1,720,992 research outputs found
Importance of showing HBsAg and HBcAg positivity in the liver for better aetiological definition of chronic liver disease
No full textCo-expression of two Helicobacter pylori’s proteins CagA and HspB induces cell proliferation in gastric epithelial cells, independently from the bacterial infection
No full textRole of HspB and CagA in molecular pathogenesis of Helicobacter pylori-positive gastric cell
No full textAdenocarcinoma of the stomach is one of the most common cause of cancer mortality in the world. H. pylori has been implicated in gastric carcinogenesis only on the basis of epidemiological studies. However, direct experimental evidence demonstrating a causal role for H. pylori in carcinogenesis has been lacking. Only recently it has been demonstrated that long-term infection with H. pylori induces adenocarcinoma in mongolian gerbils. Aim of the work was to evaluate the effects of two H. pylori proteins, CagA and HspB, on cell kinetics and the ability to selectively affect the expression of cell cycle related proteins using epithelial cell line (AGS). Using a genomic library of H. pylori we were able to isolate and clone in a pcDNA3 vector two proteins expressed from the H. pylori CagA and HspB. These proteins were overexpressed in a gastric cell line (AGS). The effects of these proteins on the proliferation rates of the cells transfected were analyzed by poliferation assay. Immunoblots screening was performed on cell lysates with antibodies against the major proteins known to regulate the cell cycle checkpoints. Moreover the same cells were analyzed by flow cytometry. After over expression, in AGS cells of CagA and HspB, we observed an increase of proteins involved on the G1/S checkpoint. We demonstrated that the process of transformation from normal gastric mucosa to carcinoma is characterized by increased cell proliferation. Proteins expressed from H. pylori may act as a promoter in gastric carcinogenesis by causing a state of increased gastric epithelial proliferation. This data taken together provide additional information to further enhance our understanding of the molecular mechanism by which H. pylori proteins alter the growth status of the cell
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Effects of the interaction between Helicobacter pylori and eukaryotic proteins in gastric cancer
No full text[Tissue markers of hepatitis B virus: useful indicators in the etiologic diagnosis of chronic alcoholic and non-alcoholic liver disease]
No full textVariations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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