1,720,953 research outputs found
Genetic analysis and hybrid prediction in tropical maize (Zea mays L.) using phenotypic and single nucleotide polymorphic markers.
No full textDoctoral Degree. University of KwaZulu-Natal, PietermaritzburgMaize (Zea mays L., 2n = 2x = 20) is a commodity crop serving the food, feed, and processing industries globally. The productivity of maize in Africa remains low (< 2 t/ha) due to various yieldlimiting factors, including abiotic stresses (such as drought, heat stress, flooding, waterlogging, erosion and poor soil health), and biotic stresses (e.g. foliar diseases and insect pests). Limited adoption of new high yielding varieties, slow rate of varietal turnover , socio-economic constraints, and policy issues further hinder productivity. Seed Co Limited is a Pan-African seed company involved in the research, development, and commercialization of seeds of major food security grain crops, including maize. The Seed Co breeding program aims to enhance the yields of new generation maize cultivars via hybrid breeding by utilizing complementary and contrasting inbred lines. New lines and experimental hybrids are developed and phenotyped using economic agronomic traits and genotyped using high-resolution Single Nucleotide Polymorphism (SNP) markers to facilitate effective selection. Integrating phenotypic and genomic selection accelerates the development of inbred lines with desirable traits to create high-performing single crosses and three-way hybrids. The new hybrids should undergo rigorous field testing for yield gains and stability across various locations to guide cultivar release and commercialization. Therefore, to complement this breeding initiative, the objectives of the study were: to assess a maize germplasm panel's genetic diversity and population structure comprising 182 founder lines and 866 derived inbred lines using Single Nucleotide Polymorphism (SNP) markers to identify genetically unique lines for hybrid breeding, to conduct genome-wide prediction of yield and component traits using qualitative and quantitative phenotypic traits and SNP markers based on the additive-dominant genomic best linear unbiased predictions model to compute genomic estimated breeding values and genomic estimated genetic values to guide inbred line development and hybrid breeding, to assess the gains in yield and yield components among single cross maize hybrids selected through genomic prediction across representative locations to guide breeding and production and to determine the combining ability effects of newly selected inbred lines and quantify the magnitude of heterosis and genotype by environmental interaction (GEI) effects of single cross hybrids to select and recommend contrasting elite lines and experimental hybrids.
In the first study, 182 founder and 866 derived maize inbred lines were characterized for genetic diversity and population structure analyses using SNP markers to identify genetically unique lines for hybrid breeding through beneficial allelic combinations. Genotyping was performed using the Affymetrix platform for the 182 founder lines (1201 SNP markers) and the Midseq platform for the 866 derived lines (1484 markers). Moderate genetic variation with genetic distance ranging from 0.004 to 0.44 (mean: 0.25) for founder lines and 0.004 to 0.34 (mean: 0.13) for derived lines was observed. Heterozygosity values ranged from 0.00 to 0.24 for both lines. About 82% of the 1201 markers and 84% of the 1484 markers exhibited polymorphism information content ranging from 0.25 to 0.50, detecting a high level of genetic diversity and that the SNPs were highly informative in distinguishing the tested lines. Analysis of molecular variance revealed significant genetic differences (P ≤ 0.001) among and within populations in the founder and derived lines. Notably, within-population variations accounted for 97% (founder lines) and 88.38% (derived lines) of the detected variations. Population structure analysis identified three subpopulations among founder lines and two among derived lines, which was supported by cluster analysis. Based on pairwise comparisons, genetically distant lines were selected, including G15NL337 and G15NL312 (Cluster 1), 15ARG152 and RGS-PL44 (Cluster 2), RGS-PL44 and 15ARG149 (Cluster 2), and RGS-PL33 and RGS-PL44 (Cluster 2). The selected lines are genetically distinct and recommended for marker-assisted hybrid maize breeding to leverage beneficial alleles.
The second study genotyped 1,102 genetically diverse inbred lines from two heterotic groups (N3 and SC) using high-density SNP markers. The 1,102 lines and 4 testers were crossed in a line-by-tester design to generate 2,830 single cross hybrids (SCHs). Phenotypic data were collected from field trials with the following SCHs: 684 evaluated at five locations in 2018/19, 760 at four locations (2019/20), 646 at four locations (2020/21), and 740 at four locations (2021/22) summer seasons in Zimbabwe. The trials were laid out in a 6 x 7 alpha lattice design with two replications at each site. 20 highperforming and contrasting inbred lines with the highest genomic estimated breeding values (GEBVs) and genomic estimated genetic values (GEGVs), each from the two heterotic groups, were identified for genetic advancement, combining ability tests and commercial hybrid development. 20 highperforming candidate SCHs with high GEGVs were identified for three-way hybrid development, variety registration and commercialization.
In the third study, 30 SCHs were developed from 11 inbred lines (6 from the N3 group and 5 from the SC group) with the highest predicted GEGVs for grain yield and associated traits using the genotypic best linear unbiased prediction (GBLUP) model. The lines were crossed using a factorial mating design with the six N3 lines used as female and five SC lines as male. The derived 30 SCHs and six commercial single cross check hybrids were field evaluated in seven locations, four in Zimbabwe and three in Zambia using a 6 x 6 alpha lattice design with two replications at each location. A combined analysis of variance revealed significant (P≤0.05) variation among the hybrids for the assessed 11 quantitative traits. Significant yield gains were realized over the mean of checks (at 13.09%), mean of the population (10.83%) and mean of best check (1.47%). Moderate to high broad-sense heritability (50 to 94%) and genetic advance were recorded for most of the assessed traits, indicating the success of selection assisted by genomic predictions. The study identified three best single cross hybrids (i.e., CTL03 x G16NL721, CTL03 x G17NL544 and GS-PL07 x G17NL544) with high and stable yields and recommended for commercialization.
In the fourth study, 11 elite inbred lines (6 female parents from N3 and 5 male parents from SC group) were crossed using a factorial mating design, resulting in 30 SCHs. The lines were selected based on the highest GEGVs for yield and component traits through GS using the GBLUP model. The 30 SCHs and six commercial check hybrids were field evaluated at seven locations (four in Zimbabwe and three in Zambia) during the 2022/2023 summer season. The trials were arranged in a 6 x 6 alpha lattice design with two replications at each location. Data were recorded on yield and yield components, and general combining ability (GCA) and specific combining ability (SCA) effects were computed. Significant GCA effects for grain yield (GY) were noted for lines CTL03, G17NL544, G16NL721, and GS-PL07, while significant SCA effects were recorded for crosses 15AG163 x G16NL679, G15NL304 x G17NL642, and 15AG162 x G16NL679. The additive main effects and multiplicative interaction (AMMI) model explained 38.95%, 50.58% and 7.24% of the total variation in GY due to genotype (G), environment (E), and genotype x environment interaction (GEI) effects in that order. The test locations were clustered into two mega environments: Rattray Arnold Research Station (RARS), Agricultural Research Trust (ART), Mpongwe Research Station (MPRS), and Lusaka West Research Station (LWRS) (Environment 1), and Mkushi Research Station (MKRS), Stapleford Research Centre (STAP), and Kadoma Research Centre (KRC) (Environment 2). The genotype and genotype-by-environment interaction (GGE) biplot analysis identified hybrids G15NL304 x G17NL544 and 15AG162 x G17NL544 as high-yielding and stable, suitable for commercialization. The two mega-environments and the selected stable, high-yielding general and specific combiners are recommended for genotype evaluation and production in Zimbabwe, Zambia, and comparable agroecologies.
Overall, the present study identified contrasting and genetically delineated inbred lines and enhanced the existing heterotic groups using high-throughput SNP markers. Best-performing lines (e.g. CTL03 and GS-PL07) were selected from the N3 heterotic group and G17NL544 and G16NL721 from the SC heterotic group. New single cross hybrids, such as CTL03 x G16NL721, CTL03 x G17NL544, and GS-PL07 x G17NL544, were selected with grain yields of 8.38 t/ha, 8.24 t/ha, and 8.23 t/ha, respectively. The new experimental hybrids are recommended for three-way hybrid development or release following multi-environment evaluation
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
Author Under Sail The Imagination of Jack London, 1893-1902
No full textIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Intro -- Title Page -- Copyright Page -- Dedication -- Contents -- Acknowledgments -- Introduction -- 1. Spirit Truth -- 2. From Absorption to Theatricality and Back Again -- 3. "I Will Build a New Present" -- 4. Sons as Authors -- 5. Fathers as Publishers -- 6. The Daughter as Author -- 7. Lovers as Authors -- 8. At Sea with the Family -- 9. Yellow News, Yellow Stories -- 10. The Return Home -- Notes -- Bibliography -- Index -- About Jay WilliamsIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
- …
