122,565 research outputs found
Gel electrophoresis and immunoblotting for the detection of casein proteolysis in cheese
No full textThe whole N fraction of six samples of hard and semi-hard pressed cheeses was analysed using PAGE, polyacrylanzide gel isoelectric focusing and immunoblotting with polyclonal antibodies against beta- and alpha(s1)-casein. The origin of some electrophoretic bands corresponding to peptides produced from the enzymic degradation of the casein fractions was established. A number of these peptides were also present in the in vitro hydrolysates of casein with plasmin and chymosin. Thus, it was also possible to determine which casein was the source of each peptide and which enzymes were active in cheese. Compared with the traditional Coomassie staining procedures, immunoblotting is more sensitive and specific, making the interpretation of each electrophoretic profile easy. Thus, it was also possible to obtain a clear picture of the state of each casein fraction in a cheese variety. Two main peptides were isolated from the pH 4.6-insoluble N fraction of Parmigiano-Reggiano using DEAE-cellulose chromatography and identified, from the amino acid sequence of the N- and C-terminal ends, as gamma(3)-casein ( (beta-casein(f108-209)) and alpha(s1)-PL1 (alpha(s1)-casein(f80-199). In both cases, a Lys-X bond was hydrolysed, indicating the action of a trypsin-like enzyme in beta- and alpha(s1)-casein hydrolysis during the ripening of this variety of hard pressed cheese
Solving the PnP Problem with Anisotropic Orthogonal Procrustes Analysis.
No full textIn this paper we formulate the Perspective-n-Point (a.k.a. exterior orientation) problem in terms of an instance of the anisotropic orthogonal Procrustes problem, and de- rive its solution. Experiments with synthetic and real data demonstrate that our method reaches the best trade-off be- tween speed and accuracy. The MATLAB code reported in the paper testifies that it is also exceedingly simple to im- plement
Image localization and parsing using 3D structure
No full textL'obiettivo di questa tesi è lo studio di due problematiche fondamentali della visione computazionale: la localizzazione direttamente da immagini e la segmentazione semantica di un'immagine.
Il primo contributo di questa tesi è lo sviluppo di un sistema che calcola un'accurata e rapida localizzazione di una fotocamera portatile, utilizzando oltre ad un dataset di immagini pre-registrate, dati tridimensionali ottenuti da una ricostruzione mediante un algoritmo di "Structure from Motion".
L'informazione 3D viene considerata sotto due differenti aspetti: in primo luogo essa è direttamente coinvolta nella fase di registrazione della camera da cui si ottengono robuste corrispondenze di tipo 2D-3D invece di coppie di punti salienti 2D-2D; inoltre il sistema, nella sua fase di reperimento di immagini similari, sfrutta la fase di clusterizzazione dell'algoritmo di "Structure from Motion" migliorando la propria efficienza e robustezza.
La seconda parte della tesi consiste in una analisi dettagliata di uno dei componenti principali del sistema di localizzazione, l'algoritmo di stima della posa della camera partendo da corrispondenze 2D-3D. In particolare viene presentata una nuova formulazione del problema, in letteratura indicato come "Perspective-n-Point problem" o "exterior orientation problem", trasformandola in un'istanza di un problema di analisi di Procrustes di tipo anisotropico ortogonale.
Il contributo finale della tesi è lo sviluppo di un nuovo approccio per la segmentazione semantica di immagini in contesto urbano che coinvolge anch'esso in maniera preponderante la struttura tridimensionale ottenuta dall'algoritmo di "Structure from Motion" in termini di trasferimento di etichette semantiche da un'immagine precedentemente annotata all'immagine di test.
L'immagine da annotare può essere sia un'immagine appartenente al dataset di partenza dell'algoritmo di "Structure from Motion" a cui non è stata associata nessuna informazione semantica, sia un'immagine esterna al dataset che è stata appena registrata mediante il sistema di localizzazione precedentemente descritto.
L'assegnamento delle etichette è modellato mediante un Markov random field i cui nodi sono superpixel estratti dall'immagine di test.The aim of this thesis is the study of two fundamental problems in computer vision: localization from images and semantic image segmentation. The first contribution of this thesis is the development of a complete system that obtains an accurate and fast localization of a hand-held camera device, leveraging not only on a dataset of registered images but also on the three-dimensional information obtained by a Structure from Motion reconstruction.
We exploit the 3D structure under two different aspect: first it is directly involved in the camera registration making available robust 2D-3D correspondences instead of 2D-2D pairs of matched features, furthermore we take advantage of the image clustering computed in the Structure from Motion algorithm during the retrieval step of the localization system improving both robustness and efficiency of the aforementioned algorithmic stage.
The second part of the thesis consists in an in-depth analysis of one of the main components of the localization system, the camera pose estimation from 2D-3D correspondences. In particular we present a novel formulation of the Perspective-n-Point problem, also known as exterior orientation, in terms of an instance of anisotropic orthogonal Procrustes problem.
The last contribution of the thesis is the proposal of a new approach to semantic image segmentation in urban environment that deeply involves the Structure from Motion 3D structure in terms of label transfer from a pre-labeled image to a query image. The query image can be whether an image belonging to the SfM dataset that does not have any semantic information or an external image that has just been localized by the localization system aforementioned. The label assignment problem is modeled as a Markov random field where the nodes are the superpixels of the query image
Very late thrombosis in acute myocardial infarction: drug-eluting versus uncoated stents
No full textTo evaluate clinical outcomes at 24 months in an unselected population with ST-segment elevation myocardial infarction (STEMI) undergoing percutaneous coronary intervention (PCI) with drug eluting stents (DES), as compared with bare metal stents (BMS)
The effects of chronic ethanol consumption on carcinogen metabolism and on O6-methylguanine transferase-mediated repair of alkylated DNA.
No full textA Multi-Language Comparison of Influences on Author Verification using Character N-Grams
No full textWe create a new multi-language corpus for author verification based on Wikipedia talkpages, and evaluate the influence that differences in topic and time have on character n-gram author profiles. Topic alignment between two texts is found to increase author verification precision, and an authors writing style is found to change over time, but not more significantly after 3 years than after 1 year.Information ArchitectureWISElectrical Engineering, Mathematics and Computer Scienc
Appropriate Similarity Measures for Author Cocitation Analysis
No full textWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
The vanishing author in computer-generated works: a critical analysis of recent Australian case law
No full textAbstract
The use of software is ubiquitous in the creation of many copyright works, yet the requirement in copyright law that every work have a human author who engages in independent intellectual effort means that its use may prevent copyright subsistence. Several recent Australian cases have refocused attention on authorship as an essential criterion of copyright subsistence, and these cases suggest that much computer-produced output may be authorless and thus lack copyright protection. This article, the first in a two-part series, analyses how each case deals with the question of authorship of computer-produced works and why the use of software diminishes copyright protection for a significant number of computer-generated works. The article critiques the application of conventional notions of human authorship developed in the pre-computer age to modern productions and suggests alternative approaches to authorship that satisfy both the major objectives of copyright policy and the need to adapt to the computer age. The article argues that, without a broader judicial approach to authorship of computer-generated works, Parliament must remedy the lacuna in protection for these ‘authorless’ works. Possible solutions for reform are suggested. In a forthcoming article, the author comprehensively examines those reform proposals
La renuncia como alternativa al mundo hegemónico en “Era Mercurio”, de Elena Garro
No full textn “Era Mercurio”, I propose the act of renunciation as a resistance to the hegemonic world. This is an appropriation of Javier, the protagonist and narrator, from two irruptions: the diary about the abdication of Carlos A. Madrazo and Mercurio, a supernatural character. These are also intertextualities of the culture represented in the story (1965) and greco-roman culture. Such invasions happen one after the other to oppose Ema (his fiancée), who represents reality and a false beauty, and the social values (contracts) of Mexico City, in this case marriage.Se propone la renuncia como una resistencia ante el mundo hegemónico en “Era Mercurio” (1965), relato de Elena Garro. A este acto recurre Javier, el protagonista y narrador del cuento, a partir de dos irrupciones: el titular de un periódico que anuncia la abdicación de Carlos A. Madrazo, y la presencia de Mercurio, un personaje sobrenatural. Ambas constituyen también intertextualidades referentes a la política mexicana y al mundo grecolatino. Tales invasiones suceden una tras otra para oponerse a Ema (la prometida de Javier), que representa la realidad y una falsa belleza, y a los valores (contratos) sociales del Distrito Federal, en este caso, el matrimonio
Diffusive author(s), cohesive author: Analysis of S/N (1994)
No full textThis study indicates the ways in which various aspects of the author(s) are brought forth in Dumb type’s performance art, the S/N production. Previous research has suggested a non-hierarchical organization of Dumb type and the absence of a “privileged author” in Dumb type’s collaborative work, S/N. However, the results that I have investigated from member’s interviews on the creative process of S/N along with my analysis of the recorded images of S/N, indicate a different aspect of the author(s). First, S/N was created through, so to speak, the collective ideas of the members of Dumb type. Further, S/N has at least nine quotations from previous performances, installations, and printed writings, besides the work-in-progress technique. Explicating one of the “author functions” as given by Michel Foucault, each text has plural subjects of the author. However, it has been revealed from members’ interviews that Teiji Furuhashi had a decision-making role in selecting the members’ ideas within the performance. Since then, S/N has had plural subjects of creation; however, Furuhashi is one of the subjects of creation along with the “privileged author.” S/N has plural authors (diffusive authors) yet at the same time, it has a “privileged author,” Teiji Furuhashi (cohesive author)
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