21 research outputs found

    New Insights into Placozoan Sexual Reproduction and Development

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    Unraveling animal life cycles and embryonic development is basic to understanding animal biology and often sheds light on phylogenetic relationships. A key group for understanding the evolution of the Metazoa is the early branching phylum Placozoa, which has attracted rapidly increasing attention. Despite over a hundred years of placozoan research the life cycle of this enigmatic phylum remains unknown. Placozoa are a unique model system for which the nuclear genome was published before the basic biology (i.e. life cycle and development) has been unraveled. Four organismal studies have reported the development of oocytes and one genetic study has nourished the hypothesis of sexual reproduction in natural populations at least in the past. Here we report new observations on sexual reproduction and embryonic development in the Placozoa and support the hypothesis of current sexual reproduction. The regular observation of oocytes and expressed sperm markers provide support that placozoans reproduce sexually in the field. Using whole genome and EST sequences and additional cDNA cloning we identified five conserved sperm markers, characteristic for different stages in spermatogenesis. We also report details on the embryonic development up to a 128-cell stage and new ultrastructural features occurring during early development. These results suggest that sperm and oocyte generation and maturation occur in different placozoans and that clonal lineages reproduce bisexually in addition to the standard mode of vegetative reproduction. The sum of observations is best congruent with the hypothesis of a simple life cycle with an alternation of reproductive modes between bisexual and vegetative reproduction

    Analisis Persepsi Masyarakat tentang BMT (Baitul Maal Wat Tamwil) di Kota Medan

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    This research deals with the public perception of the existence of Baitul Maal Wattamwil (BMT). This study aims to determine the benefits of the existence of Baitul Maal Wattamwil (BMT), to know the development of the Baitul Mal Wattamwil (BMT) in Medan, and to determine the public interest to be customer of Baitul Mal Wattamwil (BMT). The data which is used in this study is primary data that obtained from direct interviews and collected data by using a questionnaire. In this study, the method of analysis used is method of descriptive analysis, the author uses the computer program SPSS version 17.0 for data processing. Data were collected and the results are grouped in the form of tables and forms a diagram. The results showed that the reason for the ease in transaction and obtain customer information as a major factor in choosing the Baitul Mal Wattamwil (BMT) and the second factor is that its products are not opposed to religion. Addition of mudharabah financing products which is chosen by some customers because most customers come from entrepreneurs and housewives. The problem faced by the customer is the small of value loans as well as the only business that has been lent by the Baitul Mal Wattamwil (BMT). Besides Regardless of the problems faced by the customers related products, customers also benefit from the presence of Baitul Maal Wat Tamwil (BMT) in Medan, in example they can be helped business, additional capital, and can buy the goods they need with the terms that does not burden the customer itself.87 HalamanSkripsi Sarjan

    GLAUCOME CHRONIQUE AU MAROC : GRAND DILEMME ENTRE UNE PRATIQUE MEDICALE OPTIMISEE ET LES CONTRAINTES DU PATIENT

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    Les différentes formes de glaucome chronique à angle ouvert représentent un problème majeur de santé publique. En l’absence d’études fiables récentes sur la prévalence de la maladie glaucomateuse et ses facteurs de risque, il s’avère difficile de définir une stratégie efficiente pour la lutte contre cette maladie dans notre pays, où le glaucome est la première cause de cécité incurable mais évitable. Il représente plus de 14% de l’ensemble des cécités. Dans le but de décrire un état des lieux du glaucome au Maroc, une enquête a été réalisée auprès de l’association marocaine contre le glaucome, de l’industrie pharmaceutique, ainsi que des orthoptistes. Les différents facteurs de risque du glaucome, qu’ils soient de prédisposition, d’apparition ou d’aggravation, sont abordés, avec leurs particularités propres à notre pays et notre contexte socio-économique. Des problématiques posées par cette maladie complexe sont exposées. Elles sont à l’origine d’une prise en charge des patients bien inférieure à nos espérances. En effet, à peine 10 à 15% des patients sont dépistés et traités sur les 500 000 à 700 000 glaucomateux estimés. Par ailleurs, on note que sur un maximum de 72850 patients traités pour glaucome chronique, moins de 20% ont eu un relevé de champ visuel dans l’année.Le but de toute action dans le domaine du glaucome, devra s’appuyer sur des indicateurs fiables, avec pour objectif de sensibiliser sur de mauvaises habitudes concernant certaines ONG, certains pharmaciens et certains opticiens, et certaines contraintes liées à la qualité de la relation médecin -patient et par conséquent la qualité de vie du patient atteint de glaucome. L’amélioration de nos performances repose sur l’implication de tous les intervenants dans le domaine de la santé : pouvoirs publics, corps médical et société civile

    Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (mertensiothuria) leucospilota collected from Malaysian waters = Populasi Mikrob dalam Cecair Selom Stichopus chloronotus dan Holothuria (Mertensiothuria) leucospilota dari Perairan Malaysia

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    This preliminary study aimed to isolate and identify microbes that inhabit the coelomic fluid of two local species of sea cucumbers collected from Malaysian waters - a ‘gamat’ species i.e. Stichopus chloronotus Brandt, 1835 and the most abundant ‘timun laut’ species in Malaysia i.e. Holothuria (Mertensiothuria) leucospilota (Brandt 1835). Phylogenetic analyses of partial 16S rRNA mtDNA gene sequences suggested the presence of at least eight microbial genera i.e. five bacterial genera - Bacillus, Exiguobacterium, Pseudomonas, Stenotrophomonas and Vibrio - isolated from the coelomic fluid of H. leucospilota and three genera of gram-positive bacteria from the Micrococcaceae family - Kytococcus, Micrococcus and either Kocuria or Rothia - isolated from the coelomic fluid of S. chloronotus. We speculate that less diverse microbial population in S. chloronotus as compared to H. leucospilota could be due to a number of environmental factors e.g. penetration of light surrounding the habitats of both species, the feeding behaviour of H. leucospilota and the higher level of antimicrobial properties of coelomic fluid in S. chloronotus. In terms of antimicrobial-resistance capability test, an isolate from genus Pseudomonas that is suspected to be P. alcaligenes exhibited high resistance towards streptomycin. Another isolate from genus Stenotrophomonas that was suspected to be S. maltophilia showed moderate resistance towards streptomycin and lower resistance towards kanamycin. Both isolates were from the coelomic fluid of H. leucospilota. Tetracycline inhibited the growth of all bacterial isolates tested. Further studies with more specimens of S. choronotus and H. leucospilota from broader geographical locations and the use of complete mtDNA genes along with morphological approaches for species identification may facilitate to provide better insights into the microbial population in the coelomic fluid of both local sea cucumber species

    Microbial population in the coelomic fluid of Stichopus chloronotus and Holothuria (Mertensiothuria) leucospilota collected from Malaysian waters

    No full text
    This preliminary study aimed to isolate and identify microbes that inhabit the coelomic fluid of two local species of sea cucumbers collected from Malaysian waters - a 'gamat' species i.e. Stichopus chloronotus Brandt, 1835 and the most abundant 'timun laut' species in Malaysia i.e. Holothuria (Mertensiothuria) leucospilota (Brandt 1835). Phylogenetic analyses of partial 16S rRNA mtDNA gene sequences suggested the presence of at least eight microbial genera i.e. five bacterial genera - Bacillus, Exiguobacterium, Pseudomonas, Steno trophomonas and Vibrio - isolated from the coelomic fluid of H. leucospilota and three genera of gram-positive bacteria from the Micrococcaceae family - Kytococcus, Micrococcus and either Kocuria or Rothia - isolated from the coelomic fluid of S. chloronotus. We speculate that less diverse microbial population in S. chloronotus as compared to H. leucospilota could be due to a number of environmental factors e.g. penetration of light surrounding the habitats of both species, the feeding behaviour of H. leucospilota and the higher level of antimicrobial properties of coelomic fluid in S. chloronotus. In terms of antimicrobial-resistance capability test, an isolate from genus Pseudomonas that is suspected to be P. alcaligenes exhibited high resistance towards streptomycin. Another isolate from genus Steno trophomonas that was suspected to be S. maltophilia showed moderate resistance towards streptomycin and lower resistance towards kanamycin. Both isolates were from the coelomic fluid of H. leucospilota. Tetracycline inhibited the growth of all bacterial isolates tested. Further studies with more specimens of S. choronotus and H. leucospilota from broader geographical locations and the use of complete mtDNA genes along with morphological approaches for species identification may facilitate to provide better insights into the microbial population in the coelomic fluid of both local sea cucumber species
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