3,316 research outputs found
Dimer-dimer stacking interactions are important for nucleic acid binding by the archaeal chromatin protein Alba
No full textArchaea use a variety of small basic proteins to package their DNA. One of the most widespread and highly conserved is the Alba (Sso10b) protein. Alba interacts with both DNA and RNA in vitro, and we show in the present study that it binds more tightly to dsDNA (double-stranded DNA) than to either ssDNA (single-stranded DNA) or RNA. The Alba protein is dimeric in solution, and forms distinct ordered complexes with DNA that have been visualized by electron microscopy studies; these studies suggest that, on binding dsDNA, the protein forms extended helical protein fibres. An end-to-end association of consecutive Alba dimers is suggested by the presence of a dimer-dimer interface in crystal structures of Alba from several species, and by the strong conservation of the interface residues, centred on Are and Phe(60). In the present study we map perturbation of the polypeptide backbone of Alba upon binding to DNA and RNA by NMR, and demonstrate the central role of Phe(60) in forming the dimer dimer interface. Site-directed spin labelling and pulsed ESR are used to confirm that an end-to-end, dimer dimer interaction forms in the presence of dsDNA.Peer reviewe
First person – Alba Delrio-Lorenzo
No full textFirst Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Alba Delrio-Lorenzo is first author on ‘Sarcoplasmic reticulum Ca2+ decreases with age and correlates with the decline in muscle function in Drosophila’, published in JCS. Alba is a PhD student in the lab of Javier García-Sancho and María Teresa Alonso at the Instituto de Biología y Genética Molecular (IBGM), University of Valladolid, Spain, investigating the molecular mechanisms implicated in aging, particularly muscle aging.Peer reviewe
The Narrative and Dialogical Approaches to Social Representations Theory
No full textIntroduction: Current article presents a study conducted within a project of meta-theoretical analysis on the part of body of Social Representations literature launched by Annamaria Silvana de Rosa in 1994 (de Rosa 1994, 2001a, 2001b, 2002, 2013, 2016) with the goal of assessing the influence of the scientific production driven by narrative approach in Social Representations Theory. It is a part of EC-funded project (ITN-People MSCA-IDP 2013, no. 607279 http://www.europhd.eu/SoReComJointIDP) led by de Rosa at the European/International Joint PhD on Social Representations and Communication Research Centre and Multimedia Lab.
Aim: The aim is to take stock of the scientific field developed in more than 50 years by conducting an empirical meta-theoretical analysis of the literature on Social Representations, mapping the development of dialogical and narrative paradigmatic approaches and linked to them rhetorical and discursive/conversational research traditions, their research methods, the thematic areas and their impact on the various applied fields within the multi-generational community of scientists and across different geo-cultural contexts.
Data sources and Methodology: Bibliographic sources using dialogical and narrative paradigmatic approaches as well as sources referring to the rhetorical and discursive/conversational research traditions in Social Representations Theory were extracted from the SoReCom “A.S. de Rosa”@-Library bibliographic repository (de Rosa, 2014, 2015) and analysed using the Grid for meta-theoretical analysis developed by de Rosa in 1994 (updated version 2014d). Statistical analyses have supported the identification of the key results under the lens of Multiple Correspondence Analysis, the Descending Hierarchical Cluster Analysis and The Correspondence Factorial Analysis.
Results: Coherently with the aims, the key results highlight the approaches’ diffusion in the multi-theoretical, multi-countries and multi-language geo-cultural contexts of different continents, by investigating relevant relationship between a set of meta-data - including the type of publications; bibliometric indexes (Impact Factor and SJR) of the journals; authors’ institutional affiliation countries and continents, inter- institutional collaborations (inter-continental and intracontinental), language, and evolution by decades – and the data detected through the meta-theoretical analysis – including constructs, theories, functions and main processes related to Social Representations Theory, the link with other construct, theories and disciplinary fields, the methodological profile, the preferred thematic areas and domain of applications
Comparative Analysis of Ontologies for Archival Representation
No full textOntologies are valuable tools for structuring and organizing knowledge, improving communication and data integration, enhancing information retrieval and search, and supporting various applications in different fields. They play a crucial role in promoting shared understanding and knowledge management in complex and information-rich domains. An examination of the initiatives conducted over the past decade in the field of cultural heritage has clearly highlighted the lack of an established framework in the conceptual modeling of information resources, despite the numerous ontologies created as a function of the many projects for the publication of linked open data. As a result, it is far from easy to know exhaustively all the ontologies available in relation to one's field of interest and to obtain in an easy and systematic way a reliable assessment about their representative capacity and their degree of semantic interoperability
Vertical Interoperability in Mobile Ecosystems: Will the DMA Deliver (What Competition Law Could Not)?
No full textThe metrics of the DMA's success
No full textThe Digital Markets Act (DMA) is a rare bird in competition policy. Indeed, it is a hybrid framework incorporating the institutional setting of a regulatory tool as well as the conduct already targeted by antitrust authorities in proceedings against digital platforms. From a policy perspective, the DMA seeks to prevent some anticompetitive practices. To this end, the EU legislator has construed an intricate set of provisions pursuing different policy goals. After setting out these goals in relation to the proclaimed legal interests protected by the DMA (ie, contestability and fairness), the paper uncovers the policy goals underlying each of the provisions. Relying on the first round of compliance reports issued by gatekeepers in March and October 2024, the analysis aims at providing adequate pathways to measure the DMA’s success, based on the explicit legal interests and implict policy goals fleshed out by the regulation. The paper maps out market scenarios where policymakers can assert that the DMA’s enforcement has been effective.Ciencias Jurídica
Blockchain Technologies for Digital Archives
No full textThe increase in the number of digital archives, together with the decrease of paper-based story records, profoundly changes the traditional processes of access, learning, and use of information, which pose new challenges to scientific research in order to preserve such historical information, to ensure that it is never modified, is original, and can be accessed in the future. Blockchain technology can solve these problems by providing record keeping that is both unchangeable and transparent, which can prevent the history from being compromised and ensure that authenticity reaches future generations. The paper presents a decentralized solution based on blockchain technology to eliminate the limitations of centralized data systems and to provide robust, long-term security and reliability for digital archives
The Author/Translator Interactional Process. A Case Study
No full textSee Naples and Kill (1988) is a lively and colourful novel by the con-temporary English writer, Gregory Dowling, translated into Italian in 2015.
Following the tradition of translation studies (Venuti 2000, Bass-nett 2002, Cronin 2006), this paper analyses the rewriting process of literary translation, considering in particular the fruitful but sometimes tense and even conflictual relationship between writer and translator.
The translation of the novel See Naples and Kill was an ongoing rewriting process entailing a constant dialogue between the writer and the translator. Therefore, the study aims at answering two main ques-tions: what happens if the rewriting process of translation is constant-ly questioned by the author? What happens if the author has a good mastery of the target language and s/he is her/himself a translator?
By exploring the relationship between translation and re-creation, the research focuses on the differences and similarities between the primary creation (source text) and the secondary creation (target text), and aims to verify in which way the dialogic encounter of two different personalities and cultures does not make them merge but, by retaining their own uniqueness, leads eventually to their mutually en-riching each other. A comparative analysis of the source text and the different drafts of the translated version accompanied by the author’s comments will shed light on the tense author-translator relationship in the specific case under investigation and how both actors handle this tension in order to create a new work resulting from the (dis)agreement of the two parties
Biomonitoraggio dei metalli pesanti a tutela della biodiversità e dell’alimentazione. Un caso di studio: i polli biologici della Basilicata
No full textOn Hurwicz preferences in Psychological Games
No full textThe literature on strategic ambiguity in classical games provides generalized notions of equilibrium in which each player best responds to ambiguous or imprecise beliefs about his opponents’ strategy choices. In a recent paper, strategic ambiguity has been extended topsychological games, by taking into account ambiguous hierarchies of beliefs and maxmin preferences. Given that this kind of preference seems too restrictive as a general method to evaluate decisions, in this paper we extend the analysis by taking into account α-maxmin preferences in which decisions are evaluated by a convex combination of
the worst-case (with weight α) and the best-case (with weight 1−α) scenarios. We give the definition of α-maxmin Psychological Nash Equilibrium; an illustrative example shows that the set of equilibria is affected by the parameter α and the larger is ambiguity the greater is the effect. We also provide a result of stability of the equilibria with respect to perturbations that involve the attitudes toward ambiguity, the structure of ambiguity and the payoff functions: converging sequences of equilibria of perturbed games converge to equilibria of the unperturbed game as the perturbation vanishes. Surprisingly, a final
example shows that existence of equilibria is not guaranteed for every value of α
- …
