1,720,959 research outputs found
Peach (Prunus persica L.)
No full textUntil now, the application of genetic transformation techniques in peach has been limited by the difficulties in developing efficient regeneration and transformation protocols. Here we describe an efficient regeneration protocol for the commercial micropropagation of GF677 rootstock (Prunus persica × Prunus amygdalus). The method is based on the production, via organogenesis, of meristematic bulk tissues characterized by a high competence for shoot regeneration. This protocol has also been used to obtain GF677 plants genetically engineered with an empty hairpin cassette (hereafter indicated as hp-pBin19), through Agrobacterium tumefaciens-mediated transformation. After 7-8 months of selection on media containing kanamycin, we obtained two genetically modified GF677 lines. PCR and Southern blot analyses were performed to confirm the genetic status
Regeneration and genetic transformation via organogenesis of different cultivars of Vitis vinifera and Prunus persica
No full textFruit production represents one of the most important sectors for the Mediterranean area, including fruits for fresh market and industrial processing. The genetic transformation of fruit trees provides huge potential for the improvement of fruit production and quality and for obtaining cultivars with increased resistance to pathogens. Furthermore, efficient methods of fruit tree transformation would facilitate gene function studies. The aim of this research is to develop efficient regeneration and genetic transformation protocols to be applied to different cultivars and rootstocks of grapevine and peach by implementing a transformation method, developed for table grape and based on organogenesis. The method is used for introducing into grapevine and peach plants genetic constructs that elicit post transcriptional gene silencing (PTGS) in order to induce virus resistance or to study gene function. The regenerated lines are currently subjected to molecular analysis to verify the transgenic state
Strategies for the induction of PPV resistance through gene silencing in cultivars and rootstocks of Prunus spp.
No full textAn innovative and efficient regeneration and genetic transformation protocol developed for grapevine is applied to different fruit species as peach and apricot. This method is used for the obtainment of virus resistance to PPV by the introduction of constructs capable of inducing a mechanism of post-transcriptional gene silencing (PTGS)
Peach (Prunus Persica L.)
No full textUntil now, the application of genetic transformation techniques in peach has been limited by the difficultiesin developing efficient regeneration and transformation protocols. Here we describe an efficient regenerationprotocol for the commercial micropropagation of GF677 rootstock (Prunus persica × Prunusamygdalus).The method is based on the production, via organogenesis, of meristematic bulk tissues characterizedby a high competence for shoot regeneration.This protocol has also been used to obtain GF677 plants genetically engineered with an empty hairpincassette (hereafter indicated as hp-pBin19), through Agrobacterium tumefaciens-mediated transformation.After 7–8 months of selection on media containing kanamycin, we obtained two genetically modifiedGF677 lines. PCR and Southern blot analyses were performed to confirm the genetic status
Biotecnologie alternative: silenziamento genico post-trascrizionale per indurre resistenza a Sharka nelle drupacee
No full textLe drupacee, in particolare il pesco (Prunus persica), rappresentano le
specie da frutto coltivate più importanti del bacino del Mediterraneo
e anche in Italia, ma sono soggette a molte perdite agronomiche ed economiche causate da infezioni virali. Una delle malattie più virulente è rappresentata dalla Sharka, causata dal “Plum Pox Virus” (PPV). Al momento non ci sono mezzi di lotta diretta contro tali infezioni, ma solo mezzi di prevenzione che causano grossi problemi di sostenibilità
ambientale ed enormi perdite di reddito per gli agricoltori. Le tecniche di trasformazione genetica potrebbero essere impiegate anche per introdurre geni di resistenza individuati in Prunus spp. o geni per il silenziamento genico post-trascrizionale (PTGS) capaci di indurre resistenza
a virus. Quest’ultima tecnologia può essere utilizzata per introdurre resistenza alla Sharka in varietà e portinnesti di pesco e delle altre principali drupacee
Regeneration and genetic transformation via organogenesis of different cultivars of Vitis vinifera and Prunus persica
No full textFruit production represents one of the most important sectors for the Mediterranean area, including fruits for fresh market and industrial processing. The genetic transformation of fruit trees provides huge potential for the improvement of fruit production and quality and for obtaining cultivars with increased resistance to pathogens. Furthermore, efficient methods of fruit tree transformation would facilitate gene function studies. The aim of this research is to develop efficient regeneration and genetic transformation protocols to be applied to different cultivars and rootstocks of grapevine and peach by implementing a transformation method, developed for table grape and based on organogenesis. The method is used for introducing into grapevine and peach plants genetic constructs that elicit post transcriptional gene silencing (PTGS) in order to induce virus resistance or to study gene function. The regenerated lines are currently subjected to molecular analysis to verify the transgenic state
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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