1,721,104 research outputs found
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
mTOR transcriptional regulation by Nrf2
No full textNuclear Erythroid 2-related factor (Nrf2) is a master transcription factor, and thereby is a major regulator of cytoprotective responses to oxidative and electrophilic stress. This is accomplished by recognition and binding to antioxidant response elements (ARE) in the promoter of target genes, which triggers activation of genes encoding proteins that range from drug metabolizing enzymes II family to drug efflux pumps. Numerous studies have shown direct and indirect interactions between Nrf2 and different signaling pathways including components of the Pi3K/AKT/mTOR signaling pathway.The potential for a role for Nrf2 in cancer metabolism directed our study towards its impact on mTOR, the metabolic maestro of this pathway. We observed that modulation of Nrf2 levels in lung cancer cell lines regulates mTOR protein levels. In order to verify if this regulation is present at the transcriptional level, we performed both RT-qPCR analysis and a luciferase assay to functionally analyze the promoter region of this gene for the presence of functional ARE motifs. We found that transcription of the Mtor protein was directly modulated by Nrf2 levels in the non small cell lung cancer cell line A549, as well as in the non-transformed human cell line HEK293. Mutation of the ARE sequence in the promoter of the mTOR gene, decreased the effect of Nrf2 on an ARE- luciferase construct’s activity by more than 50%. The physical binding of Nrf2 with the ARE sequence in mTOR promoter was further confirmed in vitro via DNA pull-down and EMSA and in vivo via in a ChIP assay. Additional studies show intimate interactions between other components of the PI3K pathway and Nrf2.Nuclear Erythroid 2-related factor (Nrf2) est un facteur de transcription qui joue un rôle primordial dans la défense cellulaire contre les stress oxydatif et électrophile. Il régule la transcription en se fixant sur les éléments de réponse antioxidative (ARE) impliqués dans la résistance et le métabolisme des médicaments. En outre, plusieurs études montrent des intercactions directes ou indirectes de Nrf2 avec la voie de signalisation Pi3K/AKT/mTOREn se basant sur le rôle de Nrf2 dans le métabolisme du cancer et son interaction avec la voie de signalisation mTOR, nous avons formulé l'hypothèse selon laquelle Nrf2 régulerait les niveaux de mTOR. Tout D'abord, nous avons observé que la modulation des niveaux de Nrf2 dans les cellules du cancer du poumon régule mTOR au niveau protéique. Ensuite, l'utilisation de la PCR quantitative à temps réel et l'essai de transactivation sur un vecteur rapporteur luciférase contenant le promoteur de mTOR nous a permis de montrer que Nrf2 régule mTOR au niveau transcriptionnel dans les cellules HEK293 et A549.D'autre part, l'introduction des mutations au sein de la séquence de l'ARE du promoteur de mTOR réduit l'activité luciférase par plus de 50%. Ceci confirme que malgré sa séquence différente de la séquence consensus, cet ARE est requis pour la liaison et la régulation de l'expression de mTOR.l'interaction physique de Nrf2 avec l'ARE du promoteur de mTOR a été confirmé in vitro par DNA pull down et par retard sur gel (EMSA) et in vivo par immunoprécipitation de la chromatine. En conclusion, nos résultats suggèrent que le rôle de Nrf2 dans la sensibilité aux traitements cytotoxiques pourrait découler de sa capacité à réguler l'expression de mTOR
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
sj-docx-1-tam-10.1177_17588359221133893 – Supplemental material for Comorbidity between lung cancer and COVID-19 pneumonia: role of immunoregulatory gene transcripts in high ACE2-expressing normal lung
No full textSupplemental material, sj-docx-1-tam-10.1177_17588359221133893 for Comorbidity between lung cancer and COVID-19 pneumonia: role of immunoregulatory gene transcripts in high ACE2-expressing normal lung by Vladimir Lazar, Jacques Raynaud, Shai Magidi, Catherine Bresson, Jean-François Martini, Susan Galbraith, Fanny Wunder, Amir Onn, Gerald Batist, Nicolas Girard, Ulrik Lassen, C. S. Pramesh, Amal Al-Omari, Sadakatsu Ikeda, Guy Berchem, Jean-Yves Blay, Benjamin Solomon, Enriqueta Felip, Josep Tabernero, Eitan Rubin, Thierry Philip, Angel Porgador, Ioana Berindan-Neagoe, Richard L. Schilsky and Razelle Kurzrock in Therapeutic Advances in Medical Oncology</p
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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