182 research outputs found
Collapse and Reconstitution: Autonomy and the Avant-Garde
No full textA critical discussion of the historical avant-garde, De Stijl and the work of Theo van Doesburg and its reception and re-interpretations through the years. For the occasion of an art installation at the Centraal Museum in Utrecht by the artist Antonis Pittas.Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Space & Typ
Molecular-genetic analysis of natural variation in photoperiodic flowering of Arabidopsis thaliana
No full textIn Arabidopsis thaliana, the focus of my research, three developmental switches controlling the life cycle can be recognised. The first is germination that separates embryonic from post-embryonic development. The second signals the transition from the juvenile to the adult vegetative phase while the third, flowering, marks the initiation of the reproductive phase (Isabel Baurle and Caroline Dean, Cell 2006). All three exhibit both external (environmental) and endogenous (hormones) regulation. Natural genetic variation, namely phenotypic diversity due to genetic differences between individuals of the same species, has been reported both for germination and flowering initiation (Bentsink et al., PNAS 2006; O Neill et al., TAG 2008). Since individuals of Arabidopsis, commonly referred to as accessions, are collected from a variety of locations, it is believed that this genetic diversity reflects differences in the seasonal oscillations of environmental cues among the collection sites leading to local adaptation. Although natural genetic variation as a tool has been used in the study of flowering initiation in Arabidopsis (Alonso-Blanco and Maarten Koornneef, Trends in Plant Science 2000) a systematic survey that focuses mainly on the photoperiodic aspect of this regulation has been lacking. In order to expand the current knowledge two approaches were designed. First a survey for natural genetic variation in the flowering responses of phylogenetically distant Arabidopsis accessions under six different photoperiods was made. In parallel the transgenic equivalents of the same accessions, carrying a promoter fusion of the flowering time and circadian clock gene GIGANTEA (GI) were screened in the same photoperiods as for flowering time in order to detect for the first time trans-specific natural variation in the circadian regulation of an evening gene. Here I present evidence that natural genetic variation is present in a wide range of photoperiods both for the circadian clock and for flowering initiation per se. The flowering time responses are compared with the ones of mutants and transgenic lines of previously identified flowering time genes and I show that the affected known genes cannot fully cover the different patterns of day length discrimination that the natural accessions exhibit. Five different mapping populations were constructed by selecting interesting accessions from both screens, which led to the identification of new as well as known QTL, which alter various circadian and flowering responses between short and long days of similar duration. Generating advanced genetic material allows fine mapping and eventually cloning of some of the loci, while identification of genome-wide patterns of genetic interactions reveals additional loci that classical QTL mapping approaches cannot detect. Using RT-PCR and in situ hybridisation, I link this novel natural genetic variation between similar long day lengths with molecular variability in the temporal and spatial expression of flowering time genes FT and SOC1 thereby also demonstrating the tight dependence of the SAM floral commitment on the FT florigen. Finally I show that in nature, genetic variability in the property of enhanced photoperiod discrimination under similar long days, is enough to prevent winter flowering in a plant without any requirements for vernalization. Cologne, 200
Corrigendum to “Functional and immunohistochemical characterization of CCEae3a, a carboxylesterase associated with temephos resistance in the major arbovirus vectors Aedes aegypti and Ae. albopictus” [Insect Biochem. Mol. Biol. 74 (July 2016) 61–67]
No full textThe authors regret that the name of the fourth author was given incorrectly. The correct name should be Antonis Myridakis instead of Antonis Miridakis. The authors would like to apologise for any inconvenience caused
Viruses affecting lentil (Lens culinaris Medik.) in Greece; incidence and genetic variability of Bean leafroll virus and Pea enation mosaic virus
No full textIn Greece, lentil (Lens culinaris Medik.) crops are mainly established with non-certified seeds of local landraces, implying high risks for seed transmitted diseases. During April and May of the 2007-2012 growing seasons, surveys were conducted in eight regions of Greece (Attiki, Evros, Fthiotida, Korinthos, Kozani, Larissa, Lefkada and Viotia) to monitor virus incidence in lentil fields. A total of 1216 lentil samples, from plants exhibiting symptoms suggestive of virus infection, were analyzed from 2007 to 2009, using tissue-blot immunoassays (TBIA). Pea seed-borne mosaic virus (PSbMV) overall incidence was 4.9%, followed by Alfalfa mosaic virus (AMV) (2.4%) and Bean yellow mosaic virus (BYMV) (1.0%). When 274 of the samples were tested for the presence of luteoviruses, 38.8% were infected with Bean leafroll virus (BLRV). Since BLRV was not identified in the majority of the samples collected from 2007 to 2009, representative symptomatic plants (360 samples) were collected in further surveys performed from 2010 to 2012 and tested by ELISA. Two viruses prevailed in those samples: BLRV (36.1%) was associated with stunting, yellowing, and reddening symptoms and Pea enation mosaic virus-1 (PEMV-1) (35.0%) was associated with mosaic and mottling symptoms. PSbMV (2.2%), AMV (2.2%), BYMV (3.9%) and CMV (2.8%) were also detected. When the molecular variability was analyzed for representative isolates, collected from the main Greek lentil production areas, five BLRV isolates showed 95% identity for the coat protein (CP) gene and 99% for the 3' end region. Three Greek PEMV isolates co-clustered with an isolate from Germany when their CP sequence was compared with isolates with no mutation in the aphid transmission gene. Overall, limited genetic variability was detected among Greek isolates of BLRV and PEMV
How close before you burn? Questions of ethics and distance in researching crisis and unrest
No full textResearchers examining urban riots or unrest constantly face questions about the motivations behind and impact of their work. These questions verge on the existential, because questioning a research topic essentially interrogates researchers’ role and existence as social scientists. With reference to two project examples from Athens, Greece, the author attempts to show how he has so far tried to grapple with such questions. Researching closely and drawing conclusions from distance: this has been a personal model of adjustable distance to the author’s research subjects, a strategy that seem to have somehow worked for the time being, writes Antonis Vradis
Dataset: "I Can't Keep It Up." A Dataset from the Defunct Voat.co News Aggregator
No full textThis is the dataset released with the paper titled: "I Can’t Keep It Up." A Dataset from the Defunct Voat.co News Aggregator.
The dataset consists of 15,133 Newline delimited JSON files (ndjson). More specifically, 7,616 files for submission data, 7,515 for comment data, 1 for user data, and 1 for subverse data. Each line in the ndjson files consists of a JSON object. The JSON objects contain all the key/values we collect through the Voat API and the custom parser of the Internet Archive Wayback Machine Voat snapshot release.
For the detailed description of every key in the JSON structure, along with the type of the value, please read the readme.pdf file provided with this dataset.
If you find our dataset useful, please cite our paper:
@inproceedings{mekacher2022can,
title={"I Can't Keep It Up." A Dataset from the Defunct Voat.co News Aggregator},
author={Mekacher, Amin and Papasavva, Antonis},
booktitle={16th International Conference on Web and Social Media},
year={2022}
Antonis Samaras: The Permanent Campaign Strategy of a Centre-Right Prime Minister
No full textThis chapter focuses on the analysis of Antonis Samaras’ political background and campaigning style of governing in the era of sovereign debt crisis and the implementation of the memoranda. It measures and assesses permanent campaigning of Samaras against the three categories of the proposed theoretical framework (capacity building and strategy, paid and owned media as well as earned media). It finds that as prime minister and in line with his predecessors did follow the permanent campaign trend though he did not use all its features. He put more emphasis on factors related to capacity building and strategy as well as earned media rather than most of the components of the paid and owned media that focused on the use of social media, except for the election themes and negative campaigning. © 2022, The Author(s), under exclusive license to Springer Nature Switzerland AG
RNA-Independent Regulatory Functions of lncRNA in Complex Disease
No full textDuring the metagenomics era, high-throughput sequencing efforts both in mice and humans indicate that non-coding RNAs (ncRNAs) constitute a significant fraction of the transcribed genome. During the past decades, the regulatory role of these non-coding transcripts along with their interactions with other molecules have been extensively characterized. However, the study of long non-coding RNAs (lncRNAs), an ncRNA regulatory class with transcript lengths that exceed 200 nucleotides, revealed that certain non-coding transcripts are transcriptional “by-products”, while their loci exert their downstream regulatory functions through RNA-independent mechanisms. Such mechanisms include, but are not limited to, chromatin interactions and complex promoter-enhancer competition schemes that involve the underlying ncRNA locus with or without its nascent transcription, mediating significant or even exclusive roles in the regulation of downstream target genes in mammals. Interestingly, such RNA-independent mechanisms often drive pathological manifestations, including oncogenesis. In this review, we summarize selective examples of lncRNAs that regulate target genes independently of their produced transcripts
<i>Cucumber mosaic virus</i> Isolates from Greek Legumes are Associated with Satellite RNAs that are Necrogenic for Tomato
No full text Worldwide, Cucumber mosaic virus (CMV) is the causal agent of many economically important diseases. Based on immunological or molecular analysis, three distinct subgroups of CMV isolates can be identified (IA, IB, and II). In addition, some CMV isolates are associated with satellite RNAs (satRNAs), a type of noncoding transcript that may alter the symptoms of CMV infections. This study presents an analysis of CMV isolates occurring in legumes in Greece in respect to their genetic diversity, and the presence and diversity of their satRNA. Phylogenetic analysis of the CMV coat protein sequence of 18 legume and 5 tomato CMV isolates collected throughout Greece classified them within subgroups IA and IB, with a limited genetic diversity. The CMV satRNAs found in nine field legumes exhibiting mild symptoms and in one tomato with a necrotic syndrome contained a functional necrogenic motif; therefore, they were grouped within the necrogenic group of CMV-satRNAs. The necrotic phenotype was expressed in all legume CMV isolates containing necrogenic satRNAs when mechanically inoculated onto tomato plants. To our knowledge, this is the first observation that legumes host necrogenic CMV-satRNAs. The possible role of legumes in the epidemiology of CMV and necrogenic satRNA complex is discussed. </jats:p
Correction to: Assessing environmental impacts through innovative solutions (Environmental Science and Pollution Research, (2022), 29, 20, (29524-29527), 10.1007/s11356-022-19068-3)
No full textThe correct given name of the 2nd Author is Antonis. The Original article has been corrected © Springer-Verlag GmbH Germany, part of Springer Nature 2022
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