1,721,034 research outputs found
Airway involvement in pulmonary tuberculosis
Get PDFThe original publication is available at http://www.samj.org.zaPlease cite as follows: Goussard, P. & Gie, R. 2007. Airway involvement in pulmonary tuberculosis. South African Medical Journal, 97(10):986-988.[No abstract available]Publisher’s versio
The elimination of fanleaf virus from grapevines using in vitro somatic embryogenesis combined with heat therapy
Get PDFCITATION: Goussard, P. G. & Wiid, J. The elimination of fanleaf virus from grapevines using in vitro somatic embryogenesis combined with heat therapy. South African Journal of Enology & Viticulture, 13(2):81-83, doi:10.21548/13-2-2180.The original publication is available at http://www.journals.ac.za/index.php/sajevSomatic embryos were successfully regenerated from callus tissue of anthers and ovaries excised from inflorescences of grapevines infected with grapevine fanleaf virus (GFLV). Production of pro-embryogenic masses (PEMS) was controlled by specific growth regulators and culture conditions, including heat incubation at 35°C. Somatic embryos (containing roots and cotyledons) and plantlets were subjected to immunosorbent electron microscopy (ISEM) and serological tests (ELISA). Results show that somatic embryogenesis in combination with heat therapy of the cultures is an effective procedure to eliminate GFLV from anther and ovary source material.http://www.journals.ac.za/index.php/sajev/article/view/2180Publisher's versio
Anatomical responses to cytokinins of abscised grapevine shoot apices cultured in vitro
Get PDFCITATION: Goussard, P. G. 1987. Anatomical responses to cytokinins of abscised grapevine shoot apices cultured in vitro. South African Journal of Enology & Viticulture, 8(2):53-59, doi:10.21548/8-2-2315.The original publication is available at http://www.journals.ac.za/index.php/sajevResponses of excised shoot apices of V. vinifera L. cv. Chenin blanc to different cytokinin treatments were studied at various intervals (days) after the start of in vitro culture, using light and scanning electron microscopy. Results clearly indicated that in vitro produced shoots were of axillary origin. Shoot clusters were produced by the enhanced release of axillary meristems from apical dominance, due to the application of 6-benzylaminopurine (BAP) singly as well as in combination with zeatin riboside (ZR). Axillary meristems on these axillary shoots were subsequently released from apical dominance, thus giving rise to shoot clusters of high densities. Shoot clusters induced by ZR alone were less dense, probably due to an initial delay in elongation of axillary meristems nearest to the main apical meristem of the shoot. However, elongation of axillary meristems lower down the axes proceeded strongly in the presence of ZR. Application of BAP resulted in more pronounced release of axillary meristems from apical dominance than with ZR.http://www.journals.ac.za/index.php/sajev/article/view/2315Publisher's versio
In vitro culture of ovules and embryos from seedless grapes (vitis vinifera L.)
Get PDFCITATION: Burger, P. & Goussard, P. G. 1996. In vitro culture of ovules and embryos from seedless grapes (vitis vinifera L.). South African Journal of Enology & Viticulture, 17(2):31-37, doi:10.21548/17-2-2255.The original publication is available at http://www.journals.ac.za/index.php/sajevOvules of stenospermocarpic seedless grapes were cultured under different conditions. The number of embryos was not significantly increased when ovules of Muscat Seedless were cultured on a medium supplemented with the plant growth regulators indole-3-acetic acid and gibberellic acid compared to the basal medium. No correlation was found between the ovule size of Sultanina and the presence of embryos. Comparable percentages of embryos were found in small, medium and large ovules following weekly culture from four to 10 weeks after bloom. Varying results were obtained when ovules of four open-pollinated seedless cultivars were cultured on two basal media with or without activated charcoal. In all four cultivars the highest number of embryos was found at the later culture date. With three cultivars the best results were obtained when ovules were cultured on the medium of Bouquet & Davis (1989), supplemented with activated charcoal. Isolated embryos of Muscat Seedless and Sunred Seedless were cultured under various conditions and it was found that the absence of growth regulators resulted in the highest number of plantlets with normal leaves.http://www.journals.ac.za/index.php/sajev/article/view/2255Publisher's versio
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
The ontogeny of somatic embryos from in vitro cultured grapevine anthers
Get PDFCITATION: Newton, D. J. & Goussard, P. G.. 1990. The ontogeny of somatic embryos from in vitro cultured grapevine anthers. South African Journal of Enology & Viticulture, 11(2):70-75, doi:10.21548/11-2-2271.The original publication is available at http://www.journals.ac.za/index.php/sajevTranslucent light green to yellow anthers excised from Vitis rupestris cv. Rupestris du Lot flower buds, produced from cuttings grown in a climate-controlled room, produced somatic embryos when cultured on modified Nitsch & Nitsch (1969) medium supplemented with l μM 6-benzylaminopurine (BAP) and 5 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Anatomical features of developing embryogenic explants were studied at various intervals, before and after the start of in vitro culture, using light microscopy. Results indicated that callus was entirely of somatic origin. Embryogenic cells were apparently present before in vitro culture commenced and multiplied sufficiently within the first 15 days' culture to form visible embryogenic growth centres. Embryogenic callus formed from the lateral and abaxial walls of the anther, all connective tissues and the filament. Somatic embryos were observed after 60 days of culture and possessed primordial vascular tissues and secondary embryoids. No vascular connections between somatic embryos were observed.http://www.journals.ac.za/index.php/sajev/article/view/2271Publisher's versio
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
The use of pediatric flexible bronchoscopy in the COVID-19 pandemic era
Get PDFOn March 11, 2020, the World Health Organization (WHO) declared the pandemic because of a novel coronavirus, called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In January 2020, the first transmission to healthcare workers (HCWs) was described. SARS-CoV-2 is transmitted between people because of contact, droplets, and airborne. Airborne transmission is caused by aerosols that remain infectious when suspended in air over long distances and time. In the clinical setting, airborne transmission may occur during aerosol generating procedures like flexible bronchoscopy. To date, although the role of children in the transmission of SARS-CoV-2 is not clear the execution of bronchoscopy is associated with a considerably increased risk of SARS-CoV-2 transmission to HCWs. The aim of this overview is to summarize available recommendations and to apply them to pediatric bronchoscopy. We performed systematic literature searches using the MEDLINE (accessed via PubMed) and Scopus databases. We reviewed major recommendations and position statements published at the moment by the American Association for Bronchology and Interventional Pulmonology, WHO, European Center for Disease Prevention and Control and expert groups on the management of patients with COVID-19 to limit transmission among HCWs. To date there is a lack of recommendations for safe bronchoscopy during the pandemic period. The main indications concern adults and little has been said about children. We have summarized available recommendations and we have applied them to pediatric bronchoscopy
Pediatric bronchoscopy: recent advances and clinical challenges
No full textIntroduction: During the last 40 years equipment has been improved with smaller instruments and sufficient size working channels. This has ensured that bronchoscopy offers therapeutic and interventional options.Areas covered: We provide a review of recent advances and clinical challenges in pediatric bronchoscopy. This includes single-use bronchoscopes, endobronchial ultrasound, and cryoprobe. Bronchoscopy in persistent preschool wheezing and asthma is included. The indications for interventional bronchoscopy have amplified and included balloon dilatation, endoscopic intubation, the use of airway stents, whole lung lavage, closing of fistulas and air leak, as well as an update on removal of foreign bodies. Others include the use of laser and microdebrider in airway surgery. Experience with bronchoscope during the COVID-19 pandemic has been included in this review. PubMed was searched for articles on pediatric bronchoscopy, including rigid bronchoscopy as well as interventional bronchoscopy with a focus on reviewing literature in the past 5 years.Expert opinion: As the proficiency of pediatric interventional pulmonologists continues to grow more interventions are being performed. There is a scarcity of published evidence in this field. Courses for pediatric interventional bronchoscopy need to be developed. The COVID-19 experience resulted in safer bronchoscopy practice for all involved
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