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    Metabolic profile of the bioactive compounds of burdock (Arctium lappa) seeds, roots and leaves

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    In this work the bioactive metabolic profile, the antioxidant activity and total phenolic content of burdock (Arctium lappa) seeds, leaves and roots were obtained. TEAC values and total phenolic content for hydro-alcoholic extracts of burdock ranged from 67.39 to 1.63 μmol Trolox equivalent/100 g dry weight (DW), and from 2.87 to 45 g of gallic acid equivalent/100 g DW, respectively. Phytochemical compounds were analyzed by liquid chromatography coupled to electrospray tandem mass spectrometry (LC/MS/MS) in negative mode. The main compounds of burdock extracts were caffeoylquinic acid derivatives, lignans (mainly arctiin) and various flavonoids. The occurrence of some phenolic acids (caffeic acid, chlorogenic acid and cynarin) in burdock seeds; arctiin, luteolin and quercetin rhamnoside in burdock roots; phenolic acids, quercetin, quercitrin and luteolin in burdock leaves was reported for the first time

    Giovani, nuovi media e percorsi di orientamento nello spazio pubblico

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    This article presents the results of a survey that set out to reconstruct the relationship between young people, media usage and public space. Care was taken to move beyond the limits inherent in the concept of political participation, which does not necessarily offer the most effective angle for analysing the ways in which young citizens perceive and experience this relationship, which may not be one of engagement , in the arena of public discourse. We used in-depth interviews to identify the strategies young people adopt within multiple communication environments: family and the home, traditional and digital media, paying particular attention to social media. We classified the ways in which they access and process information, based on different levels of selectivity and different kinds of activity in the digital environment. We investigated some of the dynamics of opinion leadership, both interpersonal and media-related, and observed various interesting phenomena regarding the fusion of the diverse discourses that exercise an influence on a cross-media level, and that become orientation agents in public space. This takes place in the family, the education experience and friendship networks. Another significant factor was the emergence of public figures whose discourses cross and connect different media universes, from television to social media (such as Pope Francis, the leader of the Northern League Party Matteo Salvini, the writer Roberto Saviano and the singer Madonna). The issue of migration stands out as the most recent, most significant dividing line in youth civic culture

    Microwave-assisted exctraction of Ochratoxin A from roasted coffee: an alternative extraction approach.

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    Microwave-assisted extraction (MAE) followed by high performance liquid chromatography (HPLC) with a fluorescent detector (DAD) was used and developed for the quantitative analysis of the mycotoxin ochratoxin A (OTA) in commercial roasted coffee beans. This alternative approach has been compared with the conventional extraction that uses hydrogen carbonate aqueous solution followed by OchraTest immunoaffinity analysis. The effect of two experimental tunable MAE parameters (temperature and pressure) on the extraction efficiency of OTA have been investigated using coffee samples fortified at different contamination levels. The optimum extraction conditions were obtained using a temperature of 50 °C and a 500 W microwave power. OTA quantity extracted using MAE was similar to that obtained by conventional extraction from samples fortified at 5, 10, and 100 ng g−1 levels. At a 20 ng g−1 level, MAE was more effective than the conventional method. The MAE setting parameters have been optimized showing both extraction time and solvent consumption have been considerably reduced, retaining high OTA recovery values

    Microwave Assisted extraction of Phenolic Compounds of Four Different Spices

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    Spices and herbs are known not only for their taste, aroma and flavour, but also for their medical properties and value. Both spices and herbs have been used for centuries in traditional medical systems to cure various kinds of illnesses such as common cold, diabetes, cough and cancers. The aim of this work was the comparison between two different extractive techniques in order to get qualitative and quantitative data regarding bioactive compounds of four different spices (Cinnamomum zeylanicum, Coriandrum sativum, Cuminum cyminum, Crocus sativus). The plants were extracted employing ultrasonication and microwave-assisted extractions. The efficiency of extraction of bioactive compounds obtained with the microwave extraction process was in general about four times higher than that resulting from sonication extraction. The various extracts obtained were analyzed for their antioxidant activity using ABTS, DPPH and FRAP assays and for their total polyphenolic content. It can be concluded that microwave-assisted extractions provide significant advantages in terms of extraction efficiency and time savings
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