31 research outputs found
BETWEEN ARTEMISININ AND DERIVATIVES WITH NEURAMINIDASE: A DOCKING STUDY INSIGHT
Get PDFObjectives: This study aims to find the relationship between artemisinins and neuraminidase (NA) with molecular docking study and also to determine the most potent NA inhibitor from artemisinin and derivatives.Methods: All ligands were sketched and optimized using Gaussian 03W with Hartree-Fock method basis sets 6-311G. Molecular docking was performed using AutoDock 4.2.3 toward NA in complexes with oseltamivir as co-crystal ligand. The main parameters used were the free energy of binding (ΔG) and dissociation constant (Ki) as affinity marker.Results: Artesunate provided most negative free ΔG and lowest Ki toward NA with −9.55 kcal/mol and 100.66 nM, respectively. Artesunate shows higher affinity than oseltamivir with interactions between artesunate and amino acids at position 246 had important influences on artesunate affinity toward NA from H5N1.Conclusion: In silico molecular docking results indicated that artesunate could be considered as NA inhibitor and should be potential to be developed as anti-influenza particularly to H5N1 with oseltamivir resistance.</jats:p
Uji Aktivitas Enzim Superoksida Dismutase dalam Ekstrak Mesokarp Buah Merah (Pandanus conoideus Lamarck) Menggunanakan Densitometri Citra Elektroforegram
Get PDFPenelitian ini bertujuan menguji aktivitas super oksida dismutase (SOD) mesokarp buah merah (Pandanus conoideus Lamarck). Pengujian dilakukan dengan metode elektroforesis gel poliakrilamid dan densitometri citra terhadap ekstrak kasar buah merah dan ekstrak hasil pemurnian dengan dialisis setelah pengendapan dengan amonium sulfat konsentrasi 25, 50 dan 75% b/v. Aktivitas SOD ditunjukkan dengan terbentuknya pita transparan pada gel setelah dua tahap proses pewarnaan yaitu dengan nitro biru tetrazolium dan riboflavin, dan hasil pencitraan gel dianalisis dengan densitometer. Aktivitas SOD ditentukan dengan membandingkan terhadap SOD dari Escherichia coli dan diperoleh aktivitas SOD sebesar 420 U/mL atau setara dengan 836 U/gram buah merah.Kata kunci: Elektroforesis, Densitometer, Superoksida dismutase, Buah merahThe aim of this study was to test the activity of SOD buah merah (Pandanus conoideus Lamarck). Evaluation was done by polyacrylamide gel electrophoresis and image densitometry was performed to raw extract of buah merah, and the extract pure by dialysis after precipitation with ammonium sulfate concentrations of 25, 50 and 75% w/v. SOD activity was shown by the formation of transparent tape on the gel after the gel staining by nitro blue tetrazolium and riboflavin, then imaging results were analyzed by densitometer. SOD activity was determined by comparing the SOD from Escherichia coli and it was 420 U/mL.Keywords: Electrophoresis, Densitometer, Superoxide dismutase, red frui
Penentuan Kadar Resistin dan Korelasinya dengan Beberapa Parameter Klinik pada Kondisi Normal Dan Kurang Tidur
Get PDFResistin merupakan suatu molekul yang berperan penting dalam patofisiologi beberapa penyakit seperti obesitas, diabetes mellitus dan aterosklerosis yang merupakan faktor resiko penyakit kardiovaskuler. Kadar resistin di dalam serum berperan dalam menghubungkan obesitas dengan diabetes dan resistensi insulin. Penelitian ini bertujuan untuk menentukan kadar resistin dan korelasinya dengan beberapa parameter klinik pada subyek normal dan kurang tidur. Diperoleh profil yang terdistribusi normal untuk hasil pemeriksaan dengan nilai p> 0,05. Hasilnya tidak ada korelasi antara konsentrasi serum resistin dengan beberapa parameter klinik yang diperiksa tetapiterdapat perbedaan hasil pemeriksaan antara subyek tidur normal dengan kurang tidur pada semua parameter, namun yang berbeda signifikanhanya konsentrasi resistin dengan p=0,010.Kata kunci: resistin, subyek normal, kurang tidur.AbstractResistin is a molecule that plays an important role in several pathophysiological conditions such us obesitas, diabetes mellitus and aterosklerosis is a risk factor cardiovascular diseases. The level of resistin in serum plays a role in connecting obesity to diabetes and insulin resistance. The aims of this study was to determine serum resistin levels andtheir correlation to the several clinical parameters in normal subjects and sleep-deprived subject. A profil normal distribution was retrived for the result of examination, with a value of p> 0.05. The result showed that there was no significant correlation between serum resistin concentrations with several examined clinical parameters but there are differences in examination results between subjects with normal sleep and sleep deprivation on all parameters, but the significant difference was only parameter of resistin concentrations with p= 0.010.Keywords: resistin, normal subject,sleep deprivation
Korelasi Konsentrasi Serum HS-CRP, Adiponektin dan Cystatin-c dengan LDL Teroksidasi sebagai Petanda Aterosklerosis pada Pria Sindroma Metabolik
No full textTelah dilakukan uji korelasi antara konsentrasi serum hs-CRP, Adiponektin dan Cystatin-C dengan LDL teroksidasi sebagai petanda aterosklerosis pada pria sindroma metabolik. Hasil penelitian menunjukkan terdapat korelasi konsentrasi serum antara hs-CRP dengan LDL teroksidasi dengan nilai r=0,316 dan p=0,022, korelasi konsentrasi serum Adiponektin dengan LDL teroksidasi dengan nilai r= -0,177 dan p=0,046 dan korelasi konsentrasi serum Cystatin-C dengan LDL teroksidasi dengan nilai r= 0,317 dan p= 0,022.Kata kunci : Sindroma Metabolik, hs-CRP, Adiponektin, Cystatin-CAbstractThe correlation test has been conducted between serum concentration of hs-CRP, Adiponectin and Cystatin-C with oxidized LDL as a marker of Atherosclerosis in male metabolic syndrome. The other research result has shown there was a correlation between the serum concentration of hs-CRP with oxidized LDL by value of r = 0.316 and p = 0.022, correlation of serum concentration of oxidized LDL Adiponectin by value of r = -0.177 and p = 0.046 and the correlation of serum concentration of Cystatin-C with oxidized LDL by value of r = 0.317 and p = 0.022.Keywords : Metabolic Syndrome, hs-CRP, Adiponectin, Cystatin-
Biphasic kinetics of inositol 1,4,5-trisphosphate accumulation, in gastrin-stimulated parietal cells Effects of pertussis toxin and extracellular calcium
No full textAbstractThe effect of Pertussis toxin (PTx) and extracellular Ca2+ were investigated on gastrin-induced Ins(1,4,5)P3 mass level in isolated gastric parietal cells. Basal Ins(1,4,5)P3 content was 5.48±0.49 pmol/500 000 cells. Gastrin (10 nM) induced a rapid increase in Ins(1,4,5)P3 content which was maximal after 15 s and corresponded to 2–2.5-fold basal level; this Ins(1,4,5)P3 content then decreased within 30 s. After a longer time of gastrin exposure (> 1 min), a sustained and unexpected increased in Ins(1,4,5)P3 accumulation was observed which was maximal at 7.5 min (corresponding to 2.3–2.8-fold basal value) and slightly decreased thereafter. PTx treatment of cells (200 ng/ml) for 3 h or removal extracellular Ca2+ did not affect the rapid rise, but drastically reduced the sustained increase in Ins(1,4,5)P3 content (60–100% inhibition); this inhibition was not evident after 10 min of hormone stimulation. Furthermore, diltiazem, a Ca2+ channel blocker, led to a similar inhibition of the sustained increase. We concluded that: (i) gastrin induced rapid increase in Ins(1,4,5)P3 content via a mechanism insensitive to PTx and to extracellular Ca2+, and (ii) gastrin induced a sustained increase in Ins(1,4,5)P3 level via a mechanism sensitive to PTx and to extracellular Ca2+. Even though the rapid rise in Ins(1,4,5)P3 content may be involved in the intracellular Ca2+ mobilization occurring after the first seconds of hormone stimulation, the physiological role of the sustained Ins(1,4,5)P3 increased level remains to be elucidated
CORRELATION STUDY OF SERUM CALCIUM LEVELS AND SERUM CARTILAGE OLIGOMERIC MATRIX PROTEIN LEVELS IN RHEUMATOID ARTHRITIS PATIENTS IN BANDUNG, INDONESIA
Get PDF  Objective: This study was designed is to determine the correlation between serum calcium level and serum cartilage oligomeric matrix protein (COMP) level in Indonesian patients with rheumatoid arthritis (RA).Subjects and Methods: The subjects are patients who visit the rheumatology clinic at a government hospital in Bandung, Indonesia. Serum calcium level was determined by a complexometric method, and the serum COMP level was determined by enzyme-linked immunosorbent assay. Statistical analysis was performed with R software.Results: The serum calcium level was 82.24±44.82 mg/mL in RA patients and 99.04±60.56 mg/mL in normal controls. The serum COMP level was 843.80±35.79 ng/ml in RA patients and 830.00±48.92 ng/ml in normal controls.Conclusion: There is no correlation between serum calcium level and serum COMP levels in RA patients (p=0.967 and rho=0.006)
Optimasi Isolasi dan Karakterisasi Jakalin dari Biji Nangka
Get PDFTelah diisolasi jakalin dari biji nangka (Artocarpus heterophyllus) menggunakan metode kromatografi afinitas dengan matriks guar gum yang telah dipaut silang dengan epiklorohidrin, isolasi dilakukan dengan menggunakan pengelusi D-galaktosa. Hasil karakterisasi jakalin menggunakan SDS-PAGE menunjukkan bahwa jakalin memiliki dua pita dengan bobot molekul 14,1 dan 15,5 kDa, antar subunitnya tidak dihubungkan dengan ikatan disulfida. Jakalin yang diisolasi memiliki kemampuan mengaglutinasi eritrosit, kemampuan hemaglutinasi jakalin tidak berkurang setelah diinkubasi pada suhu 20oC dan 30oC, tetapi setelah inkubasi pada suhu 40oC, 50oC, 60oC, 70oC, aktivitasnya turun masing-masing 75%, 87,5%, 93,75% dan 98,4%. Kemampuan hemaglutinasi jakalin tidak berkurang setelah diinkubasi pada pH 5, 6, dan 7, tetapi aktivitasnya turun 75% setelah inkubasi pada pH 2, 3, 4, dan 8, serta turun 96,9% pada pH 9 dan 10. Kemampuan hemaglutinasi jakalin dihambat oleh D-galaktosa dengan kemampuan inhibisi hemaglutinasi sebesar 6,25 mM, aktivitas hemaglutinasi jakalin tidak dihambat oleh D-manosa, D-glukosa, fruktosa, laktosa, arabinosa, maltosa, dan manitol. Diperoleh jakalin dengan perolehan rata-rata sebesar 0,32% b/b dari serbuk kering biji nangka.Kata kunci: Jakalin, lektin, Artocarpus heterophyllus, D-galaktosa.Jacalin from jackfruit (Artocarpus heterophyllus) had been isolated using affinity chromatography method with epichlorohydrin crosslinked guar gum as the matrix, isolation carried out using D-galactose as the eluen. Characterizationjacalin using SDS-PAGE showed that jacalin has two bands with molecular weights 14.1 and 15.5 kDa, intersubunit not connected with disulfide bonds. Jacalin isolates have the ability to haemagglutination erythrocytes, haemagglutination activity of jacalin maintained after incubation at 20oC and 30oC, but the activity decreased at 40, 50, 60, and 70 oC of incubation, which were 75%, 87.5%, 93.75% and 98.4% , respectively. Jacalin hemagglutination ability is not reduced after incubation at pH 5, 6, and 7, but the activity down 75% after incubation at pH 2, 3, 4, and 8, and down 96.9% at pH 9 and 10. The haemagglutination activity of jacalin was inhibited by D-galactose with the capacity inhibition value was 6.25 mM, but it was not inhibited by D-manose, D-glucose, fructose, lactose, arabinose, maltose, and manitol. Average recovery of jacalin is 0.32% w/w of jackfruit dry powder.Keywords: Jacalin, lectin, Artocarpus heterophyllus, D-galactose
CORRELATION STUDY OF SERUM CALCIUM LEVELS AND SERUM CARTILAGE OLIGOMERIC MATRIX PROTEIN LEVELS IN RHEUMATOID ARTHRITIS PATIENTS IN BANDUNG, INDONESIA
No full text Objective: This study was designed is to determine the correlation between serum calcium level and serum cartilage oligomeric matrix protein (COMP) level in Indonesian patients with rheumatoid arthritis (RA).Subjects and Methods: The subjects are patients who visit the rheumatology clinic at a government hospital in Bandung, Indonesia. Serum calcium level was determined by a complexometric method, and the serum COMP level was determined by enzyme-linked immunosorbent assay. Statistical analysis was performed with R software.Results: The serum calcium level was 82.24±44.82 mg/mL in RA patients and 99.04±60.56 mg/mL in normal controls. The serum COMP level was 843.80±35.79 ng/ml in RA patients and 830.00±48.92 ng/ml in normal controls.Conclusion: There is no correlation between serum calcium level and serum COMP levels in RA patients (p=0.967 and rho=0.006).</jats:p
Synthesis and Characterization of 2-monoacylglycerols from Canarium Oil (Canarium Indicum)
No full textAbstractThis paper studies synthesis and characterization of 2-monoacylglycerols (2-MAGs), generally used for structured lipid production. Synthesis used canarium oil by enzymatic reaction using immobilized lipase from Mucor miehei that has a specific activity on the sn-1 and 3 of the triacylglycerol structure. Characterization of 2-MAGs by thin layer chromatography using a standard of 2-oleoylglycerol followed by isolation and purification to determine the fatty acids composition.The highest composition of fatty acids on 2-MAGs were unsaturated fatty acids, namely oleic acid (939.8ppm) and linoleic acid (445.5ppm), therefore it could be used for the synthesis of structured lipid
Transformasi Plasmid pTRLI ke dalam Protoplas Aspergillus terreus dengan Penambahan Polietilenglikol
No full textPlasmid transformation is the introduction and incorporation of exogenous plasmid into cells or protoplast. The purpose of this research is transformation of pTRLI plasmid into protoplasts of Aspergillus terreus and obtain stable transformants. The research was initiated by isolation of pTRLI plasmid and determining its purity and concentration by nanodrop machine. Furthermore, protoplasts of A. terreus were isolated enzymatically by addition of chitinase, cellulase, and maserozyme enzymes. pTRLI plasmid was transformed into protoplasts of A. terreus by addition of calcium chloride and polyethyleneglycol (PEG) solutions. These transformants were grown in Czapek-Dox agar medium containing pyrithiamine 1 mg/L and the number of transformants/µg of pTRLI plasmid was calculated. The number of transformants were produced ranging from 12 to 19 transformants/µg of pTRLI plasmid. The success of the transformation was indicated by ptrA gene in transformants that could be amplified by PCR of 801 base pairs in size. It was concluded that PEG solution could be used to transform pTRLI plasmid into protoplasts of A. terreus and transformants are stable up to live generations by growing the transformants in Czapek-Dox agar medium containing pyrithiamine 1 mg/L.Transformasi plasmid merupakan proses masuknya plasmid ke dalam protoplas atau sel inang yang mengakibatkan perubahan materi genetika. Tujuan dari penelitian untuk mentransformasi plasmid pTRLI ke dalam protoplas Aspergillus terreus dan menentukan stabilitas transforman. Penelitian diawali dengan isolasi plasmid pTRLI, penentuan kemurnian dan konsentrasi plasmid pTRLI dengan menggunakan serapan pada panjang gelombang 260 dan 280 nm dari alat nanodrop. Kemudian, protoplas A. terreus diisolasi secara enzimatik dengan enzim kitinase, selulase, dan maserosim. pTRLI ditransformasi ke dalam protoplas A. terreus dengan penambahan kalsium klorida dan polietilenglikol. Transforman ditumbuhkan dalam media Czapek Dox agar yang mengandung piritiamin 1 mg/L. Jumlah transforman yang dapat tumbuh antara 12 sampai 19 transforman/µg plasmid pTRLI. Transforman dideteksi dengan mengamplilikasi gen ptrA yang berukuran 801 pb. Dari penelitian ini, dapat disimpulkan bahwa PEG dapat digunakan untuk mentransformasi plasmid pTRLI ke dalam protoplas A. terreus dan transforman stabil sampai generasi ke S
