945 research outputs found
sj-docx-1-aph-10.1177_10105395221139346 – Supplemental material for Association Between Changes in Family Life Due to COVID-19 and Depressive Mood and Stress Perception
No full textSupplemental material, sj-docx-1-aph-10.1177_10105395221139346 for Association Between Changes in Family Life Due to COVID-19 and Depressive Mood and Stress Perception by Min-Su Kim, Mi Ah Han, Jong Park and So Yeon Ryu in Asia Pacific Journal of Public Health</p
Regulation of Psc-Su(z)2 genes in drosophila melanogaster
No full textThe Psc and Su(z)2 genes belong to the Polycomb Group (PcG) and Psc itself is one of the core components of the PRC1 repressive complex and genetic evidence suggests that it is autoregulated by PcG mechanisms. Recently, the Psc-Su(z)2 region was also found to contain several putative Polycomb Response Elements that bind PcG proteins while the entire region is enriched for H3K27me3. Current model of PcG mechanism is all-or- none silencing paradigm derived from its role in homeotic gene regulation. However, it is likely that PcG regulation at many other target genes functions by down-regulating rather than silencing expression. This is certainly the case for PcG genes like Psc and Su(z)2. To understand how PcG mechanisms down-regulate a target gene, the nature of the PcG binding sites in the Psc-Su(z)2 region and their repressive effects were analyzed, using reporter gene construct. There are at least two functional PREs that can silence a reporter gene in a PcG-dependent manner and one of them can also show anti-silencing activity, depending on the chromosomal context. In addition, we found a down-regulation module in the vicinity of Psc promoter, whose effect is insensitive to the dosage of PcG genes. Probably, several different regulatory elements might be cooperative to down-regulate Psc-Su(z)2 genes by PcG mechanism. I have also generated small deletions that remove one such binding peak. Deletion of one of the Psc-Su(z)2 PREs increases the expression level of Psc and Su(z)2 by 2-3 fold at late embryonic stage. Perhaps the increased expression of PSC can partially compensate by binding to the other PREs in the Psc-Su(z)2 locus. On the contrary, the expressions of CG13323 and CG13324 genes behind of the PRE are decreased by 20 to 50 fold during entire embryonic stages. Also, the chromatin IP experiment showed that the loss of this fragment extends the domain of H3K27me3 around 10kb further, to a region that includes both of CG13323 and CG13324 transcripts. So, the fragment removed in both deletions may not possess only PRE but also a transcriptional enhancer for downstream genes or boundary element to block the PcG silencing.Ph.D.Includes bibliographical references (p. 92-99)by Sung-Yeon Par
Estimating Hourly Population Distribution Patterns at High Spatiotemporal Resolution in Urban Areas Using Geo-Tagged Tweets and Dasymetric Mapping
No full textThis paper introduces a spatiotemporal analysis framework for estimating hourly changing population distribution patterns in urban areas using geo-tagged tweets (the messages containing users’ geospatial locations), land use data, and dasymetric maps. We collected geo-tagged social media (tweets) within the County of San Diego during one year (2015) by using Twitter’s Streaming Application Programming Interfaces (APIs). A semi-manual Twitter content verification procedure for data cleaning was applied first to separate tweets created by humans from non-human users (bots). The next step was to calculate the number of unique Twitter users every hour within census blocks. The final step was to estimate the actual population by transforming the numbers of unique Twitter users in each census block into estimated population densities with spatial and temporal factors using dasymetric maps. The temporal factor was estimated based on hourly changes of Twitter messages within San Diego County, CA. The spatial factor was estimated by using the dasymetric method with land use maps and 2010 census data. Comparing to census data, our methods can provide better estimated population in airports, shopping malls, sports stadiums, zoo and parks, and business areas during the day time
High-yield synthesis and purification of recombinant human GABA transaminase for high-throughput screening assays
No full text© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.Many studies have focussed on modulating the activity of γ-aminobutyric acid transaminase (GABA-T), a GABA-catabolizing enzyme, for treating neurological diseases, such as epilepsy and drug addiction. Nevertheless, human GABA-T synthesis and purification have not been established. Thus, biochemical and drug design studies on GABA-T have been performed by using porcine GABA-T mostly and even bacterial GABA-T. Here we report an optimised protocol for overexpression of 6xHis-tagged human GABA-T in human cells followed by a two-step protein purification. Then, we established an optimised human GABA-T (0.5 U/mg) activity assay. Finally, we compared the difference between human and bacterial GABA-T in sensitivity to two irreversible GABA-T inhibitors, gabaculine and vigabatrin. Human GABA-T in homodimeric form showed 70-fold higher sensitivity to vigabatrin than bacterial GABA-T in multimeric form, indicating the importance of using human GABA-T. In summary, our newly developed protocol can be an important first step in developing more effective human GABA-T modulators.11Nsciescopu
Between independent cinema and blockbuster in South Korea: Yeon Sang-Ho's films and their reception in Spain
No full textEl cineasta Yeon Sang-ho supone un caso paradigmático en el cine surcoreano contemporáneo. Su trayectoria profesional, ligada desde el inicio a la industria independiente a través de la animación, cambió radicalmente con el salto al cine comercial gracias a su primer live-action, Tren a Busan (Busanhaeng, 2016). Desde ese momento, Yeon se convirtió en uno de los directores más afamados de Corea del Sur, conquistando, a su vez, a la crítica y al público internacional. Al respecto, este artículo investiga cómo las obras de Yeon Sang-ho han colaborado en la creación y afianzamiento de una marca de calidad implícita en el cine surcoreano, que, a su vez, es asimilada tanto por la crítica como por el cinéfilo español. Por ello, se pretende observar la evolución de la recepción del cine surcoreano a través de la prensa generalista y especializada a largo de estas tres últimas décadas. Asimismo, se analiza la visión global que ésta ha emitido sobre su filmografía para extraer las tendencias generales entre la crítica y el público español como parte de la recepción del cine surcoreano.Filmmaker Yeon Sang-ho is a paradigmatic case in contemporary South Korean cinema. His professional career, linked from the beginning to the independent animation industry, changed radically with the leap to commercial cinema thanks to his first live-action, Train to Busan (Busanhaeng, 2016). From that moment on, Yeon became one of South Korea’s most famous directors, winning over critics and international audiences. In this respect, this article investigates how the films of Yeon Sang-ho have collaborated in the creation and strengthening of a quality brand implicit in South Korean cinema, which, in turn, is assimilated by both critics and Spanish cinephiles. For this reason, we can observe the evolution of the reception of South Korean cinema through the generalist and specialised press over the last three decades. It also analyses the global vision that it has emitted on its filmography to extract the general trends between critics and the Spanish public as part of the reception of South Korean cinema in Spain
Post-transcriptional regulation of SHANK3 expression by microRNAs related to multiple neuropsychiatric disorders
No full textBackground: Proper neuronal function requires tight control of gene dosage, and failure of this process underlies the pathogenesis of multiple neuropsychiatric disorders. The SHANK3 gene encoding core scaffolding proteins at glutamatergic postsynapse is a typical dosage-sensitive gene, both deletions and duplications of which are associated with Phelan-McDermid syndrome, autism spectrum disorders, bipolar disorder, intellectual disability, or schizophrenia. However, the regulatory mechanism of SHANK3 expression in neurons itself is poorly understood. Results: Here we show post-transcriptional regulation of SHANK3 expression by three microRNAs (miRNAs), miR-7, miR-34a, and miR-504. Notably, the expression profiles of these miRNAs were previously shown to be altered in some neuropsychiatric disorders which are also associated with SHANK3 dosage changes. These miRNAs regulated the expression of SHANK3 and other genes encoding actin-related proteins that interact with Shank3, through direct binding sites in the 3' untranslated region (UTR). Moreover, overexpression or inhibition of miR-7 and miR-504 affected the dendritic spines of the cultured hippocampal neurons in a Shank3-dependent manner. We further characterized miR-504 as it showed the most significant effect on both SHANK3 expression and dendritic spines among the three miRNAs. Lentivirus-mediated overexpression of miR-504, which mimics its reported expression change in postmortem brain tissues of bipolar disorder, decreased endogenous Shank3 protein in cultured hippocampal neurons. We also revealed that miR-504 is expressed in the cortical and hippocampal regions of human and mouse brains. Conclusions: Our study provides new insight into the miRNA-mediated regulation of SHANK3 expression, and its potential implication in multiple neuropsychiatric disorders associated with altered SHANK3 and miRNA expression profiles117161sciescopu
Dynamic Ca(2+)-Dependent Stimulation of Vesicle Fusion by Membrane-Anchored Synaptotagmin 1
No full textIn neurons, synaptotagmin 1 (Syt1) is thought to mediate the fusion of synaptic vesicles with the plasma membrane when presynaptic Ca2+ levels rise. However, in vitro reconstitution experiments have failed to recapitulate key characteristics of Ca2+-triggered membrane fusion. Using an in vitro single-vesicle fusion assay, we found that membrane-anchored Syt1 enhanced Ca2+ sensitivity and fusion speed. This stimulatory activity of membrane-anchored Syt1 dropped as the Ca2+ level rose beyond physiological levels. Thus, Syt1 requires the membrane anchor to stimulate vesicle fusion at physiological Ca2+ levels and may function as a dynamic presynaptic Ca2+ sensor to control the probability of neurotransmitter release
DynamicLabels: Supporting Informed Construction of Machine Learning Label Sets with Crowd Feedback
No full textLabel set construction - deciding on a group of distinct labels - is an essential stage in building a supervised machine learning (ML) application, as a badly designed label set negatively affects subsequent stages, such as training dataset construction, model training, and model deployment. Despite its significance, it is challenging for ML practitioners to come up with a well-defined label set, especially when no external references are available. Through our formative study (n=8), we observed that even with the help of external references or domain experts, ML practitioners still need to go through multiple iterations to gradually improve the label set. In this process, there exist challenges in collecting helpful feedback and utilizing it to make optimal refinement decisions. To support informed refinement, we present DynamicLabels, a system that aims to support a more informed label set-building process with crowd feedback. Crowd workers provide annotations and label suggestions to the ML practitioner's label set, and the ML practitioner can review the feedback through multi-aspect analysis and refine the label set with crowd-made labels. Through a within-subjects study (n=16) using two datasets, we found that DynamicLabels enables better understanding and exploration of the collected feedback and supports a more structured and flexible refinement process. The crowd feedback helped ML practitioners explore diverse perspectives, spot current weaknesses, and shop from crowd-generated labels. Metrics and label suggestions in DynamicLabels helped in obtaining a high-level overview of the feedback, gaining assurance, and spotting surfacing conflicts and edge cases that could have been overlooked. © 2024 Owner/Author
소셜네트워크에서의 인플루언서 영향력 검증에 관한 연구 : 페이스북 어플리케이션 확산을 중심으로
No full text학위논문(석사) - 한국과학기술원 : 문화기술대학원, 2009.2, [ iv, 47 p. ]This research proposes and evaluates a methodology for discovering influencers within online social networking sites (OSNSs), which provide powerful means of sharing common interests and communicating between users. Finding outstanding influencers out is major perspective in success of viral marketing. By using data from Facebook, one of the most popular online social networking sites, we validate the effects of influencers extracted by the methodology.
Proposed methodology for discovering influencers consists of two-step process. In step one, structural analysis based on the social graph which connects users who are friends each other is performed. Through the degree centrality computation, we choose the influencer candidate subgroup. Next, the second step is activity analysis using user’s activity history. After whole process, we finally obtain a few powerful influencers who can affect more effectively other people than mediocrities.
The positive effects of influencers determined from the methodology are confirmed by experiments that disseminate flash games on Facebook.
Our research lays the groundwork for searching influencers within online social networking sites. And proposed methodology contributes to competitive strategies in viral marketing.한국과학기술원 : 문화기술대학원
Transmutations of the human being through South Korean animated films: Seoul Station (Yeon Sang-ho, 2016) and Beauty Water (Cho Kyung-hun, 2020)
No full text[EN] With considerable international recognition, South Korean animated cinema is increasingly trying to distance itself from its traditional Japanese influences in order to mark a distinction through films aimed at adult audiences. Although its arrival in Spain was limited until 2010, festivals and digital platforms have become the perfect allies for its access. It is this cinema that is responsible for offering a critical view of its context, just as the New South Korean Cinema did in its time after its emergence at the end of the 1990s. This article analyses the animated films Seoul Station (Yeon Sang-ho, 2016) and Beauty Water (Cho Kyung-hun, 2020), which arrived in Spain through the Sitges Festival and the Filmin platform, respectively, and which offer a pessimistic vision of today's society through the representation of the transmutation of the body.[ES] Con un considerable reconocimiento internacional, el cine de animación surcoreano se distancia cada vez más de sus tradicionales influencias japonesas para marcar una distinción a través de las películas destinadas al público adulto. Pese a que su llegada a España fue limitada hasta 2010, los festivales y las plataformas digitales se han convertido en los perfectos aliados para su acceso. Es, este cine, el encargado de ofrecer una mirada crítica de su contexto, al igual que hiciera en su momento el Nuevo Cine Surcoreano tras surgir a finales de los 90. Este artículo analiza dicha mirada crítica por medio de las películas de animación Seoul Station (Yeon Sang-ho, 2016) y Beauty Water (Cho Kyung-hun, 2020), que llegaron a España a través del Festival de Sitges y la plataforma Filmin, respectivamente, y que ofrecen una visión pesimista de la sociedad actual a través de la representación de la transmutación del cuerpo.Dueñas Mohedas, S. (2022). Transmutaciones del ser humano a través del cine de animación de Corea del Sur: Seoul Station (Yeon Sang-ho, 2016) y Beauty Water (Cho Kyung-hun). Con A de animación. (15):100-115. https://doi.org/10.4995/caa.2022.17895OJS10011515BOON, Kevin Alexander, 2007. “Ontological Anxiety Made Flesh: The Zombie in Literature, Film and Culture”, en SCOTT (ed.), Monsters and the Monstrous. Myths and Metaphors of Enduring Evil, Amsterdam y Nueva York: Rodopi, pp. 33-43. https://doi.org/10.1163/9789401204811_004CHIE, Yamanaka, 2013, “Manhwa in Korea: (Re-)Nationalizing Comics Culture”, en BERNDT, KÜMMERLING-MEIBAUER (eds.), Manga’s Cultural Crossroads, Nueva York: Routledge, pp. 85-99.CHO, Kyung-hun, 2020. Beauty Water, Corea del Sur: SS Animent y Studio ANIMAL Co. Ltd.CHOO, Kukhee, 2014, “Hyperbolic Nationalism: South Korea's Shadow Animation Industry”, en Mechademia, nº 9, pp. 144-162. https://doi.org/10.5749/mech.9.2014.0144CUÉLLAR BARONA, Margarita, 2008. “La figura del monstruo en el cine de horror”, en Revista CS, nº 2, pp. 227-246. Recuperado de: http://www.scielo.org.co/pdf/recs/n2/n2a10.pdf . https://doi.org/10.18046/recs.i2.419DUEÑAS MOHEDAS, Sonia, 2020. “Entre el cine independiente y el blockbuster en Corea del Sur: Las obras de Yeon Sang-ho y su recepción en España”, en L’Atalante: Revista de estudios cinematográficos, nº 29, pp. 67-84.ENGUIX GRAU, Begonya, GONZÁLEZ RAMOS, Ana María, 2018. “Cuerpos, mujeres y narrativas: Imaginando corporalidades y géneros”, en Athenea Digital, nº 18(2), pp. 1-31. https://doi.org/10.5565/rev/athenea.1956GÓMEZ GURPEGUI, Carlos, 2015. “El realismo animado de Sang-ho Yeon”, en Con A de animación, nº 5, pp. 126-139. ttps://doi.org/10.4995/caa.2015.3548.IMBERT, Gerárd, 2019. Crisis de valores en el cine posmoderno (Más allá de los límites). Madrid: Cátedra.JANG, Sung-ran, 2016. “‘Busan’ director reflects on film’s wild ride: Yeon Sang-ho’s first live-action movie was a historic success - so why is he still worrying?”, en Korea JoongAng Daily, 10 de agosto de 2006 (https://koreajoongangdaily.joins.com/2016/08/10/movies/Busan-director-reflects-on-films-wild-ride-Yeon-Sanghos-first-liveaction-movie-was-a-historic-success-so-why-is-he-still-worrying/3022477.html [Acceso: 28 de mayo de 2021])KIM, Tae Young, JIN, Dal Yong, 2016. “Cultural policy in the Korean wave: an analysis of cultural diplomacy embedded in presidential speeches”, en International Journal of Communication, nº 10, pp. 14–34. Recuperado de: https://ijoc.org/index.php/ijoc/article/view/5128LEE, Sung-Ae, 2019. “The New Zombie Apocalypse and Social Crisis in South Korean Cinema”, en Observatori: Centre d’Estudis Australians i Transnacionals, nº 27, pp: 150-166.MARTIN, Daniel, 2018. “South Korean animation today: national identity and the appeal to local audiences”, en Journal of Japanese and Korean Cinema, vol. 10, nº 2, pp. 92-97. https://doi.org/10.1080/17564905.2018.1518691NANCY, Jean-Luc. 2003 [1992]. Corpus, Patricio Bulnes, Madrid: Arena Libros (Corpus, París: Éditions Métailié).OCAÑA, Javier. 2014. “Sectas con lápices de colores”, en El País, 16 de mayo de 2014, (https://elpais.com/cultura/2014/05/15/actualidad/1400169840_660670.html [Acceso: 1 de junio de 2021]).SALVÀ, Nando, 2017. “‘Seoul station’: lo infecto es la sociedad”, en El Periódico, 4 de mayo de 2017, (https://www.elperiodico.com/es/ocio-y-cultura/20170504/criti-ca-pelicula-seoul-station-yeon-sang-hoo-6013831 [Acceso: 3 de junio de 2021]).SANTAEULARIA, Isabel, 2009. El monstruo humano. Una introducción a la ficción de los asesinos en serie. Barcelona: Laertes.ROCHE CÁRCEL, Juan Antonio, 2017. “Crisis y miedo al otro en el cine de terror. El caso de ‘King Kong’”, en Signa: Revista de la Asociación Española de la Semiótica, nº 26, 2017, pp. 511-538. https://doi.org/10.5944/signa.vol26.2017.19936SCOTT-BARON, Hayden, 2006. Manga Clip Art: Everything You Need to Create Your Own Professional-Looking Manga Artwork. Kansas City: Andrews McMeel Publishing.TABOADA, Pablo G., 2016. “[Gijón 2016] Día 3: Doncellas coreanas y zombis que viajan en tren”, en Cinemanía, 22 de noviembre de 2016, (https://www.20minutos.es/cinemania/noticias/gijon-2016-dia-3-doncellas-coreanas-y-zombis-que-viajan-en-tren-71151/ [Acceso: 4 de junio de 2021]).YEON, Sang-ho, 2011. The King of Pigs, Corea del Sur: Studio Dadashow.YOON, Hyejin, 2019. “Do higher skills result in better jobs? The case of the Korean animation industry”, en Geoforum, vol. 99, pp. 267-277. https://doi.org/10.1016/j.geoforum.2018.08.01
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