548 research outputs found
Exosomes as a vehicle for alpha-Synuclein toxic species propagation in alpha±-synucleinopathies
No full textalpha-synucleinopathies are a group of neurodegenerative disorders characterized by the presence of abnormally aggregated alpha-synuclein (aS). Recent evidence suggests that the early site of aS aggregation is synapses, where aS seems to play its physiological role. Moreover, aggregated aS is reported to be secreted by cells, suggesting its potential involvement in disease initiation and progression. Considering the nature of neurodegenerative disorders as well as the defined, step-wise spreading of Lewy body pathology in alpha-synucleinopathies, the idea of extracellular aS as a pathogenic prion-like agent is extremely appealing. This research project developed in this frame and it is focused on the propagation of aS toxic species mediated by a particular type of extracellular vesicles, exosomes. To this aim, exosomes containing aS and DOPAL modified aS oligomers were purified by HEK293T cells and we focused on the effect of these vesicles on different cell types. Since, in neurons, exosomes appear to be secreted in a spatially and regulated manner through synapses, we first investigate their effect on primary neuronal culture synapses . Upon incubation, aS containing exosomes and, more significantly, DOPAL-modified aS containing exosomes are delivered to synapses, where they alter proteins amounts, function and neuronal morphology. aS containing exosomes contribute also to neuroinflammation: they mediate increment of IL-1beta cytokine production in mouse immortalized microglia cells. Our results highlight an exosomes-driven toxicity of aS not only to neuronal synapses, but also to microglia, inducing the secretion of IL-1beta. Therefore, aS containing exosomes appear as a vehicle of aS toxicity, which might be interesting not only as a future therapeutic target, but also as a potential biomarker for alpha-synucleinopathies
«Devi stare attento quando lavori». Sfruttamento, salute e accesso alle cure dei lavoratori stranieri in agricoltura
No full textL'articolo affronta il tema dei percorsi di prevenzione e di accesso alle cure dei lavoratori stranieri occupati in agricoltura in Toscana; in particolare, si sofferma sulle conseguenze dello sfruttamento del lavoro sul benessere dei migranti più fragili e vulnerabili che svolgono le mansioni più gravose, pericolose e disprezzate. Dopo aver tracciato il quadro di riferimento relativo ai rischi di infortunio e alle condizioni di salute dei lavoratori stranieri in Toscana e dopo aver descritto l’impianto metodologico della ricerca, l’articolo si concentra sui principali risultati emersi prestando specifica attenzione a tre dimensioni: al rischio di depauperamento del capitale di salute, anche ma non solo per cause lavoro correlate; alla salute come “fatto privato” del lavoratore e alla carenza di reti formali e istituzioni capaci di una presa in cura tempestiva ed efficace; ai limiti e alle opportunità della medicina del lavoro nell’intervento rivolto ai migranti più vulnerabili.The article addresses the topic of prevention and access to care paths for foreign workers employed in agriculture in Tuscany; in particular, it focuses on the consequences of labor exploitation on the well-being of the most fragile and vulnerable migrants who carry out the most dirty, difficult, and dangerous jobs. After outlining the risk of injury and health conditions of foreign workers in Tuscany and after describing the research methodological framework, the article focuses on the main results that emerged with specific attention to three dimensions: the risk of depletion of health capital, also but not only due to work-related causes; health as a worker’s "private fact" and the lack of formal networks and institutions for timely and effective care; the limits and opportunities of occupational medicine in interventions aimed at the most vulnerable migrants
ANALYSIS OF QUALITY DEVELOPMENT IN BROAD-LEAVED TREE-FARMING PLANTATIONS IN NORTHERN-EAST ITALY
No full textThanks to the financial incentives available through the EEC Regulation 2080/92, almost 250 hectares of tree-farming plantations have been established on previous agricultural lands in Gorizia province (northern-east Italy). The main reasons of such a policy were the excess of agricultural productions and the lack of local wood supply. The amount of subsidies was significant but the rules enforced were not sufficiently strict and properly defined; as a consequence, several management problems occurred already during the seasons after planting. The aim of this study is to assess the present quality and the quality development of the tree farming plantations that were established in Gorizia province thanks to the public funds available through the EEC Regulation 2080/92. This study has included field visits, collection of sample data representative of the entire
population and primary data analysis. Two methods of stem quality assessment have been adopted, namely Nosenzo’s classification (Nosenzo et al. 2008) and Canesin’s classification (2006). Both methodologies demonstrated to be quickly applied in the field but a statistical test was carried out to compare their outcomes. The results show the significant difference between Nosenzo’s and Canesin’s classifications
Digitising Health Research and Evolving Inequalities: Where Does the EU Stand?
No full textBig data and information technology are at the basis of great transformations in the ways health care is performed and in the way health services and products are delivered. Among these transformations one of the most challenging ones is related to the digitization and datification of health research patterns.
The proliferation of health-related apps and other software is extending the range of the tools relevant for the conduction of research. This is in turn also causing the increasing relevance of digital datasets for the design and testing of new health-related products, such as pharmaceuticals.
This contribution demonstrates that these technological developments are provoking mutations in the health research environment, raising new risks for the final consumers/patients enjoying the outputs of such research, in terms of new health products and services. As will be shown, these risks primarily relate to the occurrence of new biases affecting health research and the emergence of new stigmatizing patterns. These biases ultimately risk to negatively affect the resulting digital health products’ and services’ accessibility.
The traced scenario shows the need of an adequate regulatory governance of the currently developing datafied and digitized research environment, capable of effectively addressing the identified concerns. For these purposes, the suitability of the current European framework regarding health research, provided by the General Data Protection Regulation and the Clinical Trials Data Regulation is questioned. Ultimately, the study identifies the relevant responses currently provided by the existing normative framework, the emerging policy developments and persisting gap
TECNICHE DI RECUPERO E RESTAURO DELL'EDILIZIA -Sussidio didattico dell'Insegnamento di- http://w3.uniroma1.it/bertirestauro/
No full textMateriale sussidiario alle lezioni frontali, suddiviso in 12 Unità didattiche e proposto agli studenti dell'insegnamento di Tecniche di Recupero e restauro dell'edilizia nella Facoltà di Architettura Valle Giulia nell'Università La Sapienza di Roma. Prima edizione elaborata e distribuita a mezzo web (http://w3.uniroma1.it/bertirestauro/) e su supporto digitale (CD) negli anni accademici 2001-02, 2002-03, 2003-04, 2004-05, 2005-06, 2006-07, 2007-2008
CAMPUS di PIETRALATA-Comparto 1
No full textL'università "SAPIENZA"tramite i suoi dipartimenti di Facoltà di Architettura Valle Giulia progetta l'università nuova sede dipartimenti a PIETRALATA ROMA
Digitising Health Research and Evolving Inequalities: Where Does the EU Stand?
No full textBig data and information technology are at the basis of great transformations in the ways health care is performed and in the way health services and products are delivered. Among these transformations one of the most challenging ones is related to the digitization and datification of health research patterns.
The proliferation of health-related apps and other software is extending the range of the tools relevant for the conduction of research. This is in turn also causing the increasing relevance of digital datasets for the design and testing of new health-related products, such as pharmaceuticals.
This contribution demonstrates that these technological developments are provoking mutations in the health research environment, raising new risks for the final consumers/patients enjoying the outputs of such research, in terms of new health products and services. As will be shown, these risks primarily relate to the occurrence of new biases affecting health research and the emergence of new stigmatizing patterns. These biases ultimately risk to negatively affect the resulting digital health products’ and services’ accessibility.
The traced scenario shows the need of an adequate regulatory governance of the currently developing datafied and digitized research environment, capable of effectively addressing the identified concerns. For these purposes, the suitability of the current European framework regarding health research, provided by the General Data Protection Regulation and the Clinical Trials Data Regulation is questioned. Ultimately, the study identifies the relevant responses currently provided by the existing normative framework, the emerging policy developments and persisting gap
- …
