1,721,005 research outputs found
Preliminary studies on the effect of processing on the allergenicity of tomato products.
No full textApplication of new specific probes in ion-exchange hplc analysis of DNA for the detection of GMO in foods
No full textSviluppo di nuovi metodi per il monitoraggio di living modified organisms attraverso la filiera alimentare
No full textDevelopment of a Peptide Nucleic Acid/HPLC method for the detection of traces of potentially allergenic hazelnut in foodstuff
No full text
Simultaneous detection of Escherichia coli O175:H7, Salmonella spp., and Listeria monocytogenes by multiplex PCR
No full textThe wide application of nucleic acid amplification techniques and the increasing industrial interest
toward rapid methods has led to the development and application of PCR based methods for the detection
of microbial pathogens in food. In the present paper we describe the development of a multiplex PCR
method for simultaneous detection of Salmonella enterica serovar Typhimurium, Listeria monocytogenes
and Escherichia coli O157:H7 in a complex food matrix (liquid whole egg).
Four different DNA extraction procedures were evaluated for their application on food and, among
these, Chelex resin combined with a DNA purification step were found to better perform on the food system
considered.
A multiplex PCR system was developed, based on the evaluation and combination of published primer
sets, and applied to the simultaneous detection of the target pathogens plus an internal amplification
control, both in culture media and in a model food system.
The overall system proposed, based on an overnight enrichment step followed by DNA isolation and
multiplex PCR, was satisfactorily tested for its specificity and sensitivity and allowed the detection of
the presence of bacterial DNA and the identification of the target pathogens down to 10 cells/25 g liquid
whole egg
Investigation on the occurrence of transgenic material on the italian food market by a new multiplex PCR method
No full text- …
