1,720,978 research outputs found
Recensione del volume "Architetture ENI in Italia" di Laura Greco, Stefania Mornati
No full textrecensione del volume "Architetture ENI in Italia" di Laura Greco, Stefania Moranati, Gangemi, 201
The effect of Phytochemical Compounds on Uterine Myometrial and Leiomyoma Cells
Full text linkIl fibroma uterino o leiomioma è il tumore benigno più comune che colpisce l’utero nelle donne in età fertile. I fibromi causano, dolore o pressione pelvica, menorragia ossia una perdita ematica mestruale abbondante nonché dolorosa, ed è una delle principali cause d’infertilità e aborti ricorrenti. I fibromi uterini, sono l’indicazione principale d’isterectomia. Nonostante, l'alta prevalenza di questa patologia nelle donne, la patogenesi dei leiomiomi uterini non è stata ancora del tutto chiarita. Recenti studi, hanno dimostrato che una dieta basata su composti fitochimici ha proprietà preventive su diverse malattie. Il fibroma uterino è caratterizzato da un’alta componente extracellulare (ECM), in particolare collagene, fibronectina e proteoglicani, e da fattori di crescita, da citochine e chemochine, da fattori angiogenici e da mediatori di risposta infiammatoria. Nel presente studio, abbiamo cercato di indagare sull’effetto dei composti fitochimici su cellule di fibroma e di miometrio in vitro, in particolare se fossero in grado di regolare l’espressione genica e proteica dei componenti della matrice extracellulare. Per il nostro studio abbiamo utilizzato 2 diverse cultivar di fragole (Alba e Romina) e la frazione di Romina arricchita di antociani o ACY, per valutare l’effetto antifibrotico sia sulle cellule di fibroma che su quelle di miometrio. Le cellule di fibroma e di miometrio sono state trattate con estratti delle diverse cultivar, utilizzando per entrambi 2 diverse concentrazioni (100 μg/ml e 250 μg/ml) e 250 μg/ml per ACY, per 48 ore, con lo scopo di valutare la variazione dell'espressione genica e proteica dei componenti associati alla fibrosi. Abbiamo ottenuto, che sia nelle cellule di leiomioma che in quelle di miometrio, l’espressione genica di collagene1A1, fibronectina, versican e activin-A è diminuita significativamente; inoltre, si ha una riduzione significativa dell’espressione proteica della fibronectina rispetto al controllo. Successivamente, abbiamo voluto testare, altri costituenti fitochimici tra cui la Quercetina e l’Indolo- 3-Carbinolo. La quercetina (3,3 ', 4', 5,7-pentahydroxyflavone) è un bioflavonoide vegetale, comune nella maggior parte dei frutti e vegetali, mentre l'indolo-3-carbinolo (I3C; 1H-indolo-3-ilmetanolo) è un derivato dei glucosinolati presente in numerose piante, compresi i membri della famiglia Cruciferae e in particolare i membri del genere Brassica. Le cellule di leiomioma e di miometrio sono state trattate, in vitro, con la quercetina e l’indolo-3- carbinolo a diverse concentrazioni (10 μg/ml, 50 μg/ml, 100 μg/ml, 250 μg/ml) per 48 ore, per misurare l’espressione genica e proteica delle componenti della matrice extracellulare. Si è visto, che sia la quercetina che l’indolo-3-carbinolo riducono significativamente l'espressione genica di collagene1A1 e della fibronectina nelle cellule di leiomioma, l'espressione proteica della fibronectina nelle cellule di leiomioma. Inoltre, grazie ai saggi di wound healing e di proliferazione, si è vista un’inibizione della migrazione e della proliferazione cellulare, delle cellule di leiomioma, in coltura successivamente al trattamento con la quercetina e l’indolo-3-carbinolo. In conclusione, questo studio mostra gli effetti in vitro di alcuni costituenti fitochimici e getta le basi scientifiche per lo sviluppo di nuovi agenti terapeutici e/o preventivi per i leiomiomi uterini.Uterine leiomyomas (fibroids or myomas) are the most common benign tumors of the female reproductive tract. Fibroids cause pelvic pain, abnormal vaginal bleeding, pressure on the bladder, miscarriage, and infertility. They are the leading indication for hysterectomy. Unfortunately, no long- term medical treatments are available. In modern pharmaceutical industries, dietary phytochemicals are used as a source of new potential drugs for many kinds of tumors. Dietary phytochemicals may exert therapeutic effects by interfering with key cellular events of the tumorigenesis. Since uterine leiomyoma is characterized by excessive accumulation of extracellular matrix (ECM) including collagens and fibronectin, in the present study, we aimed to investigate if dietary phytochemicals can regulate the ECM in human myometrium and leiomyoma cells. In the present study, initially, we aimed to test the antifibrotic effect of 2 different strawberry cultivars (Alba and Romina) and the anthocyanin enriched fraction(ACY) of Romina in uterine leiomyoma and myometrial cells. Leiomyoma and myometrial cells were treated with 2 different concentrations (100 μg/ml and 250 μg/ml) and with 250 μg/ml anthocyanin enriched fraction (ACY), for 48 h to measure gene expression and/or protein associated with fibrosis. We show, significantly decreased of collagen1A1, fibronectin, versican, and activin-A mRNA expression, in leiomyoma cells as well as in myometrial cells. The reduced protein expression of fibronectin was also observed after treatments with those cultivars in leiomyoma cells compared with untreated control. Afterward, I has been involved in testing other phytochemical compounds on leiomyoma cells such as Quercetin and Indole-3-Carbinol. Quercetin (3,3′,4′,5,7-pentahydroxyflavone) is a plant bioflavonoid, found in most edible fruits and vegetables. Indole-3-Carbinol (I3C; 1H-indole-3-ylmethanol) is produced from naturally occurring glucosinolates contained in a wide variety of plants, including members of the family Cruciferae and particularly members of the genus Brassica. Leiomyoma and myometrial cells were treated with Quercetin and I3C (10 μg/ml; 50 μg/ml; 100 μg/ml; 250 μg/ml) for 48 h to measure mRNA and protein expressions of ECM, as well as to evaluate the migration (by wound healing assay) and the proliferation rate. Quercetin and I3C significantly decreased collagen1A1 and fibronectin mRNAs expression and protein expression of fibronectin in leiomyoma cells. We also, obtained a significant reduction of the migration and proliferation in leiomyoma and myometrium cells, after treatment with quercetin and I3C. In conclusion, this study shows the in vitro effects of some phytochemical compounds and lays the scientific bases for the development of new therapeutic and/or preventive agents for the uterine leiomyomas
Impact of Nutrition on Female Reproductive Disorders
No full text: The female reproductive system is a delicate and complex system in the body that can be affected by many disorders [...]
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
- …
