1,721,026 research outputs found
QuantStudioTM 12K Flex OpenArray® System as a Tool for High-Throughput Genotyping and Gene Expression Analysis
No full textReal time technology provides great advancements over PCR-based methods for a broad range of applications. With the increased availability of sequencing information, there is a need for the development and application of high-throughput real time PCR genotyping and gene expression methods that significantly broaden the current screening capabilities. Thermo Fisher Scientific (USA) has released a platform (QuantStudioTM 12K Flex system coupled with OpenArray® technology) with key elements required for high-throughput SNP genotyping and gene expression analysis. This allows for a rapid screening of large numbers of TaqMan® assays (up to 256) in many samples (up to 480) per run. This advanced real-time method involves the use of an array composed of 3,000 through-holes running on the QuantStudioTM 12K with OpenArray® block. The aim of this chapter is to outline the OpenArray® approach while providing a comprehensive in-depth review of the scientific literature on this topic. In agreement with a large number of independent studies, we conclude that the use of OpenArray® technology is a rapid and accurate method for high-throughput and large-scale systems biology studies with high specificity and sensitivity
Presa in carico integrata e multidisciplinare dei disturbi dello spettro autistico: il caso studio del voucher sociosanitario autismo della regione Lombardia
No full textIl presente contributo, con l’obiettivo di promuovere la diffusione di azioni pedagogico-educative efficaci in materia di autismo, propone l’analisi e la riflessione circa un nuovo modello di presa in carico che si pone lungo il piano
delle traiettorie definite dalle principali indicazioni nazionali e internazionali (ONU, 2006). Oggetto di indagine è il Voucher Sociosanitario Autismo, ossia un progetto avanzato dalla Regione Lombardia per incrementare l’offerta regionale rispetto ai percorsi abilitativi e riabilitativi rivolti alle persone con diagnosi di gravissima disabilità comportamentale autistica (DGR 6003, 2022)
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Colony membership, division of labor, and genetic relatedness among females of colonies of Eustenogaster fraterna (Hymenoptera, Vespidae, Stenogastrinae)
No full textTo compare the social structure of primitively social wasps with that of communal breeding vertebrates, we used a new technique based on micro videocameras applied to the nest envelope to study the organization of behavior in Malaysian colonies of the stenogastrine wasp Eustenogaster fraterna. The reproductive division of labor in this species appears to be different from that reported so far in other species of Stenogastrinae: it is at a very primitive stage because the helpers work less hard and take fewer risks than the egg-layers. Nevertheless, the very low-risk tasks performed by the helpers (nest guarding and supply of abdominal secretion to the young larvae) are important for the colonies. Behavioral characters and ovarian development of the helpers suggest that females are only temporary helpers and that they wait to start their own production of offspring in the hope of inheriting the nest. Females of the same colony can be highly related, but in some colonies we found low-related individuals. The social organization of these wasps resembles that of vertebrate groups with helpers at the den, i.e., in the small number of individuals, the division of labor with limited reproductive inhibition, and the prolonged external work of the reproductive individuals. However, the main drive to sociality in these insects appears to be different, being based mainly on problems connected with short adult life and long immature brood development
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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