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Dexamethasone and Stanozolol affect the expression of genes related to osteogenic differentiation in SaOS-2 cell line
No full textPlacental stem cells are a useful tool for bone regeneration with biomimetic scaffolds: the actors perform better if the setting is appropriate
No full text
Hyperhydrophilic surfaces enhance the effects of oxidative stress on bone cells and LiCl can reverse them
No full textDexamethasone and Stanozolol affect osteogenic differentiation of SaOS-2 cells.
No full textAim: The aim of this study is to investigate the effects of
Dexamethasone (DX) and Stanozolol (ST) in inducing osteogenic
differentiation on SaOS-2 cells.
Material and Methods: Cells were cultured in DMEM-low
glucose supplemented with Fetal Bovine Serum 10%, penicillin/
streptomicin 100 lg/ml, glutammin 4 mmol/l, ascorbic acid
50 lg/ml, L-proline 260 lmol/l, b2-glicerophosphate 10 mmol/
l. Either Dexamethasone or Stanozolol at concentrations of 0, 1,
10, 100, 1000 nmol/l were furtherly added as experimental conditions.
After 6, 12 and 24 days, cells were stained with Alizarin
Red (AR), Von Kossa (VK) for qualitative analysis and with
DAPI and calcein green for semi-quantitative analysis. Gene
expression of RUNX-2 and BMP-1 was evaluated through RTPCR.
Results: AR and VK stainings showed a dose-dependent mineral
apposition in cells treated with ST. This finding was more
evident at 12 days, while at 24 days all samples treated with ST
were extensively calcified. Semi-quantitative evaluation of calcein/
DAPI confirmed a dose-dependant mineralization even at
the last time-point. Samples treated with DX exhibited similar
results, with a less pronounced mineral apposition. Gene expression
analysis revealed a dose-dependant increase of Runx-2 in
samples treated with ST compared to controls (p < 0.05), and
not significant changes in DX-treated samples at any tested concentration
(p > 0.05). BMP-1 expression had a significant dosedependant
decrease in samples treated with DX (p < 0.05).
Conclusion: Standing to our results, ST boosts osteogenic differentiation
of SaOS-2 in a dose-dependent manner. Also DX
may produce similar effects, at a lower rate. Further studies are
required to understand steroids’ mechanism of action on osteogenic
cells as well as their possible use in the field of bone
regeneration
Tailoring the Interface of Biomaterials to Design Effective Scaffolds
Get PDFTissue engineering (TE) is a multidisciplinary science, which including principles from material science, biology and medicine aims to develop biological substitutes to restore damaged tissues and organs. A major challenge in TE is the choice of suitable biomaterial to fabricate a scaffold that mimics native extracellular matrix guiding resident stem cells to regenerate the functional tissue. Ideally, the biomaterial should be tailored in order that the final scaffold would be (i) biodegradable to be gradually replaced by regenerating new tissue, (ii) mechanically similar to the tissue to regenerate, (iii) porous to allow cell growth as nutrient, oxygen and waste transport and (iv) bioactive to promote cell adhesion and differentiation. With this perspective, this review discusses the options and challenges facing biomaterial selection when a scaffold has to be designed. We highlight the possibilities in the final mold the materials should assume and the most effective techniques for its fabrication depending on the target tissue, including the alternatives to ameliorate its bioactivity. Furthermore, particular attention has been given to the influence that all these aspects have on resident cells considering the frontiers of materiobiology. In addition, a focus on chitosan as a versatile biomaterial for TE scaffold fabrication has been done, highlighting its latest advances in the literature on bone, skin, cartilage and cornea TE
Local delivery of Stanozolol enhances new bone formation in rat calvarial critical-size defects
No full textLo Stanozololo caricato su scaffold di osso bovino deproteinizzato promuove la rigenerazione ossea nel ratto
No full textModello di innesto a blocco di tipo “over-inlay” per lo studio della rigenerazione ossea nel ratto
No full textObiettivo: L’obiettivo di questo studio è di descrivere un modello di innesto a blocco per la valutazione della rigenerazione ossea nel ratto. Materiali e Metodi: Difetti critici standardizzati dal diametro di 5 mm sono stati creati nell’osso parietale di 12 ratti Wistar maschi di 4 mesi. Blocchi di osso bovino deproteinizzato sono stati inseriti all’interno dei difetti, in modo che una parte del blocco fosse alloggiata nella cavità chirurgica (parte “inlay”) ed una parte eccedesse in altezza lo spessore della teca cranica esterna (parte “over”). Sei animali sono stati sacrificati a 1 mese e 6 a 3 mesi e all’interno di ogni campione sono state identificate diverse regioni di interesse (ROI): area periostale (PA), aree laterali adiacenti all’osso nativo (BA), aree centrali (CA). In ogni ROI sono stati quantificati osso neoformato (NB) e tessuto fibroso (FT). Densità di capillari, espressione di Osterix (OSX) e collagene1 (COL1) sono state valutate con immunoistochimica. I dati sono stati analizzati tramite test 2way-ANOVA e post-test di Sidak, p ≤ 0.05. I risultati sono espressi come media ± SEM. Risultati: NB è risultato maggiore in BA rispetto a CA, a 1 mese (42183.89±6477.27 μm2, p< 0.05) e a 3 mesi (64131.11±7520.61 μm2, p<0.01). FT è risultato maggiore in PA rispetto alle altre ROI a 1 mese (PA 179192.50±29187.19 μm2, p<0.01) e 3 mesi (243367.80±55447.25 μm2, p<0.001). I dati sulla densità capillare hanno evidenziato una progressiva migrazione del processo di neoangiogenesi da BA verso CA. Positività per OSX e COL1 è stata riscontrata soprattutto in PA. Conclusioni: Il modello “over-inlay” rende possibile un approccio critico per la valutazione del contributo distinto di diverse ROI nel processo di integrazione dell’innesto: la parte inlay consente di valutare BA e CA, la parte “over,” priva dell’apporto dell’osso nativo, la PA. Questo consente di ottenere dati più facilmente interpretabili con conseguente riduzione della numerosità del modello animale
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