1,721,164 research outputs found
NanoString Profiling of Canine Glioma Treated with Neoadjuvant RNA Lipid Particle Aggregates
No full textTumor tissues were harvested from canines that had been treated with neoadjuvant RNA Lipid Particle Aggregates (RNA-LPA, n = 3) as well as from untreated controls (n = 3). The collected tissues were immediately collected in RNAlater. The following day, the preserved tissues underwent RNA extraction and were subsequently processed using Nanostring nCounter Canine Immuno Oncology Panel (cat. 115000465). This panel facilitated the quantification of gene expression within key immune response and cancer-related pathways
Impact of RNA Lipid Particle Aggregates on the Tumor Microenvironment in a Murine Brain Tumor Model
No full textMice bearing KR158b-luc intracranial tumors underwent treatment with one of the following: lipid particles, silenced RNA-loaded lipid particle aggregates (modRNA-LPA), or unsilenced RNA-loaded lipid particle aggregates. Twenty-four hours post-treatment, the tumor tissues were preserved in RNAlater, followed by RNA extraction. The samples were then dispatched to Novogene for library preparation via Poly(A) capture. Sequencing was conducted on the Illumina NovaSeq 6000 platform, utilizing a paired-end 150 bp sequencing approach. The RNA used for treatment was synthesized from total RNA extracted from the parental KR158b-luc cell line. A cDNA library was generated using RT-PCR prior to its use in in vitro transcription (IVT) reactions, followed by RNA cleanup to obtain purified mRNA antigens. The unsilenced RNA was produced using the ThermoFisher Scientific IVT kit (Cat. AM1344), while the silenced RNA was generated with the CellScript IVT kit (Cat. C-ICTAMY110510)
Analyzing the Impact of RNA Lipid Aggregates on Fibroblast Reticular Cells via RNA Sequencing In Vitro
No full textFibroblastic reticular cells (FRCs) were cultured in 6-well plates and treated with lipid particles (NP samples, n = 3 wells) or RNA-loaded lipid particle aggregates (RNA samples, n = 3 wells). 24 hours later, the cells were harvested, and RNA immediately extracted. The RNA samples were then sent to Novogene for library preparation. Sequencing was conducted on the Illumina NovaSeq 6000 platform, utilizing a paired-end 150 bp sequencing approach. The RNA used for treatment was GFP mRNA, obtained from Trilink (cat. L-7601)
Recomendaciones al momento de recepción de los pollitos
Full text linkLa recepción de los pollitos bebés es un momento crítico para la vida del animal. En este período el ave es más vulnerables a los cambios de temperatura, falta de agua, alimentación insuficiente, enfermedades causadas por condiciones sanitarias ineficientes. Recomendaciones para disminuir la mortalidad de los pollitos.EEA Santa CruzFil: Morales, Cristian Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santa Cruz; Argentina.Fil: Haro Gomez, Hector Mario. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santa Cruz; Argentina
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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