2,512 research outputs found
Dimer-dimer stacking interactions are important for nucleic acid binding by the archaeal chromatin protein Alba
No full textArchaea use a variety of small basic proteins to package their DNA. One of the most widespread and highly conserved is the Alba (Sso10b) protein. Alba interacts with both DNA and RNA in vitro, and we show in the present study that it binds more tightly to dsDNA (double-stranded DNA) than to either ssDNA (single-stranded DNA) or RNA. The Alba protein is dimeric in solution, and forms distinct ordered complexes with DNA that have been visualized by electron microscopy studies; these studies suggest that, on binding dsDNA, the protein forms extended helical protein fibres. An end-to-end association of consecutive Alba dimers is suggested by the presence of a dimer-dimer interface in crystal structures of Alba from several species, and by the strong conservation of the interface residues, centred on Are and Phe(60). In the present study we map perturbation of the polypeptide backbone of Alba upon binding to DNA and RNA by NMR, and demonstrate the central role of Phe(60) in forming the dimer dimer interface. Site-directed spin labelling and pulsed ESR are used to confirm that an end-to-end, dimer dimer interaction forms in the presence of dsDNA.Peer reviewe
First person – Alba Delrio-Lorenzo
No full textFirst Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Alba Delrio-Lorenzo is first author on ‘Sarcoplasmic reticulum Ca2+ decreases with age and correlates with the decline in muscle function in Drosophila’, published in JCS. Alba is a PhD student in the lab of Javier García-Sancho and María Teresa Alonso at the Instituto de Biología y Genética Molecular (IBGM), University of Valladolid, Spain, investigating the molecular mechanisms implicated in aging, particularly muscle aging.Peer reviewe
Alerta de explosiones algales en el Embalse San Roque a partir de datos satelitales diarios y mediciones de campo
No full textTesis (Magister en Aplicaciones Espaciales de Alerta y Respuesta Temprana a Emergencias)--Universidad Nacional de Córdoba, Facultad de Matemática, Astronomía, Física y Computación, 2017.Maestría conjunta con el Instituto de Altos Estudios Espaciales "Mario Gulich"-CONAEFil: German, Alba. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía, Física y Computación; Argentina.Fil: German, Alba. Universidad Nacional de Córdoba. Comisión Nacional de Actividades Espaciales. Instituto de Altos Estudios Espaciales Mario Gulich; Argentina.La eutrofización es un fenómeno que afecta a muchos cuerpos de agua en todo el mundo. En casos severos, la eutrofización puede llevar a grandes floraciones de algas. El Embalse San Roque, ubicado en la provincia de Córdoba, Argentina, no es ajeno a este proceso y en los últimos años se ha observado un incremento en las concentraciones anuales máximas de algas como consecuencia de eventos de floracion. Frente a esto, el monitoreo de la calidad de su agua es clave ya que es la fuente de abastecimiento más importante de la ciudad de Córdoba. La teledetección es una herramienta fundamental para complementar los monitoreos in situ tradicionales y comprender los procesos que ocurren en el Embalse. En este trabajo de tesis se generó un algoritmo semi-empı́rico obtenido a partir de datos satelitales del sensor MODIS y de mediciones in situ realizadas por la Secretarı́a de Recursos Hı́dricos de la Provincia de Córdoba. A partir de este modelo se obtuvo una serie temporal de concentración de Clorofila-a entre los años 2001 y 2014. Del análisis de dicha serie se puede concluir que el estado del agua del Embalse ha empeorado en los últimos 5 años (2010-2014). respecto de los primeros cinco de la serie (2001-2005). Además, se desarrolla un método de detección de floraciones algales a partir de la serie temporal de concentración de Clorofila-a (Cl-a) entre los años 2001 y 2014. A partir de la detección de las fechas de eventos de floración y caracterización de las mismas, es posible definir el rango de valores de Cl-a en los que el Embalse San Roque está en evento de floración ası́ como su estacionalidad, lo que permite generar una alerta cuando el cuerpo de agua se encuentra en estado de floración algal, fenómeno que puede afectar la salud humana.Eutrophication is a phenomenon that affects many water bodies around the world. In severe cases, eutrophication can lead to large algae blooms. San Roque Dam, located in the province of Cordoba, Argentina, is also subject to this process and in recent years there has been a disproportionate increase in algae concentration due to bloom events. In this context, monitoring the quality of water is crucial because it is the most important source of water supply for Cordoba city. Remote sensing is a fundamental complement to traditional in situ monitoring and aids to understand the processes occurring in the dam. In this thesis a semiempirical algorithm was obtained from MODIS data and in situ chlorophyll-a measurements, provided by the Ministry of Water Resources of Cordoba Province. From this model, a daily time chlorophyll-a time series was obtained from 2001 to 2014. Statistical analysis of the series indicate that water quality of the reservoir has worsened when comparing sets of last (2014-2010) and first (2010-2014) five years. Also a method to detect algae blooms was developed based on a time series of chlorophyll-a (Chl-a) concentration in the period 2001-2014. By detecting algae bloom dates and their statistic characterization, it is possible to define the range of Chl-a values in which the San Roque Dam is going through a bloom event and its seasonality, which allows generating an alert when the dam is in bloom event, which can be a problem to human healthFil: German, Alba. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía, Física y Computación; Argentina.Fil: German, Alba. Universidad Nacional de Córdoba. Comisión Nacional de Actividades Espaciales. Instituto de Altos Estudios Espaciales Mario Gulich; Argentina
A surrogate-based multi-disciplinary design optimization framework modeling wing–propeller interaction
No full textThis paper presents a multi-disciplinary design optimization (MDO) framework for design of a general aviation aircraft wing considering the effects of tractor propellers on the wing aerodynamic characteristics. In pursuit of this objective, a wing–propeller full-interaction aerodynamic routine was developed and integrated with structural and performance models. A substantive contribution of the work is the approach for effectively modeling wing effects on propeller slipstream development while still leveraging traditional propeller and wing analysis tools. Several optimizations were carried out, starting from an existing aircraft design, to test different local and global surrogate-based optimization frameworks and to allow for the assessment of the resulting solutions and corresponding computational performance metrics. Examination of the total function calls and run times showed that the use of surrogate models improves overall optimization performance, provided that suitable surrogate modeling techniques are chosen.</p
A Surrogate-Based Multi-Disciplinary Design Optimization Framework Exploiting Wing-Propeller Interaction
No full textThis paper presents multi-disciplinary design optimization (MDO) framework for design of general aviation aircraft wing including the effect of wing mounted propeller on the wing aerodynamic characteristics. In prusuit of achieving such objective, a wing-propeller full-interaction routine was developed, and integrated in a multidisciplinary design analysis including structural and performance models.
The Author/Translator Interactional Process. A Case Study
No full textSee Naples and Kill (1988) is a lively and colourful novel by the con-temporary English writer, Gregory Dowling, translated into Italian in 2015.
Following the tradition of translation studies (Venuti 2000, Bass-nett 2002, Cronin 2006), this paper analyses the rewriting process of literary translation, considering in particular the fruitful but sometimes tense and even conflictual relationship between writer and translator.
The translation of the novel See Naples and Kill was an ongoing rewriting process entailing a constant dialogue between the writer and the translator. Therefore, the study aims at answering two main ques-tions: what happens if the rewriting process of translation is constant-ly questioned by the author? What happens if the author has a good mastery of the target language and s/he is her/himself a translator?
By exploring the relationship between translation and re-creation, the research focuses on the differences and similarities between the primary creation (source text) and the secondary creation (target text), and aims to verify in which way the dialogic encounter of two different personalities and cultures does not make them merge but, by retaining their own uniqueness, leads eventually to their mutually en-riching each other. A comparative analysis of the source text and the different drafts of the translated version accompanied by the author’s comments will shed light on the tense author-translator relationship in the specific case under investigation and how both actors handle this tension in order to create a new work resulting from the (dis)agreement of the two parties
Characters with split personalities in Nessuno torna indietro by Alba de Céspedes and the German-Polish history of the novel
No full textCharacters with split personalities in Nessuno torna indietro by Alba de Céspedes and the German-Polish history of the novel – This paper focuses on the Polish reception of Nessuno torna indietro, a novel by Alba de Céspedes. In Italy the novel was a bestseller between 1938 and the eighties, however it was impossible to publish it in Poland due to the fact that negotiations failed. Nevertheless, the book was translated into Polish on the basis of the German version and published in a newspaper in 1947.
The presentation of the Polish history of this novel will be based on archival materials
Songs of the sixteenth century
No full textInvestigation of 500 worldly songs in sixteenth-century Dutch/German Alba Amicorum and their socio-cultural context
Inkunabeln aus dem Besitz von Conrad Celtis in Alba Iulia
No full texthttp://www.mnuai.ro/apulum.php Dârja, Ileana: Patru incunabule italiene ale poetului german Conrad Celtis în colecţia Bibliotecii Naţionale a României, Filiala Batthyaneum din Alba Iulia. In: Apulum, XLV, 2008, p. 483-497 Rumänisch mit englischer Zusammenfassung: This article presents four Italian incunabula from the collection of the National Library of Romania, Batthyaneum Branch from Alba Iulia that belonged to the German poet Conrad Celtis. [...] Die Zeitschrift Apulum ist kostenfrei dort..
Inkunabeln aus dem Besitz von Conrad Celtis in Alba Iulia
No full texthttp://www.mnuai.ro/apulum.php Dârja, Ileana: Patru incunabule italiene ale poetului german Conrad Celtis în colecţia Bibliotecii Naţionale a României, Filiala Batthyaneum din Alba Iulia. In: Apulum, XLV, 2008, p. 483-497 Rumänisch mit englischer Zusammenfassung: This article presents four Italian incunabula from the collection of the National Library of Romania, Batthyaneum Branch from Alba Iulia that belonged to the German poet Conrad Celtis. [...] Die Zeitschrift Apulum ist kostenfrei dort..
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