1,220 research outputs found
Transfer of fertilized ova in the hamster
No full textThesis (M.A.)--Boston UniversityTransfer of fertilized ova from a donor female to a uterine foster mother can be a valuable tool for attack of problems of gen etics, cancer, embryologJ, growth, immunology, and fertilization. The technique has been practiced in the rabbit, mouse, rat, and sheep with varying degrees of success.
Holding media for the ova usually consist of blood serum, alone, or modified with a balanced saline solution.
Diffusion chambers constructed of Millinore Filter membrane have proved valuable for 'in vivo' and 'in vitro' tissue culture and transnlantation. These chambers serve the useful function of mechanically isolating the contents from surrounding tissues and free cells, while providing diffusion of metabolites and oxygen into the chamber to nourish the enclosed tissue .
Experimental procedure
Donor albino females were mated with albino males. Approximately 24-33 hours after fertilization, when the ova were in the two cell stage, salpingectomies were performed. The ova were removed from the uterine tubes, and transferred to the ovarian bursa of pseudopregnant albino recipients [TRUNCATED
Mucus enhances gut homeostasis and oral tolerance by delivering immunoregulatory signals
No full textA dense mucus layer in the large intestine prevents inflammation by shielding the underlying epithelium from luminal bacteria and food antigens. This mucus barrier is organized around the hyperglycosylated mucin MUC2. Here we show that the small intestine has a porous mucus layer, which permitted the uptake of MUC2 by antigen-sampling dendritic cells (DCs). Glycans associated with MUC2 imprinted DCs with anti-inflammatory properties by assembling a galectin-3-Dectin-1-FcγRIIB receptor complex that activated β-catenin. This transcription factor interfered with DC expression of inflammatory but not tolerogenic cytokines by inhibiting gene transcription through nuclear factor κB. MUC2 induced additional conditioning signals in intestinal epithelial cells. Thus, mucus does not merely form a nonspecific physical barrier, but also constrains the immunogenicity of gut antigens by delivering tolerogenic signals10.1126/science.123791
OVA design with augmented reality
No full textEn la educación estos últimos años hemos se sufrido alzas alzas en las deserciones de los estudiantes de diferentes entidades, estratos y programas de formación. Las Tecnologías de la Información y la Comunicación (TIC), se han implementado para solucionar brechas económicas, políticas, de comunicación y ahora se está acelerando su uso en la educación del país. Ortiz, Cristia. (2015). Tecnologia para mejorar el aprendizaje: ¿Cómo promover programas efectivos?. http://www.eduteka.org/BID-670.php
Se tienen plataformas Learning Managament Software (LMS) con las cuales se amplía la cobertura de la educación a todos los lugares y no se vuelve indispensable estar en un aula convencional para poder adquirir conocimientos, es decir, el conocimiento y las estrategias pedagógicas se están flexibilizando.
Se ve el uso de videoBeam, televisores, programas como prezi, power point y plataformas gestoras de contenido conocidas como son Blackboard, Moodle, etc. Pero aun así es muy baja la efectividad del uso de las TIC, no solo por usar un videoBeam en vez de pasar copias físicas ya se está implementando el uso de las TIC, es indispensable un proceso mucho más profundo de conocimiento de herramientas tecnológicas y la estrategia pedagógica para implementarlas con un resultado exitoso. Fonseca Morales. (2006). Materiales y recursos didácticos, que haríamos sin ellos.http://www.educaweb.com/noticia/2006/05/15/materiales-recursos-didacticos-hariamos-ellos-1233/
En este documento se presenta la investigación desarrollada en la creación de OVA para procesos de formación tanto presenciales como virtuales, se ven OVA con desarrollos de productos multimedia muy enriquecidos, como videos, aplicaciones interactivas en Flash o cualquier otro lenguaje, pero esto se encuentra en páginas de libre acceso como YouTube, Scribd y muchas otras páginas que se indagan en la red.
Esta investigación se basa en implementar la tecnología de Realidad Aumentada en la construcción de un OVA para complementar un proceso de formación en el Servicio Nacional de Aprendizaje – SENA ubicado en la sede de Floridablanca.
Además del desarrollo del prototipo de OVA en realidad aumentada se implementa en un proceso piloto de capacitación con estudiantes para medir su percepción y su efectividad en la construcción de conocimientoIntroducción: 8
1. Planteamiento del problema y justificación 10
1.1 Objetivos 13
1.1.1 Objetivo general 13
1.1.2 Objetivos especificos 13
2. Mrco teorico o estado del arte 14
2.1 Estado del arte 14
2.2 Realidad Aumentada 26
2.2.1 Concepto 26
2.2.2 Características 27
2.2.3 Historia 27
2.2.4 Herramientas 31
2.2.5 Ámbito de Aplicación 40
2.3 Software 3D 41
2.3.1 Conceptos 41
2.3.2 Características 41
2.3.3 librerías 42
3. Metodología 42
4. Resultados 46
4.1 Fase de Análisis 48
4.2. Fase de Diseño 53
4.3. Fase de Código 57
4.4. Fase de Pruebas 63
5. Conclusiones 76
6. Recomendaciones y trabajos futuros: 77
7. Bibliografía 78MaestríaIn education, in recent years we have suffered rises in the dropouts of students from different entities, strata and training programs. Information and Communication Technologies (ICT) have been implemented to solve economic, political and communication gaps and now their use in education in the country is accelerating. Ortiz, Cristia. (2015). Technology to improve learning: How to promote effective programs ?. http://www.eduteka.org/BID-670.php
There are Learning Management Software (LMS) platforms with which the coverage of education is expanded to all places and it is not essential to be in a conventional classroom to acquire knowledge, that is, knowledge and pedagogical strategies are becoming more flexible .
The use of videoBeam, televisions, programs such as prezi, power point and well-known content management platforms such as Blackboard, Moodle, etc. is seen. But even so, the effectiveness of the use of ICT is very low, not only by using a videoBeam instead of passing physical copies, the use of ICT is already being implemented, a much deeper process of knowledge of technological tools and the pedagogical strategy to implement them with a successful result. Fonseca Morales. (2006). Teaching materials and resources, what we would do without them. Http://www.educaweb.com/noticia/2006/05/15/materiales-recursos-didacticos-hariamos-ellos-1233/
This document presents the research developed in the creation of OVA for both face-to-face and virtual training processes, OVAs are seen with developments of highly enriched multimedia products, such as videos, interactive applications in Flash or any other language, but this is found in Free access pages like YouTube, Scribd and many other pages that are investigated on the net.
This research is based on implementing Augmented Reality technology in the construction of an OVA to complement a training process in the National Learning Service - SENA located at the Floridablanca headquarters.
In addition to the development of the OVA prototype in augmented reality, it is implemented in a pilot training process with students to measure their perception and their effectiveness in building knowledgeModalidad Presencia
FIGURE 5. Jasonisis thresheri n. gen., n in A new genus and species of the family Isididae (Coelenterata: Octocorallia) from a CMAR Biodiversity study, and a discussion on the subfamilial placement of some nominal isidid genera
No full textFIGURE 5. Jasonisis thresheri n. gen., n. sp., holotype: A, a polyp with small ova; B, polyp head close-up (a = aggregations of sclerites below base of tentacles; b = naked polyp surface); C, a polyp containing large ova; D–E, polyp body wall closeups. (All light microscope images).Published as part of Alderslade, Philip & Mcfadden, Catherine S., 2012, A new genus and species of the family Isididae (Coelenterata: Octocorallia) from a CMAR Biodiversity study, and a discussion on the subfamilial placement of some nominal isidid genera, pp. 21-39 in Zootaxa 3154 (1) on page 29, DOI: 10.11646/zootaxa.3154.1.2, http://zenodo.org/record/524688
Circumventing cellular immunity by miR142-mediated regulation sufficiently supports rAAV-delivered OVA expression without activating humoral immunity
No full textFull author list omitted for brevity. For the full list of authors, see article.Recombinant adeno-associated virus (rAAV)-mediated gene delivery can efficiently target muscle tissues to serve as "biofactories" for secreted proteins in prophylactic and therapeutic scenarios. Nevertheless, efficient rAAV-mediated gene delivery is often limited by host immune responses against the transgene product. The development of strategies to prevent anti-transgene immunity is therefore crucial. The employment of endogenous microRNA (miRNA)-mediated regulation to detarget transgene expression from antigen presenting cells (APCs) has shown promise for reducing immunogenicity. However, the mechanisms underlying miRNA-mediated modulation of anti-transgene immunity by APC detargeting are not fully understood. Using the highly immunogenic ovalbumin (OVA) protein as a proxy for foreign antigens, we show that rAAV vectors containing miR142 binding sites efficiently repress co-stimulatory signals in dendritic cells, significantly blunt the cytotoxic T cell response, allow for sustained transgene expression in skeletal myoblasts, and attenuate clearance of transduced muscle cells in mice. Furthermore, the blunting of humoral immunity against circulating OVA correlates with detargeting of OVA expression from APCs. This demonstrates that incorporating APC-specific miRNA binding sites into rAAV vectors provides an effective strategy for reducing transgene-specific immune response. This approach holds promise for clinical applications where the safe and efficient delivery of a prophylactic or therapeutic protein is desired
Inhibition of () IL-5 and () IL-13 and induction of () IL-12 and () IFN-γ production in OVA-sensitized BALB/c mouse spleen cell cultures by IMOs and or control
No full text<p><b>Copyright information:</b></p><p>Taken from "Novel oligodeoxynucleotide agonists of TLR9 containing N-Me-dC or N-Me-dG modifications"</p><p>Nucleic Acids Research 2006;34(11):3231-3238.</p><p>Published online 23 Jun 2006</p><p>PMCID:PMC1904100.</p><p>© 2006 The Author(s)</p> Spleens were obtained from BALB/c mice sensitized with OVA as described in Materials and Methods. Single spleen cells were incubated with 100 μg/ml OVA in the absence or presence of 140 nM IMO for 72 h, and the cytokine levels in culture supernatants were measured using ELISA. The results represent the mean cytokine concentration from triplicate wells ± SD
Effects of various cooking conditions on acrylamide formation in rolled patty
No full textIn this study on acrylamide formation, the effects of the type of frying oil, frying period and covering with egg during frying of a rolled patty (a traditional Turkish carbohydrate-rich food) were investigated. The differences between frying periods were statistically significant for each oil (p 0.90) was found between L (light) values and acrylamide amounts.Food Department and Environment Department in the National Chemical Laboratory in Athens, GreeceThe author wishes to acknowledge the support of the Food Department and Environment Department in the National Chemical Laboratory in Athens, Greece. for the analysis of acrylamide: and Professor Dr Yasar Hisil for helping in GC-MS applications
Modifying Antigen-Encapsulating Liposomes with KALA Facilitates MHC Class I Antigen Presentation and Enhances Anti-tumor Effects
No full textFor a successful anti-cancer vaccine, antigen presentation on the major histocompatibility complex (MHC) class I is a requirement. To accomplish this, an antigen must be delivered to the cytoplasm by overcoming the endosome/lysosome. We previously reported that a lipid nanoparticle modified with a KALA peptide (WEAKLAKALAKALAKHLAKALAICALICA), an alpha-helical cationic peptide, permits the encapsulated pDNA to be efficiently delivered to the cytoplasm in bone marrow derived dendritic cells (BMDCs). Herein, we report on the use of KALA-modified liposomes as an antigen carrier, in an attempt to induce potent antigen-specific cellular immunity. The subcutaneous injection of KALA-modified ovalbumin (OVA)-encapsulating liposomes (KALA-OVA-LPs) elicited a much more potent OVA-specific cytotoxic T lymphocyte activity and anti-tumor effect in comparison with particles that were modified with octa-arginine (R8), a cell-penetrating peptide (R8-OVA-LPs). In addition, the numbers of OVA-specific CD8(+) T cells were increased by immunization the KALAOVA-LPs. The treatment of BMDCs with KALA-OVA-LPs induced a substantial MHC class I antigen presentation. Furthermore, the acidic pH-dependent membrane destabilization activity of KALA-OVA-LPs strongly suggests that they are able to escape from endosomes/lysosomes and thereby deliver their cargos to the cytoplasm. Collectively, the KALAmodified liposome is a potential antigen delivery platform for use as a protein vaccine
Whipworms in humans and pigs: origins and demography
No full text© 2016 Hawash et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0
International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and
reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to
the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver
(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. The attached file is the published version of the article.NHM Repositor
Dual stimulation of antigen presenting cells using carbon nanotube-based vaccine delivery system for cancer immunotherapy
No full textAbstractAlthough anti−cancer immuno−based combinatorial therapeutic approaches have shown promising results, efficient tumour eradication demands further intensification of anti−tumour immune response. With the emerging field of nanovaccinology, multi−walled carbon nanotubes (MWNTs) have manifested prominent potentials as tumour antigen nanocarriers. Nevertheless, the utilization of MWNTs in co−delivering antigen along with different types of immunoadjuvants to antigen presenting cells (APCs) has not been investigated yet. We hypothesized that harnessing MWNT for concurrent delivery of cytosine−phosphate−guanine oligodeoxynucleotide (CpG) and anti-CD40 Ig (αCD40), as immunoadjuvants, along with the model antigen ovalbumin (OVA) could potentiate immune response induced against OVA−expressing tumour cells. We initially investigated the effective method to co−deliver OVA and CpG using MWNT to the APC. Covalent conjugation of OVA and CpG prior to loading onto MWNTs markedly augmented the CpG−mediated adjuvanticity, as demonstrated by the significantly increased OVA−specific T cell responses in vitro and in C57BL/6 mice. αCD40 was then included as a second immunoadjuvant to further intensify the immune response. Immune response elicited in vitro and in vivo by OVA, CpG and αCD40 was significantly potentiated by their co−incorporation onto the MWNTs. Furthermore, MWNT remarkably improved the ability of co−loaded OVA, CpG and αCD40 in inhibiting the growth of OVA−expressing B16F10 melanoma cells in subcutaneous or lung pseudo−metastatic tumour models. Therefore, this study suggests that the utilization of MWNTs for the co−delivery of tumour−derived antigen, CpG and αCD40 could be a competent approach for efficient tumours eradication
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