882 research outputs found
sj-pdf-1-imr-10.1177_03000605221089503 - Supplemental material for MicroRNAs as potential biomarkers for the diagnosis of inflammatory bowel disease: a systematic review and meta-analysis
No full textSupplemental material, sj-pdf-1-imr-10.1177_03000605221089503 for MicroRNAs as potential biomarkers for the diagnosis of inflammatory bowel disease: a systematic review and meta-analysis by Lina Sun, Yanan Han, Hua Wang, Huanyu Liu, Shan Liu, Hongbin Yang, Xiaoxia Ren and Ying Fang in Journal of International Medical Research</p
Supplemental Material - Effect of Physiotherapy Interventions on Motor Symptoms in People With Parkinson’s Disease: A Systematic Review and Meta-Analysis
No full textSupplemental Material for Effect of Physiotherapy Interventions on Motor Symptoms in People with Parkinson’s Disease: A Systematic Review and Meta-Analysis by Yajie Yang, Yang Wang, Tianzi Gao, Abudurousuli Reyila, Jiaxin Liu, Jiajia Liu, and Hongbin Han in Biological Research For Nursing</p
sj-docx-1-tan-10.1177_17562864221104508 – Supplemental material for Prediction of the generalization of myasthenia gravis with purely ocular symptoms at onset: a multivariable model development and validation
No full textSupplemental material, sj-docx-1-tan-10.1177_17562864221104508 for Prediction of the generalization of myasthenia gravis with purely ocular symptoms at onset: a multivariable model development and validation by Feng Li, Hongbin Zhang, Ya Tao, Frauke Stascheit, Jiaojiao Han, Feng Gao, Hongbo Liu, Alberto Carmona-Bayonas, Zhongmin Li, Jens-C. Rueckert, Andreas Meisel and Song Zhao in Therapeutic Advances in Neurological Disorders</p
The Effect of the One-Child Policy on Fertility in China: Identification Based on the Differences-in-Differences
No full textThis paper measures the effect of China's one-child policy on fertility by exploring the natural experiment that has been created by China's unique affirmative birth control policy, which is possibly the largest social experiment in human history. Because the one-child policy only applied to Han Chinese, but not to ethnic minorities, we construct a differences-in-differences estimator to identify the effect of the policy on fertility. Such a natural experiment is a rare opportunity, whether for the analysis of the effect on fertility or for the analysis of economics in general. Using two rounds of the Chinese Population Census, we find that the one-child policy has had a large effect on fertility. The average effect on the post-treatment cohorts on the probability of having a second child is as large as -11 percentage points. We also find that the magnitude is larger in urban areas and for more educated women. Our robustness tests suggest that our differences-in-differences estimates of the effect of the one-child policy are not very likely to be driven by other policy or socio-economic changes that have affected the Han and the minorities differently.
Investigating force-induced unfolding and folding of proteins at the single molecule level
No full textUnderstanding the molecular mechanism of protein folding has always remained a challenging problem. Extensive investigations based on ensemble methods have been carried out to shed light on the protein folding problem. Compared with ensemble-averaged measurements, single-molecule force spectroscopy (SMFS) has evolved as a powerful technique to probe mechanical unfolding/folding of proteins at the single-molecule level, which provides rich details of protein conformational changes. Optical tweezers (OT), as one representative SMFS technique, have been widely used to directly monitor a protein folding process. This thesis presents a series of studies that use OT to investigate the mechanical unfolding/folding of two distinct classes of proteins: knotted/slipknotted proteins and Ca²⁺-binding metalloproteins.
First, we combined OT and steered molecular dynamics (SMD) simulations to investigate a complex slipknotted protein. When stretched from the N- and C-termini, the slipknotted structure could be completely untied via multiple unfolding pathways. Upon relaxation, this protein showed complex folding behaviors involving misfolding. Our studies demonstrate the unfolding/folding mechanisms of a slipknotted protein with a complex topological conformation.
Second, we used OT to mechanically tighten, untie and retie a trefoil knotted protein. Our results revealed that protein folding with a preformed knot was fast and robust; however, the folding from the unfolded polypeptide chain without the knot conformation was significantly slower, suggesting that the knotting was the rate-limiting step for the folding of this trefoil knotted protein.
Next, we investigated the mechanical unfolding/folding behaviors of a Ca²⁺-binding protein, RTX (repeats-in-toxin) block V. Our results elucidated the secretion mechanism of this RTX block V and revealed that the mechanical design can ensure an efficient translocation process.
Finally, we used OT to study another Ca²⁺-binding protein, RTX block IV. The mechanical properties of RTX block IV was well-characterized. Our results revealed that RTX block IV follows a similar translocation mechanism to be secreted as RTX block V. We also suggested that the secreted RTX block V is stabilized by folded RTX block IV and protected from being unfolded in vivo.
Overall, this thesis clarified the unfolding/folding mechanisms of two knotted/slipknotted proteins and Ca²⁺-binding protein RTX.Science, Faculty ofChemistry, Department ofGraduat
Codoping Zinc Oxide Nanowires
No full textabstract: The zinc oxide nanowires being grown are not developing properly and need to be fixed. In order to do this, the furnace equipment and experimental procedure must be tested until the results produced yield acceptable quality zinc oxide nanowires. After experimentation the nanowires were produced to an acceptable quality. With quality nanowires to experiment with, testing began to examine the effects of different thicknesses of aluminum dopants. Once doped and annealed, the wires were transferred to a substrate with a grid so contact points could be applied. However; the experiment was phased out once this step was half way complete due to the lab shifting to examine co-doping zinc oxide nanowires as explored in part two of this paper. The goal of co-doping zinc oxide film is to create an ideal p/n type relationship for power generation, so this project focuses on altering the electrical properties of zinc oxide through doping that will allow more energy to be generated from the solar panels than current zinc oxide solar panels. The zinc oxide film doped with manganese was sputtered onto a silicon substrate. The experiment failed to create a co-doped sample because an x-ray photoelectron spectroscopy reading of the sample proved no nitrogen existed in the zinc oxide doped with manganese film. This experiment leads into this research teams work with co-doping, so instead of viewing this project as a failure it is seen as a learning experience. The research team is examining the results and creating new experiments to run to fix the problem. I currently work with my mentor Dr. Hongbin Yu and Seung Ho Ahn while doing research
- …
