67 research outputs found
Transformation of the 'Weg en Werken' area in Haarlem
No full textGraduation project A transformation of a former railway area in Haarlem into a Museumpark. A transformation of the Seinwezen building which is the main building in the plot. Two building have been added to the Seinwezen based on the essence, which is the shape. Interior walls have been removed to open it up. Through the three buildings runs a railway track.RMITArchitectur
Design, analysis, and control of a low power joint for walking robots, by phasic activation of McKibben muscles
No full textTwo-legged body
No full textThe invention relates to a two-legged body comprising at least two legs, an oscillation element and a connecting element for the mutual coupling of the legs and the oscillation element, wherein the legs are pivotable in relation to the connecting element and during operation undergo a reciprocating angular displacement in relation to the connecting element, depending on the oscillation element. The oscillation element comprises at least two springs for the generation of antagonistic forces, which springs each are coupled at one side with a leg, and together with the mass present in the body they are able to form a vibrating mass-spring system. The spring stiffness of at least one spring is adjustable.Mechanical, Maritime and Materials Engineerin
Bacteria from seawater used in Penaeus monodon larval cultures
No full textBacteria in the seawater used in P. monodon hatchery operations were isolated on Bachmann's agar. The total plate counts in 25 isolations ranged from 1.0 - 5.0 x 102 to 5.1 -10.0 x 105 cells per ml. Out of 124 isolates, 98 (79 percent) were Gram-positive and 26 (21 percent) were Gram-negative. Micrococcus and Staphylococcus were dominant in the former group, while Acinetobacter, Moraxella, Flavobacterium and Alcaligenes were most numerous in the latter. Twenty-nine of the Gram-positive isolates closely resembled Peptostreptococcus, Planococcus, and Pediococcus
Acidiferrimicrobium australe gen. nov., sp. nov., an acidophilic and obligately heterotrophic, member of the actinobacteria that catalyses dissimilatory oxido-reduction of iron isolated from metal-rich acidic water in Chile
No full textFunding Information: IN: This work was supported by Fondecyt 11150170. MAD was supported by grant DIUV-CIDI 4/2016 from Universidad de Valparaíso. RQ: This work was supported by the Comisión Nacional de Investigación Cientí-fica y Tecnológica (under Grants FONDECYT 1181251 to R.Q., Programa de Apoyo a Centros con Financiamiento Basal AFB170004 to R.Q.) and by Millennium Science Initiative, Ministry of Economy, Development and Tourism of Chile (under Grant ‘Millennium Nucleus in the Biology of the Intestinal Microbiota’ to R.Q.). Publisher Copyright: © 2020 The Authors.A novel acidophilic member of the phylum Actinobacteria was isolated from an acidic, metal-contaminated stream draining from an abandoned underground coal mine (Trongol mine), situated close to Curanilahue, Biobío Region, Chile. The isolate (USS-CCA1T) was demonstrated to be a heterotroph that catalysed under aerobic conditions the oxidation of ferrous iron and the reduction of ferric iron under anaerobic conditions, but not the oxidation of sulfur nor hydrogen. USS-CCA1T is a Gram-positive, motile, short rod-shaped, mesophilic bacterium with a temperature growth optimum at 30 °C (range 20–39 °C). It was catego-rized as an extreme acidophile growing between 1.7 and 4.5 and optimally at pH 3.0. The G+C content of the chromosomal DNA of the isolate was 74.1 mol%, which is highly related to Aciditerrimonas ferrireducens IC-180T, (the most closely related genus; 94.4% 16S rRNA gene identity), and higher than other acidophilic actinobacteria. The isolate (USS-CCA1T) was shown to form a distinct 16S rRNA clade from characterized acidophilic actinobacteria, well separated from the genera Acidimicrobium, Ferrimi-crobium, Ferrithrix, ‘Acidithrix’ and Aciditerrimonas. Genomic indexes (ANIb, DDH, AAI, POCP) derived from the USS-CCA1T draft genome sequence (deposited at DDBJ/ENA/GenBank under the accession WJHE00000000) support assignment of the isolate to a new species and a new genus within the Acidimicrobiaceae family. Isolate USS-CCA1T is the designated type strain of the novel species Acidiferrimicrobium australe (=DSM 106828T,=RGM 2506T)
Hilo harbor tsunami model - reflected waves superimposed
No full textThe city and harbor of Hilo, located on the northeast coast of the island of Hawaii, have been severely damaged by numerous tsunamis. Physical features which play an important role in the formation of tsunami bores at Hilo are the submarine ridge formations in deep water outside the bay mouth and the nearly vertical cliffs along the Hamalcua coast which reflect the tsunami wave into the harbor. The purpose of this paper is to discuss the Hilo Harbor Tsunami Model and this reflected wave and its effects. The tests described and the resultant information presented herein, unless otherwise noted, were obtained from research conducted under the Hilo Harbor Model Study of the United States Army Corps of Engineers by the Honolulu Engineer District. The permission granted hy the Chief of Engineers to publish this information is appreciated
Genomic evolution of the class Acidithiobacillia : deep-branching Proteobacteria living in extreme acidic conditions
No full textFunding Information: Acknowledgements This work was supported the Agencia Nacional de Investigación y Desarrollo under Grants FONDECYT 1181251 (R.Q.), Programa de Apoyo a Centros con Financiamiento Basal AFB170004 (R.Q.), CONICYT-PFCHA/Doctorado Nacional/ 20171049 (A.M.B.), CONICYT-PFCHA/Doctorado Nacional/ 21160871 (F.I.), the ANID—Millennium Science Initiative Program— NCN17_093, granted by the Ministry of Economy, Development and Tourism from Chile (R.Q.), and Fundación Ciencia and Vida Hinge PostDoc Program (S.B.). Mónica Gonzales, Paulo Covarrubias and Hector Carrasco provided assistance with microbiological and molecular biology analyses at early stages of this study. Douglas Rawlings, Violaine Bonnefoy, Patricia Chiacharini, Jiri Kucera, Martin Mandl, Francisco Remonsellez, Pablo Ramirez Roca, and Mario Vera shared strains used in this study. We are grateful to Professor Aharon Oren (Hebrew University of Jerusalem Israel) for his help and advice on the projected names of novel bacterial genera and species. The authors thank the authorities of the Provincial Thermal Baths Agency (EPROTEN) and the Directorate of Protected Natural Areas (ANP) of the province of Neuquén Argentina for allowing access and sampling in the Copahue-Caviahue Provincial Park and Ing. Rubén Vargas (alias Caniche) for guidance during ascent to the Copahue volcano. Publisher Copyright: © 2021, The Author(s).Members of the genus Acidithiobacillus, now ranked within the class Acidithiobacillia, are model bacteria for the study of chemolithotrophic energy conversion under extreme conditions. Knowledge of the genomic and taxonomic diversity of Acidithiobacillia is still limited. Here, we present a systematic analysis of nearly 100 genomes from the class sampled from a wide range of habitats. Some of these genomes are new and others have been reclassified on the basis of advanced genomic analysis, thus defining 19 Acidithiobacillia lineages ranking at different taxonomic levels. This work provides the most comprehensive classification and pangenomic analysis of this deep-branching class of Proteobacteria to date. The phylogenomic framework obtained illuminates not only the evolutionary past of this lineage, but also the molecular evolution of relevant aerobic respiratory proteins, namely the cytochrome bo3 ubiquinol oxidases
Acidithiobacillus ferrianus sp. nov. : an ancestral extremely acidophilic and facultatively anaerobic chemolithoautotroph
No full textFunding Information: This work was supported in part by BHP Billiton Chile Inc. (PN), the Natural Environment Research Council, UK (CF and DBJ, Grant ref. NE/L014076/1) and the Comisión Nacional de Investigación Científica y Tecnológica (under Grants FONDECYT 1181251 (R.Q.), the Programa de Apoyo a Centros con Financiamiento Basal AFB170004 (R.Q.), CONICYT-PFCHA/Doctorado Nacional/20171049 (A.M.B.), CONICYT, PAI/Convocatoria Nacional Subvencion a la Instalación en la Academia Convocatoria 2019, PAI77190083 (M.C.), and the Millennium Science Initiative, Ministry of Economy, Development and Tourism of Chile (under Grant “Millennium Nucleus in the Biology of the Intestinal Microbiota”; A.M.B., M.C. and R.Q.). Acknowledgements Funding Information: Technical assistance of Sara Baker (University of Warwick), Barry M. Grail (Bangor University) and Yasna Gallardo (Fundación Ciencia y Vida) is gratefully acknowledged. Publisher Copyright: © 2020, The Author(s).Strain MG, isolated from an acidic pond sediment on the island of Milos (Greece), is proposed as a novel species of ferrous iron- and sulfur-oxidizing Acidithiobacillus. Currently, four of the eight validated species of this genus oxidize ferrous iron, and strain MG shares many key characteristics with these four, including the capacities for catalyzing the oxidative dissolution of pyrite and for anaerobic growth via ferric iron respiration. Strain MG also grows aerobically on hydrogen and anaerobically on hydrogen coupled to ferric iron reduction. While the 16S rRNA genes of the iron-oxidizing Acidi-thiobacillus species (and strain MG) are located in a distinct phylogenetic clade and are closely related (98–99% 16S rRNA gene identity), genomic relatedness indexes (ANI/dDDH) revealed strong genomic divergence between strain MG and all sequenced type strains of the taxon, and placed MG as the first cultured representative of an ancestral phylotype of iron oxidizing acidithiobacilli. Strain MG is proposed as a novel species, Acidithiobacillus ferrianus sp. nov. The type strain is MGT (= DSM 107098T = JCM 33084T). Similar strains have been found as isolates or indicated by cloned 16S rRNA genes from several mineral sulfide mine sites
Analyzing the Combined Role of Good Governance and GDP on Women Literacy in African Countries
No full textMost African countries have been affected due to economic crises regarding hyperinflation and lower agricultural productivity, civil war, lower infrastructural and transportation systems, social and domestic violence, food insecurity due to bad agricultural systems, social and religious inequality, mismanagement of governing systems with defective bureaucracy, environmental degradation and lower practice for sustainable development. In this paper, the author tries to find out two research objectives i) To measure the good governing variables on women literacy between East and West African countries ii) To measure the joint impact of good governance and GDP level between these two countries. The author uses secondary dataset from The World Bank site on quantitative analysis where the author uses multiple regression model for general regression and integrated regression model. At the time of multiple regression (Model 1), GDP, IF, P.S., R.Q. has a positive connection with women literacy for EAC. On the contrary, PHR, FLF, DOFC, R.L. create a negative effect on women's literacy in EAC. But in (Model 2), if the good governance variable connects with GDP for long-lasting development, (GDP*PS), (GDP*RQ) has positive connection with W.L. in EAC. Reversely, L.E., PHR, G.E., R.Q., CC has positive and significant impact in WAC where these variables enhance women literacy to be improved at significant rate (Model 3) where DOFC and EFC has reverse connection with W.L. In spite of case, if GDP connects with good governance variables, (GDP*RQ) and (GDP*PS) has positive connectivi-ty with W.L. in WAC. Policy makers should highlight to promote effective R.L. and G.E. to improve macroeconomic development in EAC where governments of WAC should focus on R.L. and G.E. to ensure women empowerment and social security for sustainable development. Establishing good governance's variables in African countries is carrying need to ensure women empowerment, lessening gender inequality, ensuring women safeness and sustainable development etc
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