification in
the liver of Larimichthys crocea (L. crocea) under low temperatures, the livers of L. crocea from cold and
cold + fasting groups were subjected to MeRIP-seq and RNA-seq using the NovaSeq 6000 platform.
Compared to the cryogenic group, the expression of RNA methyltransferases mettl3 and mettl14 was
upregulated, whereas that of demethylase fto and alkbh5 was downregulated in the starved cryogenic
group. A Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed that
the differentially m6A-modified genes were mainly enriched in steroid biosynthesis, DNA replication,
ribosome biogenesis in eukaryotes, PPAR, ECM-receptor interaction, lysine degradation,
phosphatidylinositol, and the MAPK signaling pathway, suggesting that L. crocea responds to starvation
under low-temperature stress through m6A methylation modification-mediated cell growth,
proliferation, innate immunity, and the maintenance of lipid homeostasis. This study advances
understanding of the physiological response mechanism exerted by m6A methylation modification
in starved L. crocea at low temperatures
Tracking interacting targets with laser scanner via on-line supervised learning
No full textSuccessful multi-target tracking requires locating the targets and labeling their identities. For the laser based tracking system, the latter becomes significantly more challenging when the targets frequently interact with each other. This paper presents a novel online supervised learning based method for tracking interacting targets with laser scanner. When the targets do not interact with each other, we collect samples and train a classifier for each target. When the targets are in close proximity, we use these classifiers to assist in tracking. Different evaluations demonstrate that this method has a better tracking performance than previous methods when interactions occur, and can maintain correct tracking under various complex tracking situations.Automation & Control SystemsRoboticsEICPCI-S(ISTP)1
A Characterization of the RNA Modification Response to Starvation under Low Temperatures in Large Yellow Croaker (Larimichthys crocea)
No full textEmerging evidence shows that N6-methyladenosine (m6A) is a post-transcriptional RNA modification that plays a vital role in regulation of gene expression, fundamental biological processes, and physiological functions. To explore the effect of starvation on m6A methylation modification in the liver of Larimichthys crocea (L. crocea) under low temperatures, the livers of L. crocea from cold and cold + fasting groups were subjected to MeRIP-seq and RNA-seq using the NovaSeq 6000 platform. Compared to the cryogenic group, the expression of RNA methyltransferases mettl3 and mettl14 was upregulated, whereas that of demethylase fto and alkbh5 was downregulated in the starved cryogenic group. A Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed that the differentially m6A-modified genes were mainly enriched in steroid biosynthesis, DNA replication, ribosome biogenesis in eukaryotes, PPAR, ECM-receptor interaction, lysine degradation, phosphatidylinositol, and the MAPK signaling pathway, suggesting that L. crocea responds to starvation under low-temperature stress through m6A methylation modification-mediated cell growth, proliferation, innate immunity, and the maintenance of lipid homeostasis. This study advances understanding of the physiological response mechanism exerted by m6A methylation modification in starved L. crocea at low temperatures
Delirium in the Emergency Department and Its Extension into Hospitalization (DELINEATE) Study: Effect on 6-month Function and Cognition
No full textBacteria-microalgae interactions from an evolutionary perspective and their biotechnological significance
No full textInteractions between bacteria and microalgae have been studied in natural environments and in industrial consortia. As results of co-evolution for millions of years in nature, they have developed complex symbiotic relationships, including mutualism, commensalism and parasitism, the nature of which is decided by mechanisms of the interaction. There are two main types of molecular interactions between microalgae and bacteria: exchange of nutrients and release of signalling molecules. Nutrient exchange includes transport of organic carbon from microalgae to bacteria and nutrient nitrogen released from nitrogen-fixing bacteria to microalgae, as well as reciprocal supply of micronutrients such as B vitamins and iron. Signalling molecules such as phytohormones secreted by microalgae and quorum sensing molecules secreted by bacteria have been shown to positively affect growth and metabolism of the symbiotic partner. However, there are still a number of potential microalgaebacteria interactions that have not been well explored, including cyclic peptides, other quorum signalling molecules, and extracellular vesicles involved in exchange of genetic materials. A more thorough understanding of these interactions may not only result in a deeper understanding of the relationships between these symbiotic organisms but also have potential biotechnological applications. Upon new mechanisms of interaction being identified and characterized, novel bioprocesses of synthetic ecology might be developed especially for wastewater treatment and production of biofertilizers and biofuels
Systemic antibiotics increase microbiota pathogenicity and oral bone loss
No full textAbstract Periodontitis is the most widespread oral disease and is closely related to the oral microbiota. The oral microbiota is adversely affected by some pharmacologic treatments. Systemic antibiotics are widely used for infectious diseases but can lead to gut dysbiosis, causing negative effects on the human body. Whether systemic antibiotic-induced gut dysbiosis can affect the oral microbiota or even periodontitis has not yet been addressed. In this research, mice were exposed to drinking water containing a cocktail of four antibiotics to explore how systemic antibiotics affect microbiota pathogenicity and oral bone loss. The results demonstrated, for the first time, that gut dysbiosis caused by long-term use of antibiotics can disturb the oral microbiota and aggravate periodontitis. Moreover, the expression of cytokines related to Th17 was increased while transcription factors and cytokines related to Treg were decreased in the periodontal tissue. Fecal microbiota transplantation with normal mice feces restored the gut microbiota and barrier, decreased the pathogenicity of the oral microbiota, reversed the Th17/Treg imbalance in periodontal tissue, and alleviated alveolar bone loss. This study highlights the potential adverse effects of long-term systemic antibiotics-induced gut dysbiosis on the oral microbiota and periodontitis. A Th17/Treg imbalance might be related to this relationship. Importantly, these results reveal that the periodontal condition of patients should be assessed regularly when using systemic antibiotics in clinical practice
Intermedin suppresses pressure overload cardiac hypertrophy through activation of autophagy.
No full textLeft ventricular hypertrophy is a maladaptive response to pressure overload and an important risk factor for heart failure. Intermedin (IMD), a multi-functional peptide, plays important roles in cardiovascular protection. In this study, we revealed an autophagy-dependent mechanism involved in IMD's protection against cardiac remodeling and cardiomyocyte death in heart hypertrophy. We observed that transverse aortic contraction (TAC) induction, Ang II or ISO exposure induced remarkable increase in the expression of endogenous IMD and its receptor components, CRLR, RAMP1 and RAMP3, in mouse hearts and H9c2 cell cultures, respectively. Furthermore, the heart size, heart weight/body weight ratios, cardiomyocyte size and apoptosis, interstitial collagen, hypertrophic markers including ANP and BNP expression were also significantly increased, which were effectively suppressed by IMD supplementation. In addition, IMD induced capillary angiogenesis and improved functions in hypertrophic hearts. We further observed that IMD induced strong autophagy in hypertrophic hearts and cultured cells, which was paralleling with the decrease in cardiomyocyte size and apoptosis. Furthermore, an autophagy inhibitor, 3-MA, was used to block the IMD-augmented autophagy level, and then the protection of IMD on cardiomyocyte hypertrophy and apoptosis was almost abrogated. We also observed that IMD supplementation stirred intracellular cAMP production, and augmented the ERK1/2 phosphorylation induced by Ang II/ISO exposure in H9c2 cells. In addition, we inhibited PI3K, PKA and MAPK/ERK1/2 signaling pathways by using wortamannin, H89 and PD98059, respectively, in H9c2 cells co-incubating with both IMD and Ang II or ISO, and observed that these inhibitors effectively reduced IMD-augmented autophagy level, but only H89 and PD98059 pre-incubation abrogated the anti-apoptotic action of IMD. These results indicate that the endogenous IMD and its receptor complexes are induced in hypertrophic cardiomyocytes and proposed to play an important role in the pathogenesis of cardiac hypertrophy, and the autophagy stirred by IMD supplementation is involved in its protection against cardiomyocyte hypertrophy and apoptosis through the activation of both cAMP/PKA and MAPK/ERK1/2 pathways
The small HDL particle hypothesis of Alzheimer's disease
No full textWe propose the hypothesis that small high-density lipoprotein (HDL) particles reduce the risk of Alzheimers disease (AD) by virtue of their capacity to exchange lipids, affecting neuronal membrane composition and vascular and synaptic functions. Concentrations of small HDLs in cerebrospinal fluid (CSF) and plasma were measured in 180 individuals ≥60 years of age using ion mobility methodology. Small HDL concentrations in CSF were positively associated with performance in three domains of cognitive function independent of apolipoprotein E (APOE) ε4 status, age, sex, and years of education. Moreover, there was a significant correlation between levels of small HDLs in CSF and plasma. Further studies will be aimed at determining whether specific components of small HDL exchange across the blood, brain, and CSF barriers, and developing approaches to exploit small HDLs for therapeutic purposes
