769 research outputs found
sj-docx-1-ahd-10.1177_00914150221106075 - Supplemental material for Fear of Incompetence in Family Caregivers and Dementia Care Transitions
No full textSupplemental material, sj-docx-1-ahd-10.1177_00914150221106075 for Fear of Incompetence
in Family Caregivers and Dementia Care Transitions by Anca M. Miron, Christopher L. Groves, Ashley E. Thompson, Susan H. McFadden, Haley R. Bowers and Jordyn M. DeBraal in The International Journal of Aging and Human Development</p
Iron responsive cell surface proteins in Thalassiosira pseudonana as probed by biotinylation and mass spectrometry
No full textWe investigated the cell-surface enriched proteome of Thalassiosira pseudonana under growth rate limiting (60-70% µ-max) and replete Fe conditions to better understand transporters that may be involved in Fe uptake. High and low Fe cultures were grown in the presence of 15N-NO3- (>98%) and 14N-NO3- (natural abundance), respectively, enabling relative quantification of proteins. In an effort to identify cell surface proteins, a cell surface labeling and enrichment method was developed and tested. Briefly, cell surface proteins were labeled with a free-amine reactive biotinylation reagent, soluble proteins were removed by membrane lysis and centrifugation, and biotinylated proteins were enriched on a neutravidin resin. Optimal conditions were sought for each of these three processes to increase coverage of cell surface labeled proteins. After elution, extracts were subjected to SDS-PAGE, in-gel tryptic digestion, and separation via liquid chromatography before identification and quantification by tandem mass spectrometry. Identification of cell surface proteins proved problematic due to biotinylation of some intracellular proteins and differential typtic digestion from the presence of the biotin linker arm. We obtained a greater than two-fold increase in abundance of the plasma membrane iron (III) permease (FTR1) under low Fe. A second FTR homolog was identified, indicating the presence of multiple Fe uptake pathways.M.S.Includes bibliographical referencesIncludes vitaby Ashley M. Ne
PETCT Imaging of Unstable Carotid Plaque with Ga-68 labelled Somatostatin Receptor Ligand.
No full textBACKGROUND
Ga68 labelled somatostatin receptor ligand PET imaging has recently been shown in preclinical and early human studies to have a potential role in the evaluation of vulnerable arterial plaques. We prospectively evaluated carotid plaque Ga68-DOTATATE uptake in patients with recent carotid events, assessed inter- and intra- observer variability of such measurements, and explored the mechanism of any plaque DOTATATE activity with immunohistochemistry in resected specimens.
MATERIALS & METHODS
20 consecutively consenting patients with recent symptomatic carotid events (transient ischaemic attack [TIA], stroke or amaurosis fugax), due for carotid endarterectomy were prospectively recruited. Ga68-DOTATATE PET/CT of the neck was performed prior to surgery. Ga68-DOTATATE uptake was measured by drawing regions of interest (ROI) along the carotid plaques and contralateral plaques/carotid arteries by experienced radionuclide radiologist and radiographer. Two PET quantification methods with inter- and Intra-observer variability were assessed. Resected carotid plaques were retrieved for sst-2 immunohistochemical stain.
RESULTS
Median time delay between research PET and surgery was 2days. SUV and TBR values for the symptomatic plaques and the asymptomatic contralateral carotid arteries/plaques show no significant difference (n = 19, p-value >0.10), regardless of quantification method. Intraclass correlation coefficient was >0.8 in all measures of carotid artery/plaque uptake (SUV) and >0.6 in almost all measures of target-to-background ratio (TBR). None of the excised plaques were shown to contain cells (macrophages, lymphocytes, vessel-associated cells) expressing sst2 on their cell membrane.
CONCLUSION
Ga68 DOTATATE activity on PET in recently symptomatic carotid plaques is not significantly different to contralateral carotids/plaques. Any activity seen on PET is not shown to be from specific sst2 receptor-mediated uptake in-vitro. It is therefore unlikely that sst2 PET/CT imaging will have a role in the detection and characterization of symptomatic carotid plaques
Estimating water requirements for hard red spring wheat for final irrigations
No full textBulletin no. 833 Moscow, Idaho :University of Idaho, College of Agriculture, Cooperative Extension System, 2001-05-01. Author(s): Ashley, Roger O.; Robertson, Larry D.; Seyedbagheri, Mir M.; Hopkins, Ivan C
Integrated sequence stratigraphy of the Paleocene-Lowermost Eocene, New Jersey coastal plain: implications for eustatic and paleoceanographic change
No full textThis study uses a high-resolution integrated sequence stratigraphic method to determine the paleoecologic, paleoceanographic, and sea-level changes during the Paleocene-early Eocene. One of the more intriguing problems in paleoceanography is determining the cause of large sea-level changes of > 20 m in < 1 Myr during the "greenhouse" interval. The principal hypotheses presented here suggest a glacioeustatic mechanism for sea-level change during the Paleocene-earliest Eocene. A multidisciplinary approach was implemented utilizing sequence stratigraphic core description, carbon and oxygen isotope analysis, benthic foraminiferal biofacies, and biostratigraphy (nannofossils) to evaluate the magnitude of sea-level changes and possible eustatic mechanisms. The bulk of this study was conducted on the New Jersey coastal plain because of its well-known geologic history, excellent microfossil preservation, and well known sequence stratigraphic framework due to the drilling efforts of ODP Legs 150X and 174AX. The foundation of this study (Chapter 1) includes a detailed sequence stratigraphic analysis of Paleocene-lowermost Eocene sequences. This study identified seven Paleocene-lowermost Eocene sequences and corresponding paleodepths. Paleodepths were ~80 m during the early Paleocene, with a maximum water depth of 100 m at Bass River. A long-term shallowing of ~20 m is observed through the middle-late Paleocene toward the earliest Eocene. The shallowing trend reverses, with a water depth increase to ~120-150 m (Chapter 2). The New Jersey sequences correlate with those in other localities and δ18O increases in deep sea (Chapter 3), suggesting a glacioeustatic mechanism for sea-level change during the Paleocene-earliest Eocene. The New Jersey coastal plain also provides an excellent record of the Paleocene- Eocene Thermal Maximum (PETM) and Carbon Isotope Excursion (CIE). The PETM/CIE is distinguished by the occurrence of an unusual assemblage of coccolithophorids, referred to as the "RD". This study tested the first appearance of the RD in relation to a CIE precursor found in the New Jersey coastal plain and utilized paleodepths to construct one of the few sea-level records calibrated to the PETM and the mechanisms responsible for a 30-60 m eustatic rise during this interval.Ph.D.Includes abstractVitaIncludes bibliographical referencesby Ashley D. Harri
18F-fluoroethylcholine (18F-cho) PET/MRI functional parameters in pediatric astrocytic brain tumors
No full textPurpose: to examine the feasibility of simultaneous acquisition of 18F-fluoroethylcholine (18F-choline) PET and functional MRI (standardized uptake value [SUV]max/mean and apparent diffusion coefficient [ADC]mean), using a hybrid PET/MRI scanner, for diagnosis and response assessment in a cohort of children with astrocytic brain tumors.Methods: 18F-choline PET/MRI scans were performed in 12 patients with proven astrocytic tumors. Eight patients simultaneously underwent 18F-choline PET/MR follow-up scans after treatment. Uptake in the lesion above the normal brain activity was considered indicative of a positive scan. Maximum and mean SUVs (SUVmax and SUVmean) and mean ADC (ADCmean) of the whole tumor region of interest were assessed. Lesion size and contrast enhancement were recorded. For all tumors, the association between ADCmean and SUVmean/ SUVmax values were assessed using the Spearman correlation coefficient.Results: at baseline, the areas of 18F-choline uptake matched areas of contrast enhancement and restricted diffusion. There was a negative correlation trend between SUVmax and ADCmean and a positive correlation trend between SUVmax and tumor size. There was concordance between reduction in tumor size and reductions in SUVmax and SUVmean in 4 children, in three of whom ADCmean values were increased. In 2 patients, tumor size remained stable whereas SUVmax and SUVmean values were increased with reduction in the ADCmean values. Additionally, in 2 children, cross-sectional MRI showed an increase both in tumor size and SUVmax but a reduction in ADC values.Conclusions: simultaneous 18F-choline PET/MRI is a promising and reliable imaging tool for children with astrocytic tumors, as it permits monitoring of morphological and metabolic response and changes during therapy
Writing Faith: Text. Sign. and History in the Miracles of Sainte Foy
No full textA trickster saint whose miracles reportedly included the healing of an inguinal hernia via a hammer and anvil, Sainte Foy inspired one of the most important collections of miracle stories of the central middle ages. Kathleen Ashley and Pamela Sheingorn explore the act of writing faith as performed both by the authors of these stories and by the scholars who have used them as sources for the study of medieval religion and society. As Ashley and Sheingorn show, differing agendas shaped the miracle stories over time. The first author, Bernard of Angers, used his narratives to critique popular religion and to establish his own literary reputation, while the monks who continued the collection tried to enhance their monastery\u27s prestige. Because these stories were rhetorical constructions, Ashley and Sheingorn argue, we cannot use them directly as sources of historical data. Instead, they demonstrate how analyzing representations common to groups of miracle stories—such as negative portrayals of Muslims on the eve of the Crusades—can reveal the traces of history.https://digitalcommons.usm.maine.edu/facbooks/1372/thumbnail.jp
Biological activities of Murdannia species / Ashley Wasli and Nor Aziyah Bakhari
No full textNumerous studies have recently revealed that the genus Murdannia is well-known for its biological activities that gives them the potential use as cure for certain diseases. Biological activities of the genus Murdannia is evaluated through the contributing factor of bioactive compound that exist in the genus itself. A review and comparison study of the related literature to the biological activities of Murdannia species has been conducted in this study. The papers were gathered using online database search (Science Direct, Springer, ResearchGate, Scopus, Wiley, Taylor & Francis and others) and classified by their publication year, author, published journal, parameters, and methods. In vitro study of M. Bracteata crude extracte revealed to have anti-oxidant, cytotoxic, hepatoprotective and α-glucosidase activity. Roots extract of M. Lanuginosa has been identified to posses anti-microbial, anti-inflammatory and anti-proliferative activity. M. Simplex roots extract revealed fungicidal activity due to the presence of protamine. Crude extract of M. Nudiflora demonstrated analgesic, anti-inflmmatory, anti-oxidant, and cytotoxic activity due to the presence of major phenolic compounds. M. Loriformis possess antiinflammatory, anti-oxidant antimutagenicity, anti-ulcerogenic analgesic, DT-diaphrose and antipyretic activity which influenced by chemical constituents such as alkaloids, amino acids, flavonoids, and polyphenols that exist in the plant extract
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Six New and Recent Works for Solo Cello in Recording and Discussion
No full textABSTRACT OF THE DISSERTATIONSix New and Recent Works for Solo Cello in Recording and Discussionby Ashley Melynda WaltersDoctor of Musical Arts in Contemporary Music PerformanceUniversity of California, San Diego, 2013Professor Charles Curtis, ChairThe focus of this dissertation project was on the learning, performance, and recording of six works for solo cello: Sequenza XIV by Luciano Berio, Plainsound-Litany by Wolfgang von Schweinitz, Another Secular Calvinist Creed by Andrew McIntosh, Sweet Bay Magnolia with Berry Clusters by Wadada Leo Smith, For Stephanie (on our wedding day) by Nicholas Deyoe, and another anxiety by Nicholas Deyoe. The following writing serves as a supporting document to this project, concentrating on the collaborations between the author and Schweinitz, McIntosh, Smith, and Deyoe. This paper will address the variations in the collaborative process with each composer, commenting on how the dedication of the piece to the author affected the inspiration for writing the piece and the notation, where relevant. In addition, the influence of the collaborative process on the author's interpretation of each piece will be discussed. Finally, where applicable, these aspects and their influence on the listening experience will be addressed
The treatment of abused black children through a structured group experience, 1986
No full textThe purpose of this study was to investigate the effects of a structured group experience on the psychosocial conditions of abused Black children. Significance of the Study The study suggests a method of treatment of abused Black children as it relates to their psychosocial condition. Although treatment services for different groups may be quite similar, preven�tion strategies and treatment must take into account the individual and his/her unique values, cultural norms and socialization patterns. Methods and Procedures The data were analyzed statistically. Analysis of Variance (ANOVA) was used to determine statistically if there was a significant difference between the mean of the control group and the experimental group. This was the criterion used to determine the impact of the structured group experience in ameliorating and facilitating positive improvement in the psychosocial condition of abused Black children. Instruments The instruments used in this study were the Self-Esteem Inven�tories for Children and Adults (CF/SEI), the Carlson Psychological Sur�vey (CPS), and the Psychological Screening Inventory (PSI). Conclusions The abused child has been repeatedly described as exhibiting inappropriate handling of aggression impulses, negative self-images, difficulties in relating to peers, in forming attachments and in making separations and inability to trust others. The structured group experience provided a teaching mechanism that can be used to bring about a positive behavioral change in its members. This is supported by the findings of this study. The ultimate goal of this structured group experience is to help the abused child to recognize his worth as a person and develop mechanisms of coping with the feelings resulting from abuse. The findings of this study demonstrate the positive effects of a structured group experience addressing the psychosocial condition of this clientele. An important outcome of this study was that the participants were instructed in appropriate interaction and exploration of their feelings. This structured group experience provided the appropriate psy�chosocial climate for the discussion of problems and the development of effective coping mechanism to be used by the abused child. More importantly, this structured group experience provides abused Black children with a mechanism for learning to be involved with, rather than isolated from, other children and can often be a crucial prerequisite to individual counseling
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