1,721,129 research outputs found
A method to visualize the microarchitecture of glycoprotein matrices with scanning electron microscopy
No full textThe paper describes a method to visualize special extracellular glycoprotein matrices, such as zona pellucida and mucus, by means of high resolution scanning electron microscopy with the aid of saponin and the cationic dye ruthenium red. Images of the zona pellucida of human mature oocytes as well as the mucus covering mouse jejunum obtained with conventional techniques are compared with images obtained after saponin, ruthenium red, and thiocarbohydrazide (T/O/T/O) treatment.This technique offers several advantages in terms of stabilization of glycoproteins and polyanionic carbohydrates, thus preserving the structure and morphology of the thin filaments forming these very special matrices. This technique can be usefully applied to investigate the morphodynamic changes of similar structures in various physiological and pathological conditions
Investigating Outbreaks of Salmonella Typhimurium Using Case-Control Studies, with a Reference to the One Health Approach
No full textAn outbreak is defined as the occurrence of disease cases in excess of normal expectancy within a particular area and a given time. Foodborne outbreaks caused by gastrointestinal bacteria such as Salmonella Typhimurium are among the most commonly reported and most extensively investigated. The classic outbreak investigation follows a series of well-defined steps which lead to a faster confirmation of the source and hopefully preventing of further cases. These steps are ideally undertaken using a One Health cross-sectorial collaboration approach involving partners from public health, food safety, and the veterinary and environmental sectors. In order to firmly identify the source of the outbreak, descriptive epidemiology is often combined with more robust evidence from analytical epidemiology such as a case-control study. A case-control study assesses whether a specific exposure is associated with illness, firstly by identifying cases (persons known to have been ill) and controls (persons who have not been ill, used as a reference group), and then retrospectively through interviews determining specific exposures for all persons. This information ultimately leads to the calculation of an odds ratio (see Note 3) which indicates the strength of the association between specific exposures and the outcome (illness or no illness). A well-conducted case-control study may substantiate or form core evidence as to the vehicle of a foodborne outbreak and is often a very important investigation tool, particularly in situations where microbiological proof cannot be obtained.</p
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
The Impact of Centrosome Abnormalities on Breast Cancer Development and Progression with a Focus on Targeting Centrosomes for Breast Cancer Therapy
No full textDithiothreitol prevents membrane fusion but not centrosome or microtubule organization during the first cell cycles in sea urchins
No full text
- …
