41,663 research outputs found
CARATTERIZZAZIONE FUNZIONALE DEI MUTANTI DEL RECETTORE PER LA TIREOTROPINA (TSH)
No full textContext: Heterozygous mutations in the TSH receptor gene (TSHR) are associated with partial TSH
resistance, characterized by isolated nonautoimmune hyperthyrotropinemia (NAHT). The prevalence
and management of this condition is controversial.
Objective: Our objective was to investigate the prevalence and clinical impact of TSHR alterations
in a large series of pediatric patients with NAHT and to dissect their mechanism of action.
Design and Setting: For this prospective multicenter study, clinical data and samples were collected
in the clinical units and conveyed to a centralized laboratory for analysis.
Patients: Subjects included 284 unrelated patients with NAHT aged 18 yr. Exclusion criteria
included thyroid dysgenesis or major associated congenital defects.
Main Outcome Measures: Parameters of thyroid function, TSHR gene analysis, and TSHR functional
assays were evaluated.
Results: The frequency of heterozygous nonpolymorphicTSHRvariationswas11.8%.Weidentified
seven previously unknown variations: a frameshift (p.Q33PfsX46), two intronic (g.IVS4+ 2A<G, IVS4-1T<G),
and nine novel missense (p.P162L,p. P160S, p.Y466C, p.I583T, p.I607T,p.L57P, p.P668L, p.S745C and p.R609Q) variations. The missense
variations variably affected TSHR membrane expression and Gs and/or Gq/11 signaling. Several
variations cosegregated with NAHT in the affected families. Parameters of thyroid function were
similar between affected and unaffected family members.
Conclusions: Nonpolymorphic alterations in the TSHR gene are commonly associated with isolated
NAHT in young patients, thus configuring partial TSH resistance as the most frequent inheritable
cause of isolated NAHT. The identification of TSHR defects may thus be helpful for a tailored
management of subclinical hypothyroidism. We provide further evidence that besides the wellknown
defects inGs signaling, TSHR genetic alternations found in NAHT may frequently impair the
Gq/11 pathway
"Il Calcare a Lucina pomum”della Successione Epiligure dell’Appennino modenese: stratigrafia, sedimentologia e dati geochimici.
No full textIn this paper "Lucina limestones", outcropping in the Modena area, where for the first time were found, are investigated from a stratigraphical, sedimentological and geochemical viewpoint. The data collected allow these deposits to be ascribed to the Termina Formation of the Epiligurian Sequence and to be dated at the top of the Upper Serravallian. Different outcrop types are recognized: 1) marly-calcareous lenses with lucinids, 2) marly-calcareous and calcarenitic hectometric levels with lucinids, 3) marly-calcareous breccias with lucinids. The first two outcrop types clearly show show evidence of a primary deposition and gradually pass to surrounding pelitic marls, whereas the third one is resedimented. The sedimentological and stratigraphical results obtained from this research permit to differentiate the numerous "Lucina deposits" reported in literature. Geochemical and taphonomic analyses confirm the stratigraphical and sedimentological data and lead to interpret the lucinid assemblages of the Modena territory deposits as chemosynthetic communities related to hydrocarbon sources. A methanogenic hypothesis, different from those commonly reported in literature, is here proposed which considers lucinid chemoherm as derived from local bacterical oxidation of marly-pelitic sediment
Study of the Modulation of Cytokine Release by Natural Compounds with Pharmacological Properties Using Cell-Based Systems
No full textBackground: The screening of the pharmacological properties of natural compounds (i.e., anti-inflammatory effects) may take advantage of some specific cell-based systems. Parthenolide (PTN) and Copaifera langsdorfii (Copaiba) are natural compounds used to prevent and treat headache and migraine and in inflammatory diseases involving respiratory airways, genital-urinary apparatus and
skin, respectively, but their effects at the cellular level are poorly understood. Methods: Mouse BV-2 microglia and human THP-1 monocyte cell lines were used. The nuclear translocation of nuclear factor (NF)-kB was evaluated by Western blotting analysis. The secretion of inflammatory cytokines (interleukin (IL)-1β, IL-6, tumor necrosis factor-α (TNFα)) was evaluated by immunometric assays (ELISA).
Results: Treatment of BV-2 cells with 1 μM PTN and of THP-1 cells with 10 μM Copaiba oleoresin (OR), containing diterpene acids, diterpenes and sesquiterpenes, strongly reduced the NF-kB translocation to the cell nucleus induced by 1 μg/mL
lipopolysaccaride (LPS). In BV-2 cells, PTN reduced IL-6 secretion in a dosedependent manner (-29% at 200 nM, P < 0.001; -45% at 1 μM, P < 0.001; -98% at 5
μM, P < 0.001; ANOVA). Moreover, at 5 μm (highest concentration tested) PTN also reduced TNF-α secretion (-54%, P < 0.001). Preincubation of LPS-stimulated THP-1 monocytes with OR (dose-range: 0.1-10 mM), reduced the release of all tested
cytokines (IL-1β, IL-6, TNF-α). Conclusions: The results obtained provide strong evidence that both cell-based models are useful to validate the anti-inflammatory properties of PTN and OR at the cellular level and suggest that they are related to inhibition of cytokine secretion and NF-κB nuclear translocation
Cap.6 - I fattori di crisi dei controlli nelle aziende di credito
No full textIl lavoro ha l’obiettivo di evidenziare i risultati di una ricerca avente ad oggetto i sistemi di controllo nelle banche, con segnato riferimento alle casistiche del suo fallimento e alle possibili cause ad esso relative, anche alla luce dell’evoluzione dell’operatività degli intermediari e delle variazioni nella struttura dei sistemi finanziari. In particolare, si delineano le attività di controllo tipiche del settore del credito declinando un modello di sistema di controlli per le banche e riportando, contestualmente, le evidenze emerse nell’esperienza di vigilanza e nella letteratura sul tema della crisi dei controlli nel settore oggetto di indagine. Sono poi presentate le risultanze della somministrazione di un questionario ad alcuni componenti di organi di controllo e gli elementi qualificanti emersi in alcune interviste di taglio qualitativo condotte ad attori chiave del controllo nelle banche italiane. L’analisi dei questionari somministrati e delle interviste condotte risulta utile per fornire spunti circa i punti di forza e di debolezza e le possibili azioni di miglioramento dei sistemi di controllo in concreto implementati dalle banche italiane
Unsaponifiable fraction of unripe fruits of Olea europaea : An interesting source of anti-inflammatory constituents
No full textThe unsaponifiable fraction of olive oil from unripe fruits of Olea europaea at different stages of maturation (from 20 to 32 weeks after flowering) was analyzed by gas chromatography-mass spectrometry in order to select the time associated to the unsaponifiable fraction with the maximal yield in bioactive constituents. According to quantitative gas chromatography-mass spectrometry analysis, the unsaponifiable fraction (2.46 % of the total oil) from olive fruits at the 22nd week was found to contain the maximal yield in anti-inflammatory constituents. Its composition was lanosterol (2.60 mg/g oil), stigmasterol (2.15), cycloartanol acetate (2.04), stigmastan-3,5-diene (2.01), obtusifoliol (1.93), cholesta-4,6-dien-3-one (1.42), α-amyrin (1.42), α-tocopherol (1.32), squalene (1.02), β-amyrin (0.57), and β-sitosterol (0.22). At later times, there was a decrease in the quantitative unsaponifiable fraction yield and a qualitative shift in the bioactive constituents. The 22nd week unsaponifiable fraction was subsequently incorporated into a topical preparation to be utilized for a small pilot clinical study in five patients affected by osteoarthrosis. According to clinical observation, the application of the ointment (three times daily for three weeks) attenuated hand and knee joint inflammatory features in all patients and was not associated to any adverse reactions
GC–MS characterisation and biological activity of essential oils from different vegetative organs of <i>Plectranthus barbatus</i> and <i>Plectranthus caninus</i> cultivated in north Italy
No full text<div><p>Essential oils (EOs) from the roots, stems and leaves of <i>Plectranthus barbatus</i> (A) and <i>Plectranthus</i><i>caninus</i> (B), cultivated in north Italy, were obtained by steam distillation and chemically characterised by gas chromatography–mass spectrometry. The highest yields were obtained from roots (268.15 and 673.60 mg/kg from A and B), followed by leaves (64.34 and 26.65 mg/kg) and stems (19.76 and 18.63 mg/kg). A total of 128 structures were identified in A and 121 in B. Fe<sup>++</sup> chelating and antiradical activities (DPPH and ABTS) were evaluated: root and stem EOs showed the strongest activities, while EOs from leaves did not show relevant activities. All EOs were tested for their <i>in vitro</i> antimicrobial activity, showing optimal growth-inhibition in antibiogram (∅>35 mm) and MIC tests (32–64 μg/mL) against <i>Candida albicans</i>, while EOs from leaves of both species showed a good activity (25 < ∅ < 34 mm, MIC 64–128 μg/mL) against <i>Escherichia coli</i>.</p></div
LABORATORIO ITALIA Esposizione d’Architettura 2005 – Parma, nell’ambito del secondo Festival dell’Architettura di Parma, chiesa di S. Lodovico, Parma 19-25 settembre 2005
No full texthttp://www.floornature.it/laboratorioitalia/index.html
Progettisti invitati ad esporre:
Sezione Genealogie (co-curatori L. Amistadi e D. Chizzoniti):
Anselmi – Avallone & Pierluisi, FTFG Studio, L. Montuori, Scape
Aymonino – V. Longheu, E. Pitzalis
G. Canella – E. Bordogna, D. Chizzoniti, G. Fiorese,
P. Culotta – G. Guerrera, A Sciascia
V. Gregotti – W. Noebel, S. Pascolo, E. Puglielli
Isola – Baietto & Battiato & Bianco, Bruna & Mellano, G. Drocco
Monestiroli – F. Bucci, M. Ferrari, C. Moccia, R. Neri
A Natalini – M. e F. Arrigoni, Guicciardini & Magni, M. De Lucchi, D. Palterer, G. Renzi
N. Pagliata - G. Di Domenico, FGP Studio, S. Raffone
G. Polesello – A. Dal Fabbro
P. Portoghesi – P. Bernizza, M. Durastanti, D. Scatena, G. Priori, L. Ribichini
Purini – Modulo Quattro, G. Neri, R. Partenope, Menegatti & Nencini
L. Semerani – A. Gallo, G. Marras, G. Fraziano
F. Venezia - S. Fierro, V. Latina, R. Lopes, B. Messina
Vitale – P. Cofano, L. Gemini, A. Lorenzi, C. Piva
Sezione Professione e ricerca (co-curatori E. Mucelli, A. Trentin, S. Rossl)
5+1 (P. Arbocò, P. Feltri, A. Femìa, G. Peluffo, M. Vallino), ABDR (M. L. Arlotti, M. Beccu, P. Desideri, F. Raimondo), Albori Associati(E. Almagioni, G. Borella, F. Riva), B. Albrecht, ARCHEA, S. Boeri, C. Botticini, M. Bradaschia, A. Bucci, C+S (C. Cappai, M. A. Segantini), M. Ciarlo, P. Ciorra/M. Perriccioli, A. Citterio, Corvino & Multari, R. Cutillo (OfCASTUDIO), M. De Vita, E. Di Franco, A. Ferlenga, M. Galantino, C. Gambardella, GMA (F. Garofalo, S. Y. Miura), G. Goffi, IPOSTUDIO (L. Celle, R. Di Giulio, C. Terpolilli, E. Zanasi Gabrielli, P. Cionci); Cristofani & Lelli, V. Melluso, n!Studio (S. Ferrini, A. Stella), NEMESI (M. Molè), Netti Architetti (L. Netti, G. A. Valente), F. Nonis, C. A. Maggiore, P. C. Pellegrini, R. Platania, C. Quintelli, M. Rendina, Ricci & Spaini, I. Rota, M. Saito, B. Servino, Studio 4, G. Vaccarini, D. Vargas, VULCANICA (M. Borrelli, E. Borrelli, A. M. Di Chio)
Sezione Nuovi Laici (co-curatori D. Cogliandro, L. Gelmini, D. Lama, R. Maddaluno)
PIEMONTE-LIGURIA-SARDEGNA (a cura di L. Gelmini): A12, Amoretti & Calvi, Baracco & Wright, C+C04, F. Leoni, OBR, R. Rosset,
LOMBARDIA (a cura di L. Gelmini): Belloni-Brazis; C. Bigi/F. Della Torre, Bonforte/Giorni, N. Braghieri, E. Caravatti; R. Ciravolo, Greppi/Bianchetti, Guidarini/Salvadeo, Studio azero (P. Mestriner), Liverani-Molteni, C. Nozza, A. Scandurra,
VENETO-TRENTINO-FRIULI (a cura di L. Gelmini): Cerchia & Mentil, Donner & Sorcinelli, E. Franco, MADE, M. Manzelle, L. Peretti, V. Tronchin
EMILIA ROMAGNA (a cura di L. Gelmini): E. Cerasi; M. Contini; D. Costi; Iotti & Pavarani; Oliva-Prandi-Zilioli; A. Ravalli; Ufficio Progetti (G. A. Bertani, F. Vezzali)
TOSCANA – MARCHE - ABRUZZO (a cura di L. Gelmini): A. Boschi, M. D’Annuntiis, MDU, M. Meossi, A. Milani, n'tt, PAO, D. Raponi (HOV), S. Re
LAZIO (a cura di L. Gelmini): E. D'Amato, Doppiomisto, Ian+, LABICS, ma0, Studio Seste, Tran(‘)city
CAMPANIA (a cura di D. Lama): L. Capobianco, L. Centola, F. Costanzo/L. Lanini, S. D’Ascia, Gnosis, M. Russo, Suburbia, Tecla
PUGLIA (a cura di R. Maddaluno): M. Alvisi, E. Ampolo, V. Ardito, E. Bruschi/A. Esposito, D. Cara, A. Foresta, N. Gibertini, L. Labate, M. Montemurro
SICILIA-CALABRIA (a cura di D. Cogliandro): C. Agostino, BOMA, IKON, Map, G. Merendino, V.A. Morabito, Monoarchitetti, N. Piazza, M. Sestito, Sud’arc-
Air dispersed essential oils combined with standard sanitization procedures for environmental microbiota control in nosocomial hospitalization rooms
No full textObjective: Environmental bacterial contaminant microorganisms are an ongoing problem in hospitals. Essential oil vapours (EO) may help reducing this type of contamination. Aim of this study was to evaluate the efficacy of nebulized selected essential oils (EO) in reducing the microbial contamination in residential health care house rooms. Design: The study was carried out in a two-story 112-bed tertiary care structure (approximately 1060 m2). Contamination in rooms and corridors was monitored for a total of n = 5 months, including a starting baseline sampling and one end-study point, and without combined treatment (standard sanitization alone). Contact slides were collected for microbiological analysis. Results: Reductions in both bacterial and fungal contamination were observed between rooms cleaned using standard sanitization alone or in combination with essential oils nebulization (average 90% decrease for total count, P < 0.01; 90% for yeasts and molds, P < 0.05). Decreases of antibiotic (70%), mucolytic (100%), bronchodilators (100%), and steroidal (67%) and non-steroidal anti-inflammatory drugs (33%) prescriptions were observed, with no adverse effects on patients. Conclusions: The selected EO composition is effective in reducing both the environmental microbial contamination and pharmaceutical drugs consumption in a nosocomial health care house. This study demonstrates that aerial EO diffusion combined with standard sanitization procedures, has great potential to reduce the microbial contamination in critical hospital environments such as hospitalization rooms
Evaluation of bio-psycho-social frailty in older persons on the territory: The method and the experience of the ‘medesano health house’
No full textBackground and aim of the work: Frailty in older people is a situation of risk of dependency caused by many factors. On this consideration, the Medesano city started a survey on the condition of fragility of people >75 aimed at building a monitoring system for timely interventions on the factors of risk and implementation of measures for preventing dependency. Methods: The survey was conducted jointly by the family doctor and the social worker in the City. The multidimensional assessment considered logistic and housing conditions, disease, medications, basic and instumental acitvities of daily living, neurological and psycological conditions. Results: The survey included 761 older people, with a following definition of condition and levels of frailty both at social (loneliness, lack of supporting network, etc..) and health level (complexity of the regimen, high and/or severe comorbidity) was planned from the beginning as the basis on which developing a continuous in time monitoring program for assessing the frailty, for a early taking in charge by Health and Welfare services, boosting integrated health and welfare interventions and promoting networks of solidarity and sociality. Conclusion: The data seem to confirm that the methodology used is sufficiently adequate to intercept frailty in > 75 years unknown to social services indicating that the monitoring on the functionality of the subjects obtained from the surveys by the General Practitioners could be deemed as a privileged way for a survey on large populations. After the survey was activated continuous monitoring of patients with social and clinical frailty. © Mattioli 1885
Nicotinic and M1-M2 muscarinic cholinergic control of ACTH response to insulin-induced hypoglycemia in man
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