1,721,025 research outputs found
Glycosylation and antigenic properties of Phlebotomus perniciosus and P. orientalis salivary proteins
Full text linkThe goal of this study was to map the glycosylation pattern and antigenic properties of the salivary proteins of two closely related sand fly species, Phlebotomus perniciosus and P. orientalis. Affinity blotting with commercially available lectins revealed that many salivary proteins of these species are N-glycosylated, while the presence of O-glycosylation could not be confirmed. The level of N-glycosylation of most of these proteins is quite low, a larger number of potential N-glycosylation sites were found only in the amino acid sequences of P. orientalis hyaluronidase and endonucleases of both species tested. Four antigens from P. perniciosus salivary glands were selected for expression in a bacterial expression system; two of these proteins (PpeSP01 and PpeSP01B) were not glycosylated and the glycosylation level of the remaining two (PpeSP03B and PpeSP07) was low. The antigenic properties of the four chosen recombinant proteins were subsequently tested using immunoblot and ELISA. During the initial experiments with the sera of dogs experimentally bitten by P. perniciosus, two proteins (rSP07 and rSP01B) were proven unsuitable and they were excluded from further experiments. Recombinant proteins rSP03B and rSP01 were recognized by the same IgG antibodies as the native forms of these proteins..
Glycosylation, Ultrastructure and Antigens of the surface of larval Trematodes
No full textKatedra parazitologieDepartment of ParasitologyPřírodovědecká fakultaFaculty of Scienc
Prvoci rodu Leishmania (Kinetoplastida: Trypanosomatidae): interakce s přenašeči a hostiteli.
Full text linkInteraction Phlebotomus - Leishmania
No full textKatedra parazitologieDepartment of ParasitologyPřírodovědecká fakultaFaculty of Scienc
Borrelia burgdorferi sensu lato a Anaplasma phagocytophilum v České republice
No full textShrnutí Cílem této práce bylo studium výskytu dvou klíšt'aty přenosných bakterií, Borrelia burgdorferi sensu lato a Anaplasma phagocytophilum, u klíšt'at a divokých a domácích zvířat v České republice. Zjistili jsme, že prevalence obou baterií u klíšt'at byla přibližně shodná. Hlodavci byli ve většině případů infikováni B. afzelii. Výskyt B. burgdorferi s. s. u hlodavců byl také poměrně častý. U norníka rudého jsme zjistili statisticky vyšší prevalenci B. burgdorferi s. l. než u myšice lesní či myšice křovinné. Naopak séroprevalence anti-Borrelia protilátek byla vyšší u obou myšic v porovnání s norníkem rudým. Výskyt A. phagocytophilum byl pozorován ve vyšším procentu u vysoké zvěře a zajíců než u lišek a divokých prasat, u kterých byla Anaplasma detekována jen v malém počtu případů. Pozorovali jsme rovněž podobnou prevalenci anaplasmózy u všech testovaných domácích zvířat. U symptomatických psů byla vyšší pravděpodobnost, že jsou infikováni A. phagocytophilum než u asymptomatických psů. Naše nálezy ukazují, že výskyt obou bakterií, B. burgdorferi s. l. a A. phagocytophilum, je u vektorů, rezervoárových hostitelů i dalších hostitelů v České republice častý. V této práci jsou rovněž...The aim of this thesis was to assess the occurrence of two tick- borne bacteria, Borrelia burgdorferi sensu lato and Anaplasma pha- gocytophilum, in ticks, wild and domestic animals in the Czech Republic. In ticks, similar prevalence of both bacteria was ob- served. In rodents, the majority of infections were caused by B. afzelii while the infection with B. burgdorferi s. s. was also quite frequent. Infection with B. burgdorferi s. l. was more common in bank voles than in wood or yellow-necked mice. The prevalence of anti-Borrelia antibodies was higher in wood or yellow-necked mice than in bank voles. A. phagocytophilum was in a higher percentage of cases in the deer family and hares as compared to foxes and boars. We observed a similar prevalence of anaplasmosis in all domestic animals tested. We demonstrated that symptomatic dogs had a higher chance to be infected with A. phagocytophilum than asymptomatic dogs. Our findings suggest that the exposure to B. burgdorferi s. l. and A. phagocytophilum is common in vectors, reservoirs and hosts in the Czech Republic. Molecular and serological techniques for detection of these pathogens are also described in this thesis, including conven- tional PCR, nested PCR, real-time PCR with DNA quantification and melting curve analysis, RFLP analysis of the 5S-23S...Department of Genetics and MicrobiologyKatedra genetiky a mikrobiologieFaculty of SciencePřírodovědecká fakult
Molekulární prognostické a prediktivní markery u kolorektálního karcinomu
No full textin English Colorectal cancer is currently one of the three major causes of cancer-related death. In order to help clinicians to treat colorectal cancer, it is necessary to introduce more effective tools that will improve not only early diagnosis, but also prediction of the most likely progression of the disease and response to chemotherapy. This thesis aims to describe the most accepted biomarkers among those proposed for use in CRC divided based on the clinical specimen that is examined (tissue, feces or blood) along with their restrictions. In relation to my research, the thesis will also focus on alternative splicing that has emerged as an important regulator and potential treatment target in CRC. Alterations in gene expression leading to colorectal carcinogenesis results in dysregulated levels of nucleic acids and proteins, which can be utilized for the identification of modern, minimally invasive molecular biomarkers. One of the alternative splicing factors are MBNL proteins. The goal of my work that will be presented here was to analyze gene expression of the MBNL family of regulators of alternative splicing in various stages of colorectal cancer development, together with the MBNL-target splicing events in FOXP1 and EPB41L3 genes and tumor-related CD44 variants. The study was done using...in Czech Kolorektální karcinom je v současné době jednou ze tří hlavních příčin úmrtí souvisejících s nádorovými onemocněními. Aby se klinickým lékařům pomohlo léčit kolorektální karcinom, je nutné zavést účinnější nástroje, které zlepší nejen včasnou diagnostiku, ale také predikci nejpravděpodobnější progrese onemocnění a reakce na chemoterapii. Tato práce si klade za cíl popsat nejpřijatelnější biomarkery mezi těmi, které jsou navrženy pro použití v CRC, rozdělené podle klinického vzorku, který je vyšetřován (tkáň, stolice nebo krev), spolu s jejich omezeními. Ve vztahu k mému výzkumu se práce zaměří také na alternativní sestřih, který se ukázal jako důležitý regulátor a potenciální cíl léčby v CRC. Změny v genové expresi vedoucí ke kolorektální karcinogenezi vedou k dysregulovaným hladinám nukleových kyselin a proteinů, které lze využít k identifikaci moderních, minimálně invazivních molekulárních biomarkerů. Jedním z alternativních spojovacích faktorů jsou MBNL proteiny. Cílem mé práce, která zde bude představena, byla analýza genové exprese rodiny MBNL regulátorů alternativního sestřihu v různých fázích vývoje kolorektálního karcinomu, spolu s událostmi sestřihu cíle MBNL v genech FOXP1 a EPB41L3 a CD44 souvisejícími s nádorem varianty. Studie byla provedena pomocí vzorků nádorové tkáně a...Ústav histologie a embryologieFaculty of Medicine in PilsenLékařská fakulta v Plzn
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Pathogenic Effect of Avian Leukosis Viruses in Chicken
No full textcomparativestudyof pďhomorphologicalchangesin lymphoidtissuesinducedby ALVs of different subgroupspecificity, hasnot beenperformed.Usingthe chicken model we aimed at clarification of additional features,especially pathomorphologicaland immunological chara- cteristics of the wasting diseaseinducedby different strainsof ALV{. In orderto achievea propercompďison,alsoadditionalALV subgroup(A'B'D) wereincluded. on the otlrerhand, several retroviruseshave been found to induce kidney fumoÍs. MAv2 (N) is an avian nonacuteoncogenicÍetrovirusof subgroupB ALv. In chickens infec- ted in ovo or early afrerhatchingit induces,with high efÍrciency'multip|eclonal embryonic- type tumorsof kidney - nephnoblastomas(Wattsand Smith, 1980).The model of MAV2 (N)- induced chicken nephroblastomais basedon the assumedabiliý of MAV2(N) retrovirusto transformcells by insertionalmutagenesis,thatis, by a deregulationof expressionof geneshit by the proviral integration.This avian model ofnephroblastomarepresentsa valuabletool for identi$ing genesinvolved in themaligrant transformationof cells. It is assumedthatmacro- scopic nepfuoblastomasarise by clonal expansion of blastema cells in which MAV2 (N) provirus has deregulatedspecific genescontroling differenciation and proliferation @ajer et ď.' 2003). Summary ALVs have a large potential to..
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