1,721,286 research outputs found
Saxton, Bob interview
No full textOral History interview of Bob Saxton. Interview conducted by Gray, Matthew at Veteran\u27s Residence
Hickey, Margaret A. interview
No full textOral History interview of Margaret Hickey. Interview conducted by Gray, Matthew at Orlando, FL
Cytochrome C : its role in methylotrophic bacteria
No full textThis thesis investigates the role of cytochrome c in methylotrophic bacteria. The majority of the experiments have been performed using two organisms, the facultative methylotroph Pseudomonae AM and the obligate methylotroph Methylophilus, methylotrophue. Neither the concentration of membrane-bound cytochrome c nor the HtJO ratio measured in Pseudomonas AM vary with varying conditions of nutrient-limited growth. The methanol dehydrogenase from Methylophilus methylotrophus has been purified. It is a basic protein with a molecular weight of 120000, consisting of two identical sub units. It requires added methanol for stabilisation. Cyanide has been found to be a linear, competitive inhibitor for the substrate methanol and was able to replace methanol as the stabilising agent. It has been shown that the two major soluble cytochromes c (cytoehromes cH and CL) of Pseudomonas AMI and Methylophilus methylotrophus are completely distinct entities and that the two cytochromes cg are similar, as are the two cytochromes cL. Two distinct soluble cytochromes c have also been found in the facultative methylotrophs hyphomicrobium X and Paracoccus denitrificans. Both the pure, soluble cytochromes c from Methylophilus methylotrophus become reduced at alkaline pH in the absence of added reductant. The first-order kinetics of this process suggest that the reduction is intramolecular and thus one of autoreduction. A mechanism has been proposed to explain this observation. It has been shown that the pure methanol dehydrogenase, in the absence of added methanol, will reduce cytochrome cL, but not cytochrome cH, and it is proposed that methanol dehydrogenase lowers the pH for autoreduction by effecting a conformational change in cytochrome c. It has, however, been possible to demonstrate the methanol-dependent reduction of cytochrome c by methanol dehydrogenase, with concomitant formaldehyde formation, by coupling the reaction to the reduction of exogenous mammalian cytochrome c. By studying a similar reaction in Pseudomonas AMI and Paracoccus denitrificans it has been established that methanol dehydrogenase can only reduce one of the two soluble cytochromes c found in methylotrophs. The methanol dehydrogenase from a particular methylotroph usually only reduces cytochrome c from the same organism. The most important contribution of this thesis is the demonstration that the primary acceptor for electrons derived from the oxidation of methanol by methanol dehydrogenase is one of the two soluble cytochromes c found in methylotrophs.</p
Sustaining the ecological, social and economic values of the forests of Southern Tasmania
No full textThis thesis articulates a methodology that can be applied to the analysis and design of underlying organisational structures and processes that will consistently and effectively address ‘wicked problems’ (the most difficult class of problems that we can conceptualise: problems which consist of ‘clusters’ of problems; problems within these clusters cannot be solved in isolation from one another, and include sociopolitical and moral-spiritual issues (Rittel and Webber 1973)) in forestry. This transdisciplinary methodology has been developed from the perspective of institutional economics synthesised with perspectives from ecological economics and system dynamics.\ud
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The institutionalist policymaking framework provides an approach for the explicit development of holistic policy. \ud
An illustrative application of this framework has been\ud
applied to the wicked problem of forestry in southern Tasmania as an example of the applicability of the approach in the Australian context. To date all attempts to seek\ud
solutions to that prevailing wicked problem set have relied on non-reflexive, partial and highly reductionist thinking.\ud
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A formal assessment of prevailing governance and process arrangements applying to that particular forestry industry has been undertaken using the social fabric matrix.\ud
This methodology lies at the heart of the institutionalist policymaking framework, and allows for the systematic exploration of elaborately complex causal links and\ud
relationships, such as are present in southern Tasmania.\ud
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Some possible attributes of an alternative approach to forest management that sustains ecological, social and economic values of forests have been articulated as\ud
indicative of the alternative policy and management outcomes that real-world application of this transdisciplinary, discursive and reflexive framework may\ud
crystallise. Substantive and lasting solutions to wicked problems need to be formed endogenously, that is, from within the system. The institutionalist policymaking\ud
framework is a vehicle through which this endogenous creation of solutions to wicked problems may be realised
Design and analysis of ranavirus studies: surveillance and assessing risk
Full text linkRanaviruses are pathogens that cause disease in ectothermic vertebrate species, and are responsible for die-off events in multiple taxa across the globe. Understanding the threat of ranavirus in wild and captive populations is a growing conservation and economic interest. Quantifying risk is a central premise to understanding the threat of a pathogen, and surveillance studies are a logical starting point. In this chapter, we discuss how to design surveillance studies for ranavirus, required sample sizes, statistical analyses commonly used to analyze data, and modeling approaches to predict disease outcomes. Additionally, we cover the process of Import Risk Analysis, which quantifies the threat of ranavirus introduction into a new area. The principles discussed herein will help organizations and investigators document the distribution of ranaviruses, identify hotspots of infection and disease, determine factors associated with emergence, and collect data necessary to determine practical intervention strategies
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Design and analysis of Ranavirus studies: Insights into planning surveillance, modeling host-pathogen dynamics, and performing risk analyses
No full textThis chapter is divided into three major sections: surveillance (Sect. 1), modeling (Sect. 2), and risk analyses (Sect. 3). Section 1 is designed to provide practical guidance on how to set up, collect, analyze, and interpret basic data that can be collected during pathogen surveillance in field or captive settings. We also discuss the economics of surveillance. Section 2 introduces typical models used to model pathogen transmission and methods used to estimate invasion potential, R0. Section 3 focuses on strategies that can be used to estimate the risk of ranavirus invading into a novel area. While detail is provided in these sections, our intent is not to discuss all surveillance, modeling, or risk analysis scenarios. If readers need additional guidance, we welcome you to reach out to the chapter authors or authors of the papers and books we cite
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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